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Studies on the Relationship of Moisture Content to Threshability and Viability of Pea SeedsRoquia Y Dulalas, Filixberto 01 May 1960 (has links)
When in commercial practice seed peas (Pisum sativum) are harvested, the vines are cut and windrowed or bunched and allowed to cure for a week or longer, depending upon the moisture content of the crop and weather conditions. The sooner the crop can be threshed, the less is the chance of loss caused by rain and the often-necessary attendant operation of turning the windrow or bunch to facilitate drying. In general, growers wait until the vines, pods, and seeds are quite dry. To what extent this is necessary is not known.
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Molecular characterization of threshability genes in wheatSood, Shilpa January 1900 (has links)
Doctor of Philosophy / Genetics Interdepartmental Program / Bikram S. Gill / Threshability is an important agronomic trait in wheat as free-threshing forms facilitate mechanical threshing of grain. All wild relatives of wheat have tough glumes and are non-free-threshing, whereas most cultivated wheats have soft glumes and are free-threshing. Two genetic loci are known to govern the threshability trait in bread wheat. The Q gene located on chromosome 5AL and glume tenacity genes located on homoeologous group-2 chromosomes seem to interact to produce a free-threshing phenotype. Although, the Q gene was found to be a member of APETALLA 2 (AP2) class of transcription factors, the molecular nature of the tough glume genes remains unknown. In the present study, genetic and molecular characterization of two of the threshability genes in wheat was undertaken. The soft glume (sog) gene of diploid wheat and tenacious glume (Tg) gene of hexaploid wheat were characterized and mapped on short arm of chromosome 2Am and 2D respectively. Comparative mapping of sog and Tg genes suggested their independent origins. The sog gene was mapped in a low-recombination region near the centromere on 2AmS. Genomic targeting using deletion bin mapped ESTs assigned the Tg gene to a 4.9 cM interval in the distal 16% of short arm of chromosome 2D. In order to find additional markers for fine-mapping the Tg gene, macrocolinearity between rice and wheat was explored in the Tg region. Although synteny between rice and wheat was found to be conserved in the distal region of chromosome 2DS, the genomic region encompassing the Tg gene in wheat showed some rearrangements relative to rice. Molecular characterization of ethyl methanesulfonate-induced free-threshing mutants in two different non-free-threshing backgrounds revealed point mutations as well as variable sized deletions at Tg locus. Targeting of Tg to the high-recombination gene-rich region in wheat and availability of several genomic resources from the present study will aid in the cloning and further characterization of this important agronomic gene.
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Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markersMalihipour, Ali 26 October 2010 (has links)
Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat.
A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease.
Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive.
The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.
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Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markersMalihipour, Ali 26 October 2010 (has links)
Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat.
A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease.
Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive.
The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.
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Druscheignung als zentrale Führungsgröße im ErntemanagementKlüßendorf-Feiffer, Andrea 12 August 2009 (has links)
Beim Parameter „Druscheignung“ eines Bestandes wird gemeinhin angenommen, dass dieses Kriterium durch die Genetik der Sorte und den Witterungsverlauf, weitgehend unbeeinflusst von Landwirt, festgelegt ist. Und dennoch verändert der Landwirt mit all seinen Entscheidungen von der Auswahl der Sorten, über die Düngung, den Pflanzenschutz bis hin zum Erntemanagement die Druscheignung stetig. Zur Ernte, als letzten Abschnitt der Verfahrenskette, entfaltet die Druscheignung dann außerordentlich große ökonomische Auswirkungen. Anhand verschiedener Beispiele aus Züchtung, Pflanzenernährung, Pflanzenschutz und Erntetechnologie wurde dargestellt, wie auf die Druscheignung Einfluss genommen werden kann und wie diese Auswirkungen monetär zu bewerten sind. Aus dem Bereich der Züchtung wurde die Entwicklung eines neuen Wuchstyps bei den Rapshalbzwergen ausgewählt, der mit weniger Biomasse konkurrenzfähige Erträge erzielt. Die Abreife ist einheitlicher, der Erntetermin kann problemloser fixiert werden, der Drusch ist leistungsstark und verlustarm. Späte und intensiv geführte Sorten sind mit Hilfe einer Sikkation zeitlich früher und leichter zu beernten. Das schafft Erntesicherheit bei geringeren Verlusten, höheren Mähdrescherleistungen und sinkendem Kraftstoffverbrauch. Die bedarfsgerechte Ausbringung des Stickstoffs in Art, Menge und Zeit, entsprechend der kleinräumigen Heterogenität eines Schlages, führt zu einer Homogenisierung der Bestände. Die Bestände reifen gleichmäßiger ab und führen zu etwa 20 Prozent höherer Mähdrescherleistung sowie geringerem Kraftstoffverbrauch. Am Beispiel des Hochschnitts wurde verdeutlicht, wie sich die bessere Beerntbarkeit auf Maschinenkosten, Gesamternteverluste, Qualität und Trocknung auswirkt. Hebt man die Stoppellänge um 10 Zentimeter an, lässt sich die Mähdrescherleistung um ca. 15 bis 20 Prozent steigern. Diese Beispiele unterstreichen zugleich die Forderung, dass die Druscheignung nicht erst zur Ernte diese Führungsrolle übernimmt, sondern auch in den vorgelagerten Verfahrensabschnitten als ein starkes Entscheidungskriterium gelten muss. / The parameter “threshability” of a stand is commonly assumed to be a criterion defined by the genetics of the strain and the weather conditions which is mainly not influenced by the farmer. Nevertheless, the farmer continuously changes the “threshability” with all his decisions, from the selection of the strains, via the use of fertilizers to the harvest management. For harvest, as the last stage of the process chain, the threshability develops extraordinarily high economic effects. On the basis of several examples from cultivation, plant nutrition, plant protection and harvest technology it was described, how the threshability can be influenced and how this effect is to be assessed monetarily. In the field of cultivation, the development of a new growth type of semi-dwarf rape has been selected, which yields competitive returns with less bio mass. Ripeness is more homogeneous, the harvest date can be fixed without problems, threshing is efficient and with low loss. Using the method of siccation, late and intensively controlled strains can be harvested earlier and easier. This offers harvest safety with low loss, higher combine harvester performance and reduced fuel consumption.The need-based spreading of nitrogen referring to type, quantity and time according to the small-scale spatial heterogeneity of a field leads to a homogenisation of the stands. The stands ripen more evenly and this fact causes about 20 percent higher combine harvester performance, as well as reduced fuel consumption. Using the example of high-cut top harvest it was clarified how the better harvestability influences the machine costs, the total harvest losses, the quality, and the drying process. If the length of the stubbles is extended by 10 centimetres, the combine harvester performance can be increased by 15 to 20 percent. These examples also emphasize the requirement that the threshability does not just take over the leading role for the harvest but has also to be considered as a strong decision criterion within the prior stages of the process.
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