Trophic cascades involving large terrestrial mammals are very seldom empirically demonstrated. The high species diversity often associated with terrestrial systems is thought to modulate the strength of trophic cascades. In speciose systems, species often vary in vulnerability to consumption, hence some species are less responsive to top-down pressure. African large mammalian herbivores are highly diverse and vary greatly in body size, a characteristic linked to vulnerability to predation. Moreover, Africa is one of the last places to still support megaherbivores, species that have grown large enough to be practically impervious to non-human predation. Thus an African ecosystem is the ideal setting to explore trophic cascades in speciose terrestrial systems. In this study I explored patterns in trophic interaction amongst carnivores, vulnerable mesoherbivore and predator-invulnerable megaherbivores in an intact African savanna at a variety of spatial scales. I examined the mechanistic links between trophic levels at the patch scale through fine scale empirical manipulation of predation risk. In addition, I assessed the landscape scale biological relevance of these trophic interactions through correlative observations over large spatiotemporal scales. I present the first evidence of community level trophic cascades explicitly involving megaherbivores. I demonstrate how megaherbivores’ disregard of predation risk masks the effects of predator-triggered trophic cascades and weakens their impact on the ecosystem. The risk of predation triggered a spatial response in vulnerable species, driving them into safe areas, yet did not influence the space use of megaherbivores. This species-specific spatial response had contrasting effects on nutrient distribution. Vulnerable herbivores’ fear-induced foraging behaviour led to localised nutrient accumulation whereas the foraging behaviour of predator-invulnerable megaherbivores led to nutrient redistribution across the landscape. In addition, the fear-driven spatial differences in mesoherbivore grazing impact and nutrient deposition led to landscape scale changes in the distribution and persistence of herbivore maintained grass communities, so-called grazing lawns. However, the grazing activity of the in-vulnerable megagrazer, white rhino (Ceratotherium simum) weakened the effect of this trophic cascade by creating and maintaining grazing lawns within the risky habitat avoided by mesograzers. This study contributes to our understanding of what drives the variation among patterns of trophic control and provides the first evidence of the modulating influence that megaherbivores have on predator-triggered trophic cascades.

Freshwater ecosystem degradation in the Amazon and Orinoco river basins is increasing due to rising human population numbers, and large water development projects. Therefore, monitoring ecosystem condition in these rivers of high biodiversity is of global conservation importance. This dissertation evaluated the potential of using river dolphins as indicators of ecosystem condition in large tropical rivers of South America. First, population estimates of river dolphins were obtained by line-strip transect surveys and mark-recapture methods on photo-identifications. Using this information, I identified critical habitat, hotspots, and areas of concern for river dolphins, as well as the relationship between dolphin density and group size estimates with ecological features. Second, I evaluated the relationship between dolphin estimates and human stressors. Higher densities of dolphins occurred in rivers with low indices of overall freshwater degradation, such as rivers with high water quality and the lowest human population numbers. Thus, dolphin density estimates seem to be good indicators of freshwater ecosystem degradation in the Amazon and Orinoco basins. These top predators not only are indicator species, but also have the potential to act as flagship and sentinel species, indicating freshwater ecosystem degradation and stimulating conservation action. This dissertation highlights the large changes in the Amazon and Orinoco that are approaching fast. Indicator, flagship and sentinel species can become science-based conservation tools not to only document freshwater ecosystem degradation, but to raise awareness about broader implications of human stressors on biodiversity and river systems.

Mid-latitude shelf-seas are highly productive regions that host a rich diversity of animals including large numbers of marine mammals and seabirds. These large vertebrate predators play a crucial role in the functioning of shelf-sea ecosystems. However, the combined effects of multiple anthropogenic stressors are driving unprecedented declines in many of their populations. Mitigating this depends upon effective conservation and integrated ecosystem based approaches to management, which require a comprehensive understanding of the habitat needs of marine predators. The foraging efficiencies of marine predators are closely tied to the availability of a number of oceanographic features. As such, these physical habitats represent critical locations within a species’ range whose preservation and protection should be of high priority. The collection of studies presented in this thesis aims to improve our understanding of the physical oceanographic processes that underlie the at-sea behaviours and distributions of marine mammals and seabirds in coastal and shelf-sea environments. A combination of at-sea boat surveying, animal-borne biologging, satellite remote-sensing, passive acoustics and numerical modelling was used to collect information on the distributions and foraging behaviours of a range of marine predators alongside the bio-physical characteristics of the oceanographic habitats they occupied. These data were then used to (1) examine the use of oceanographic habitats generated around tidal-mixing fronts and coastal topographic structures by a range of piscivorous species including bottlenose dolphins Tursiops truncatus, common dolphins Delphinus delphis, harbour porpoises Phocoena phocoena and northern gannets Morus bassanus, and (2) identify the physical processes underlying their creation. Original aspects of this work include the examination of the fine-scale bio-physical mechanisms that link marine predators to tidal-mixing fronts and coastal tidal-topographic structures. Main findings indicate that offshore habitats around tidal-mixing fronts are used by both common dolphins and northern gannets for foraging. Individuals associated with patches of increased sub-surface primary productivity, which were generated via a bi-weekly cycle of episodic turbulent mixing and stratification following an adjustment in the spatial position of a front with the spring-neap cycle. Moreover, around fronts, the dives of gannets were likely to be short and of a V-shaped strategy (with little active swim phase), which likely reflects an increase in the accessibility and catchability of their prey. In a coastal estuarine system, bottlenose dolphins were shown to associate with predictable downwelling features generated during flood tidal flows that were thought to act as a foraging aid. Together, these findings highlight the fundamental role physical oceanographic processes play in the structuring of marine ecosystems by providing vulnerable marine predators with prosperous and reliable foraging resources that they can exploit. This work has implications for both future studies of marine predator foraging ecology and the management of anthropogenic activities in coastal and shelf-seas.

As aves de rapina, como predadores de níveis superiores na rede trófica, necessitam de grandes áreas de vida e ocorrem em baixa densidade populacional. São sensíveis a alteração e degradação do habitat, sendo bons indicadores de qualidade de habitat e biodiversidade. Apesar da importância ecológica pouco se conhece sobre a biologia e ecologia da maioria das 91 espécies que ocorrem na América do Sul, o que nos leva ao objetivo mais amplo desta tese: descrever a composição das taxocenoses de aves de rapina dos campos do sul do Brasil e Uruguai e sua relação com as características topográficas e ambientais. Para tanto, foram realizadas amostragens de aves de rapina em 11 áreas campestres entre outono de 2009 e verão de 2011. Um total de 44 transectos rodoviários foi amostrado sazonalmente, duas vezes ao ano, totalizando 176 amostragens. Ao todo foram observadas 18.424 aves de rapina, pertencentes a 33 espécies. Os transectos rodoviários são amplamente utilizados para a amostragem de aves de rapina, no entanto, nossos resultados sugerem que este método pode subestimar a abundância das espécies. Pontos de contagem parecem ser mais eficazes, principalmente para a amostragem de espécies raras. A distribuição das espécies foi associada à altitude e disponibilidade de habitat, e quatro taxocenoses puderam ser identificadas no sudeste da América do Sul. A taxocenose dos campos de altitude (na ecorregião Floresta Úmida com Araucária) se caracterizou pela presença de espécies florestais, ausentes ou pouco abundantes nas demais áreas. Na ecorregião Savana Uruguaia foram distinguidas outras três taxocenoses: 1) campos da planície costeira do sul do Brasil, com espécies associadas às áreas úmidas e ambientes alterados; 2) campos da Serra do Sudeste, uma taxocenose mista com espécies campestres e florestais, porém pouco abundantes e com maior tolerância a habitat alterados; 3) campos da Savana Uruguaia, taxocenose com predominância de espécies campestres e presença pontual de espécies florestais associadas a matas riparias. Foram registradas quatro espécies de necrófagos obrigatórios (Cathartiformes). A organização da taxocenose dos necrófagos aparenta ser mais influenciada pelas características da paisagem que por relações hierárquicas interespecíficas, mas a distribuição atual pode ter sido influenciada pela caça e envenenamento das espécies, ocorrida no século passado. A abundância das aves de rapina variou sazonalmente. A abundância foi significativamente maior no verão que no inverno, mas variou conforme a espécie. Quatro espécies são migratórias (duas migrantes trans-equatorias e duas intratropicais), três são parcialmente migratórias, mas o padrão de migração e as rotas são pouco conhecidos, e três espécies são nômades ou realizam movimentos irruptivos. Os campos do sudeste da América do Sul tem uma alta diversidade de aves de rapina, influenciada pela topografia, disponibilidade de habitat e sazonalidade, características que devem ser consideradas nas políticas de conservação na região. / Raptors, as top predators, have large home ranges and low population densities. They are sensitive to human perturbation and habitat degradation; hence raptors are sentinel species and biodiversity indicators. Despite their ecological importance, the biology and ecology of most of the 91 South American raptors is poorly known. The main objectives of this study were to examine the assemblage composition in grasslands of southern Brazil and Uruguay and to investigate its relation with topography and habitat features. To achieve this, 44 roadside transects were conducted in 11 areas. Each transect was survey four times between autumn/2009 and summer/2011. The 176 transects yielded 18,424 contacts of raptors, belonging to 34 species. Roadside line transect is widely used to survey raptors. However, our results suggest that this method could underestimate the species abundance. Roadside point transect seems to be better, especially to survey rare species. Raptors distribution and assemblage composition were related to altitude and habitat availability, and four assemblages could be identified in southeastern South America. Raptor assemblage at Altitudinal grasslands (in the Araucarian Moist Forest ecoregion) had higher abundances of forest raptors than the other regions. Three assemblages were distinguished in the Uruguayan Savannas ecoregion: 1) south Brazilian coastal plain, with species related wetlands and human modified habitats; 2) Serra do Sudeste grasslands, a mix of forest and grassland raptors assemblage, which showed low abundances and lower sensibility to habitat degradation; 3) Uruguayan Savannas, dominated by grasslands species and few forest species usually found in riparian habitats. Four obligate scavengers (Cathartiformes) were recorded. Landscape features appeared to be more important to the assemblage organization than interspecific hierarchy, but the current distribution and abundance of scavengers could have been driven by species persecution and poisoning in the last century. Raptors abundance changed seasonally. Total abundance was significantly higher during summer than winter, and strategies to deal with seasonality varied between species. Four species are complete migrants (two transequatorial and two intratropical), three are partial migrants, but their routes and migration pattern remain poorly understood; and three species are irruptive or nomadic. The grasslands of southern South America have a high diversity of raptors, influenced by topography, habitat availability and seasonality, features that should be taken into account when divising regional conservation policies.

As aves de rapina, como predadores de níveis superiores na rede trófica, necessitam de grandes áreas de vida e ocorrem em baixa densidade populacional. São sensíveis a alteração e degradação do habitat, sendo bons indicadores de qualidade de habitat e biodiversidade. Apesar da importância ecológica pouco se conhece sobre a biologia e ecologia da maioria das 91 espécies que ocorrem na América do Sul, o que nos leva ao objetivo mais amplo desta tese: descrever a composição das taxocenoses de aves de rapina dos campos do sul do Brasil e Uruguai e sua relação com as características topográficas e ambientais. Para tanto, foram realizadas amostragens de aves de rapina em 11 áreas campestres entre outono de 2009 e verão de 2011. Um total de 44 transectos rodoviários foi amostrado sazonalmente, duas vezes ao ano, totalizando 176 amostragens. Ao todo foram observadas 18.424 aves de rapina, pertencentes a 33 espécies. Os transectos rodoviários são amplamente utilizados para a amostragem de aves de rapina, no entanto, nossos resultados sugerem que este método pode subestimar a abundância das espécies. Pontos de contagem parecem ser mais eficazes, principalmente para a amostragem de espécies raras. A distribuição das espécies foi associada à altitude e disponibilidade de habitat, e quatro taxocenoses puderam ser identificadas no sudeste da América do Sul. A taxocenose dos campos de altitude (na ecorregião Floresta Úmida com Araucária) se caracterizou pela presença de espécies florestais, ausentes ou pouco abundantes nas demais áreas. Na ecorregião Savana Uruguaia foram distinguidas outras três taxocenoses: 1) campos da planície costeira do sul do Brasil, com espécies associadas às áreas úmidas e ambientes alterados; 2) campos da Serra do Sudeste, uma taxocenose mista com espécies campestres e florestais, porém pouco abundantes e com maior tolerância a habitat alterados; 3) campos da Savana Uruguaia, taxocenose com predominância de espécies campestres e presença pontual de espécies florestais associadas a matas riparias. Foram registradas quatro espécies de necrófagos obrigatórios (Cathartiformes). A organização da taxocenose dos necrófagos aparenta ser mais influenciada pelas características da paisagem que por relações hierárquicas interespecíficas, mas a distribuição atual pode ter sido influenciada pela caça e envenenamento das espécies, ocorrida no século passado. A abundância das aves de rapina variou sazonalmente. A abundância foi significativamente maior no verão que no inverno, mas variou conforme a espécie. Quatro espécies são migratórias (duas migrantes trans-equatorias e duas intratropicais), três são parcialmente migratórias, mas o padrão de migração e as rotas são pouco conhecidos, e três espécies são nômades ou realizam movimentos irruptivos. Os campos do sudeste da América do Sul tem uma alta diversidade de aves de rapina, influenciada pela topografia, disponibilidade de habitat e sazonalidade, características que devem ser consideradas nas políticas de conservação na região. / Raptors, as top predators, have large home ranges and low population densities. They are sensitive to human perturbation and habitat degradation; hence raptors are sentinel species and biodiversity indicators. Despite their ecological importance, the biology and ecology of most of the 91 South American raptors is poorly known. The main objectives of this study were to examine the assemblage composition in grasslands of southern Brazil and Uruguay and to investigate its relation with topography and habitat features. To achieve this, 44 roadside transects were conducted in 11 areas. Each transect was survey four times between autumn/2009 and summer/2011. The 176 transects yielded 18,424 contacts of raptors, belonging to 34 species. Roadside line transect is widely used to survey raptors. However, our results suggest that this method could underestimate the species abundance. Roadside point transect seems to be better, especially to survey rare species. Raptors distribution and assemblage composition were related to altitude and habitat availability, and four assemblages could be identified in southeastern South America. Raptor assemblage at Altitudinal grasslands (in the Araucarian Moist Forest ecoregion) had higher abundances of forest raptors than the other regions. Three assemblages were distinguished in the Uruguayan Savannas ecoregion: 1) south Brazilian coastal plain, with species related wetlands and human modified habitats; 2) Serra do Sudeste grasslands, a mix of forest and grassland raptors assemblage, which showed low abundances and lower sensibility to habitat degradation; 3) Uruguayan Savannas, dominated by grasslands species and few forest species usually found in riparian habitats. Four obligate scavengers (Cathartiformes) were recorded. Landscape features appeared to be more important to the assemblage organization than interspecific hierarchy, but the current distribution and abundance of scavengers could have been driven by species persecution and poisoning in the last century. Raptors abundance changed seasonally. Total abundance was significantly higher during summer than winter, and strategies to deal with seasonality varied between species. Four species are complete migrants (two transequatorial and two intratropical), three are partial migrants, but their routes and migration pattern remain poorly understood; and three species are irruptive or nomadic. The grasslands of southern South America have a high diversity of raptors, influenced by topography, habitat availability and seasonality, features that should be taken into account when divising regional conservation policies.

As aves de rapina, como predadores de níveis superiores na rede trófica, necessitam de grandes áreas de vida e ocorrem em baixa densidade populacional. São sensíveis a alteração e degradação do habitat, sendo bons indicadores de qualidade de habitat e biodiversidade. Apesar da importância ecológica pouco se conhece sobre a biologia e ecologia da maioria das 91 espécies que ocorrem na América do Sul, o que nos leva ao objetivo mais amplo desta tese: descrever a composição das taxocenoses de aves de rapina dos campos do sul do Brasil e Uruguai e sua relação com as características topográficas e ambientais. Para tanto, foram realizadas amostragens de aves de rapina em 11 áreas campestres entre outono de 2009 e verão de 2011. Um total de 44 transectos rodoviários foi amostrado sazonalmente, duas vezes ao ano, totalizando 176 amostragens. Ao todo foram observadas 18.424 aves de rapina, pertencentes a 33 espécies. Os transectos rodoviários são amplamente utilizados para a amostragem de aves de rapina, no entanto, nossos resultados sugerem que este método pode subestimar a abundância das espécies. Pontos de contagem parecem ser mais eficazes, principalmente para a amostragem de espécies raras. A distribuição das espécies foi associada à altitude e disponibilidade de habitat, e quatro taxocenoses puderam ser identificadas no sudeste da América do Sul. A taxocenose dos campos de altitude (na ecorregião Floresta Úmida com Araucária) se caracterizou pela presença de espécies florestais, ausentes ou pouco abundantes nas demais áreas. Na ecorregião Savana Uruguaia foram distinguidas outras três taxocenoses: 1) campos da planície costeira do sul do Brasil, com espécies associadas às áreas úmidas e ambientes alterados; 2) campos da Serra do Sudeste, uma taxocenose mista com espécies campestres e florestais, porém pouco abundantes e com maior tolerância a habitat alterados; 3) campos da Savana Uruguaia, taxocenose com predominância de espécies campestres e presença pontual de espécies florestais associadas a matas riparias. Foram registradas quatro espécies de necrófagos obrigatórios (Cathartiformes). A organização da taxocenose dos necrófagos aparenta ser mais influenciada pelas características da paisagem que por relações hierárquicas interespecíficas, mas a distribuição atual pode ter sido influenciada pela caça e envenenamento das espécies, ocorrida no século passado. A abundância das aves de rapina variou sazonalmente. A abundância foi significativamente maior no verão que no inverno, mas variou conforme a espécie. Quatro espécies são migratórias (duas migrantes trans-equatorias e duas intratropicais), três são parcialmente migratórias, mas o padrão de migração e as rotas são pouco conhecidos, e três espécies são nômades ou realizam movimentos irruptivos. Os campos do sudeste da América do Sul tem uma alta diversidade de aves de rapina, influenciada pela topografia, disponibilidade de habitat e sazonalidade, características que devem ser consideradas nas políticas de conservação na região. / Raptors, as top predators, have large home ranges and low population densities. They are sensitive to human perturbation and habitat degradation; hence raptors are sentinel species and biodiversity indicators. Despite their ecological importance, the biology and ecology of most of the 91 South American raptors is poorly known. The main objectives of this study were to examine the assemblage composition in grasslands of southern Brazil and Uruguay and to investigate its relation with topography and habitat features. To achieve this, 44 roadside transects were conducted in 11 areas. Each transect was survey four times between autumn/2009 and summer/2011. The 176 transects yielded 18,424 contacts of raptors, belonging to 34 species. Roadside line transect is widely used to survey raptors. However, our results suggest that this method could underestimate the species abundance. Roadside point transect seems to be better, especially to survey rare species. Raptors distribution and assemblage composition were related to altitude and habitat availability, and four assemblages could be identified in southeastern South America. Raptor assemblage at Altitudinal grasslands (in the Araucarian Moist Forest ecoregion) had higher abundances of forest raptors than the other regions. Three assemblages were distinguished in the Uruguayan Savannas ecoregion: 1) south Brazilian coastal plain, with species related wetlands and human modified habitats; 2) Serra do Sudeste grasslands, a mix of forest and grassland raptors assemblage, which showed low abundances and lower sensibility to habitat degradation; 3) Uruguayan Savannas, dominated by grasslands species and few forest species usually found in riparian habitats. Four obligate scavengers (Cathartiformes) were recorded. Landscape features appeared to be more important to the assemblage organization than interspecific hierarchy, but the current distribution and abundance of scavengers could have been driven by species persecution and poisoning in the last century. Raptors abundance changed seasonally. Total abundance was significantly higher during summer than winter, and strategies to deal with seasonality varied between species. Four species are complete migrants (two transequatorial and two intratropical), three are partial migrants, but their routes and migration pattern remain poorly understood; and three species are irruptive or nomadic. The grasslands of southern South America have a high diversity of raptors, influenced by topography, habitat availability and seasonality, features that should be taken into account when divising regional conservation policies.

Variable ocean conditions can greatly impact lower trophic level prey assemblages in marine ecosystems, with effects propagating up to higher trophic levels. Our goal was to better understand how varying ocean conditions influence diets and niche overlap among a suite of low- to mid trophic level predators. We studied the diets of common murres (Uria aalge) over 10 contrasting years between 1998 and 2011, a period in which the Northern California Current experienced dramatic interannual variability in ocean conditions. Likewise, murre diets off Oregon varied considerably. Interannual variation in murre chick diets appears to be influenced by environmental drivers occurring before and during the breeding season, at both basin and local spatial scales. While clupeids were an important diet component throughout the study period, in some years murre diets were dominated by Pacific sand lance (Ammodytes hexapterus) and other years by osmerids (likely Allosmerus elongatus and Hypomesus pretiosus). Years in which the Pacific Decadal Oscillation and local sea surface temperatures were above average during summer months also showed elevated levels of clupeids in murre diets, while years with higher winter ichthyoplankton biomass and summer northern copepod biomass anomalies had fewer clupeids and more sand lance and smelts. Years with higher Northern Oscillation Index values during summer months also showed more smelts in the murre diets. Nesting phenology and reproductive success were correlated with diet as well, reflecting demographic consequences of environmental variability mediated through bottom-up food web dynamics. To examine niche overlap between murres and other marine predators we employed collaborative fisheries research with synoptic observations of a major seabird colony to determine the diets of four predator species on the central Oregon coast during two years of contrasting El Niño (2010) vs. La Niña (2011) conditions. The greatest degree of dietary overlap was observed between Chinook salmon (Oncorhynchus tshawytscha) and common murres, with both smelts (Osmeridae) and clupeids (primarily Clupea pallasii) observed as the dominant prey types. Diets differed between El Niño and La Niña conditions for two predators, murres and black rockfish (Sebastes melanops). During La Niña, smelts decreased, while sand lance increased in common murre diets. Black rockfish had fewer larval Dungeness crabs (Cancer magister) and a greater proportion of crab species associated with the later spring transition. Chinook salmon and Pacific halibut (Hippoglossus stenolepis) diets were similar during El Niño and La Niña conditions. These findings underscore that the diets of common murres during chick rearing reflect local- and basin-scale biophysical processes in the Northern California Current, and are valuable for understanding the response of upper trophic level organisms to changing oceanographic conditions. Additionally, using multiple predators across several diverse taxa to track changes in prey communities provided a way to detect seemingly subtle changes in prey communities and contributes to a more comprehensive understanding of food web dynamics and ecosystem indicators. / Graduation date: 2012

Les nombreuses menaces qui pèsent sur le milieu marin et les preuves d’une perte de biodiversité globale au cours des dernières décennies ont rendu indispensable la mise en place de mesures de conservation dans les années à venir. Au sein des Terres Australes et Antarctiques Françaises (TAAFs) en particulier, qui offrent une biodiversité exceptionnelle et sont le cadre d’activités anthropiques intensives à travers les grandes pêcheries industrielles, la délimitation d’Aires Marines Protégées (AMP) serait extrêmement bénéfique pour la conservation du milieu. Pour définir les zones d’importance pour la biodiversité, qui représenteraient de bonnes candidates pour un statut d’AMP, l’utilisation des données de distribution de prédateurs supérieurs apparait idéale. En effet, leur position dans le réseau trophique en fait de bons intégrateurs des niveaux inférieurs, et de plus, ils sont facilement observables lors des campagnes d’observations et offrent des facilités pour l’équipement de dispositifs télémétriques puisqu’ils reviennent régulièrement à terre pendant la période de reproduction. Grâce à l’analyse spatiale des données de distribution obtenues pour un grand nombre de prédateurs supérieurs au sein d’écosystèmes subantarctiques aussi bien qu’en milieu tropical et grâce au développement de modèles d’habitat, nous avons ici déterminé des ‘hotspots’ de biodiversité pour les prédateurs supérieurs des TAAFs. Ces travaux pourraient ainsi servir de base aux propositions de limites pour de potentielles futures AMPs. / The numerous threats that marine environment face, coupled with the evidence for a global biodiversity loss during last decades have lead to an increasing need for setting up conservation measures. Particularly, delimiting Marine Protected Areas (MPAs) within the French Southern Territories, which are home to an exceptional biodiversity and extensive human activities through industrial fisheries, would be extremely beneficial for species conservation. In order to identify areas of ecological significance for biodiversity, which could be good MPA candidates, the use of distribution data from top marine predators seems to be ideal. Thanks to their high position in trophic network, they are likely to integrate lowers trophic level species distribution. Moreover, they are easy to observe trough at-sea observation campaigns, and easy to equip with telemetric devices thank to their central place foraging that lead them to return regularly to their colony during breeding season. Here, we analyse distribution data and develop habitat models from several top predators species in both subantarctic and tropical regions to highlight biodiversity hotspots within the French Southern Territories. This work could thus be use as a basis to define potential boundaries for a future MPA.

Les tendances spatiales et temporelles du mercure en Arctique ont été étudiées au travers de l’analyse de ce métal dans les tissus durs, i.e. dents et poils, des phoques annelés (Phoca hispida) et des ours polaires (Ursus maritimus). Aucune influence du sexe sur les concentrations n’a été détectée dans les tissus de ces deux espèces alors que l’âge est apparu comme un facteur d’influence important.Deux tendances spatiales ont été observées dans les tissus de ces deux espèces : un premier gradient d’augmentation du mercure de l’Est vers l’Ouest de l’Arctique, i.e. de Svalbard, vers le Groenland et enfin, l’Arctique canadien, et un second du Sud vers le Nord de l’Arctique canadien, résultant très probablement de la minéralogie du socle rocheux, mais aussi potentiellement de facteurs biotiques et abiotiques.Une tendance à l’augmentation des concentrations de mercure a été globalement détectée entre la période préindustrielle et la fin du XXe siècle. Cependant, les variations temporelles associées à la seconde partie du XXe siècle révèlent une augmentation continue dans l’Ouest de l’Arctique et une tendance à la diminution dans l’Est de l’Arctique, cette différence étant probablement liée à des apports distincts des masses d’air atmosphériques à ces deux régions. Par ailleurs, une composante de variabilité climatique pourrait contribuer aux variations observées ces dernières décennies en influençant les habitudes alimentaires de ces prédateurs marins arctiques. Ainsi, les isotopes stables d’azote et de carbone s’avèrent être un outil essentiel à coupler aux études sur les tendances de mercure pour permettre de déterminer la part d’un changement dans le régime alimentaire ou dans les niveaux environnementaux.Cette étude a mis en évidence l’importance des poils comme tissu de monitoring non-invasif et pertinent pour un suivi régulier voire annuel tandis que l’utilisation des dents s’avère être plus adaptée à la reconstruction de tendances sur le long-terme. / The spatial and temporal trends of mercury in the Arctic have been studied through the analysis of this metal in hard tissues, i.e. teeth and hair, in ringed seals (Phoca hispida) and polar bears (Ursus maritimus). Sex had no influence on the mercury concentrations while age was a significant factor. Two distinct spatial trends were observed in the tissues of these two species: a first gradient of augmentation from the East to the West of the Arctic, i.e. from Svalbard towards Greenland and the Canadian Arctic, and a second one, from the South to the North of the Canadian Arctic, resulting probably from a combination of the global mineralogy in the Arctic with biotic and abiotic factors. An increasing trend in mercury concentrations has been detected globally between the preindustrial period and the end of the 20th century. However, the temporal trends detected in the second part of the 20th century revealed an increase in the West Arctic while a decrease was observed in the East Arctic. This difference seems to be due to a distinct input of the atmospheric air masses to the two regions. Besides, a climatic variability could contribute to the variations observed these last decennials by influencing the habitat and the feeding habits of the marine predators. Thus, the determination of the stable isotopes seems to be essential to combine to the study of the mercury trends. Finally, this study has showed the importance of hair as a non-invasive and relevant biomonitoring tissue on a regular or annual sampling base, while the use of the teeth seems to be more adequate for reconstructing long-term trends of mercury.

There is a widespread distribution of possibly toxic heavy metals, such as zinc, in aquatic ecosystems. Simultaneously aquatic food webs are changing due to declining predatory fish stocks. In this study, I examine how such an alteration of the food chain, the disappearance of a top trophic level, can affect the uptake and transfer of pollutants in lower trophic levels. I investigate a tri-trophic food chain containing resources (zooplankton), consumer fish, and predatory fish and use zinc as an example pollutant. This is done by constructing and adding a pollutant module to a stage-structured biomass model. The combined model is based on a system of eight ordinary differential equations to study the zinc concentrations in the consumer fish population in two scenarios: in the presence or absence of predatory fish, i.e., the food chain consists of either three or two trophic levels. The results show that the removal of the predator affects the concentration of the pollutant in the consumer population. In the absence of predators, the uptake of zinc from food is smaller and the zinc concentration is lower in the consumer fish population. The results remain the same for different values of the pollutant-specific parameters, i.e., uptake rate from water, assimilation efficiency, and efflux rate. This could indicate that food chain dynamics have a strong influence on the uptake and transfer of pollutants. Furthermore, this means that even if the model is parametrized to zinc in this study, the modeling tool can also be used for other pollutants with similar biokinetics as zinc. The results from this study highlight the importance to include food chain structure and dynamics when studying the uptake and transfer of pollutants. The novel knowledge and the developed tool from this study could advantageously be included to a higher degree when discussing the impact of pollution on aquatic ecosystems and mitigation measures. / Det finns en utbredd spridning av potentiellt giftiga tungmetaller, som t.ex. zink, i akvatiska ekosystem. Samtidigt förändras akvatiska födovävar på grund av minskande rovfiskbestånd. I denna studie undersöker jag hur en sådan förändring av födoväven, ett försvinnande av en trofisk toppnivå, kan påverka upptaget och överföringen av föroreningari lägre trofinivåer. Jag undersöker en tritrofisk födokedja som innehåller resurser (zooplankton), konsumentfisk och rovfisk och använder zink som ett exempel på förorening. Detta görs genom att konstruera och addera en föroreningsmodul till en stegstrukturerad biomassamodell. Den kombinerade modellen bygger på ett system med åtta ordinära differentialekvationer för att studera zinkhalterna i konsumentfiskpopulationen i två scenarier: i närvaro eller frånvaro av predatorer, dvs. födokedjan innehåller antingen tre eller två trofiska nivåer. Resultaten visar att koncentrationen av föroreningen i konsumentpopulationen förändras när födokedjan ändras. När rovfiskar saknas i systemet är upptaget av zink från föda mindre och zinkkoncentrationen lägre i konsumentfiskpopulationen. Resultaten förblir detsamma för olika värden på de föroreningsspecifika parametrarna, d.v.s. upptagshastighet från vatten, assimileringseffektivitet och utflödeshastighet, vilket skulle kunna indikera att födokedjans dynamik har ett starkt inflytande på upptag och överföring av föroreningar. Vidare innebär detta att även om modellen har parametriserats till zink i denna studie, så kan modelleringsverktyget även användas för andra föroreningar som har en liknande biokinetik som zink. Resultaten från denna studie understryker vikten av att inkludera födokedjans struktur och dynamik när man studerar upptag och överföring av föroreningar. Den nya kunskapen och det utvecklade verktyget från denna studie skulle med fördel i högre grad inkluderas när man diskuterar föroreningars påverkan på akvatiska ekosystem och vilka åtgärder som bör sättas in för att minska problemen.