published_or_final_version / Physics / Master / Master of Philosophy

Zinc oxide (ZnO), as a Ⅱ-Ⅵ compound semiconductor with a wide direct band gap, has attracted great attention from the worldwide researchers for its potential application in the fields of spintronics and optoelectronics. At present research about the defects in ZnO and ZnO-based materials is still far from complete. The deep level defects in melted grown ZnO single crystal induced by helium ions implantation and electron irradiation, as well as their thermal evolution, were studied in this research using the technique of deep level transient spectroscopy (DLTS) and photoluminescence (PL). DLTS results indicated that, besides E3 (????~0.28 ????) trap which was widely observed in the as-grown ZnO samples, the deep level with ????~0.92 ???? was also indentified in the helium-implanted ZnO samples, which was introduced by the ion implantation and tentatively assigned to be the oxygen vacancy (VO). This deep level was removed after 350 oC annealing in argon gas. Annealing at 350 oC also brought along a new deep level with ????~0.66???? into helium-implanted samples which could be annealed out by 650 oC annealing in argon gas. The electron irradiation induced a deep level with ????~0.59 ???? into ZnO, which was probably associated with the singly charged state of VO. This deep level also tended to be removed at 350 oC annealing in argon gas. The PL spectra revealed that both helium implantation and electron irradiation could improve the bound-exciton peak. Helium implantation also introduced defects emission at 1.90 eV , which was the red luminescence band, into the ZnO single crystal materials. This red luminescence band peak might be associated with DAP recombination. Electron irradiation might restrain the green luminescence in ZnO single crystal. The fine structures could disappear as the measurement temperature increased, leaving the green luminescence band only. / published_or_final_version / Physics / Doctoral / Doctor of Philosophy

published_or_final_version / Physics / Master / Master of Philosophy

Background/Objectives: Gaps in care due to the movement of patients between health settings and/or practitioners, known as transitions of care (TOC), may contribute to second stroke or TIA events. The elements that impact TOC in the stroke/TIA population have not been fully elucidated. The purpose of this study is to identify key elements of a Doctor of Nursing Practice-led TOC model that could be used to develop and evaluate a TOC program for the stroke/TIA population. Design: A descriptive study was performed to 1) identify elements that may affect transitions of care using a stroke database and post-discharge phone surveys and 2) based on information from Aim 1, propose a DNP-led TOC model specific to the stroke/TIA. Setting: An urban primary stroke center in the southwest United States. Participants: All patients in the GWTG®-stroke database from May 1 - December 31st, 2012 and patients who consented at discharge from the stroke unit following a stroke or TIA. Measurements: Patient demographics including: length of stay (LOS), age, race, ethnicity, comorbidities, insurance, discharge status, thirty-day readmission rate, and follow up survey. Results: Patient data (n=276) from GWTG®-stroke database was obtained. Average LOS was 7.81 +/- 11.15 days. The majority of patients were greater than age 65 (59%); 53% relied on Medicare support; those age 50-59 (21%) were most likely to be uninsured (47%). Fifty-one percent were discharged directly home, 48% of those were referred to outpatient rehab services. Two-thirds received rehabilitation services during hospitalization. Eight patients experienced a subsequent hospital readmission; two of those had a repeat stroke event. Although patients reported understanding their discharge instructions, their perception of ongoing care was poor. Conclusion: Key elements of a TOC model specific to the stroke and TIA patient population could include: patient surveillance, comprehensive care planning, follow-up, stroke education and point of contact. Advanced practice nurses have been successful in leading such programs, and a DNP-led model providing continuity of care would support the transition of an effective model into clinical practice.

An experimental investigation to study the influence of base metal composition on the time required to achieve complete isothermal solidification (tf) during TLP bonding of three Ni-base superalloys was performed. Alloys IN 738, DS Rene80 and DS IC 6 show similar behaviour when bonded at 1100 oC, with comparable tf. However, at higher temperatures, IN 738 requires extended period of time (as compared to DS Rene80 and DS IC 6) to achieve complete isothermal solidification. The prolonged tf in IN 738 appears to be caused by a more pronounced reduction in concentration gradient of the diffusing solute within the material during bonding. In contrast, the shorter complete isothermal solidification time experienced by alloy DS IC6 is attributable to its capability to better accommodate the diffusing solute, through the formation of densely packed second-phase precipitates in the diffusion affected zone (DAZ).

The SLC30A2 zinc transporter has been investigated due to its important role in zinc secretion into human milk. SLC30A2 is expressed in mammary epithelial cells, and the presence of genetic variations in this transporter could cause low zinc transport into the milk, leading to Transient Neonatal Zinc Deficiency (TNZD) in newborns. Through bioinformatics analysis 22 SNPs were identified. Therefore, we aim to identify the functional changes caused by 4 SNPs. By subcloning the SLC30A2 open reading frames into the Gateway expression plasmid tagged with red and green fluorescent proteins (mCherry, tGFP). Four SNPs were introduced by mutagenesis and tagged with mCherry. We transfected individual plasmids into mammary epithelial cells (HC11) and observed cellular targeting using epifluorescent imaging. The most common variants located to secreting endosomes and membrane in HC11 cells. Incorrect targeting of SLC30A2 causes mislocalization. It may be possible to identify mothers carrying risk genotypes for infant zinc deficiency.

An experimental investigation to study the influence of base metal composition on the time required to achieve complete isothermal solidification (tf) during TLP bonding of three Ni-base superalloys was performed. Alloys IN 738, DS Rene80 and DS IC 6 show similar behaviour when bonded at 1100 oC, with comparable tf. However, at higher temperatures, IN 738 requires extended period of time (as compared to DS Rene80 and DS IC 6) to achieve complete isothermal solidification. The prolonged tf in IN 738 appears to be caused by a more pronounced reduction in concentration gradient of the diffusing solute within the material during bonding. In contrast, the shorter complete isothermal solidification time experienced by alloy DS IC6 is attributable to its capability to better accommodate the diffusing solute, through the formation of densely packed second-phase precipitates in the diffusion affected zone (DAZ).

The SLC30A2 zinc transporter has been investigated due to its important role in zinc secretion into human milk. SLC30A2 is expressed in mammary epithelial cells, and the presence of genetic variations in this transporter could cause low zinc transport into the milk, leading to Transient Neonatal Zinc Deficiency (TNZD) in newborns. Through bioinformatics analysis 22 SNPs were identified. Therefore, we aim to identify the functional changes caused by 4 SNPs. By subcloning the SLC30A2 open reading frames into the Gateway expression plasmid tagged with red and green fluorescent proteins (mCherry, tGFP). Four SNPs were introduced by mutagenesis and tagged with mCherry. We transfected individual plasmids into mammary epithelial cells (HC11) and observed cellular targeting using epifluorescent imaging. The most common variants located to secreting endosomes and membrane in HC11 cells. Incorrect targeting of SLC30A2 causes mislocalization. It may be possible to identify mothers carrying risk genotypes for infant zinc deficiency.

The proportionality constant in Darcy's Law is called hydraulic conductivity (K), and it is the most fundamental parameter to groundwater studies. There are a number of in situ and laboratory techniques employed to determine K, one of which is falling head permeameter (FHP). In FHP, determining K involves two steps: measuring hydraulic head change over time and calculating the K value. In the past, calculating K was done using Darcy's Law, which states linear correlation between the flux and the hydraulic gradient, but this is only true when the inertial forces are negligible at small velocities. At higher velocities, flow becomes unsteady because of the change over time in flow magnitude and hydraulic gradient, which requires mass conservation law to be combined with Darcy’s Law and eventually leads to Laplace’s equation for an incompressible matrix. If the media is compressible, specific storativity should be taken into account, as well. In this study, we investigated the transiency of flow in FHP tests by analyzing the effect of specific storativity on K calculations. We have developed a new semi- analytical solution for transient flow in FHP in Laplace domain and used the de Hoog algorithm to attain the inverse Laplace transform of this solution to yield solutions in time domain. We have also provided some analysis and a comparison of steady-state solution along with using experimental data and the data from the literature to analyze the solution. Upon these, we concluded that the transient flow in falling-head tests has minimal effect in general, although using the transient solution provided may improve the accuracy without a major effect.

Experiments in the study of transient aerodynamics typically require pressure measurements with a high spatial and temporal resolution. Existing commercial pressure transducers are expensive and they provide a spatial resolution only on the order of millimetres. The full bandwidth of commercial devices (which extends to around 200 kHz) can only be utilised by exposing the transducer to the flow environment with very little thermal or mechanical protection. If insufficient protection is provided, the expensive commercial devices are likely to be damaged. Inexpensive pressure sensors based on extrinsic Fabry-Perot fibre optic interferometry are capable of measurement with a high spatial and temporal resolution. Thermal protection or isolation for these sensors is still required, but they can be exposed directly to the flow if the sensors are disposable (low cost). Excessive thermal or mechanical protection is not required for these sensors because the damaging heat transfer and particle impacts that may occur in transient aerodynamic facilities generally occur after the useful test flow. In this dissertation, a variety of construction techniques for diaphragm-based Fabry-Perot fibre optic pressure sensors were investigated and the advantages and disadvantages of all techniques are compared. The results indicate that using a zirconia ferrule as the substrate, a liquid adhesive as the bonding layer, and a polished copper foil as the diaphragm provide the best results. It is demonstrated that a spatial resolution on the order of 0.1 mm and a bandwidth to more than 100 kHz can be achieved with such constructions. A variety of problems such as hysteresis, response irregularity, low visibility and sensor non-repeatability were observed. By using a thinner bonding layer, a larger bonding area, longer cavity length, increased calibration period, and applying load cycling to the diaphragm, the hysteresis was minimized. Sensor response irregularity was also minimized using a polished diaphragm. Visibility increased to about 90% using active control of the cavity length during the construction process. Non-repeatability was found to be a consequence of adhesive viscoelasticity and this effect was minimized using a thin layer of adhesive to bond the diaphragm to the substrate. Due to the effects of adhesive viscoelasticity, the pressure sensors indicate an error of up to 10% of mean value for the reflected shock pressure. This error could not be further reduced in the current sensors configuration. Some new configurations are proposed to decrease the effect of sensor non-repeatability. The effect of pretensioning the diaphragm was investigated analytically but the results do not indicate any considerable advantage for the levels of pretension likely to be achieved in practice. However, the results do indicate that pretension effects caused by an environmental temperature change can damage the sensor during storage. The effect of the initial diaphragm deflection on the sensor performance and temperature sensitivity was modelled and the results show that an initial diaphragm deflection can improve the sensor performance. The effect of the thermal isolation layer on the sensor performance was also investigated and the results show that for a shock tube diaphragm bursting pressure ratio up to 5.7, heat transfer does not contribute to sensor errors for the first millisecond after shock reflection. However, it was found that the use of a thin layer of low viscosity grease can protect the sensor for about 20 ms while only decreasing its natural frequency by typically 17%. The grease layer was also found to decrease the settling time of a low damping ratio sensor by 40%. The sensor was successfully employed to identify an acoustic disturbance in a shock tube.