Transportation has become an essential part of people's lives to move from one place to another, increasing the transport mobility for passengers. Therefore, road transport accounts for the second largest contributor to greenhouse gas emissions in Europe, and to increased congestion. Hence, reducing the share of car use can lead to reduced greenhouse gas emissions, congestion, etc. However, not all types of car travel could be reduced, therefore there is a need to maintain certain transport demand. Instead, passenger transport could be made more efficient, such as utilizing time, space, vehicles, and fuel in the movement without any waste. Efficiency in passenger transport could be achieved through a variety of interventions that aim to decrease the share of car use. The aim of this master thesis is to state interventions that reduce the share of car use and achieve efficient passenger transport. / <p>Examensarbetet är utfört vid Institutionen för teknik och naturvetenskap (ITN) vid Tekniska fakulteten, Linköpings universitet</p>

Acute colitis was induced by exposing male SD rats to 4% DSS in drinking water for 8 days. Rats were divided into four groups as follows: DSS group---DSS-induced colitis; DSS + SRE group---SRE, 100 mg/kg/day in addition to DSS; Ctr + SRE group---SRE alone; and Ctr group---sham control group. The colon damage was elucidated by macroscopic, histological, electrophysiological and biochemical assessment. Orally administered SRE significantly reduced the colonic damage in all four aspects. However, baicalein did not show similar effect in the same experiment. / In summary, our finding indicated that both SRE and its major flavonoid, baicalein, could stimulate chloride secretion in human colonic T84 cells and mucosa freshly isolated from human colon. Although SRE was effective in treating acute DSS-induced ulcerative colitis, baicalein is unlikely the active anti-inflammatory component of SRE. Nevertheless, the results demonstrated that this TCM has a scientific basis for its effectiveness. Our data support further evaluation of the therapeutic potential of SRE for the treatment of IBD. / In TCM, Scutellariae radix and Coptidis Rhizoma (CR) derived compounds have been frequently used for gastroenteritis and secretory diarrhea. Our laboratory findings suggested that the major flavonoid component of SR, baicalein, stimulates chloride secretion in rat distal colon, probably via CFTR activation (Ko et al., 2002). In contrast, limited information about the cellular mechanism of chloride secretion induced by SR in human colonic epithelia is available. Therefore, the effect of Scutellariae radix extract (SRE) on electrolyte transport in a human colonic epithelial cell line, T84, was examined using the short-circuit current (ISC) technique. Results demonstrated that SRE stimulated a Cl--dependent secretion across T84 cells, probably via both Ca2+- and cAMP-mediated pathway. / On the other hand, the cellular mechanism of baicalein-induced Cl - secretion in T84 cells was further investigated. It was found that the secretory mechanisms involve protein kinase A (PKA)-, adenylate cyclase (AC)- and luminal cAMP-dependent Cl- channels, most likely cystic fibrosis transmembrane conductance regulator (CFTR) and serosal 293B-sensitive K + channels. However, the action of baicalein cannot be solely explained by its cAMP-elevating effect. In addition, the effect of baicalein could be potentiated by the inhibition of mitogen-activated protein kinase (MAPK) and phosphoinositide-3 kinase (PI3K). Furthermore, it was found that inhibition of protein kinase C (PKC) delta limited the baicalein-induced chloride secretion. / Our laboratory has found that baicalein (Ko et al., 2002 and Yue et al., 2003) stimulates chloride secretion in rat distal colon and human colonic T84 cells. As it is known that responses in the animal model or the cell line may not completely reflect the in vivo physiology, it is important to study the above responses in human colon. With scarce supply of freshly isolated human colonic mucosa, the results showed that the effect of SRE and baicalein on ion transport in human samples is similar to that obtained in T84 cell line and rat model. / Scutellariae radix (SR) is the dry root of Scutellariae baicalensis Georgi (Huangqin). SR has been employed for centuries as a traditional Chinese medicine (TCM) for various purposes. It contains a large amount of flavonoids such as baicalein, baicalin, and wogonin, which possess a number of beneficial bioactivities including anti-oxidant, anti-bacterial and anti-inflammatory, etc. / Ulcerative colitis (UC), an inflammatory bowel disease (IBD), has been known for more than half a century. Recent studies have shown that two flavonoids derived from SR, baicalein and wogonin, might alleviate the symptoms of IBD. Moreover, SR is the major component of Hange-shasshin-to (HST), one of the Chinese herbal formulas, which has been reported to suppress the pathogenesis of IBD. The above scientific background led us to examine the effect of SRE administration on DSS-induced colitis in rats in a way to evaluate new treatments potentially applicable to UC in humans. / Chung, Ho Lam. / "August 2007." / Adviser: W. H. Ko. / Source: Dissertation Abstracts International, Volume: 69-02, Section: B, page: 0925. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.

A seasonal and long-term analysis of the vertical structure of currents in the nearshore is conducted to determine the role of the wind in driving currents and consequently affecting littoral transport processes. Approximately ten years (January, 2002 – October, 2011) of nearshore current profiles are examined using the data collected with an Acoustic Doppler Current Profiler (ADCP) installed off of Spessard Holland North Beach Park located in Melbourne Beach, Florida. Additionally, wind data collected with a directional anemometer from September, 2002, until October, 2008, are used to further characterize the long-term hydrodynamic forcing. With the shoreline oriented nominally 17o west of magnetic north, both the current profiles and the wind vectors have been rendered into longshore and cross-shore components. The water level record from a NOAA tide station located at the Trident Pier at nearby Port Canaveral is utilized in establishing the water depth and conditioning the data for statistical analysis. Monthly mean vertical profiles reveal that during the winter months the surface currents are usually toward the south, and toward the north in the summer. In spring and fall, they are mixed, demonstrating a clear seasonality in both direction and intensity of the longshore current. Subjecting the longshore and cross-shore current data to Empirical Orthogonal Function Analysis reveals that the first spatial Eigenfunction accounts for more than 98% of the variability in the vertical profile of the longshore current, and more than 86% of the variability in the profile of the cross-shore current. However, there is a rotation of the current to the right (clockwise) with the rotation angle increasing and the variance decreasing with depth below the surface. The spiral structure of the water column follows a surface Ekman veering, but for very shallow water. The upper layer of the current is almost aligned with the direction of the wind. Monthly correlations between 2-hour average time series of longshore current and 2-hour average time series of wind speed reveal the seasonal patterns of the wind and longshore current in which the upper layer of the water column is highly correlated with the longshore component of the wind speed for most of the year and slightly less correlated for the lower layer of the water column. Most importantly, on average, wave height (Hmo) is larger when the longshore current is heading to the south (Hmo=0.95 m) than when the current is going to the north (Hmo=0.73 m). Additionally, there is a stronger correlation between southerly directed currents and incident wave energy flux than northerly directed currents and wave energy flux. These results indicate that the net long-term north-to-south sediment transport known to characterize the region is heavily influenced by wind-driven currents.