Thesis (MScFor)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: In South Africa fertilizer applications at establishment is a common practice in the forestry industry. Recommendations are based on past research with conventional sources (CV) and as a result there have only been slight improvements in additional plantation production over recent years. The objective of this study was to investigate initial stand responses in terms of leaf are index (LAI), foliar nutrient content, biomass index (BI) and volume growth to the application of controlled release fertilizers (CRF) at re-establishment. Nitrogen (N), phosphorus (P) and potassium (K) were applied in a three way factorial combination to Pinus radiata at planting on a site in the Western Cape. The design was replicated five times across the study area. N (CRF) and P (CV) were applied in a two way factorial combination at planting to two Eucalyptus grandis x urophylla hybrids and Eucalyptus dunnii across three sites, one ex-agricultural and two re-establishment sites, on the Zululand coastal plain and the Midlands region. The design was replicated nine times on each study site. Harvesting residues were burnt on the eucalypt sites prior to establishment and may have had an impact on the responses found. An outbreak of Fusarium circinatum on the P. radiata site resulted in unexpected and extremely variable responses. An attempt to quantify the effect of the disease had limited success. The mean BI of the control treatment reached 25. The best CV and CRF treatments yielded improvements of 42 % and 83 % in BI over the control respectively, with only the CRF treatment difference being significant. Foliar analysis results revealed marginal to deficient concentrations of P and Mg being alleviated by the treatments in question. On the KZN Zululand sites there was a marked response of the hybrids to N with P having an additive effect on volume growth, LAI and foliar N content. Application of 120 g N and 20 g P per tree on the ex-agriculture site produced a volume of 8 m3 ha-1 at one year of age, a significant increase of 118 % and 80 % over the control and best CV treatment respectively. Application of 80 g N and 20 g P per tree, on the re-establishment site, yielded a volume of 24.6 m3 ha-1 at one year which equates to a significant 39 % and insignificant 7 % additional volume at one year over the control and best CV treatment respectively. A non-significant suppressive effect was found with 20 g CV P application only. At the KZN Midlands site, the major early response in height were to P application. Applications of 20 g CV P per tree, resulted in a mean height of 162.6 cm at seven months of age, a significant 28 % higher than the control. There was no significant effect of 80 g CRF N and 20 g CV P per tree respectively. CRF N applications up to 120 g per tree provide additional growth over recommended CV applications on coastal Zululand sites with low organic carbon (OC) content. On the KZN Midlands site with higher OC and clay content, early responses were limited to P fertilization only regardless of the fertilizer source. / AFRIKAANSE OPSOMMING: Dit is algemene praktyk in die Suid Afrikaanse bosbou industrie om kunsmis tydens aanplanting toe te dien. Hierdie aanbeveling is gebasseer op navorsing resultate met konvensionele bronne (CV), gevolglik was slegs ‘n klein toename in bykomende plantasie produksie gemeet die afgelope paar jaar. Die doelwit tydens hierdie studie is om die aanvanklike reaksie van die bome in terme van blaar oppervlak indeks (LAI), blaar-voedingstof inhoud, biomassa indeks (BI) en volume op die toediening van beheerde vrylating kunsmis (CRF) tydens aanplanting vas te stel. Stikstof (N), fosfor (P) en kalium (K) is in drie-ledige kombinasie aan Pinus radiata op ’n plantasie in die Weskaap toegedien. Die ontwerp is vyf keer in die studiegebied herhaal. N en P is in twee-ledige kombinasie aan twee Eucalyptus grandis x urophylla hibriede en Eucalyptus dunnii op drie groeiplekke, een eks-landbou en twee eks-bosbou, op die Zoeloeland kusvlakte en in die Natalse Middellande toegedien. Die ontwerp is nege keer in elke studiegebied herhaal. Oesreste is voor aanplanting op die Eucalyptus groeiplekke verbrand. Die voorkoms van Fusarium circinatum op die P. radiata groeiplek het onverwagte en hoogs uiteenlopende reaksies tot gevolg gehad. ’n Poging om die effek van die siekte te kwantifiseer, was slegs gedeeltelik suksesvol. Die gemiddelde BI van die kontrole behandeling, was 25. Die beste CV en CRF behandeling het onderskeidelik 42 % en 83 % hoër BI as die kontrole groep gehad, waarvan slegs die CRF behandeling beduidend was. Blaarontleding het gewys dat daar marginale of ontoereikende konsentrasies van P en Mg was. In Zoeloeland het die Eucalyptus hibriede ’n beduidende reaksie op N en P getoon met meer volume groei, LAI en N inhoud. Die toediening van 120 g N en 20 g P per boom op die eks-landbou groeiplek het ’n volume van 8 m3 ha-1 op eenjarige ouderdom tot gevolg gehad. Dis beduidend beter met 118 % en 80 % onderskeidelik vir die kontrole en beste CV behandeling. Die toediening van 80 g N en 20 g P per boom op die hervestigde eks-bosbou groeiplek, het ’n volume van 24.6 m3 ha-1 op eenjarige ouderdom tot gevolg gehad. Dit is 39 % beduidend en 7 % onbeduidend addisionele volume op eenjarige ouderdom vir onderskeidelik die kontrole groep en beste CV behandeling. ’n Onbeduidende depressie effek is met die alleen toediening van 20 g CV P gevind. In die Natalse Middellande groeiplek het die toediening van P ‘n vroeë reaksie in hoogte groei veroorsaak. Die toediening van 20 g CV P per boom, het ’n gemiddelde hoogte van 162.6 cm op die ouderdom van sewe maande tot gevolg gehad. Dit is 28 % beduidend hoër as die kontrole. Die toediening van 80 g CRF N en 20 g CV P per boom was onbeduidend. Toedienings van CRF N tot en met 120 g per boom het in die kusgebiede van Zoeloeland met ‘n lae organiese koolstof (OC) inhoud, groter groei as die aanbevole CV toedienings gehad. Die Middellande groeiplek met ’n hoër OC en klei inhoud, was die vroeë reaksie alleenlik beperk tot P bemesting.

Department of Geography and Geo-Information Sciences / MENVSC

The pomegranate fruit is one of the high valued crops, but there is insufficient information regarding the fruit properties in South Africa. The aim of the study was to evaluate the physico-chemical properties as well as total phenols, anthocyanin, antioxidant, organic sugars and acids of cultivar Wonderful on three locations of the Western Cape. This study was conducted on mature pomegranate fruits harvested in the 2012 and 2013 seasons. Fruit weight (g), length (mm), and width (mm), peel/aril colour and total arils weights (g) were measured. Fruits were also analysed for total soluble solids (TSS) or °Brix), titratable acidity (TA) and juice pH. Results of the study showed that there were significant differences in all measured factors with the exception of % aril yield between the three locations. Though varied per season, fruits produced at Bonnievalle had better physical and chemical properties than at the other localities. With the exception of Aril hue angle, all measured parameters had significant interaction effect regardless of locality (P<0.05). Total soluble solids content varied from 16.0–17.3 (°Brix), pH values from 2.7–3.0, titratable acid content varied from 1.3–1.7 and maturity index from 9.7–13.4. The anthocyanin, total phenols and antioxidant were in order of 772–1134; 1611–1834 and 12.57–14.84. Organic acids (Citric and Malic) showed differences while Acetic acid was not significant in all areas and organic sugar (fructose, Glucose and Sucrose) all had significant differences. It can also be concluded that changes in colour of peel and arils of pomegranate (cv. Wonderful) was mostly as a result of seasonal variation as well as growing area as evident by the interaction between both main factors. / Agriculture / M.Sc (Agriculture)

The pomegranate fruit is one of the high valued crops, but there is insufficient information regarding the fruit properties in South Africa. The aim of the study was to evaluate the physico-chemical properties as well as total phenols, anthocyanin, antioxidant, organic sugars and acids of cultivar Wonderful on three locations of the Western Cape. This study was conducted on mature pomegranate fruits harvested in the 2012 and 2013 seasons. Fruit weight (g), length (mm), and width (mm), peel/aril colour and total arils weights (g) were measured. Fruits were also analysed for total soluble solids (TSS) or °Brix), titratable acidity (TA) and juice pH. Results of the study showed that there were significant differences in all measured factors with the exception of % aril yield between the three locations. Though varied per season, fruits produced at Bonnievalle had better physical and chemical properties than at the other localities. With the exception of Aril hue angle, all measured parameters had significant interaction effect regardless of locality (P<0.05). Total soluble solids content varied from 16.0–17.3 (°Brix), pH values from 2.7–3.0, titratable acid content varied from 1.3–1.7 and maturity index from 9.7–13.4. The anthocyanin, total phenols and antioxidant were in order of 772–1134; 1611–1834 and 12.57–14.84. Organic acids (Citric and Malic) showed differences while Acetic acid was not significant in all areas and organic sugar (fructose, Glucose and Sucrose) all had significant differences. It can also be concluded that changes in colour of peel and arils of pomegranate (cv. Wonderful) was mostly as a result of seasonal variation as well as growing area as evident by the interaction between both main factors. / Agriculture / M. Sc (Agriculture)

MSc (Botany) / Department of Botany / Since time immemorial plants have traditionally been used for various purposes in many parts of the world including Vhembe district, Limpopo province of South Africa. The use of C. abbreviata plants at Matsa village of Vhembe district also contributes significantly to the livelihood of the community. However, little work has been made in the past to properly document and promote the knowledge. The purpose of the present study was to record and analyse the ethnobotanical knowledge of C. abbreviata within the people of Matsa village around Nzhelele in Vhembe district. Semi-structured questionnaires were administered on 50 informants from different households selected randomly to gather data regarding the local name of C. abbreviata, the uses and its availability. Significantly higher number of medicinal use was reported by elderly people including both men and women as compared to middle-aged, younger people. Other use categories included firewood, furniture and joinery production and shade for people in the area. Awareness on the importance of the species should therefore be created amongst the people, especially the young ones. Understanding the ethnobotanical knowledge of indigenous species within an area is crucial towards development of its management plan. / NRF

MSCAGR (Animal Science) / Department of Animal Science / Forage and browse legumes play an important role in sustaining livestock in small holder farming systems in the tropics, mainly as a result of their contribution to economic and environmental sustainability of livestock production. The study was conducted to determine the chemical composition, ruminal degradability and in vitro digestibility of dry matter (DM) and crude protein (CP) of Dichrostachys cinerea and Bauhinia thonningii leaves. The browse tree leaves were harvested in the wild in Shayandima, Limpopo province. The leaves were collected, oven-dried, milled to pass through a 1.0 mm sieve and analysed for chemical composition in the Animal Science Nutrition Laboratory, at the University of Venda. The browse tree leaves were analysed for DM nitrogen, neutral detergent fibre (NDF) and acid detergent fibre (ADF). Approximately 5 g of leaf sample milled to pass through through a 1 mm sieve were placed in nylon bags (external dimension: 6 × 12 cm, pore size of 41 μm) and incubated in duplicates for 0, 4, 8, 16, 24, 48, 72, 96 and 120 hours periods in the rumen of three cannulated Bonsmara steers. The residues were then analysed for DM and nitrogen. Parameters to describe the dynamics of ruminal degradability of DM and CP were obtained by fitting the data on the exponential equation P = a + b (1 - e-ct) using NEWAY computer program, where “a” is the rapid degradable fraction, “b” is the slow degradable fraction and “c” is the outflow rate. The in vitro DM and CP degradability of rumen undegradable residue collected after 24 and 48 hour incubation was determined by sequential in vitro digestion in pepsin (abomasal) and pancreatin (small intestine) solutions. DM and CP content differed significantly (P ˂ 0.05). D. cinerea leaves had higher levels of DM and CP content than B. thonningii leaves. DM and CP disappearance increased (P < 0.01) as the incubation period increased. There was no difference (P > 0.05) in soluble fraction ‘a’ and ‘b’ of DM of the two species. The CP components for both fraction ‘a’ and ‘b’ differed significantly (P < 0.01) for CP among the two species. There was significant difference (P < 0.01) in post-ruminal digestibility among the two species. CP digestibility of B. thonningii and D. cinerea leaves was reduced (P < 0.01). In conclusion, B. thonningii and D. cinerea leaves showed significant difference based on their fermentation kinetics and in vitro digestibility, suggesting a good nutritional quality which can be used as protein source for ruminants in dry season and supplement to low-quality diets. / NRF