Four studies were conducted to determine the effect of synchronization of protein and starch degradation on nutrient utilization, microbial protein synthesis and milk production in dairy cows. In Experiment 1, five cereal grains and five protein supplements were compared for extent of solubility and degradability of their starch and nitrogen fractions. Results indicated large differences which permitted their ranking from high to low degradability as follows: grains, oats > wheat > barley > corn > milo protein supplements, soybean meal > cottonseed meal, (CSM) > corn gluten meal > brewers dried grains, (BDG) > blood meal. In Experiment 2, the five grains were incubated for varying times in vitro (with added amylase) or in situ to determine rate and extent of degradation of dry matter, crude protein and starch. Results showed that rate of starch degradation followed a similar, but slightly different trend than in trial 1 (wheat > barley > oats > corn > milo). Rates for DM and CP degradation were similar than those for starch. In Experiment 3, high (barley, HS) and low (milo, LS) degradable starch sources were combined with a high (CSM, HP) and a low (BDG, LP) degradable protein sources to formulate four diets; HSHP, HSLP, LSHP and LSLP. Diets were fed to 32 cows, starting two to four weeks postpartum, for a 60-d milk production and digestibility study. Apparent digestibility was calculated using chromium oxide. Organic matter digestibility was higher (P < .05) was found in nutrient output to the small intestine among diets and microbial CP synthesis was higher (P > .05) for barley diets.

Four experiments were carried out with laying hens to evaluate the effects of body weight, age, dietary protein, and tallow levels on performance, nutrient intake and energy utilization. In the first experiment, Single Comb White Leghorn birds were divided into four body weight groups at the onset of egg production. Voluntary feed intake was 18% less for the lightest (1.39 kg) in comparison with the heaviest group (1.83 kg). Egg weight was directly related to body weight with the heavy birds producing an average egg weighing 65.3 g and the lightest birds having an average egg weight of 58.9 g. Feed conversion was also significantly better for the lighter birds. Maintenance requirements for the heaviest and lightest birds were 60.5 and 57.9% of metabolizable energy consumed, respectively. Metabolizable energy intakes above maintenance were 131 kcal/d for the heavy birds and only 119 kcal for the light group. A composite regression analysis indicated a maintenance requirement of 127.7 kcal/d/kg('0(.)75) and an energetic efficiency of 75.2% for the conversion of metabolizable energy to net energy. In order to evaluate the effects of age and body weight on laying hen performance, the last three experiments were designed using old, molted, and young hens which were divided into the heaviest and lightest body weight groups. The old birds were 72 weeks old, the molted birds were 106 weeks old, and the young birds were 27 weeks old at the start of the studies. In each experiment the birds were fed ten experimental diets with 12, 14, 16, 18, and 20% dietary protein in combination of 1 and 4% supplemental fat. Egg output was increased with the supplementation of tallow in only the young birds; while energy retentions were improved in the old and molted birds with fat feeding. Average energy retentions per kg physiological body weight were 58.8, 41.7, and 38.6 kcal for the young, molted, and old hens, respectively. The light-bodied birds showed 9% better gross energetic efficiencies than the heavy-bodied birds. Estimated daily protein intake requirements were 16.8, 13.3, and 12.8 g/d to support production levels of 84, 64, and 66% for the young, old, and molted birds, respectively.

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Call number: LD2668 .T4 1986 G66 / Master of Science / Animal Science and Industry

Estudos referentes à eficiência de absorção e utilização de potássio (K), bem como a definição de valores críticos de K no solo e na planta para cultivares de soja com tipo de crescimento determinado (TCD) e indeterminado (TCI) são fundamentais para a seleção de genótipos quanto às exigências nutricionais, possibilitando o posicionamento desses materiais para o cultivo em distintos ambientes edáficos. Este trabalho teve como objetivo avaliar a eficiência de absorção de K (rubídio - Rb) por meio dos parâmetros cinéticos de absorção (Vmáx, Km e Cmín) e da morfologia radicular; a eficiência de utilização de K por meio do transporte e redistribuição de K (e de Rb) nas partes das plantas; e definir valores críticos de K no solo e nas folhas utilizadas para diagnose em soja com TCI. Os dois primeiros experimentos (cinética de absorção de Rb e morfologia radicular, e transporte e redistribuição de K/Rb) foram desenvolvidos em condições de casa de vegetação no CENA/USP, no período de outubro de 2012 a janeiro de 2013, e o terceiro experimento, foi desenvolvido em condições de campo na Embrapa Soja, no período de setembro de 2010 a março de 2013. Para os dois primeiros experimentos, foi utilizado um cultivar de soja com TCD (TMG 1066 RR) e outros três com TCI (VMAX RR, NA 5909 RG e BRS 360 RR) e para o último experimento, utilizou-se os cultivares de soja com TCI VMAX RR e BRS 360 RR. Houve variação genotípica quanto aos parâmetros morfológicos de raízes, exceto para o diâmetro radicular. Os parâmetros cinéticos de absorção Vmáx, Km e Cmín variaram em função dos cultivares utilizados em cada concentração de Rb. Contudo, não foram verificadas diferenças quanto a eficiência de absorção de K entre cultivares do grupo TCD e TCI. Houve interação significativa entre genótipos e tratamentos de Rb para as variáveis produção de grãos, putrescina, acúmulo de K e Rb, redistribuição de Rb, transporte a longa distância e eficiência de utilização de K. Os cultivares TMG 1066 RR e VMAX RR possuem maior eficiência em produção e utilização de K. Não foram verificadas diferenças quanto ao acúmulo e redistribuição de K para cultivares TCD e TCI. No último experimento, foram verificados sintomas de deficiência de K nos dois cultivares de soja a partir do início da formação de vagens e enchimento de grãos. O NC externo de K para o cultivar VMAX RR e BRS 360 RR foi de, respectivamente, 0,38 e 0,29 cmolc dm-3, enquanto que o NC interno de K para os mesmos cultivares foi de 25,4 e 21,3 g kg-1, respectivamente. Os valores de NC externo e interno obtidos neste estudo são superiores aos verificados na literatura para genótipos de soja com tipo de crescimento determinado (TCD) / Studies on the potassium use efficiency (KUE), potassium uptake efficiency (KUpE) as well as the definition of critical values of K in soil and plant for soybean cultivars with determinate growth type (DGT) and indeterminate (IGT) are fundamental to the selection of genotypes to nutritional requirements and allow placement of these materials for cultivation in different edaphic environments. This study aimed to evaluate the K (and Rubidium - Rb) uptake efficiency by uptake kinetics (Vmax, Km and Cmín) assessment and root morphology, the K use efficiency (KUE) through the transport and redistribution of K (and Rb) in plant parts, and define critical values of K in the soil and leaves used for diagnosis for soybeans with IGT. The first two experiments (uptake kinetics of Rb and root morphology, transport and redistribution of K/Rb) were carried out in greenhouse conditions at CENA/USP, from October 2012 to January 2013, and the third experiment was developed under field conditions at Embrapa Soybean, from September 2010 to March 2013. For the first two experiments, one cultivar with DGT (TMG 1066 RR) and three with IGT (VMAX RR, NA RG 5909 and BRS 360 RR) was used, and for the last experiment was used the soybean cultivars with IGT VMAX RR and BRS 360 RR. There was genotypic variation in morphologic parameters of the roots, except for the root diameter. The uptake kinetics parameters Vmax, Km and Cmín ranged among the cultivars on each concentration of Rb. However, no difference was observed on the K uptake efficiency (KUpE) among the cultivars from DGT or IGT group. There was a significant interaction between genotypes and treatments of Rb for the variables grain mass yield, putrescine, K and Rb accumulation, Rb redistribution, long distance transport of K and K use efficiency (KUE). The TMG 1066 RR and VMAX RR cultivars have a greater efficiency in production and use of K. No differences were found concerning the accumulation and redistribution of K for DGT and IGT cultivars. Symptoms of K deficiency were found in two soybean cultivars from the beginning of pod formation and grain filling. The soil critical levels of K to VMAX RR and BRS 360 RR genotypes were 0.38 and 0.29 cmolc dm-3, respectively, while the plant-tissue critical levels of K for the same cultivars were 25.4 and 21.3 g kg-1, respectively. The soil and plant-tissue critical level values obtained in this study are higher than those in the literature for genotypes with determinate growth type (DGT)

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