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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Ocorr?ncia da infec??o por Toxoplasma gondii e avalia??o da imuniza??o em caprinos do sert?o do cabugi, Rio Grande do Norte

Medeiros, Andr?a Dantas de 31 March 2010 (has links)
Made available in DSpace on 2014-12-17T14:10:22Z (GMT). No. of bitstreams: 1 AndreaDM_DISSERT.pdf: 1136289 bytes, checksum: 4971fd9b2aab3f08935061c736892689 (MD5) Previous issue date: 2010-03-31 / Toxoplasmosis is one zoonosis caused by Toxoplasma gondii protozoan. Goats, amongst the production animals, are one of the species most susceptible to this parasite, being one them main involved agents in ovine and goat abortions, determining great economic losses and implications for public health, since the presence it parasite in the products of goat origin, consist in one of the main sources of infection for the man. In this study 244 blood samples in 8 farms situated in 4 cities from the Sert?o do Cabugi region, Rio Grande do Norte State, northeast of Brazil and, tested by ELISA assay. The results had shown a prevalence of 47.13% for anti- T. gondii antibodies and a significant association between positivity and variable evaluated as age, locality and property. The IgG avidity assay evaluated in 115 positive samples was carried to discriminate acute and chronic infection. Twelve samples (10.4%) had presented antibodies of low avidity while 103 (89.6%) presented high avidity antibodies; indicating that most of the animals was precocious exposure to the parasite. Significant difference was verified only for the variable sex. We also evaluate the capacity of recombinant adenoviruses codifying SAG1, SAG2, SAG3 and CMV in inducing activation of specific immune response in goat. These 109 animals received 109 pfu of the AdSAG1, AdSAG2, AdSAG3, AdCMV or PBS in vaccine protocol with 3 immunizations. Serum samples of the each animal, before and after mmunization, had been submitted to the ELISA. The results demonstrate that the immunizations had induced the production of IgG antibodies specific against T. gondii proteins / A toxoplasmose ? uma zoonose causada pelo protozo?rio Toxoplasma gondii. Os caprinos, dentre os animais de produ??o, s?o uma das esp?cies mais suscet?veis a este parasito, sendo um dos principais agentes envolvidos no abortamento de ovinos e caprinos, determinando grandes perdas econ?micas e tendo implica??es para sa?de p?blica, j? que a presen?a do parasita nos produtos de origem caprina, constituem-se em uma das principais fontes de infec??o para o homem. Neste estudo foram coletadas 244 amostras de sangue em 8 fazendas situadas em 4 munic?pios da regi?o do Sert?o do Cabugi, Estado do Rio Grande do Norte, regi?o Nordeste do Brasil e testadas atrav?s do Ensaio Imunoenzim?tico (ELISA). Os resultados obtidos mostraram uma preval?ncia de 47,13% para anticorpos anti-T. gondii e uma associa??o significativa entre positividade e as vari?veis avaliadas tais como idade, localidade e propriedade. Foi realizado o ensaio IgG de avidez em 115 amostras positivas com o intuito de discriminar infec??o aguda e cr?nica. Doze amostras (10,4%) apresentaram anticorpos de baixa avidez enquanto 103 (89,6%) anticorpos de alta avidez; indicando que a maior parte dos animais foi exposto ao parasita precocemente. Foi verificada diferen?a estat?stica significativa apenas para a vari?vel sexo. Avaliamos tamb?m a capacidade de adenov?rus recombinantes codificando SAG1, SAG2, SAG3 e CMV em induzir ativa??o de resposta imune em caprinos. Estes animais receberam 109 pfu de AdSAG1, AdSAG2, AdSAG3, AdCMV ou PBS em protocolo vacinal com 3 imuniza??es. Amostras de soro obtidas dos animais, antes e ap?s cada imuniza??o, foram submetidas ao ELISA. Os resultados demonstram que as imuniza??es induziram a produ??o anticorpos IgG espec?ficos contra as prote?nas de T. gondii

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