El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado. / This work describes the design of an external cleaning system for fire hoses with an external polyester or rubber jacket. This device is intended as a tool for simple and effective external cleaning. Within the capabilities of the equipment is the washing and winding of a hose. This is made up of a washing chamber, which contains a group of sprinklers inside with a brushing system and a winding mechanism with a platform designed to carry out the type of winding snail or single donut. Cleaning can be applied to both types of coatings with the action of a selector switch, the variables involved are adjusted accordingly. The system aims to perform its functions without damaging the composition of the hose lining material, in addition to reducing the space required during cleaning.

Column flotation cells have become increasingly popular in the coal industry due to their ability to improve flotation selectivity. The improvement can be largely attributed to the use of froth washing, which minimizes the nonselective entrainment of ultrafine minerals matter into the froth product. Unfortunately, the practice of adding wash water in conventional flotation machines has been largely unsuccessful in industrial trials. In order to better understand the causes of these failures, a detailed in-plant test program was undertaken to evaluate the use of froth washing at an operating coal preparation plant. The tests included detailed circuit audits (solid and liquid mass balances), salt tracer studies, and release analyses. The data collected from these tests have been used to develop criteria that describe when and how froth washing may be successfully applied in industrial flotation circuits. A second series of tests was developed to look at other alternatives to froth washing and their effectiveness. This involved two-staged flotation circuitry. A two-staged approach was developed because the existing flotation cells did not have enough residence time to support froth washing. The process owner wanted to evaluate possible alternatives to column cell flotation. The testing included release analysis testing as well as a detailed series of tests with percent solids control to the secondary flotation unit. / Master of Science

The relationships between water, sanitation, and hygiene (WASH) interventions and malnutrition markers such as stunting, wasting, and underweight have been poorly characterized until recently. There is a need to identify interventions that may play a role in these relationships to improve nutritional status of children under 5 years old. In 2013, Zambia was not on track to meet MDG 7c, to halve the proportion of the population without sustainable access to safe drinking water and basic sanitation. To address this goal, the Zambian government implemented the Zambia Sanitation and Hygiene Program (ZSHP). The goal of the program was to reduce WASH-related diseases in rural areas by promoting community wide sanitation using community-led total sanitation (CLTS), legal enforcement, and hygiene promotion. An important component of the ZSHP is CLTS, which has been implemented in a majority of districts. The overall study aims to explore the effectiveness of a CLTS intervention on coverage and quality of household sanitation facilities, defecation behavior, and child health in a rural setting in Zambia. A secondary outcome that was measured in the analysis of the overall study was the effect of CLTS on stunting, wasting, and underweight in children under the age of 5. These three outcomes are the focus of this thesis. It was hypothesized that these three outcomes would decrease in prevalence with an increase in sanitation coverage resulting from the CLTS program. Cross-sectional anthropometric and household data for Zambian children under 5 and their households from baseline and end line surveys were analyzed to determine any association between multiple WASH indicators and nutritional status. The baseline survey was conducted in 2013 and the end line in 2016. Only households with at least one child under the age of 5 were surveyed at both baseline and end line. Primary caretakers were interviewed during home visits to assess sanitation resources, child-feeding practices, and the health status of their children under the age of 5. In addition, anthropometric data of children under the age of 5 was taken to assess their nutritional status of their children. The prevalence of underweight, stunting, and wasting at end line was 12.9%, 40.9%, and 7.4% respectively, all of which showed statistically significant decreases from baseline measurements (p=≤0.001, 0.03, and ≤0.001 respectively). Predictors of undernutrition were analyzed using logistic regression controlling for age and sex. Nutritional status of children under 5 years of age was found to be associated with several WASH indicators. Children who were taken to a clinic during an episode of diarrhea and children who lived in households with an improved water source had 46% and 26%, respectively, decreased odds of being stunted. Children who lived in households that were close (<4 min walk) to a water source had a 73% decrease in odds of being wasted. Finally, children who had diarrhea in the two weeks before the survey had 63% and 42% increased odds of being underweight or wasted respectively. Focus areas of the program have increased coverage of key indicators of sanitation and hygiene but a relatively high prevalence of sanitation- and hygiene-related diseases remain. These rural areas have high rates of reported diarrhea, acute respiratory illness, and stunting among young children even though most have had exposure to the ZSHP activities. With increased focus, not only on better human sanitation, but also on household environment sanitation, the prevalence of disease and malnutrition will start to decrease and we will begin to see healthier communities in Zambia.

The dynamics and function of the actin cytoskeleton depends on polymerization and branching of actin filaments, an event that is stimulated by Arp2/3. Arp2/3-dependent branching is closely linked to the pentameric WASH complex which consists of WASH, strumpellin, SWIP, CCDC53 and FAM21. WASH complex is associated mainly with endosomes. It was traditionally localized to retromer-coated domains of early endosomes which enable sorting and recycling of endocytosed material. However, latest scientific data extend the role of WASH complex to other endosomal or even non-endosomal sites. Of all the subunits of the WASH complex, FAM21 is the most prominent hub for protein-protein interactions, thanks to its long unstructured C-terminal domain. In my diploma thesis FAM21 was localized to early and late endosomes and lysosomes of U2OS human cell line. Dictyostelium discoideum was then used as a model organism to investigate FAM21 protein interactions as well as the proteins associated specifically with the C terminal domain of FAM21. Results of the study shed new light on the complex network of FAM21 interactions and question the long-standing theories on the function of WASH complex in cells. Powered by TCPDF (www.tcpdf.org)

Actin polymerization facilitated by the Arp2/3 complex plays a critical role in a wide range of cellular processes such as motility, endocytosis and cargo recycling. Activation and appropriate localization of the Arp2/3 complex is mediated by an interaction with the nucleation-promoting factor (NPF). WASH complex is the major endosomal NPF which plays a crucial role in the cargo recycling back to the trans-Golgi network (TGN) or plasma membrane. It is composed of five subunits: WASH1, SWIP, FAM21, CCDC53 and strumpellin. While WASH1 and FAM21 have been extensively studied, much less is known about strumpellin, a protein causally implicated in the onset of hereditary spastic paraplegia (HSP). This work focuses on strumpellin function in the cells, showing that only full-length protein incorporates into the WASH complex. In a strumpellin knock out cell line, we demonstrated that loss of strumpellin resulted in destabilization of the other WASH complex subunits. Still, an incomplete WASH complex without strumpellin was assembled. Cells also displayed enlarged endosomal subdomains and WASH complex nucleation activity on endosomes was largely diminished as assessed by loss of the actin patches. Finally, the absence of strumpellin was also accompanied by the accumulation of glucose transporter 1 (GLUT1)...

Tower Mountain Caldera is the main feature of an Oligocene volcanic field located in the Umatilla National Forest, eastern Oregon. It is perfectly suited to investigate models of rhyolite petrogenesis as all of the important rock components for evaluating generation models are present in a single location and thus are presumably related; basalts, intermediate igneous rocks (which consist of older plutons and younger volcanic rocks, which are ~coeval with rhyolites), metamorphic basement rocks of significant grade, and rhyolites of varying composition. The formation of the caldera produced the Dale Tuff, which comprises the intra-caldera and outflow facies. 40Ar/39Ar dating places the age of the tuff at 32.66 ± 0.36 Ma. Post-caldera rhyolites erupted along apparent ring fractures and elsewhere. Radiometric U-Pb dating of zircons from three of these rhyolites yielded ages of 32.167 ± 0.020 Ma (#CH07a), 31.798 ± 0.012 Ma (#TM5), and 31.426 ± 0.016 Ma (#CH08a). All rhyolites at Tower Mountain range from low to high silica varieties. Some of the post-caldera rhyolites are chemically similar to the Dale Tuff, such as sample CH07a, and have compositions typical of rhyolites of calc-alkaline volcanic centers (I-type rhyolites), while others are similar to A-type rhyolites (CH08a and TM5). The ages indicate that the calc-alkaline rhyolites were followed by the A-type rhyolites. The petrogenetic relationships between the various rocks types were evaluated. Partial melt modeling based on experimental melts produced from crustal material indicates that batch partial melting of metamorphosed high silica crustal material modified by the addition of more primitive mafic material by assimilation/contamination is the most likely source for the Tower Mountain rhyolites.

In order to avoid harmful substances ending up at the municipal sewage treatment plants, it is important to purify the wastewater at the source. One such source is the wheel washers available in many tire garages which are used to wash the wheels on our vehicles when we change between summer and winter tires. It is important to wash the wheels before storage, to extend the tires lifetime. The purpose of this study is to find a green innovative solution for reduction of emissions of heavy metals at the source, so that the sludge from the sewage treatment plants can be used as fertilizer. The target is to find a wheel wash method which leads to compliance with the guidelines for car washers, and that also provides a better washing result than with the existing method. Chemical analyzes of water samples have been performed and the cleanliness of the wheels has been checked visually. As there are no specific emission requirements for wheel washes, the results have mainly been compared with current guidelines for vehicle washes. A new washing and flocculation method have been developed during the study. The proposed new method is suitable as pretreatment of the water before discharging it, as it reduces metal emissions and provides better washing results compared to the old method. Significantly larger number of wheels may be washed in one session, which leads to a reduction of water- and energy consumption since the machine can be emptied and cleaned less often. For many years there has been an environmental adaption to eco-friendly car washers, so the opportunities should be good for wheel washers to win goodwill and market advantages by being profiled with an environmentally adapted washing method. / För att undvika att miljöskadliga ämnen hamnar i de kommunala reningsverken är det viktigt att rena avloppsvattnet vid källan. En sådan källa är de hjultvättmaskiner som finns på många verkstäder och som används för att tvätta hjulen på våra fordon i samband med skifte mellan sommar- och vinterdäck. Det är viktigt att tvätta hjulen före förvaring, för att förlänga däckens livslängd. Syftet med denna undersökning är att hitta en grön innovativ lösning för att främja uppströmsarbetet, dvs att rena utsläppen av tungmetaller vid källan, så att slammet från våra reningsverk kan användas som gödningsmedel. Målet är att finna en metod för hjultvätt som medför att riktlinjerna för fordonstvättar uppfylls, och som samtidigt ger ett bättre tvättresultat än befintlig metod. Kemiska analyser av vattenprover har utförts och renheten har kontrollerats visuellt. Eftersom det saknas specifika utsläppskrav för hjultvättar, har resultaten i första hand jämförts med gällande riktlinjer för fordonstvättar. En ny tvätt- och flockningsmetod har utvecklats under studiens gång. Den föreslagna nya metoden är lämplig som förbehandling av vattnet innan utsläpp, eftersom den sänker metallutsläppen samt ger ett bättre tvättresultat jämfört med den gamla metoden. Betydligt fler hjul kan tvättas per tvättomgång vilket innebär en minskning av vatten- och energiförbrukningen eftersom maskinen behöver tömmas och rengöras mer sällan. Under många år har det skett en miljöanpassning av fordonstvättar, så möjligheterna borde vara goda för att även hjultvättar ska kunna vinna goodwill och marknadsmässiga fördelar genom att profilera sig med en miljöanpassad tvättmetod.

Thesis (M.M.A.)--University of Washington, 1997. / Includes bibliographical references (leaves [168]-182).

Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 139-154).

Thesis (M.A.)--Simon Fraser University, 1999. / Includes bibliographical references (leaves 106-113).