No description available.

No description available.

Le travail décrit dans cette thèse couvre une étude fondamentale sur des canaux artificiels d'eau et sur des matériaux membranaires incorporant ces canaux. Structuré en quatre chapitres, la thèse commence par une présentation de l'état de l’art sur les systèmes biomimétiques de transport d'eau et des membranes biomimétiques. Au centre de tous ces travaux de recherche sont les protéines biologiques hautement efficaces et sélectives, les Aquaporines. Le deuxième chapitre présente les canaux artificiels d'eau à base d'imidazole-quartet. Les similitudes structurelles et fonctionnelles avec les Aquaporines sont discutées et caractérisées par plusieurs méthodes expérimentales. Les structures à l'état solide obtenues à partir de monocristaux présentent une organisation très similaire des I-quartets avec leurs homologues biologiques. Le biomimétisme fonctionnel du transport de l'eau a été démontré par des expériences cinétiques de transport à travers des systèmes vésiculaires. Le mécanisme de translocation de l'eau et l'organisation confinée dans des environnements lipidiques a été confirmé par des simulations dynamiques moléculaires, tandis que la preuve physique de l'eau orientée dipolaire dans les canaux intégrés aux lipides a été fournie par des expériences de spectroscopie IR polarisée. Le troisième chapitre présente de nouveaux canaux d'eau artificiels en utilisant une stratégie d'auto-assemblage. De nouveaux composés à base de diol, de tétrazacrown et de tryarilamine capables de transporter l'eau sont décrits. Le dernier chapitre décrits le passage du niveau moléculaire aux matériaux membranaires macroscopiques incorporant des canaux d'eau artificiels. Deux configuration membranaires différentes ont été décrites: des membranes en couche mince par l'incorporation de nanoparticules à base d'imidazole dans des polymères de polyamide et des membranes de la cellulose régénérée chimiquement greffée par des monomères de canaux d'eau artificiels. Les membranes ont été caractérisées par diverses méthodes d'imagerie et d'analyse et leurs performances ont été testées dans des expériences d'osmose inverse et de filtration d'osmose directe. La thèse est conclue avec une partie de conclusion générale, comprenant des perspectives pour les développements futurs. / The work described in this thesis covers an in depth fundamental study of artificial water channels and of membrane materials incorporating these channels. Structured in four chapters, the thesis begins with a presentation of the state of the art in the field of biomimetic systems and membranes for water transport. The center of the described research work is the family of highly efficient and selective biological water transporter proteins, the Aquaporins. The second chapter presents the description of imidazole-quartet supramolecular artificial water channels. Structural and functional similarities with Aquaporins are discussed and based on several experimental methods. Single-solid state structures present very similar organization of confined water wires as found in their biological counterparts. Functional mimicry of water transport has been proved through stopped flow experiments in vesicular systems. Further characterization concerning water translocation mechanism and confined organization in lipid environments have been obtained through molecular dynamic simulations, while physical evidence of dipolar oriented water in lipid embedded channels has been provided by sum frequency generation experiments. The third chapter presents novel artificial water channels. New diol, tetrazacrown and tryarilamine based compounds have been described, with a main focus on design, synthesis, self-assembly and water transport properties. The last chapter makes the transition from the molecular systems to macroscopic membrane materials incorporating artificial water channels. Two different approaches have been described: thin film nanocomposite membranes based on the incorporation of imidazole-quartet nanoparticles in polyamide polymers and chemically grafted regenerated cellulose membrane through the use of custom monomers for the obtaining of artificial water channels. The membranes have been characterized through various imaging and analytical methods and their performances have been tested in reverse and forward osmosis experiments. The thesis is concluded with a general conclusion part, including perspectives for future developments.

An experimental study of the effect of initial conditions on the development of Rayleigh Taylor Instabilities (RTI) at low Atwood numbers (order of 10-4) was performed in the water channel facility at TAMU. Initial conditions of the flow were generated using a controllable, highly reliable Servo motor. The uniqueness of the study is the system’s capability of generating the required initial conditions precisely as compared to the previous endeavors. Backlit photography was used for imaging and ensemble averaging of the images was performed to study mixing width characteristics in different regimes of evolution of Rayleigh-Taylor Instability (RTI). High-speed imaging of the flows was performed to provide insights into the growth of bubble and spikes in the linear and non-linear regime of instability development. RTI are observed in astrophysics, geophysics and in many instances in nature. The vital role of RTI in the feasibility and efficiency of the Inertial Confinement Fusion (ICF) experiment warrants a comprehensive study of the effect of mixing characteristics of RTI and its dependence on defining parameters. With this broader objective in perspective, the objectives of this present investigation were mainly threefold: First was the validation of the novel setup of the Water channel system. Towards this objective, validation of Servo motor, splitter plate thickness effects, density and temperature measurements and single-mode experiments were performed. The second objective was to study the mixing and growth characteristics of binary and multi-mode initial perturbations seeking an explanation of behavior of the resultant flow structures by performing the first ever set of such highly controlled experiments. The first-ever set of experiments with highly controlled multi-mode initial conditions was performed. The final objective of this study was to measure and compare the bubble and spike velocities with single-mode initial conditions with existing analytical models. The data derived from these experiments would qualitatively and quantitatively enhance the understanding of dependence of mixing width on parametric initial conditions. The knowledge would contribute towards a generalized theory for RTI mixing with specified dependence on various parameters, which has a wide range of applications. The system setup was validated to provide a reliable platform for the novel multi-modal experiments to be performed in the future. It was observed that the ensemble averaged mixing width of the binary system does not vary significantly with the phase-difference between the modes of a binary mode initial condition experiment, whereas it varies with the amplitudes of the component modes. In the exponential and non-linear regimes of evolution, growth rates of multi-mode perturbations were found to be higher than the component modes, whereas saturation growth rates correspond to the dominant wavelength. Quadratic saturation growth rate constants, alpha were found to be about 0.07 ± 0.01 for binary and multi modes whereas single-mode data measured alpha about 0.06 ± 0.01. High-speed imaging was performed to measure bubble and spike amplitudes to obtain velocities and growth rates. It was concluded that higher temporal and spatial resolution was required for accurate measurement. The knowledge gained from the above study will facilitate a better understanding of the physics underlying Rayleigh-Taylor instability. The results of this study will also help validating numerical models for simulation of this instability, thereby providing predictive capability for more complex configurations.

This thesis presents the experimental results on the formation of flow separation from a rigid wall with a salient edge. In the case of automotive vehicles or aircrafts with rear cargo compartment doors, such salient edges are at the origin of separated wake flows resulting in increased drag and other disturbing effects. Recent studies of Ahmed et al. (1984) on simplified geometries showed the strong influence of the slant angle on the flow separations. In this study, the geometry is further simplified to examine the flow separation under two-dimensional conditions. The experimental configuration consists of a fixed horizontal front panel and an attached rear panel with variable slant angle. The experiments were carried out in a low speed water channel to analyze the flow structure by flow visualization techniques. The hydrogen bubble technique nd PIV measurements are used to obtain both qualitative and quantitative information on the flow structure. The electrolytic precipitation technique is used to analyze the flow separation in more detail. The slant angle varied between 0 and 35 degrees while the Reynolds numbers of the model was fixed to 24800 and 50500. As a function of slant angle and Reynolds number, two different types of flow separation were observed: boundary layer separation due to adverse pressure gradient and the so called &ldquo / inertial separation&rdquo / at the edge singularity. Future strategies to control the formation of the wake flow highly depend on the very different flow structure of these two types of separation.

[pt] O estudo de bolhas de separação laminar em aerofólios que operam a baixos números de Reynolds é importante para diversas aplicações tais como: turbinas a gás, geradores eólicos, veículos aéreos não tripulados, dentre outros. A perda de performance desses equipamentos, normalmente, está associada à presença e à ruptura de bolhas de separação. Neste trabalho buscou-se investigar, experimentalmente, os efeitos relacionados a variação súbita no nível de perturbações do escoamento a montante da região de formação da bolha. A ideia é avaliar como o escoamento se comporta em uma situação onde o nível de turbulência do escoamento livre pode variar. No caso de turbinas, essa condição pode ser observada quando as esteiras dos aerofólios de um estágio influenciam aquelas do estágio seguinte. Apesar da relevância prática, o regime transiente foi pouco investigado na literatura. Para o presente estudo foi utilizado um canal de água do Laboratório de Engenharia de Fluidos. O gradiente de pressão, necessário para a formação da bolha, foi gerado com uma placa convergente-divergente e o nível de perturbações na camada limite foi controlado através de um gerador do tipo fita vibrante. Os campos de velocidades na região da bolha foram medidos com a técnica de Velocimetria por Imagem de Partícula com alta resolução temporal. A geração de perturbações e as medições de velocidade foram sincronizadas, permitindo assim o uso de técnicas de extração de médias de eventos. Caracterizou-se a bolha em um regime estacionário, onde não houve excitação de perturbações controladas a montante da bolha. Os resultados foram comparados com referências da literatura, apresentando boa concordância. No regime transiente, analisou-se o escoamento desde o instante em que o gerador de perturbações foi subitamente desligado até a recuperação completa da bolha. Nessas condições, notou-se que bolha cresceu até atingir um comprimento maior do que o observado no regime estacionário. Somente após o desprendimento de um grande vórtice, a bolha voltou a exibir características similares àquelas do regime estacionário. Esse comportamento se assemelha àquele reportado na literatura para ruptura de bolhas (bursting) mas que ainda não é bem compreendido. / [en] Laminar separation bubbles in Low Reynolds number airfoils is important for several applications such as: gas turbines, wind generators, unmanned aerial vehicles, among others. The performance loss of this airfoils is usually associated with the presence and rupture of separation bubbles. The effects related to the sudden variation of the level of flow disturbances upstream of the bubble formation region are investigated in this work. The idea is to evaluate how the flow behaves when subjected to a sudden variation in the freestream turbulence. This condition can be observed in airfoil cascade of turbines, where the wake of a previous stage can influence the next. Although this scenario is of practical relevance, it has been poorly investigated in literature. For the present study, a water channel from the Fluids Engineering Laboratory was used. The pressure gradient, necessary for the bubble formation, was generated with a convergentdivergent plate and the level of disturbances in the boundary layer was controlled using a vibrating ribbon. Velocity fields in the bubble region were measured using the Particle Image Velocimetry technique with high temporal resolution. The generation of disturbances and measurements were phase locked, thus allowing the use of ensemble average techniques. The bubble was characterized in a so called steady regime, where there was no excitation of controlled disturbances upstream of the bubble. The results were compared with references in the literature, showing good agreement. In the transient regime, the flow was analyzed from the moment the disturbance generator was suddenly turned off until the bubble had completely recovered. In these conditions, it was noted that the bubble grew until reaching a length greater than that observed in the stationary regime. Only after the release of a large vortex did the bubble return to exhibit characteristics similar to those of the stationary regime. This behavior is similar to that reported in the literature for bubble rupture (bursting) but that is still not well understood.

No description available.

No description available.

Le but principal du présent travail est ainsi de réaliser une expérience de mélange par la turbulence, dans laquelle il est possible de déterminer et de quantifier les temps de cohérence des différentes échelles spatiales des fluctuations du champ de vitesse et du champ de concentration qu'il transporte et mélange. La turbulence est ici voisine de la situation idéale statistiquement homogène et isotrope, et la configuration est conçue pour qu'il en soit de même pour le champ de concentration. La turbulence est créée par une grille placée perpendiculairement à un écoulement uniforme à l'extérieur des couches limites qui se développent le long des parois de la veine d'essais à section carrée constante. L'écoulement de la présente étude est un écoulement d'eau, dans lequel le champ de concentration est celui d'une solution de Rhodamine B injectée au niveau de la grille à travers des injecteurs équi-répartis le long des barreaux de celle-ci. Ce choix, dicté par la technique de mesure du champ de concentration par Fluorescence Induite par Laser, permet en outre de mesurer le champ de vitesse par une autre technique optique, elle aussi non-intrusive. Pour se rapprocher le plus de la théorie d'un mélange idéal statistiquement homogène et isotrope sans vitesse moyenne, on considère dans l'expérience, conformément à l'hypothèse de Taylor, que toutes les échelles associées à chacun de ces champs, sont convectés à la vitesse moyenne U de l'écoulement, et l'on suit une "boîte de turbulence" qui se déplace à cette vitesse le long de la veine. Par suite déterminer l'état de la turbulence en un point donné de cette boite à l'instant t et à l'instant t'=t+dt, revient à l'étudier dans l'expérience à t à l'abscisse x de la veine d'essai, et à t' à l'abscisse x+dx , avec dx=Udt, où se trouve le point de la boîte aux deux instants successifs. Les résultats expérimentaux concernant les échelles pour lesquelles l'isotropie statistique est satisfaite, permettent alors de vérifier une phénoménologie de l'évolution de la cohérence temporelle des diverses échelles spatiales du champ des fluctuations de concentration fondée sur les idées de Comte-Bellot et Corrsin. Cette expérience, est en outre l'occasion de donner des résultats sur les densités de probabilité de diverses propriétés statistiques des champs de fluctuation de vitesse. / The main purpose of this work is to make an experiment of mixing by turbulence, in which it is possible to determine and quantify the coherence time of the different spatial scales of fluctuations of a scalar field. We measure concentration fluctuations of rhodamine B by Planar Laser Induced Fluorescence (PLIF) which is transported and mixed by velocity fluctuations. These latter ones are generated by a grid placed perpendicularly to the flow in a water channel and are measured by Particle Image Velocimetry (PIV). The concentration field is injected in the flow by injectors regularly spaced on the grid so that it is a situation where both the velocity and the concentration fields are statistically homogeneous and isotropic. To get as close as the theory of statistically homogeneous and isotropic turbulence with no mean velocity, we consider, according to Taylor's hypothesis, that all scales associated with each of these fields are convected with the mean velocity U of the flow, and we follow a "turbulent box" that moves at U along the channel. As a result determining the state of turbulence at a given point of the box at time t and time t ' = t + dt, is like studying in the experiment at time t and space x of test section, and time t' and space x + dx of the test section, with dx = U dt. When statistical isotropy is satisfied, we can verify a phenomenology of the evolution of the temporal coherence of various space scales of the concentration fluctuation fields based on the ideas of Comte-Bellot and Corrsin. This experiment is also an opportunity to give results on probability densities of various statistical properties of fluctuating velocity fields.