U.S. Army Corps of Engineers (USACE) regulates fill of jurisdictional waters of the United States including wetlands. Recent USACE regulations set a threshold of impacts to wetlands at one-half acre. Impact area can be determined by Global Positioning System (GPS) measurement of wetland boundary and Geographic Information System (GIS) calculation of impact area. GPS point selection methods include (1) equal time interval, (2) transect and (3) intuition. Four two-acre shapes were measured with each GPS method and brought into GIS for area calculation. Analysis of variance and Root Mean Square Error analyses determine that the transect method is an inferior point selection method in terms of accuracy and efficiency.

Constructed wetlands are considered an inefficient technology for long-term phosphorus (P) removal. The P retention effectiveness of subsurface wetlands can be improved by using appropriate substrates. The objectives of this study were to: (i) use sorption isotherms to estimate the P sorption capacity of the two materials, masonry sand and light weight expanded shale; (ii) describe dissolved P removal in small (2.7 m3) subsurface flow wetlands; (iii) quantify the forms of P retained by the substrates in the pilot cells; and (iv) use resulting data to assess the technical and economic feasibility of the most promising system to remove P. The P sorption capacity of masonry sand and expanded shale, as determined with Langmuir isotherms, was 60 mg/kg and 971 mg/kg respectively. In the pilot cells receiving secondarily treated wastewater, cells containing expanded shale retained a greater proportion of the incoming P (50.8 percent) than cells containing masonry sand (14.5 percent). After a year of operation, samples were analyzed for total P (TP) and total inorganic P (TIP). Subsamples were fractionated into labile-P, Fe+Al-bound P, humic-P, Ca+Mg-bound P, and residual-P. Means and standard deviations of TP retained by the expanded shale and masonry sand were 349 + 169 and 11.9 + 18.6 mg/kg respectively. The largest forms of P retained by the expanded shale pilot cells were Fe+Al- bound P (108 mg/kg), followed by labile-P (46.7 mg/kg) and humic-P (39.8). Increases in the P forms of masonry sand were greatest in labile-P (7.5 mg/kg). The cost of an expanded shale wetland is within the range of costs conventional technologies for P removal. Accurate cost comparisons are dependent upon expansion capacity of the system under consideration. Materials with a high P sorption capacity also have potential for enhancing P removal in other constructed wetland applications such as stormwater wetlands and wetlands for treating agricultural runoff.

Nutrient availability, water depth, competition, and soil management effects on cattail (Typha latifolia) growth in wetland systems were examined. Soluble reactive phosphorous (SRP), nitrate-nitrogen (NO3-N), and ammonia-nitrogen (NH3-N) removals were tested at a constructed wetland receiving municipal wastewater effluent. Over all, no significant differences in nutrients occurred between diverse planted and cattail areas. T. latifolia seeds, under the canopy of Eleochoris macrostachya, had low seed germination. Established stands of emergent vegetation can prevent cattail colonization and spread. Germination of T. latifolia at various water depths was tested, and depth impacts on cattail seedling growth and survival were ascertained using various moist soil management techniques in three ponds. Water levels at 0cm and >40cm can adversely impact cattail establishment.

In this study we assessed growth laws of sub-delta crevasses in the Mississippi River delta plain, experimental laboratory deltas, and compared them to previously studied river dominated large deltas worldwide. Metrics for channel and delta geometry for each system were obtained using a combination of geospatial tools, bathymetric datasets, sediment size, and hydrodynamic observations. Most crevasses and experimental deltas appear to obey delta growth laws suggesting that they exhibit planform metrics similar to larger deltas. However, some channels within each system, exhibit outlier behavior (e.g. asymmetric growth) where channel length is much larger than channel width. Hydrodynamic observations and morphodynamic modeling results, support the role of confinement in governing this response, through direct lateral confinement of the receiving basin width and depth thus guiding channels, and indirect confinement caused by sediment cohesion, whereby natural levees guide the systems asymmetric channel growth.

Submitted in complete fulfillment for the degree of Doctor of Philosophy (Biotechnology) in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa, 2016. / Many households in developing countries are still without proper sanitation systems. The problems are even more prevalent in rural communities where there are no septic systems in place for the treatment of wastewater. This has resulted in the urgent need for the development and implementation of innovative wastewater treatment systems that are inexpensive, environmental friendly and are able to reduce contaminants to levels that pose no harm to the communities. Constructed rhizofiltration systems have been explored for this purpose. They have been used for many decades in many countries with varying degrees of success at the primary, secondary and tertiary levels of wastewater treatment. Poor optimization of this technology has been due to limited information available about the roles played by the whole system as well as by each component involved in the treatment technology. The current work elucidates the role played by macrophytes and microbial biofilms in the removal of nutrients in the rhizofiltration system. Factors affecting waste removal as well as environmental friendliness of the system were also investigated. The rhizofiltration system was constructed in Durban and was divided into planted (planted with Phragmites australis and Kyllinga nemoralis) and unplanted (reference) section. Dissolved oxygen (DO), pH, water temperature, total dissolved solids (TDS), electrical conductivity (EC) and salinity were monitored. The removal efficiency of nutrients was measured using spectrophotometric methods by measuring the concentration of ammonia, nitrate, nitrite, phosphate and orthophosphate in the wastewater pre- and post-treatment. The total organic carbon, chemical oxygen demand (COD), total Kehldjahl nitrogen, biological oxygen demand (BOD), ammonia, nitrate and the flow rate of wastewater into the system from the settling tank were used for the estimation of carbon dioxide, methane and nitrous oxide emitted from the rhizofilter using the 2009 EPA formulae. Both the planted and reference sections of the system removed nutrients with varying efficiencies. The reduction of nutrients in the rhizofilter was found to be seasonal, with most nutrients removed during the warm seasons. The system also retained more nutrients when wastewater containing low levels of nutrients was used. The unpaired t-test was used to determine the differences between nutrient removals between planted and reference sections. Higher reduction efficiencies of nutrients were obtained in the planted section. Up to 65% nitrite and 99% nitrate were removed while up to 86% total phosphorus was removed in a form of orthophosphate (86%). Removal of total nitrogen was shown to increase under high temperature conditions, while the same conditions decreased the total phosphorus removal. High temperatures also increased the performance of the system. The reduction of nutrients in the system corresponded to reduction of the chemical oxygen demand which also positively correlated to the dissolved oxygen concentration. Considering the discharge limits for all nutrients, the discharges in the effluent of the planted section were within the allowable limits as per South Africa’s Department of Water affairs and Forestry in 2012 but not in 2013. The results obtained in 2013 were due to increased nutrient loading introduced into the system. Diverse microbial communities occurred in the treatment system, with more diversity in the planted section. These organisms were supported by macrophytes in the planted section, and were responsible for nitrogen and phosphorus transformation. This explains why total nitrogen and phosphorus reduction was higher in the planted compared to the reference section. Both the planted and the reference sections of the rhizofiltration system produced the greenhouse gases. When the two sections were compared, the planted section produced more gases. Gases emitted by both sections were lower when compared to emission from sludge treatment reed beds and other conventional systems of wastewater treatments. These findings indicated that constructed rhizofiltration is a cleaner form of waste treatment, producing significantly less greenhouse gases and affecting less of a climate change. Findings of this work have revealed that rhizofiltration technology can be used as a low-cost alternative technology for the treatment of wastewater, using the combination of macrophytes and microbial biofilms. Macrophytes accumulated nitrogen and phosphorus as well as supported diverse microorganisms that metabolized and reduced nutrients in the rhizofiltration unit. / D

Tidal freshwater forest restoration after dam removal has been unexplored to date. This study elucidated pre-dam forest composition, as well as post-dam edaphic and microtopographical attributes and woody species recruiting along a narrow ecotone of a 29.3-ha tidal freshwater wetland. The ≈65-year-old historical forest (15 species, 200 stems ha-1) and ≈7-year-old contemporary forest (40 species and 11,009 stems ha-) community dominants were dissimilar (Fraxinus spp. vs. Liquidambar styraciflua, respectively). Pre-dam environmental conditions were unknown. Post-dam edaphic water content, organic matter, redox potential and microtopography differed significantly across tidal sites but were less variable in non-tidal sites. Shifts in the contemporary woody community composition and the concomitant increase in stem density and seedling:sapling ratios with elevation likely owed to significant changes in microtopography and edaphic attributes. Developing ecotones that contain variable microtopography may be extremely important for successful natural woody recruitment after dam removal from a tidal freshwater system.

Soils, and the microbial communities contained within them, are vital for most chemical, physical, and biological processes. This study investigated how microbial community structure responded to environmental changes, such as hydrology, across vertical space (depth) and time in an emergent fresh water wetland. Research was conducted in a non-tidal freshwater wetland along the James River (Charles City County, Virginia) by establishing plots in two areas that experienced different hydrologic regimes and plant communities. Soil cores (30 cm) were collected monthly from January 2008 to February 2009, and then every two to three months thereafter until October 2009, for a total of 17 sampling events. The soil cores were divided by depth (Top: 0 – 10 cm, Bottom: 20 – 30 cm) and analyzed for a variety of soil properties including: pH, organic matter (OM), water content (WC), C:N, redox, and root biomass. Additionally, above-ground plant communities were monitored during the growing seasons. Based on preliminary analysis, one date from each season (Winter, Spring, Summer, and Fall) from both sampling years were selected for in depth analysis of the microbial community structure via Terminal Restriction Fragment Length Polymorphism (T-RFLP) of 16S-rRNA. Analysis of variance (ANOVA) found significant differences were found between the environmental parameters in regards to site, depth, and season. Three physical-chemical variables (WC, OM, and redox) were different between sites, but the majority of environmental parameters were significantly different between depths and seasons. The dominant environmental effect on microbial communities was soil depth and, overall, no seasonal patterns were observed in the microbial communities. Further, archaeal communities were most strongly correlated to changes in water content, while redox was strongly correlated to changes across depth in the bacterial communities. Collectively, these results demonstrate that wetland microbial communities are not a product of one separate variable or spatial scale, but result from various factors interlinked to shape microbial communities. More long-term studies are needed to investigate interactions between microbial community structure and environmental variables in these dynamic ecosystems.

In this study the patterns of sediment transport and deposition in the channels and receiving basin of a crevasse splay in the modern Mississippi River delta are examined, with emphasis on the development of a distributary mouth bar. Simultaneous hydroacoustic and optical measurements on the mouth bar show that the bar conforms to the progradational stage of an existing conceptual model of mouth bar development. This is confirmed by cores dated using Beryllium-7, which provides a record of the deposition on the bar over a 90-day period. Stratigraphic data from cores obtained on the bar are used to extend the conceptual model to account for variable riverine inputs. A numerical model, developed and validated using field data is capable of representing the fundamental sedimentary processes responsible for mouth bar progradation. These results will be of interest to coastal geologists, engineers and coastal managers alike.

Climate change is a clear indication that humanity’s innate affiliation with nature has been suppressed, and that our detrimental anthropogenic activities on the earth’s natural resources and systems, have exceeded the earth’s ecological capacity to cope and regenerate. This threatens environmental sustainability, which subsequently has social and economic implications. Environmental conservation is humanity’s aim towards re-affiliating its deep rootedness in nature, in order to ensure a sustainable co-existence with other species and natural systems, and thus convince responsive lifestyles, which allows humanity to match natural-resource extraction to the rate at which the earth can regenerate. The role of environmental sustainable architecture, towards mitigating humanity’s impact on global warming, still leaves a disparity between human relationships and interactions with the natural environment. The notion of a phenomenology-guided design inquiry, an application of enhanced multi-sensory experiences is identified, to re-affiliate humanity with nature and to provoke a sense of urgency for greater protection of the natural environment, through an immersive experience of enhanced human-nature interactions with nature. Furthermore, this notion is applied in the programme of eco-tourism and qualitative ecological research; the proposed site choice; the concept design approach and technical resolution of the project. The research recognises the conservation of sensitive ecosystems such as the iSimangaliso Wetland Park in northern KwaZulu-Natal, as one of the core strategies for environmental sustainability, and its appropriateness as a site for immersed experiences with nature and the sharing of ecological knowledge for the benefit of the wider communities in South Africa and internationally. The research proposes an environmentally responsible and contextually appropriate architectural design, for a wetland, estuary and marine conservation centre in the iSimangaliso Wetland Park. Furthermore, the programme responds to the key contextual issues concerning the park by assisting with the ecological conservation and growth, as well as the social and economic sustainability of the iSimangaliso Wetland Park and surrounding communities. This is achieved by proposing an architectural programme which functions as an interface and mediator for the key issues concerning the park, that is of research, education, tourism and community participation, through which the most concerning issue of ecological conservation occurs.

Abstract____________________________________________________________________ 2 ABSTRACT: Zea mays is generally considered to be a plant with inducible lysigenous aerenchyma formation. The degradation of some cortical cells is triggered by environmental conditions, usually in the form of stress (submergence etc.). These cells die in a process that shows signs characteristic for programmed cell death, such as nuclear DNA fragmentation or apoptotic ultrastructural alterations. Aerenchyma formed in primary roots of thirteen examined maize accessions, irrespective of cultivation conditions. The aerenchyma fraction correlated with the root length, not with its age. The dependence of aerenchyma formation on the presence of this phytohormone was proved by using an inhibitor of ethylene synthesis (AOA). It was found out that the aerenchyma formation depended on light conditions and that the lysigenous intercellulars intercellular spaces colocalized with areas with cells with characteristically fragmented nuclear DNA (TUNEL-positive nuclei). In experiments using the TUNEL reaction it was necessary to determine new dilution of the enzymatic mixture for the examined plant material. Only the observation of surface planes of free-hand root sections was considered relevant in both TUNEL-TMR and TUNEL-AP assays. TUNEL-AP...