Orientador: Clarice Weis Arns / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-17T13:48:54Z (GMT). No. of bitstreams: 1 Felippe_PauloAnselmoNunes_D.pdf: 7878570 bytes, checksum: bacb59d6d40fdf4e282eb9e5e89306a9 (MD5) Previous issue date: 2011 / Resumo: O Brasil figura entre os maiores produtores e exportadores do mundo de carne de frango e ovos, o que confere a avicultura a capacidade de interferir na construção do PIB nacional. Estes índices envolvem a necessidade de uma alta produtividade e consequentemente um manejo zoosanitário suficiente para atender a economicidade do setor. Este estudo focou dois gêneros virais de importância na avicultura, o Coronavirus e o Metapneumovirus, objetivando verificar a presença de vírus em aves silvestres e sinantrópicas da avifauna brasileira, visando compreender melhor a eco-epizootiologia das respectivas morbidades de que são agentes etiológicos. Para tanto se coletou 134 amostras através de swabs traqueais e cloacais de 53 aves silvestres brasileiras de 20 espécies diferentes e de 14 pombas (Columba livia), que não possuíam sinais de doença. Além destas aves, foi também objeto de estudo amostras de galinhas e frangos comerciais oriundos de vários estados da federação nos anos de 2003 a 2009, com sintomas respiratórios. O material genético dos vírus encontrados foi extraído e submetido à reação de nested RT-PCR, destinado a amplificar a região hipervariável do gene que codifica a proteína S1 dos coronavírus e o gene que codifica a proteína G dos metapneumovírus. Os amplificados foram então sequenciados e analisados construindo-se árvores filogenéticas e matrizes de similaridade através de recursos de bioinformática. Foi possível recuperar seis (06) amostras de coronavírus de Columba livia e vinte e três (23) de galinhas domésticas. No estudo filogenético se observou dois grandes clusteres; um agrupando-se com o genótipo Massachusetts e outro com D207; das amostras de pomba 5 agruparam-se com Massachusetts; 1 amostra de galinha e uma de pomba agruparam com o genótipo Connecticut e 1 de galinha com o Arkansas. As amostras oriundas de pomba e as de galinha compartilharam uma similaridade que chegou a 100%, para algumas delas. Quanto aos metapneumovírus recuperou-se vírus de 7 aves silvestres, 7 de pombas e 15 de galinhas domésticas, sendo que estas amostras agruparam-se em dois grandes clusteres, um com o subtipo A (5 de aves silvestres, 2 de pombas e 1 de galinha) e outro com o subtipo B (2 de aves silvestres, 5 de pombas e 14 de galinha). A identidade genética entre os amplificados de galinha e de aves não domésticas chegou a 100%. Tanto para os coronavirus como para os metapneumovirus o isolamento de vírus com um de seus genes mais variáveis apresentando até 100% de identidade com os das galinhas domésticas, pode representar uma possível recuperação dos vírus atenuados utilizados nas vacinações de rotina, ou mesmo a passagem dos vírus patogênicos para estes animais, fazendo com que estes mantenham o agente no ambiente. Aves de diferentes espécies podem ainda funcionar enquanto um ambiente seletivo para estes vírus, provocando a fixação de novos genótipos virais, o que seria uma provável explicação para o fato destes agravos continuarem a ocorrer em granjas de todo o mundo em detrimento da existência de vacinas, além da possibilidade de surgimento de vírus recombinantes capazes de infectar humanos, como é o caso da SARS / Abstract: Avian Coronavirus and Metapneumovirus in Brazilian Domestic and Wild Birds: Molecular Characterization and Phylogenetic. Brazil is among the largest producers and exporters of poultry meat and eggs in the world, which gives the poultry industry the capacity to interfere in the construction of national GDB. These indexes involve the need of a high productivity and consequently a sufficient zoosanitary management for the economy of the sector. This study focused on two important viral genera for this, Coronavirus and the Metapneumovirus, to verify the presence of virions in wild birds and synanthropic ones of Brazil, seeking a better understanding of those diseases and your ecoepizootiology. For that, were collected one hundred thirty-four samples (tracheal and cloacal swabs) from fifty three Brazilian wild birds of twenty different species and fourteen from pigeons (Columba livia), without symptoms, and samples of chickens from several states of the federation in the years from 2003 to 2009, with swollen head syndrome. The virus genetic material found was subjected to the reaction of nested RT PCR, to amplify the hypervariable region of the gene encoding the coronavirus S1 subunit of the S protein and the gene encoding the G protein of metapneumovirus. The amplified were then sequenced and analyzed by constructing phylogenetic trees and similarity matrices using bioinformatics resources. Were isolated six (6) coronavirus from Columba livia and twenty three (23) from domestic fowl. In the phylogenetic analysis, two major clusters were observed one grouped with Massachusetts genotype and the other with D207; samples of five pigeons grouped with Massachusetts as well, one chicken sample and one from pigeon were grouped with genotype Connecticut and other from chicken with Arkansas genotype. The samples from pigeons and chickens shared a similarity which reached 100%. As for metapneumovirus, virus was recovered from seven wild birds, seven pigeons and fifteen from chickens. These samples were grouped into two major clusters; with subtype A (five from wild birds, two from pigeons and one from chicken) and with subtype B (two wild birds, 5 pigeons and 14 chickens). The genetic similarity between chicken amplicons to non domestic birds reached 100%. For both coronavirus and metapneumovirus, virus isolation from non domestic birds and their great similarity (reached 100%) with those from chickens, may represent a possible recovery of the attenuated virus used in routine vaccinations, or even the passage of pathogenic viruses for these animals, causing them to keep the agent in the environment. Birds of different species can also function as a selective environment for these viruses, leading to the establishment of new viral genotypes, which would be a likely explanation for the fact that these injuries continue to occur on farms around the world, even with the use of vaccines, besides the possibility of emergence of recombinant viruses capable of infecting humans, as is the case with SARS / Doutorado / Microbiologia / Mestre em Genética e Biologia Molecular

Polybrominated flame retardants (BFRs), organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were analysed in eggs of various wild bird species from industrialised areas in South Africa. Eggs were collected during the 2008 – 2009 breeding season, homogenised and sent to the Norwegian School of Veterinary Science (NVH) for gas chromatography-mass spectrometry (GC-MS) analysis. The concentration, contamination profile, and risk assessment were conducted for each pollutant class, while effects of species-specific variation, feeding guild, and feeding habitat were investigated. Levels of BFRs ranged between 2.6 – 44 ng g-1 wet mass (wm). The predominant congeners were BDE-153, -154, - 183 and -47. Results indicated species, in close contact to humans, had higher levels of BFRs, even at lower trophic levels. Therefore, diet was not the primary route of exposure. High concentrations and the occurrence of nona-PBDE congeners and HBCD indicated exposure to current use BFRs. There were measurable levels of OCPs and PCBs in all eggs analysed. Median OCP concentration ranged from 4.2 – 623 ng g-1 wm. DDE was the predominant compound in all species with the exception of the Crowned Lapwing (Vanellus coronatus) where chlordanes were predominant. This may indicate a species-specific attribute in the metabolic efficiency or diet of the genus, since these findings have been reported elsewhere in literature. Congener profiles indicated historic sources of lindane and DDT, while low levels of p,p’-DDT in al species indicate long-range or atmospheric transport. Even though levels of p,p’-DDE were approaching toxicological thresholds, no eggshell thinning was evident. Concentrations of OCPs and PCBs showed an increase with increasing tophic level. PCB concentrations ranged between 0.9 – 296.4 ng g-1 wm. When studying the metabolic potential of PCBs, metabolic groups showed good agreement with the biodegradability of the individual congeners. Phenobarbital-type (PB-type) inducer PCBs were prevalent, indicating the predominance of less toxic PCB congeners. However, non-ortho PCBs were not analysed. These congeners aslo could impact on the toxic potential of PCBs in wild bird eggs. Principle component analysis (PCA) indicated that variances within datasets could be attributed to congener profiles within species as they were affected by exposure, diet, position in the food web, and association with human activities. Although the individual groups of organohalogens were below no observed effect levels (NOELs), negative effects could occur through interactions of various compounds with each other, as well as the unique exposure profiles of South African bird populations. To assess the dietary exposure of low-income human populations living close to large industries, the occurrence of organohalogens was investigated in backyard chicken eggs. Levels of dioxins in these eggs were above the European Union (EU) recommended limits, whereas BFRs and OCPs levels were below levels of concern. Nevertheless, areas where DDT is actively applied to dwellings for malaria control should be urgently investigated. The presence of measureable levels of all the compounds considered, indicate an environment seriously impacted by anthropogenic activity that in the long term could negatively affect both the environment and human health, if it has not already done so. / Thesis (Ph.D. (Environmental Science))--North-West University, Potchefstroom Campus, 2011.

Polybrominated flame retardants (BFRs), organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were analysed in eggs of various wild bird species from industrialised areas in South Africa. Eggs were collected during the 2008 – 2009 breeding season, homogenised and sent to the Norwegian School of Veterinary Science (NVH) for gas chromatography-mass spectrometry (GC-MS) analysis. The concentration, contamination profile, and risk assessment were conducted for each pollutant class, while effects of species-specific variation, feeding guild, and feeding habitat were investigated. Levels of BFRs ranged between 2.6 – 44 ng g-1 wet mass (wm). The predominant congeners were BDE-153, -154, - 183 and -47. Results indicated species, in close contact to humans, had higher levels of BFRs, even at lower trophic levels. Therefore, diet was not the primary route of exposure. High concentrations and the occurrence of nona-PBDE congeners and HBCD indicated exposure to current use BFRs. There were measurable levels of OCPs and PCBs in all eggs analysed. Median OCP concentration ranged from 4.2 – 623 ng g-1 wm. DDE was the predominant compound in all species with the exception of the Crowned Lapwing (Vanellus coronatus) where chlordanes were predominant. This may indicate a species-specific attribute in the metabolic efficiency or diet of the genus, since these findings have been reported elsewhere in literature. Congener profiles indicated historic sources of lindane and DDT, while low levels of p,p’-DDT in al species indicate long-range or atmospheric transport. Even though levels of p,p’-DDE were approaching toxicological thresholds, no eggshell thinning was evident. Concentrations of OCPs and PCBs showed an increase with increasing tophic level. PCB concentrations ranged between 0.9 – 296.4 ng g-1 wm. When studying the metabolic potential of PCBs, metabolic groups showed good agreement with the biodegradability of the individual congeners. Phenobarbital-type (PB-type) inducer PCBs were prevalent, indicating the predominance of less toxic PCB congeners. However, non-ortho PCBs were not analysed. These congeners aslo could impact on the toxic potential of PCBs in wild bird eggs. Principle component analysis (PCA) indicated that variances within datasets could be attributed to congener profiles within species as they were affected by exposure, diet, position in the food web, and association with human activities. Although the individual groups of organohalogens were below no observed effect levels (NOELs), negative effects could occur through interactions of various compounds with each other, as well as the unique exposure profiles of South African bird populations. To assess the dietary exposure of low-income human populations living close to large industries, the occurrence of organohalogens was investigated in backyard chicken eggs. Levels of dioxins in these eggs were above the European Union (EU) recommended limits, whereas BFRs and OCPs levels were below levels of concern. Nevertheless, areas where DDT is actively applied to dwellings for malaria control should be urgently investigated. The presence of measureable levels of all the compounds considered, indicate an environment seriously impacted by anthropogenic activity that in the long term could negatively affect both the environment and human health, if it has not already done so. / Thesis (Ph.D. (Environmental Science))--North-West University, Potchefstroom Campus, 2011.

Made available in DSpace on 2014-06-11T19:26:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-10-20Bitstream added on 2014-06-13T20:33:45Z : No. of bitstreams: 1 prando_l_me_jabo.pdf: 407609 bytes, checksum: 947cac68a18e2787e6a5c7039ce51656 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / As características comportamentais de uma espécie em processo de domesticação são muito importantes, pois a criação requer animais dóceis e sociáveis. Assim, torna-se necessário conhecer essas características, pois têm consequências relevantes no bem estar e desempenho das aves em cativeiro. O comportamento de reintegração social (CRS) pode ser avaliado em um labirinto de campo aberto observando-se sua aproximação ou não do animal da mesma espécie, no período de cinco minutos. Dessa forma, o objetivo desse trabalho foi avaliar o CRS em perdizes determinando-se as fontes de variação e os parâmetros genéticos para essa característica em animais criados em cativeiro. Foi realizada estatística multivariada, utilizando-se análise de fatores que revelou a existência de dois grupos distintos dentro da amostra analisada. Os componentes de (co) variância foram obtidos por inferência bayesiana usando máxima verossimilhança para as características do CRS, para dados não transformados e transformados. As características estudadas, sendo que as duas primeiras representam o grupo dos evitadores e as duas últimas, o grupo dos locomotores, foram parado no quadrante 1 (Pq1), parado no quadrante 4 (Pq4), andando no quadrante 1 (Aq1) e andando no quadrante intermediário (Aqi). As herdabilidades estimadas para as características Pq1, Pq4, Aq1 e Aqi foram respectivamente, 0,28; 0,43; 0,19 e 0,27 para os dados transformados. Assim, concluise que a seleção pode ser eficiente para alterar as médias destas características / Behavioral traits of a species in the process of domestication are very important, because raising the animal in captivity requires docility and sociability. Therefore, it is necessary to know these traits, because they have relevant consequences for the well-being and the performance of captive birds. The behavior of social reinstatement (CRS) can be evaluated in an open field maze by observing if the bird approachs or not to a conspecific, during five minutes. Thus, the aim of this study was to evaluate the CRS of red-winged Tinamou in order to determine the sources of variation and the genetic parameters of this trait in animals raised in captivity. We performed multivariate analysis, using factor analysis and it was revealed the existence of two distinct groups within the sample the movers and the avoiders. The (co) variances estimates were obtained by bayesian inference using the maximum likelihood, for transformed and non-transformed data. The studies traits were standing in quadrant 1 (Pq1), standing in quadrant 4 (Pq4), displacement behavior the quadrant 1 (Aq1) and displacement behavior in the intermediate quadrant (Aqi), where the first two represent the group of avoiders and the last two, the group of movers. The heritability means estimates for the traits Pq1, Pq4, Aq1 and Aqi were respectively 0.28, 0.43, 0.19 and 0.27 for the transformed data. Thus, we concluded that selection could be effective to change the means of these traits

Orientador: Sandra Aidar de Queiroz / Banca: Danísio Prado Munari / Banca: Patrícia Tholon / Resumo: As características comportamentais de uma espécie em processo de domesticação são muito importantes, pois a criação requer animais dóceis e sociáveis. Assim, torna-se necessário conhecer essas características, pois têm consequências relevantes no bem estar e desempenho das aves em cativeiro. O comportamento de reintegração social (CRS) pode ser avaliado em um labirinto de campo aberto observando-se sua aproximação ou não do animal da mesma espécie, no período de cinco minutos. Dessa forma, o objetivo desse trabalho foi avaliar o CRS em perdizes determinando-se as fontes de variação e os parâmetros genéticos para essa característica em animais criados em cativeiro. Foi realizada estatística multivariada, utilizando-se análise de fatores que revelou a existência de dois grupos distintos dentro da amostra analisada. Os componentes de (co) variância foram obtidos por inferência bayesiana usando máxima verossimilhança para as características do CRS, para dados não transformados e transformados. As características estudadas, sendo que as duas primeiras representam o grupo dos evitadores e as duas últimas, o grupo dos locomotores, foram parado no quadrante 1 (Pq1), parado no quadrante 4 (Pq4), andando no quadrante 1 (Aq1) e andando no quadrante intermediário (Aqi). As herdabilidades estimadas para as características Pq1, Pq4, Aq1 e Aqi foram respectivamente, 0,28; 0,43; 0,19 e 0,27 para os dados transformados. Assim, concluise que a seleção pode ser eficiente para alterar as médias destas características / Abstract: Behavioral traits of a species in the process of domestication are very important, because raising the animal in captivity requires docility and sociability. Therefore, it is necessary to know these traits, because they have relevant consequences for the well-being and the performance of captive birds. The behavior of social reinstatement (CRS) can be evaluated in an open field maze by observing if the bird approachs or not to a conspecific, during five minutes. Thus, the aim of this study was to evaluate the CRS of red-winged Tinamou in order to determine the sources of variation and the genetic parameters of this trait in animals raised in captivity. We performed multivariate analysis, using factor analysis and it was revealed the existence of two distinct groups within the sample the movers and the avoiders. The (co) variances estimates were obtained by bayesian inference using the maximum likelihood, for transformed and non-transformed data. The studies traits were standing in quadrant 1 (Pq1), standing in quadrant 4 (Pq4), displacement behavior the quadrant 1 (Aq1) and displacement behavior in the intermediate quadrant (Aqi), where the first two represent the group of avoiders and the last two, the group of movers. The heritability means estimates for the traits Pq1, Pq4, Aq1 and Aqi were respectively 0.28, 0.43, 0.19 and 0.27 for the transformed data. Thus, we concluded that selection could be effective to change the means of these traits / Mestre

No description available.

Legal regulation of protection of wild bird species Abstract This thesis deals with legal regulation of protection of wild bird species at the international, European and Czech level. The aim of the thesis was to provide an overview of legal regulation of protection of wild bird species, and to evaluate whether this legal regulation is an effective means of achieving objectives which led to its adoption. The thesis consists of an introduction, three main parts and a conclusion. The first part of the thesis describes protection of birds in international law. In this part of the thesis there are first introduced international organizations important for protection of birds, in particular International Union for Conservation of Nature and BirdLife International, and then in the author's opinion the most relevant international conventions to protection of birds, namely the Ramsar Convention, the Bonn Convention, the Bern Convention and the Convention on Biological Diversity. The following second part of the thesis focuses on protection of birds in European law. In this part of the thesis legal regulation of protection of wild bird species in Directive 2009/147/EC (the Birds Directive) is described in detail including the related case law of Court of Justice of the European Union. The author pays attention...

Orientador: Adriane Pinto Wasko / Resumo: O Brasil apresenta umas das maiores diversidades de avifauna do mundo, sendo estimada a ocorrência de 1.919 espécies distribuídas em todo seu território. Entretanto, a destruição de habitats, poluição e captura excessiva, muitas vezes associadas ao comércio ilegal, têm levado a um declínio no número de indivíduos desse grupo animal. Uma das formas de combater esse tipo de crime ambiental e de reverter ou minimizar seus efeitos concerne à implementação de um banco de dados forenses com registros de ocorrências e informações biológicas sobre os animais apreendidos e desenvolvimento de ações de conscientização da população sobre esta problemática. Desta forma, o presente trabalho teve como objetivo desenvolver estratégias multidisciplinares para a proteção jurídica à conservação biológica de aves oriundas do tráfico de animais. Amostras de DNA de aves comercializadas ilegalmente e encaminhadas ao Centro de Medicina e Pesquisa em Animais Silvestres (CEMPAS), mantido pela Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista, foram utilizadas para geração de perfis genéticos sexo-específicos, por meio da amplificação de segmentos dos genes CHD-Z e CHD-W (Chromo Helicase DNA Binding). Adicionalmente, perfis de duas subespéciesde papagaio-verdadeiro (Amazona aestiva aestiva e Amazona aestiva xanthopteryx foram gerados por meio da amplificação e sequenciamento nucleotídico de segmentos dos genes mitocondriais citocromo C oxidase subunidade I (COI) e citocrom... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Brazil has one of the largest avifauna diversity in the world, with estimated 1,919 species distributed throughout its territory. However, habitat destruction, pollution and over-harvesting, often associated with illegal trade, have led to a decline in the number of individuals of this animal group. One possible approach to combat this type of environmental crime and to reverse or minimize its effects concerns the implementation of a forensic database with records of occurrences and biological information about the captured animals and the development of actions to raise population awareness about this problem. Therefore, the present work aimed to develop multidisciplinary strategies for the legal protection to the biological conservation of birds originated from the animal traffic. DNA samples of illegally traded birds, that were sent to the Center for Medicine and Research in Wild Animals (CEMPAS) maintained by the Faculty of Veterinary Medicine and Animal Science of the São Paulo State University, were used to generate sex-specific genetic profiles, through the amplification of segments of the CHD-Z and CHD-W (Chromo Helicase DNA Binding) genes. In addition, profiles of two subspecies of the Blue-fronted Amazon (Amazona aestiva aestiva and Amazona aestiva xanthopteryx) were generated, through amplification and nucleotide sequencing of segments of the mitochondrial genes cytochrome C oxidase subunit I (COI) and cytochrome b (CYB) and a segment of the 5S ribosomal DNA gene (... (Complete abstract click electronic access below) / Doutor

Submitted by BIANCA PICADO GONÇALVES null (bi_picado@hotmail.com) on 2018-03-27T19:06:54Z No. of bitstreams: 1 Tese_versão final 1.pdf: 3691717 bytes, checksum: 54912fed5f6b404afadb8be90dd16bf8 (MD5) / Approved for entry into archive by Luciana Pizzani null (luciana@btu.unesp.br) on 2018-03-28T15:29:07Z (GMT) No. of bitstreams: 1 gonçalves_bp_dr_bot.pdf: 3691717 bytes, checksum: 54912fed5f6b404afadb8be90dd16bf8 (MD5) / Made available in DSpace on 2018-03-28T15:29:07Z (GMT). No. of bitstreams: 1 gonçalves_bp_dr_bot.pdf: 3691717 bytes, checksum: 54912fed5f6b404afadb8be90dd16bf8 (MD5) Previous issue date: 2018-03-02 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O Brasil apresenta umas das maiores diversidades de avifauna do mundo, sendo estimada a ocorrência de 1.919 espécies distribuídas em todo seu território. Entretanto, a destruição de habitats, poluição e captura excessiva, muitas vezes associadas ao comércio ilegal, têm levado a um declínio no número de indivíduos desse grupo animal. Uma das formas de combater esse tipo de crime ambiental e de reverter ou minimizar seus efeitos concerne à implementação de um banco de dados forenses com registros de ocorrências e informações biológicas sobre os animais apreendidos e desenvolvimento de ações de conscientização da população sobre esta problemática. Desta forma, o presente trabalho teve como objetivo desenvolver estratégias multidisciplinares para a proteção jurídica à conservação biológica de aves oriundas do tráfico de animais. Amostras de DNA de aves comercializadas ilegalmente e encaminhadas ao Centro de Medicina e Pesquisa em Animais Silvestres (CEMPAS), mantido pela Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista, foram utilizadas para geração de perfis genéticos sexo-específicos, por meio da amplificação de segmentos dos genes CHD-Z e CHD-W (Chromo Helicase DNA Binding). Adicionalmente, perfis de duas subespéciesde papagaio-verdadeiro (Amazona aestiva aestiva e Amazona aestiva xanthopteryx foram gerados por meio da amplificação e sequenciamento nucleotídico de segmentos dos genes mitocondriais citocromo C oxidase subunidade I (COI) e citocromo b (CYB) e de um segmento do gene de DNA ribossomal 5S (DNAr 5S), a partir de amostras de animais mantidos no CEMPAS. A sexagem molecular, em exemplares de aves de diferentes famílias, permitiu a identificação de um ou de dois fragmentos de DNA em machos e fêmeas, respectivamente. Entre os marcadores moleculares utilizados para caracterizar Amazona aestiva, apenas o citocromo b evidenciou diferenças nucleotídicas entre as duas subespécies. Os dados dos perfis genéticos foram utilizados para implementação de uma plataforma online denominada de Forensic Bird Base, com acesso para fins de pesquisa e criminalística. Como produtos adicionais desse trabalho, foram gerados uma cartilha educativa visando popularizar a aplicação das Ciências Forenses no combate ao tráfico de aves silvestres e um manual jurídico para profissionais das áreas de biológicas, visando demonstrar as normas e leis vigentes no país acerca dos direitos dos animais, e as condutas que podem ser tipificadas ou não como crime contra a fauna. Os resultados e produtos gerados podem servir de subsídio para o delineamento de programas de manutenção e/ou reprodução de espécies de aves em cativeiro, elaboração de estratégias de reintrodução de exemplares em ambiente natural e para combater ou minimizar o tráfico ilegal de animais e seus efeitos, / Brazil has one of the largest avifauna diversity in the world, with estimated 1,919 species distributed throughout its territory. However, habitat destruction, pollution and over-harvesting, often associated with illegal trade, have led to a decline in the number of individuals of this animal group. One possible approach to combat this type of environmental crime and to reverse or minimize its effects concerns the implementation of a forensic database with records of occurrences and biological information about the captured animals and the development of actions to raise population awareness about this problem. Therefore, the present work aimed to develop multidisciplinary strategies for the legal protection to the biological conservation of birds originated from the animal traffic. DNA samples of illegally traded birds, that were sent to the Center for Medicine and Research in Wild Animals (CEMPAS) maintained by the Faculty of Veterinary Medicine and Animal Science of the São Paulo State University, were used to generate sex-specific genetic profiles, through the amplification of segments of the CHD-Z and CHD-W (Chromo Helicase DNA Binding) genes. In addition, profiles of two subspecies of the Blue-fronted Amazon (Amazona aestiva aestiva and Amazona aestiva xanthopteryx) were generated, through amplification and nucleotide sequencing of segments of the mitochondrial genes cytochrome C oxidase subunit I (COI) and cytochrome b (CYB) and a segment of the 5S ribosomal DNA gene ((DNAr 5S), from animal samples kept at CEMPAS. Molecular sexing, in different Family birds, allowed the identification of one or two fragments of DNA in males and females, respectively. Among the molecular markers used to characterize Amazona aestiva, only cytochrome b evidenced nucleotide differences between the two subspecies. Genetic profile data were used to implement an online platform called Forensic Bird Base, with access for research and criminalistic purposes. As an additional product of this work, an educational booklet was created, to popularize the application of Forensic Sciences against the traffic of wild birds, and a legal manual for professionals of the biological areas, aiming to demonstrate the norms and laws in force in the country regarding animals rights, and conducts that may be criminalized as a crime against wildlife. The generated results and products can be used as a subsidy to the design of programs for the maintenance and/or reproduction of bird species in captivity, the elaboration of strategies to reintroduce specimens in natural environments and to combat or minimize animals´s illegal trade and its consequences.