Wines were made from Pinot Noir and White Riesling grapes which had received field application rates of 2 and 4 lb./acre of the experimental bird repellent Mesurol (methiocarb or 3, 5 dimethyl- 4(methylthio)phenylmethylcarbamate). In addition, Pinot Noir must and press juice and White Riesling press juice were fortified with 15 ppm Mesurol (active ingredient) and vinified. Mesurol and its sulfoxide and sulfone metabolites were determined by gas chromstographic analysis as mesylate derivatives following fractionation on Florisil. Residues were also oxidized to and expressed as total sulfone. Mesurol residues, methiocarb and total sulfone, in field treated grapes sprayed with 4 lb./acre Mesurol exceeded the 15 ppm temporary tolerance established by the Environmental Protection Agency. Methiocarb residues in pomace, calculated on a dry weight basis, obtained from grapes harvested one day after the final application of Mesurol were in excess of the 75 ppm EPA temporary tolerance. Approximately 40 to 51% of the methiocarb residues measured in field, treated grapes remained associated with the pomace after pressing. Finished Pinot Noir and White Riesling wine, prior to bottling, contained in the order of 3-8 ppm representing 34 to 49% and. 13%, respectively, of the methiocarb residues originally present in grapes. The fate of Mesurol sulfide, sulfoxide and sulfone residues during the processing of Mesurol fortified White Reisling press juice was investigated. Settling of press juice was found to reduce methiocarb residues. Oxidation during vinification was minimal. Mesurol sulfide, sulfoxide, and sulfone residues in finished wine remained unchanged after 12 months of storage at cellar temperatures. Trace amounts of Mesurol sulfide phenol were detected with Mass Spectrometry. Volclay bentonite (KWK) fining at 4 and 8 lb./1000 gal. and membrane filtration (0.45 μ) were not effective in reducing Mesurol residues in wine. Reference-difference tests were used to evaluate the effect of Mesurol on finished wine sensory qualities. The sensory properties of wines made from grapes harvested seven days after the last application of Mesurol and Mesurol Fortified must and juices were not affected. Also, the chemical composition of these wines was not altered. Lag time and fermentation rate of Mesurol Fortified. Pinot Noir must and juice processed into wine were not affected. The course of fermentation in the presence of Mesurol was also monitored by recording mass loss in model studies. A slight inhibition in fermentation rate was observed, at levels below 25 ppm while a stimulation was evident at concentrations of up to 500 ppm. / Graduation date: 1981

An objective method to blend wines for standardizing flavor quality was developed. Aroma volatiles of varietal and white stock wines were analyzed at 6°C and 37°C by headspace gas chromatography with cryofocussing. Pattern similarity constants of the chromatographic profiles were entered into the simplex optimization program which determined the best blending ratios of wines to simulate the target wine. Thirteen and 23 vertices were required to give the optimum response for trials 1 and 2, respectively. For both trials the computer optimized blends could not be differentiated from the target wines by a sensory taste panel consisting of both untrained and expert judges. / Land and Food Systems, Faculty of / Graduate

Low nitrogen Chardonnay juice from an Oregon winery was fermented with a series of nutrient supplements, including diammonium phosphate (DAP), Fermaid K, Superfood, yeast extract, yeast hulls and thiamine. These treatments were evaluated for their contribution to the yeast assimilable nitrogen content (YANC) of the juice, which consists of the ammonium ion and α-amino acid content by NOPA (nitrogen by ophthaldialdehyde). The fermentation characteristics and the nutritional and chemical status of the finished wines were compared. Diammonium phosphate added 25 mg N/L from ammonia for every pound/1000 gallons (12.5 g/hL) used. Commercial yeast nutrient preparations were found to contribute around 10 mg N/L from ammonium ion per pound/1000 gallons (12.5 g/hL). NOPA and HPLC analysis of Superfood and Fermaid K indicated that they contributed only a small amount of amino acids. NOPA analysis of PL-50 yeast extract indicated that it may have added some nitrogen from amino acids. Nitrogenous compounds were taken up within the first few days of fermentation. Ammonium ion was depleted very quickly and did not reappear later in fermentation. Alpha-amino acids reached a low within approximately ten days of fermentation, then leveled out or increased towards the end of fermentation. Treatments containing high amounts of ammonia were observed to produce the most vigorous fermentations. These treatments were found to have the highest apparent levels of amino acids at the end of fermentation. The Control treatment, which had the lowest YANC content, appeared to utilize proline during fermentation. Sensory analysis was not conducted on the finished wines due to replication differences in reduced sulfur aroma found in preliminary screening. GC-MS analysis showed that the higher nitrogen treatments had levels of reduced sulfide compounds that were above sensory threshold, whereas moderate nitrogen treatments did not. Ethyl carbamate was not found in any of the finished wines. These data suggest that moderate levels of supplementation had a positive effect on both fermentation and finished wine quality. Higher levels of nutrient addition were effective at increasing fermentation rates but negatively affected the sulfide profile of finished wines. / Graduation date: 2001

Color stability and mouth feel quality are two of the most important aspects of red wine quality. Anthocyanins and proanthocyanidins are responsible for these attributes and it has been shown that weather conditions during the growing season and grape maturity can effect these components in wine. However, investigations into proanthocyanidin development are for the most part incomplete. Although it is known that weather affects vine metabolism, it has not clearly understood how phenolics are affected by temperature and heat summation. It is generally believed that the wines made with riper grapes improve in flavor and mouth feel as a result of an "improvement" in skin tannin "ripeness". The idea of "tannin ripeness" is usually used in the wine industry to explain this phenomenon, however, no scientific explanation for this concept has been given. The objective of this project was threefold: I) Monitor phenolic development in Pinot noir grapes over three consecutive growing seasons and determine how anthocyanin and proanthocyanidin development in grapes was affected by heat summation, II) investigate the transfer of grape phenolics into wine during fermentation and maceration and III) understand how grape maturity affected wine composition with a specific focus on proanthocyanidin structure. In this study, anthocyanin and proanthocyanidin development in Vitis vinifera L. cv. Pinot noir grapes (Pommard clone) were monitored for three consecutive vintages (2001-2003). Five cluster samples (x5 replicates) were collected for analysis each week beginning approximately 4 weeks prior to veraison and continued through commercial harvest. Weather information (temperature and heat summation) showed that the growing seasons became increasingly warmer from 2001 to 2003. By harvest time, 2003 had the highest concentration of proanthocyanidins in seeds (per berry weight) in comparison with the other two vintages. Similarly, proanthocyanidins in skins had the highest concentration in 2003 (per berry weight). However, there was not difference in the concentration of flavan-3-ol monomers in seeds (per berry weight) between the three vintages. Anthocyanins were not significantly different over the three vintages. There was some relationship between the concentration of some proanthocyanidin components in grape seeds and fresh seed weight. Information of grape and wine phenolics was compared with each year's temperature. The results suggested that changes in temperature and heat summation between vintages are associated with changes in proanthocyanidin content in grapes and wine. The data indicates that it is possible to predict proanthocyanidins in wine based upon early grape analysis. However, the anthocyanin content in grapes did not correlate with either weather or the anthocyanin content in wine. The concentration of seed and skin proanthocyanidins in grapes were compared with the proportions of seed and skin proanthocyanidins found in wine. Based upon proanthocyanidin extraction from seeds and skin during winemaking, a formula to predict proanthocyanidin content in wine based upon grape analysis at harvest and veraison was developed. From this formula, Pinot noir wine contained 7.8% of the proanthocyanidins from seed and 19% of the proanthocyanidins from skin analyzed from grapes at harvest, and 3.6% of the seed proanthocyanidin and 9.7% of the skin proanthocyanidin present in grapes analyzed at veraison. Based upon the analyses of this study, coupled with several informal sensory studies conducted on wine, the results of this thesis do not support the notion that "tannin ripeness" is due to structural changes in proanthocyanidin that occur during fruit ripening. Furthermore, this thesis suggests that "tannin ripeness" is not due to differential extraction of seed and skin proanthocyanidins as a result of fruit ripening. / Graduation date: 2005

The overall purpose of this study was to develop sensory evaluation methodology whereby wine quality can be predicted from juice quality. Descriptive analysis was used by a trained panel to describe Pinot noir juice and wine from three sources. From each source, one wine and four juice samples (a control and three treatments) were prepared. The following three treatments were applied to the crushed grapes prior to pressing the juice: freezing and thawing; skin contact with 250 ppm Pectinol VR (a pectinase); and skin contact with 250 ppm Rohapect D5L (another pectinase). The trained panel developed descriptive terminology which differed between Pinot noir juice and Pinot noir wine. The juice treatments created subtle, if any, aroma differences. Treatment differences were evident in color, as measured by human perception and by instrumental measurement. Few characteristics of wine aroma and/or color correlated with juice aroma and/or color. Perceived color correlated well with Somer's color density measurement (the sum of the corrected absorbances at 420 and 520 nm.). Some of the same samples were evaluated by a wine industry panel. They appeared to disagree regarding the definition of varietal character. Further research utilizing grapes from many sources is necessary to determine whether Pinot noir wine quality can be predicted from Pinot noir juice quality. / Graduation date: 1987

Thesis (MTech (Food Technology))--Cape Peninsula University of Technology, 2018. / Port is a wine style that comes from Portugal. It is a sweet fortified dessert wine that is made in red and white styles. The taste is a balanced and complex combination of berry fruit, acidity, sweetness, alcohol and tannins. The taste should be sweet, smooth, complex, with some spiciness and a dry finish, but not astringent (Anon., 2009). There are a variety of port types in terms of flavour intensity, aroma and sweetness levels. Young immature ports can be fruity, simple, coarse, spicy and astringent. The sweetness results from the natural grape sugar in the wine, while wine spirits is added to fortify and ensure microbiological stability during aging (Anon., 2009). In this study the work was done on the ruby port style wine, ruby port wine is well known for its characteristic of being bright red in colour and therefore also very difficult to preserve in terms of colour stability in general. Colour is one of the principle parameters of the quality of not only port wine but also red wine in general, since it is the first characteristic to be perceived by the consumer in the glass. The colour of port wine also gives an indication of possible defects, the body, age and the evolution of the wine during storage. Colour, therefore, has an important influence on the overall acceptability of the product to the consumer. During aging, the wine colour changes, mainly due to progressive structural changes of anthocyanins. These changes are often perceived as undesirable by port consumers. As a result, the Cape Port Producers Association (CAPPA) requested this type of research to be done on port wine to improve the port wine making process in order to also give port wine a more stable colour. Therefore the objective of this study was to manipulate some of the parameters in port wine making, such as type of spirit used to fortify, storage temperature and also storage time in order to improve optimum stability of port wine colour. From the first part of the study it was evident that the type of fortifying spirits, storage time and temperature had a significant effect on the colour of the port wine samples. The 96.5% (v.v-1) fortifying spirits, shorter storage time and storage temperature below 25oC resulted in a more stable ruby port colour as well as the lowest change over time. As the study progressed the design variables differed in terms of two types of cultivars used the spirits used to fortify the port wine samples with, addition of a pectolytic enzyme to some of the port wine samples, as well as storage time of 12 months and only two storage temperatures. It could be concluded that at the end of this part of the study, that port wine colour stability was affected by the interaction of the design variables in each treatment and less so by individual design variables in the study. It could also be concluded in this study that higher levels of acetaldehyde present in the spirits used to fortify port wine, did have a significant impact on ruby port wine and colour stability. The application of pectolytic enzyme preparation does not necessarily have a significant effect on its own but depends on the type of cultivar used. Storage time and temperature should also be kept to a minimum to ensure the desirable bright red colour of a ruby port wine.

The determination of wine authenticity and the detection of adulteration are attracting an increasing amount of attention for wine producers, researchers and consumers. Wine authentication and classification based on geographical origin has been widely studied. Most of these studies have achieved successful classification results. However, these studies have involved complicated and expensive procedures. Visible and near infrared spectroscopy (Vis-NIR) is recognized as a rapid and non-destructive technique. In recent years, several studies have been conducted using Vis-NIR spectroscopy to analyze wine for both quantitative and qualitative purposes. The aim of this research was to investigate the geographical classification of wines using Vis-NIR spectroscopy. The effect of temperature and measurement mode (transmission and transflectance) on Vis-NIR spectra was investigated to identify optimal conditions for wine sample analysis. It was found the optimal temperature is between 30 to 35°C and the shorter pathlength measurement condition has better prediction ability. Classification by geographical origin using Vis-NIR spectroscopy was investigated for sixty-three Tempranillo wines from Spain and Australia, and fifty Riesling wines from Australia, New Zealand and Europe. Discriminant partial least square regression (DPLS) and linear discriminant analysis (LDA) based on PCA scores were used to perform classification. Over 90% of the Tempranillo wines were correctly classified according to their geographical region using both DPLS and LDA. A classification rate of 72% was achieved for the Riesling wines. Vis-NIR technique provides a similar degree of reliability on wine classification comparable to those obtained using chemical composition. The results of this study demonstrate potential for Vis-NIR spectroscopy combined with multivariate analysis as a rapid method for classifying wines by geographical origin. / Thesis (M.Eng.Sc.)--School of Chemical Engineering, 2006.

Bibliography: p. 331-340. / x, 340 p. : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Using sensory ranking, studies aroma variability of a chardonnay and a cabernet sauvignon matured in new oak barrels. Oak wood-derived volatile compounds are analysed using gas chromatography, to suggest which natural and cultural variables are involved in each aroma variation. / Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 1998

Thesis (PhD)--Stellenbosch University, 2004. / ENGLISH ABSTRACT: The present study deals in the first instance with the improvement of current analytical techniques for the analysis of the non-volatile content of wines. An improved sample preparation method, using solid phase extraction (SPE), was initially developed for the analysis of organic acids, sugars and phenolic compounds. Consequently, modem analytical methodologies were assessed to obtain optimal techniques for the separation of various non-volatile compounds. A capillary electrophoresis (CE) method, demonstrably more reliable than currently used high performance liquid chromatography (HPLC) and CE methods, is proposed for the analysis of organic acids. HPLC with refractive index (RI) or evaporative light scattering detection (ELSD) proved more suitable than CE for the analysis of sugars in dry wines. Liquid-chromatography-mass spectroscopy (LC-MS) offered superior sensitivity and resolution compared to the relatively new technique of CE-MS for the analysis of wine phenolics. LC-MS was further applied for the efficient and sensitive analysis of non-coloured phenolics and anthocyanins in wine. Negative- and positive electrospray ionisation, respectively, were used in conjunction with an ion-trap mass analyzer, for the identification of 34 phenolics and 31 anthocyanins in red wine samples. Complementary CE and LC methods were developed to allow the identification of artificial dyes in red wines, added illegally to improve their colour. Also, the application of stir bar sorptive extraction (SBSE) with liquid desorption and micellar electrokinetic chromatography (MEKC) for the analysis of bitter acids in beer is reported. In the second part of the thesis, the analytical results obtained for South African red and white wines were evaluated. Following comparison of the results with literature reports, several pattern recognition techniques were employed. A classification function obtained by linear discriminant analysis (LDA) was used to classify both red and white wines according to variety, based on their chemical composition. This classification is achieved independent of the factors of wine age or geographical origin, making it useful for authenticity evaluation. / AFRIKAANSE OPSOMMING: Hierdie studie het as primêre doel die verbetering van bestaande analitiese metodes vir die analise van nie-vlugtige komponente in wyn. In die lig hiervan, is eerstens 'n toepaslike monster-voorbereidingstegniek, gebasseer op soliede fase ekstraksie (SPE), ontwikkel vir die gelyktydige analise van organise sure, suikers en fenoliese komponente vanuit die wyn matriks. Vervolgens is moderne analitiese rtietodes ondersoek en gepastde skeidingstegnieke is ontwikkel vir die verskillende chemiese wyn-komponente. Kappillêre elektroforese (CE) en hoë-druk vloeistof-chromatografie (HPLC) in kombinasie met verskeie deteksie-metodes is vergelyk. Hieruit is 'n verbeterde CE metode vir die analise van organise sure is ontwikkel, terwyl HPLC in kombinasie met refraksie-indeks- en verdampings lig verstrooiings deteksie (ELSD) die beste resultate lewer vir die analise van suikers in droë wyne. Die toepasbaarheid van vloeistof-chromatografie met massa spektrometriese deteksie (LC-MS) vir die analise van fenoliese komponente is gedemonstreer, terwyl CE-MS onvoldoende resolusie en sensitiwiteit toon vir die analises. LC-MS is vervolgens ook gebruik vir die identifikasie van 34 fenoliese verbindings en 31 antosianiede in rooi wyn. Komplementêre HPLC en CE metodes is ontwikkel vir die identifikasie van onwettige sintetiese kleurstowwe in' rooi wyn. 'n Addisionele monstervoorbereidingsstap, roerstaaf sorptiewe ekstraksie (SBSE), is saam met vloeistof-desorpsie en misellêre elektrokinetiese chromatografie (MEKC) gebruik vir die analise van hops bitter sure in bier. In die tweede deel van die tesis word die resultate verkry m.b.v. bg. tegnieke vir die analise van Suid-Afrikaanse rooi- en wit wyne, bespreek. Die resultate is vergelyk met waardes uit die literatuur, en verskeie statistiese metodes is gebruik om die data te ondersoek. Met behulp van chemometriese metodes is 'n klassifikasie funksie verkry wat die klassifikasie van Suid-Afrikaanse wyne volgens druifsoort, gebasseer op die chemiese samestelling van die wyne, toelaat. Die klassifikasie is moontlik, onafhanklik van die ouderdom of geografiese oorsprong van die wyne.

Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2016. / Wines contain a number of phenolic compounds, belonging to non-flavonoid and flavonoid complexes. Phenolic compounds in wine are responsible for wine colour, astringency, and bitterness. Saccharomyces cerevisiae yeast is normally used in winemaking but it has been proved to decrease the phenolic content in wines. Current research on the use of non-Saccharomyces yeast in winemaking has produced better quality wines than S. cerevisiae yeast therefore improving the sensory profile of wine. This study evaluated effect of Torulaspora delbrueckii yeast on the phenolic content of experimental wines derived from Chenin blanc grapes. A reversed phase high-performance liquid chromatographic (RP-HPLC) method was used for the identification and quantitation of the phenolic compounds. The difference test method was used to determine the sensory attributes of wines. The data was subjected to analysis of variance to compare treatment differences between the wines and principal component analysis to establish possible correlation between the data sets. Furthermore, a gas chromatographic-flame ionization detection method (GC-FID) was used for the quantification of volatile compounds in the wines. In this work, wines made with T. delbrueckii strain M2/1 had high concentration of (+)-catechin, caffeic acid, ferulic acid and p-coumaric acid in all studied vintages. Wines made with VIN13 had higher concentrations of flavan-3-ols, compared to wines made with M2/1 and 654. In sensory evaluation, M2/1 wines were prominent in astringency and complexity. Yeast strain M2/1, also attributed to body and complexity of the wine. However, in this study no correlations were observed between the phenolic content and sensory attributes and vice versa. The quality of wine cannot be concluded by chemical or sensory analysis alone, but the data sets are complementary. Although the phenolic concentration of wines made with S. cerevisiae strain (VIN 13) and T. delbrueckii (M2/1) were similar in measured phenolic concentrations, they had different sensory attributes. Wines made during the 2013 vintage indicated the importance of the use of a strain with higher enzyme activity and high fermentation rate. There is minimal to no skin contact in white winemaking. Therefore, the use of a yeast strain with an increased enzyme activity can facilitate the extraction of phenolics from grape, resulting in wine with improved quality.