The estuarine sheepshead minnow (Cyprinodon variegatus) is the most eurythermic fish species, with a thermal tolerance window between 0.6°C and 45.1°C. However, little is known about the physiological mechanisms that allow this species to survive this temperature range. In order to understand how sheepshead minnow physiology is affected by temperature acclimation and acute changes in temperature, I conducted research on this species using a multi-level approach. I began at the organismal level, and examined the effects of these temperature changes on the sheepshead minnow's metabolic rate and swimming performance. The next chapter investigated the effects of changing temperatures on cardiac function (i.e., tissue/organ specific effects). In the final chapter, I conducted research at the sub-cellular level, and determined how mitochondrial bioenergetics / function is impacted by changing temperatures. This research shows that while sheepshead minnows are able to sustain heart function and mitochondrial respiration over a broad range of temperatures; they also display a plastic temperature response which is associated with the downregulation of standard metabolic rate and cardiac remodeling to maintain force generation. Collectively, these physiological responses may contribute to the sheepshead minnow's ability to maintain physiological and organismal function across a large temperature range.

Understanding how organisms respond to climatic variability and novel conditions is becoming an increasingly important task for ecologists. For ectotherms in the northern hemisphere, the response to cold is of special interest, considering that poleward range expansion events and increasing variability of temperatures during winter are already being observed as consequences of a warming planet. Though direction of change in physiological variables in response to cold is well studied in ectotherms, the extent to which traits can change and the rate at which they can change is not. We compared the extent and rate of change in cold tolerance (CTmin) between two long-term captive populations of the Italian wall lizard (Podarcis siculus) during a lab cold-acclimation treatment. Heat tolerance (CTmax), thermal preference (Tpref), temperature dependent rates of oxygen consumption (SMRO2), and temperature dependent rates of water loss (EWL) were also compared between Italian wall lizards previously introduced to Long Island, NY and San Pedro, CA before and after the lab cold acclimation treatment. Because our study coincided with a cold snap during the spring 2018 season for the San Pedro, CA population, we also studied the effects of cold acclimatization on wild lizards from the CA population. After initial lab acclimation of the lizards to laboratory conditions, SMRO2 at 15°C and EWL at 10°C were higher in NY lizards compared to CA lizards. Lizards from the two populations did not differ in any other variables measured before the cold acclimation treatment. We found that lizards from the NY population experienced an 80% decrease in CTmin following a switch from 20°C:18°C to 17.5°C:16°C (12h light:12h dark) acclimation treatment. Lizards from the CA population did not decrease CTmin in response to the same cold acclimation treatment. Overall, NY lizards decreased CTmin, CTmax, and Tpref following cold acclimation, whereas CA lizards decreased CTmax only. Wild CA lizards decreased CTmax following the cold spring 2018 season in a manner similar to that of lab acclimated NY and CA lizards, suggesting that these lizards do not maintain a high CTmax when the environment is unlikely to expose them to high temperatures. Thermal sensitivity (Q10) of SMRO2 and EWL was lower in NY lizards, suggesting physiological adaptation to fluctuation in diurnal temperatures. The ratio of CO2 produced to O2 consumed (respiratory exchange ratio, RER) measured at 15°C increased in NY lizards following cold acclimation suggesting an increased use of carbohydrates and/or an increased production of lipids in the colder conditions. These responses in combination with the higher observed plasticity in NY lizards are in accordance with the climatic variability hypothesis, which predicts that organisms from more variable climates will be better adapted to physiologically respond to variable conditions. The higher capacity for physiological plasticity may explain the relatively high success of P. siculus in NY and other northern U.S. states. By describing the rate of change of CTmin during cold acclimation we hope to better understand how these lizards minimize the risk of low temperature exposure during winter. We ultimately hope to incorporate the rate at which cold tolerance can change into predictions of species distributions and hypothesis tests investigating the relationship between climatic variability and the rate at which animals can exhibit plasticity.

The purpose of this research is to expand the understanding of certain properties of electrodes used for electrical bioimpedance measurements. Specifically, this work investigates the acclimation effect of the skin-electrode interface contact impedance. It also attempts to study the relationship between electrode spacing and amplitude of bioimpedance pulsatile signals. It was found that as soon as dry electrodes are placed on the skin, the contact impedance exponentially decreases until it reaches a constant level. The acclimation time, time to reach a constant contact impedance, is dependent of the electrode size and frequency. Increasing the size of the electrode, as well as increasing the frequency, decreases the acclimation time. The acclimation of wet electrodes was also studied, and it was found that changes in contact impedance over time are negligible in comparison to the amount dry electrodes contact impedance change. However, the contact impedance of wet electrodes, instead of decreasing, tends to increase just slightly before reaching a steady state. Electrodes that do not carry current have contact impedance magnitudes similar to those that carried current after 60 minutes. This acclimation effect seems to be driven by the moisture level in the skin-electrode interface. As sweat and moisture build up with time when using dry electrodes, contact impedance decreases; and as the moisture in wet electrodes dries up with time, contact impedance increases. Capturing the small bioimpedance changes due to blood flow in the artery proved to be quite challenging under the circular orientation and with low levels of current injected. Only 5% of all the pulsatile data acquired had high enough quality to have a discernible pulsatile signal present on it. From the analysis of this 5% of data there were not conclusive results with regards the effect of electrode spacing on the pulsatile signal amplitude. However, the placement of the electrodes relative to the artery did seem to play a role on the pulse signal amplitude since the pulse amplitude seemed to peak when the center of the 4 electrodes was close to the artery. Pulsatile signal does not seem to be consistent throughout time; performing the same measurement 50 minutes apart sometimes resulted in the same or very similar measurements and other time the measurements were very different from each other. Despite the inconclusive results, the system for switching and selecting electrodes from an array of multiple electrodes along with the algorithm to determine the quality of the measured pulsatile signal proved to be efficient and serves as a foundation for developing a measurement system that can search and identify the the electrode configuration and placement that results in acquiring high quality signals.

Temperature-dependent weight gain of Gulf coast and northern strain walleye Sander vitreus fingerlings was determined by rearing fish in flow-through aquaria at different water temperatures (range 15-32°C). Maximum growth of Gulf coast and northern strain walleye occurred between 20 and 26°C, and weight gain of northern walleye exceeded that of the Gulf coast strain by 1.5 times. An acclimated chronic exposure method to assess upper thermal limits determined that walleye survived 35, 9, and 1 days at 33, 34, and 35°C, respectively. A post pond-rearing feed-training practice (21 d) at a mean density of 6,290 ± 1,247 fish/m3 using formulated feeds was successful (32-85% survival), and walleye continued to consume a formulated diet feed after stocked at densities of 12,250 and 24,700/ha into 0.04 ha earthen ponds. After 125 days, survival in ponds was poor (< 30%) and production varied substantially.

No description available.

No description available.

No description available.

No description available.

No description available.

<p>Under hypoxia mammals face many challenges, especially in terms of energy production. To conserve O2, mammals may enter a hypometabolic state or rely more heavily on anaerobic metabolism. However, the latter strategy is not a viable option during chronic hypoxia and other cellular changes are needed. Under chronic hypoxia, mammals have been predicted to alter their metabolic machinery in an attempt to increase the efficiency of ATP production to reduce the amount of O2 used by the mitochondria. One way efficiency is believed to increase is through a change in the composition of cytochrome c oxidase (COX). Cell culture experiments have shown a decrease in the COX4-1 isoform and an increase in the COX4-2 isoform under hypoxia, leading to an increase in the reaction efficiency of COX. In the present study, I observed an increase in the mRNA levels of COX4-2 after 24hrs of hypoxia. However, this change was not mirrored by corresponding changes at the protein level. Further, I examined the phosphorylation state of pyruvate dehydrogenase (PDH) as an indicator of PDH activity. Under chronic hypoxia resting mice exhibited a significant rise in PDH phosphorylation. This increase may represent a decrease in PDH activity and a decreased reliance on carbohydrate derived acetyl-CoA.</p> <p>I also explored the effects plastic changes in muscle during chronic hypoxia had on muscle metabolism during acute exercise. In hypoxic post-exercise mice, a significant increase in muscle lactate levels was observed compared to rest. This rise was not present in control mice, suggesting that acclimated mice were relying more heavily on anaerobic metabolism. However, there were no significant changes in PDH phosphorylation in post-exercise mice which could help to explain elevated muscle lactate levels.</p> / Master of Science (MSc)