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Effect of microencapsulated diets supplemented with a genetically modified bacteria on the growth and survival of Penaeus indicus postlarvaeSirvas Cornejo, Susana January 1999 (has links)
No description available.
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Study on the characteristics of Aeromonas hydrophila infecting on the skin of grouperWang, Hsiao-ting 14 January 2004 (has links)
In recent years, the skin and muscle hemorrhagic ulcer occurred in grouper hatchery, suggesting that the bacteria extracellular protease was involved. In this study I surveyed the extracellular protease activity of marine bacteria and investigated the characteristics of the pathogen isolated from diseased grouper. The sampling sites of background survey were located along the coast of Taitung. The cultured media were MB2216 and PPESII. The extracellular protease activity was measured using milk seawater medium (MS) and dye-modified proteins of azocasein and azoalbumin. The extracellular protease activities of the marine bacteria were 1~5 U/mg. The average protease activities of the bacteria from MB2216 media were higher than those from PPESII media. The extracellular protease activity assay using azocasein and azoalbumin had similar tendency but the result by the clear zone measurement on the MS medium was of high variations. The FI-02 isolated from the diseased grouper skin was identified as Aeromonas hydrophila by the biochemicial characteristics and 16S rDNA sequence analysis. FI-02 was able to grow on the LB broth with 0~4% NaCl. The FI-02 was resistant to ampillicin, cefazolin, erythomycin, sulfisoxazole, whereas it was sensitive to streptomycin, doxycline, norfloxacin, cefotazime, oxolinic acid, and tetracycline. When FI-02 growing in the four media, the extracellular protease activities were higher than 4 U/mg. The highest protease activity was 6.08 U/mg when the bacteria grew on the LB medium. After heating on 27, 37, 42 and 56¢J for 20 mins, the protease activities remained the same. In the investigation of the fish immunity, owing to the complex compositions of the fish serum, no conclusion can be drawn on the specificity of the antibody against the bacterium.
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Comparative pathogenesis of experimental Aeromonas hydrophila infections in mice and goldfishBrenden, Rita A. Huizinga, Harry W. January 1984 (has links)
Thesis (Ph. D.)--Illinois State University, 1984. / Title from title page screen, viewed May 20, 2005. Dissertation Committee: Harry Huizinga (chair), Marcia Miller, Tak Cheung, Mathew Nadakavukaren, Anthony Liberta. Includes bibliographical references (leaves 209-222) and abstract. Also available in print.
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An investigation of prevalence of Aeromonas hydrophila and of measures of immune function for frogs used in the Ontario baitshop industry : implications of past and present practices /McRuer, David L. January 1900 (has links)
Thesis (M. Sc.)--Carleton University, 2001. / Includes bibliographical references (p. 62-71). Also available in electronic format on the Internet.
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Exoprotease Production by Aeromonas hydrophila in a Chemically Defined MediumAnderson, Paulette S. (Paulette Sue), 1952- 05 1900 (has links)
Wretlind, Heden, and Wadstrom found ammonium sulfate to be inhibitory for the formation of extracellular protease in Aeromonas hydrophila grown in Brain Heart Infusion medium. They demonstrated by manipulating the iron and zinc content within their medium that it is possible to differentially affect the accumulation of hemolysin and protease by A. hydrophila grown in batch culture. Further manipulation of the composition of this medium was done in the present study to determine the effect of other components on the production of protease. The purpose of this study was to determine the factors affecting the level of A. hydrophila protease produced in a chemically defined medium.
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Development and Visualization of Bioluminescent Virulent Aeromonas hydrophila in Live CatfishOzdemir, Eda 10 August 2018 (has links)
Virulent Aeromonas hydrophila (vAh) is an important emerging bacterial pathogen causing motile Aeromonas septicemia (MAS) in farmed catfish. Understanding the pathogenicity of the disease is essential for the development of preventive measures. In this study, we aimed to develop a bioluminescent virulent A. hydrophila (BvAh) strain to understand the pathogen-host interactions during infection. To achieve this, a new bioluminescence expression plasmid, pAKgfplux3, was constructed and mobilized to vAh. Catfish were challenged with BvAh using immersion, injection, and adipose fin clip procedures, and bioluminescence signal was tracked in live catfish during infection. We developed a novel BvAh strain for the first time, conducted imaging of BvAh in live fish, detected infection routes and attachment sites of the pathogen, and determined target organs, which provided new insights on the pathogenesis of vAh. MAS progressed better in fish when protection of skin was bypassed. Abraded skin seems to provide a potential portal of entry during vAh infection.
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Molecular characterisation of virulence factors of Aeromonas hydrophila /Wong, Christopher Yew Fook. Unknown Date (has links)
Thesis (PhD)--University of South Australia, 1997
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An investigation of carbohydrate-reactive outer membrane proteins of mesophilic aeromonadsQuinn, Diana Marie January 1995 (has links)
Thesis (PhD (Health Science) in Medical Laboratory Science)--University of South Australia, 1995
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An investigation of carbohydrate-reactive outer membrane proteins of mesophilic aeromonadsQuinn, Diana Marie January 1995 (has links)
Thesis (PhD (Health Science) in Medical Laboratory Science)--University of South Australia, 1995
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Patógenos emergentes (aeromonas hydrophila, vibrio vulnificus e plesiomonas shigelloides) e resistência antimicrobiana em mexilhões na Baía de Guanabara: implicações para a saúde pública / Emergent Patógenos (aeromonas hydrophila, vibrio vulnificus and plesiomonas shigelloides) and resistance antimicrobian in mussels in the Baía de Guanabara: implications for the public healthPereira, Christiane Soares January 2003 (has links)
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Previous issue date: 2003 / O ecossistema aquático é habitado por mexilhões, animais filtradores que refletem a qualidade ambiental através da sua análise microbiológica. Neste estudo pretendeu-se avaliar a presença de patógenos, clássicos (Salmonella sp.) e emergentes (Vibrio vulnificus, Aeromonas hydrophila e Plesiomonas shigelloides), a partir de mexilhões in natura e pré-cozidos isolados de uma Estação Experimental de Cultivo de Mexilhões situada em Jurujuba, Niterói, Rio de Janeiro. Embora, não tenha sido identificado o patógeno Salmonella sp., as análises laboratoriais realizadas permitiram o isolamento de diversas espécies de microrganismos pertencentes às famílias Vibrionaceae e Aeromonadaceae, algumas com relevante potencial patogênico (Vibrio parahaemolyticus, V. vulnificus e A. hydrophila). Diversos microrganismos isolados apresentaram perfil de resistência a antimicrobianos utilizados na clínica humana (ampicilina, tetraciclina, nitrofurantoína, sulfametoxazol-trimetoprim e pefloxacina). Considerando a relevância epidemiológica dos agentes patogênicos identificados e os resultados obtidos nesta investigação, faz-se urgente alertar as autoridades sanitárias quanto à presença desses patógenos na cadeia alimentar e no ambiente. Dada sua capacidade de causar enfermidades diversas nas populações humanas após consumo de mexilhões in natura ou pré-cozidos, torna-se premente e de extrema importância o desenvolvimento de estratégias de vigilância epidemiológica e sanitária, assegurando, entre outras medidas, o monitoramento constante dos mexilhões oriundos da Baía de Guanabara, no sentido de prevenir ou minimizar os riscos nas diferentes etapas da sua extração e comercialização.
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