Made available in DSpace on 2014-08-20T13:44:28Z (GMT). No. of bitstreams: 1 dissertacao_loraine_rodrigues_jardim.pdf: 1137522 bytes, checksum: de7cff66f6911caeea51266527680966 (MD5) Previous issue date: 2012-08-13 / The occurrence of invasive plants can alter crop yields, because these plants often grow so strong, competing for light, water and nutrients, affecting seed production. The taxonomic identification of these species is the first step in planning a control program. The objectives of this work are: develop and validate a computer system to support the identification of units spread of invasive species; differentiate seeds of three species of quarantine pests Amaranthus genus of three species of the same genus harmful tolerated by analyzing the morphology inside and outside of their seeds. This work is organized into two chapters: Chapter 1 - describes the process of building a computer system to support the identification of units spread of invasive species. The database of the computer system was composed of morphological descriptions, drawings and photographs of the morphological structures of 198 seeds of invasive species. We developed a tool (available on the Web) to use the database built, providing morphological search by entering information on the morphology of seed and/or dispersion unit that seeks to identify the user. We conducted a test system functionality, which consisted of a questionnaire assessment while using the system. The system proved effective in identifying species, aiding the identification of invasive species. The creation of the database with the information and proposed structure meets the demand of the identification system. Chapter 2 - presents the morphological differentiation of the seeds of six species of the genus Amaranthus, seeking to differentiate species of harmful species quarantine pests in Brazil. We analyzed morphological descriptions of six species of the genus Amaranthus produced and a dichotomous key to highlight morphological able to differentiate species. The visualization of the structure of the embryo by slitting the seed, allows to differentiate the species of Amaranthus. Detailed analysis of the morphological internal and external seeds of Amaranthus is effective in the identification and differentiation of species. / A ocorrência de plantas invasoras pode alterar o rendimento das culturas, pois estas plantas frequentemente crescem de maneira robusta, competindo por luz, água e nutrientes, afetando a produção de sementes. A identificação taxonômica destas espécies é o primeiro passo no planejamento de um programa de controle. Os objetivos deste trabalho são: desenvolver e validar um sistema computacional de apoio à identificação de unidades de dispersão de espécies invasoras; diferenciar sementes de três espécies de pragas quarentenárias do gênero Amaranthus de três espécies nocivas toleradas do mesmo gênero, através da análise da morfologia interna e externa das suas sementes. Este trabalho está organizado em dois capítulos: Capítulo 1- descreve o processo de construção de um sistema computacional para apoio à identificação de unidades de dispersão de espécies invasoras. O banco de dados do sistema computacional foi composto por descrições morfológicas, desenhos das estruturas morfológicas e fotografias de 198 sementes de espécies invasoras. Foi desenvolvida uma ferramenta (disponibilizada na Web) para utilizar o banco de dados construído, oferecendo busca por características morfológicas, através da inserção de informações da morfologia da semente e/ou unidade de dispersão que o usuário procura identificar. Realizou-se um teste de funcionalidade do sistema, que consistiu na aplicação de questionário de avaliação durante a utilização do sistema. O sistema mostrou-se eficaz na identificação de espécies, auxiliando a identificação de espécies invasoras. A criação do banco de dados com as informações e a estrutura propostas atende a demanda do sistema de identificação. Capítulo 2- apresenta a diferenciação morfológica das sementes de seis espécies do gênero Amaranthus, em busca de diferenciar espécies nocivas de espécies pragas quarentenárias no Brasil. Foram analisadas descrições morfológicas de seis espécies do gênero Amaranthus e elaborada uma chave dicotômica para destacar características morfológicas capazes de diferenciar as espécies. A visualização da estrutura do embrião, mediante corte longitudinal da semente, permite diferenciar as espécies de Amaranthus. A análise detalhada das características morfológicas internas e externas das sementes de Amaranthus é eficaz na identificação e diferenciação das espécies.

Master of Science / Department of Agronomy / Johanna A. Dille / No-till producers can manage weeds by including cover crops during the fallow phase as part of an integrated weed management plan. Field experiments were conducted between 2007 and 2009 to quantify the influence of cover crops on weed emergence, biomass accumulation, and seed production. Field experiments were established near Garden City, KS with winter wheat or fallow as main plots and cover crop treatments as subplots including five spring- and five fall-sown individual or mixtures of crop species and a no-cover chemical fallow. Separate1-m2 quadrats were seeded with kochia or downy brome at 500 seed/m2. Kochia density was reduced by 75% and biomass reduced by 88% in fall-sown cover crops compared to chemical fallow across growing seasons. Spring-sown cover crop mixtures reduced kochia biomass in 2009 when kochia emergence was delayed. Downy brome biomass decreased exponentially as cover crop biomass increased. A second field experiment was established near Manhattan, KS with soybean, winter wheat, or grain sorghum phases of the rotation as main plots and six cover crop treatments as subplots sown after winter wheat harvest. Paired Palmer amaranth 1-m2 quadrats were seeded with 500 seed/m2 in each cover crop subplot. One quadrat was protected from any herbicide application made to the cover crop or to the grain sorghum. Combining burndown application with high biomass-producing cover crops reduced Palmer amaranth emergence and biomass. Influence of cover crop presence reduced early season Palmer amaranth emergence in the subsequent grain sorghum phase. Optimal seeding rate of forage soybean sown in winter wheat stubble and its impact on Palmer amaranth and downy brome emergence and growth were evaluated in field studies established near Manhattan and Hesston, KS in 2008 and 2009. Soybean was no-till drilled after wheat harvest at five rates ranging from 100,000 to 600,000 seeds/ha. A no-cover chemical fallow treatment was included. Separate 0.5-m2 quadrats were seeded with Palmer amaranth at 100 seed/0.5 m2 or with downy brome at 250 seed/0.5 m2. Three termination methods evaluated were killing frost, glyphosate application, or crop rolling. Palmer amaranth density was not affected by treatments but biomass decreased as soybean seeding rate and crop biomass increased. Downy brome emergence was less with rolled or sprayed termination methods in one site year as timing of termination was optimal. High biomass producing cover crops sown during the fallow phase of a crop rotation reduced weed emergence, density, and biomass accumulation. Cover crops can be part of an integrated weed management plan in Kansas.

This study determined the concentrations of lead and cadmium in edible parts of Colocasia esculenta, Amaranthus spp.and Ipomoea batata cultivated on farms in industrially polluted sections of Nyabugogo Marsh in Kigali, Rwanda. The concentrations in all three crops exceeded European Union (EU) standards for metal concentration in food crops. C. esculenta roots (Taro) contained the highest concentration of lead (1.02 mg kg-1) and cadmium (0.56 mg kg-1), approximately ten and six times over the EU limits, respectively. Even though I. batata (sweet potato) contained the lowest concentrations of lead (0.75 mg kg-1), this is almost eight times the upper limit. The highest bioaccumulation factors (the ratio of plant metal concentration to that of the soil in which it is found growing) for both metals were observed in amaranth plants. The concentrations of lead and cadmium in the farm soils were all acceptable based on EU standards (300mg kg-1 for lead and 3mg kg-1 respectively. The average daily consumption by an adult in the community living around the Marsh and where some of the produce is sold is 50g of amaranth, 120g of taro and 180g of sweet potato. Based on the metal concentration and these rates of consumption, the daily dietary intake of lead by an adult in the community from amaranth, taro and sweet potato is 1 x 10-4, 3 x 10-4 and 4 x 10-4 mg kg-1 respectively. The daily intake of cadmium is 4 x 10-4, 1.7 x 10-4 and 1.2 x 10-4 mg kg-1 for amaranth, taro and sweet potato respectively. These metal intakes are well within the recommendations set forth by the World Health Organisation. The community also has access to multiple sources of dietary and non dietary zinc such as beans, milk and rain water collected from zinc coated roofing sheets, which serves to ameliorate the effects of cadmium. It is however worth noting that survey data may have yielded overestimates of these zinc sources, due to the conditions under which the surveys were conducted i.e. in the hearing of neighbors due to the cramped nature of housing, which may have prompted respondents to inflate consumption quantities of expensive food items. The calculated maximum recommended quantities for daily intake of the crops are very large and are unlikely to be consumed by the population i.e. >2kg of amaranth, >2 kg of taro and 3 kg of sweet potato per day for an adult. Additionally, because this is a poor community, access to such quantities of food on a daily basis is not likely. The community is therefore not exposed to health risks from consuming metal contaminated crops, largely because of the small quantities consumed. The local population is therefore at no immediate risk to exceeding metal consumption limits by consuming vegetables grown in the Nyabugogo Marsh, but the threats will likely increase if the pollution of the Marsh is not addressed.

Amaranth (Amaranthus caudatus L.) is an important pseudocereal in the Andes. The seed has excellent nutritional value (high in protein, essential amino acids, and minerals) and ample capacity for growth in diverse, harsh Andean subsistence-production conditions such as water deficiency, salt stress, and soil mineral nutrient deficiency. The objective of this study was to characterize and quantify the genetic diversity among a series of 178 mostly Peruvian amaranth genotypes using 96 biallelic single-nucleotide polymorphism (SNP) markers. A total of 96 co-dominant, biallelic markers were developed using KASPar™ assays on a 96.96 Fluidigm EP1 array system. The 178 amaranth genotypes included white-opaque and white-translucent (vitreous) putative A. caudatus seed types, along with black-seeded A. hybridus and brown-seeded types, all isolated from among 48 accessions of the CICA-UNSAAC collection. Variation among and within samples and accessions was compared for empirically determined clusters (northern, north-central, south-central, and southern Peruvian Andes). Variation was highest within samples for all groups, but only in white-seeded amaranth was the p-value significant (17.43). The greatest variation among samples was found in the vitreous-seeded group (99.35). The highest average observed heterozygosity within-groups (Ho) was 0.19 in the brown-seeded group, and expected within-group heterozygosity (He) was highest in the vitreous-seeded group (0.359). Cluster analysis (UPGMA), PCA and PCO results partitioned the amaranth accessions into six discrete clusters. Clusters did not manifest obvious structure among accessions, which indicates that genetic diversity has been conserved across a broad region of the Peruvian Andes. The diversity characterization pointed to a center of origin and domestication of A. caudatus in the Ayacucho-Cusco region of southern Peru.

Ethnobotanical and dietary questionnaires were used to assess the utilization of plants to treat the signs and symptoms of anemia by women in La Chapelle, Haiti. The usual diet of respondents was found to be low to intermediate in iron bioavailability. The ethnobotanical questionnaire showed that most respondents (82%) used plant-based home remedies to treat anemia Amaranthus dubius, Citrus aurantium, Corchorus olitorius, Moringa oleifera, Phaseolus vulgaris and Portulaca oleracea used in the diet and as remedies for anemia were analyzed. Amaranthus dubius was found to have the higher in availability by in-vitro dialysis, 30%. The intra species variation in iron availability was influenced by storage and cooking times. The interspecies variation in iron availability was explained by the acidity of the plant species' cooked homogenate (r = 0.4168, p = 0.007).

Spiny amaranth is a problematic weed of heavily grazed pastures in Kentucky and surrounding states. The first objective was to evaluate spiny amaranth control when herbicides are applied before and after emergence. Spiny amaranth seed collected in 2008 were seeded in rows in the fall (November) and the following spring (March) in fields located near Lexington and Princeton, KY. Treatments consisted of five application dates and five herbicides plus an untreated control arranged in a split-split plot design. The following parameters were measured: fresh weight, plant height and percent visual control. At both locations pendimethalin applied in November, March and April before spiny amaranth emergence gave the greatest control and significantly reduced fresh weight biomass compared to other treatments. June applications of 2,4-D reduced plant height and provided 80 control. Fresh weight biomass and height were also reduced with dicamba, aminopyralid and aminocyclopyrachlor applied in June compared to pendimethalin and the untreated control. A soybean bioassay was conducted to measure soil dissipation of aminopyralid, a common pasture herbicide active ingredient. Soil samples were collected from two sites in Lexington and Princeton. During a season of above average rainfall aminopyralid had dissipated from the soil within 16 weeks at Lexington and by 4 weeks at Princeton.

<p>Tall waterhemp management in agronomic crops continues to be an increasing problem due to widespread resistance to herbicides, including protoporphyrinogen oxidase (PPO)-inhibitors. With limited effective postemergence herbicides, especially in soybeans, research to further understand the selection of PPO-resistant (PPO-R) tall waterhemp and identification of new herbicide resistance mechanisms is crucial for improving weed management decisions in order to slow selection for herbicide resistance and prolong the effectiveness of PPO-inhibiting herbicides.</p> <p> Previous research has shown that soil-applied applications of PPO-inhibiting herbicides can increase the frequency of the PPO resistance trait (∆G210) in surviving tall waterhemp plants, even when applied in combination at the same ratio with the very long chain fatty acid inhibitor (VLCFA), <i>s-</i>metolachlor. Field experiments were conducted to determine if selection for tall waterhemp resistant individuals to PPO-inhibitors could be reduced when the soil residual activity of <i>s</i>-metolachlor persisted longer than the PPO-inhibitor herbicide. The frequency of ∆G210 in surviving individual plants increased as the fomesafen rate increased, but was independent of the rate of <i>s</i>-metolachlor. Additionally, heterozygosity of ∆G210 in surviving individuals did not change with any rate or combination of fomesafen and <i>s</i>-metolachlor. However, saflufenacil, standard PPO-inhibitor with relatively short soil residual activity, applied alone increased the number of homozygous PPO-R tall waterhemp by 15% compared to the high rate of <i>s</i>-metolachlor and the combination of saflufenacil and <i>s</i>-metolachlor. Furthermore, this research demonstrated that end of season control of tall waterhemp plays a more vital role in delaying a large-scale shift towards herbicide resistance through reduced seed production. This can be achieved through the combination of multiple effective herbicide sites of action, including soil residual PPO-inhibitors. Tall waterhemp control and density were greatest with the high rates of fomesafen plus <i>s</i>-metolachlor, which resulted in the lowest number of PPO-R tall waterhemp that survived herbicide treatment at the end of season.</p> <p> Prior to the research conducted in this thesis, the only known resistance mechanism to PPO-inhibiting herbicides in tall waterhemp has been the ∆G210 target site mutation. A previously developed TaqMan assay used to determine the presence or absence of the ∆G210 mutation has allowed accurate, high throughput screening of this mutation. However, suspected PPO-R tall waterhemp do not always receive positive confirmation indicating the presence of an alternative resistance mechanism. Identification of additional resistance mechanisms can provide valuable insight in regards to resistance to PPO-inhibiting herbicides as well as cross resistance to other herbicide modes of action, which can lead to improved tall waterhemp management decisions. Of 148 tall waterhemp populations collected across the Midwestern U.S., 84% of the populations sampled contained at least one PPO-R biotype with the ∆G210 mutation, although several individual plants across the Midwest U.S. exhibited phenotypic resistance to fomesafen that could not be explained by ∆G210. The percentage of PPO-R tall waterhemp without ∆G210 was 19, 5, 2, 1, and 2% for Iowa, Illinois, Indiana, Minnesota, and Missouri, respectively. Following the initial greenhouse screening, subsequent tall waterhemp populations were selected that exhibited low-, mid-, and high-level resistance to fomesafen that resulted in resistance ratios from 0.6 to 17X in response to fomesafen. This research documents the variability in fomesafen response to multiple tall waterhemp populations in addition to revealing the presence of additional resistance mechanism(s), other than the previously known ∆G210 mutation that has been the benchmark for resistance to PPO-inhibiting herbicides in tall waterhemp.</p> <p> Lastly, greenhouse and lab experiments were conducted to investigate the role of antioxidant enzymes with PPO-R tall waterhemp via ∆G210. The objectives of this research were to determine if the variability in resistance ratios for PPO-R tall waterhemp documented in greenhouse and field scenarios could be due to an enhanced antioxidant enzyme pathway. Basal levels of antioxidant enzymes in PPO-S populations were not different from PPO-R populations when pooled together by respective phenotype. However, enzyme activity of tall waterhemp populations varied at the individual level, but independent of the ∆G210 mutation. This indicates that an inherent enhanced antioxidant enzyme pathway does not cause the variability in fomesafen response in tall waterhemp. With the exception of glutathione reductase, antioxidant enzyme activity following fomesafen application was generally the same for PPO-R and PPO-S populations by increasing, decreasing, or remaining unchanged. Glutathione reductase activity in PPO-S populations decreased compared to PPO-R populations from 9 to 36 HAT. By 36 HAT, all antioxidant enzyme activity for PPO-S populations was lower compared to PPO-R populations most likely a consequence of more lipid peroxidation. This research shows that antioxidant enzyme activity correlated with fomesafen application and documents the variability observed within tall waterhemp populations with and without the ∆G210 mutation. </p>

No description available.

The thesis deals with the content of selected phenolic compounds in some species of the genus Amaranthus, in black elderberry (Sambucus nigra L.) and buckwheat (Fagopyrum esculentum M.). Phenolic compounds are a group of natural compounds exclusively vegetable character. Flavonoids represent only one group of phenolic compounds. Flavonoids show many positive biological effects, in particular act as antioxidants. Natural flavonoids may cause to prevent from coronary- heard diseases and other diseases associated with older age. In recent years the increased attention is paid to flavonoid investigation due to its biological effects. For the determination of phenolic substances there were used two independent analytical methods. There are the high-performance liquid chromatography (HPLC) and micellar electrokinetic capillary chromatography (MECC). The MECC method was used for determination rutin and free quercetin. The highest content of rutin was found in leaves of buckwheat (76,400 mg/kg of dry weight) and the lowest content of rutin was determined in buckwheat hulls. The highest content of rutin was observed in teas from buckwheat leaves and inflorescence. This amount of rutin corresponds with rutin content in more than two pills of Ascorutin (the most favourite flavonoid medicament in the Czech Republic) The HPLC method was used for quantitative determination of phenolic acids. The content of free quercetin was monitored in all samples. No free quercetin was found both in plant material and in samples of teas. The ethanolic extract from the elderberry inflorescence didn´t contain any free quercetin. Free quercetin wasn?t found in any further samples of teas, which were prepared by described methods.

M. Tech. (Biotechnology, Department of Health Sciences), Vaal University of Technology / Amaranthus tricolor (A. tricolor) is a nutritious vegetable crop that is used as a subsistence and cash crop in the rural areas in Africa. Its yield and production is severely limited by abiotic stresses such as drought. Mutation technology, using gamma irradiation, was previously employed as a tool to create genetic variation in order to select for lines with improved drought-tolerance. During irradiation, 160 Gy (Gray) was selected as the optimal dosimetry that allowed subsequent seed germination. The resulting mutant lines were screened over several generations under field and greenhouse conditions and seven promising drought-tolerant lines were selected. Here we report on physiological and morphological studies of two of these Amaranthus mutant lines (#2 and #5) to confirm the enganced drought-tolerance. Plants were grown in the greenhouse in plastic pots containing germination mix with fertiliser. They were exposed to 21 days of well-watered condition, 19 days of drought-stress conditions and 7 days of re-watering. shoot height, leaf area, protein content and relative water content (RWC) of the fresh and dry material were determined colorimetrically under well-watered and drought-stress conditions, while anthocyanin was only measured during well-watered conditions. Shoot height, leaf area, number of leaves per plant and the protein content were significantly reduced under water-stress conditions. Under well-watered condition mutant #5 grew faster with the shoot length significantly higher than mutant #2 and the wild type. Even though drought adversely affected shoot lenght, mutant#5 still performed better than mutant #2 and the wild type under drought-stress conditions. While under both well-watered and drought-stress conditions, the wild type plants had bigger leaf area compared to the two mutant lines. After 16 days of drought-stress conditions, all the leaves of the wild type plants were dried out, as a result no wild type plants recovered after 8 days re-watering. Meanwhile, both mutant #2 and #5 plants recovered significantly after 8 days of re-watering. The wild type was tolerant compared to the two mutant lines. Protein content for mutant #2 plants was higher under both well-watered and drought-stress conditions but was not significantly different from mutant #5 plants compared to the wild type plants after 19 days of drought-stress conditions. Furthermore, genetic diversity was examined in all the Amaranthus lines using random amplified polymorphic DNA (RAPD) analysis. Nineteen arbitrary RAPD markers were used of which two detected polymorphisms (OPA) 07 and OPA 16).