付記する学位プログラム名: 霊長類学・ワイルドライフサイエンス・リーディング大学院 / 京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第22878号 / 理博第4644号 / 新制||理||1668(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)准教授 足立 幾磨, 准教授 Michael Alan Huffman, 教授 高田 昌彦 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

Reef-building corals are cornerstones of life in the oceans. Understanding their interactions with microorganisms and their surrounding physicochemical conditions is important to comprehend reef functioning and ultimately protect coral reef ecosystems. Corals associate with a complex and specific array of microorganisms that supposedly affect their physiology and therefore can significantly determine the condition of a coral ecosystem. As environmental conditions may shape bacterial diversity and ecology in the coral symbiosis, ecosystem changes might have unfavorable consequences for the holobiont, to date poorly understood. Here, we were studying microbial community changes in A. hemprichii as a consequence of simulated eutrophication and overfishing over a period of 16 weeks by using in situ caging and slow release fertilizer treatments in an undisturbed Red Sea reef (22.18ºN, 38.57ºW). We used 16S rDNA amplicon sequencing to evaluate the individual and combined effects of overnutrification and fishing pressure, two of the most common local threats to coral reefs. With our data we hope to better understand bacterial population dynamics under anthropogenic influences and its role in coral resilience. Projecting further, this data will be useful to better predict the consequences of human activity on reef ecosystems.

Amphibians are osmotically-challenged due to their highly permeable skin and egg membranes. As such, biologists have mostly ignored their occurrence in saline habitats. The goal of this dissertation was to investigate the ability of amphibians to adapt to inhabit these osmotically-stressful environments. As freshwater resources are increasingly salinized due to a combination of anthropogenic and natural stressors, including road-deicing salt application, coastal storm events, and sea-level rise, it is important to understand the abilities and constraints of sensitive organisms, like amphibians, to persist in these environments. I began by exploring what is known about amphibians inhabiting saline habitats around the world, and reviewed information dating from the 1800s to the present, documenting over 100 species inhabiting inland and coastal environments. I then proceeded to investigate the evolutionary potential for one species in particular, the rough-skinned newt (Taricha granulosa) to adapt to increased salinity in its habitat. I documented the negative effects of salinity (both NaCl and MgCl2 – the two most commonly-used road deicers) on embryonic and larval survival and development through a series of experiments, and explored the importance of both developmental and evolutionary history with a stressor on survival. I investigated the evolutionary potential of salt-naïve populations through examining intrapopulation and interfamily variation in critical early life-history traits, and determined that there is high interfamily variation in the salt tolerances of offspring of individual females within a population, providing the raw material for natural selection and local adaptation. Finally, I discovered a population of newts living in the tidal area of a coastal stream, with a natural source of salinity. Through conducting a series of laboratory salt challenges and assays on osmotic, stress, and immune physiology, I was able to determine that newts in the tidal area appear to be physiologically adapted to increased salinity compared to newts in a freshwater area upstream. All this information suggests that amphibians, while still osmotically-challenged, may also not be helpless in the face of salinization, and populations may be able to locally adapt to habitats impacted by natural and anthropogenic sources of salinity.

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Anthropogenic global change is altering food web dynamics. Global change comprises factors, like temperature, sound, light and more. In this dissertation, I evaluate how two factors, sound and temperature, alter prairie communities. In Chapter 1, I test if sound influences grasshopper respiration rate, thereby altering diet. Some sound frequencies increased grasshopper respiration rate while others decreased respiration rate. Frequencies that elevated respiration rate led to grasshoppers consuming more carbohydrate-rich foods compared to protein-rich foods. This diet change stems from a carbon deficit due to increased respiration rate and could act as a mechanism by which sound pollution indirectly alters plant communities. In Chapter 2, I test the hypothesis that sound can alter grasshopper movement. In response to sound-induced stress grasshoppers may alter their movement in one of two ways: in situ and displacement. I found no evidence that grasshoppers, nor non-Orthopteran insects alter their movement in the presence of sound. This chapter provides foundational methods to evaluate sound for applications in conservation and management. Further research will improve techniques for grassland or agricultural systems. Temperature is another driver of community change. What is less understood is how warming influences predator-pollinator relationships. In Chapter 3, I ask if warming alters a spider that consumes pollinators in a prairie system. My results indicate that pollinators benefit when spiders are not on the flower. Warming shifts spiders down the plant, thus positively impacting pollinator-plant interactions. In addition, warming may benefit plants two-fold if spiders shift their diet to herbivores. This requires additional research, but it is evident that warming generates a positive indirect effect on plants. These chapters contribute to a growing understanding of how global change is restructuring ecosystems. While global change may alter population dynamics or lead to evolutionary change over longer time scales, behavioral responses happen rapidly and can drive ecological dynamics in the short term. My dissertation demonstrates that sound and temperature alter animal behavior that cascades to lower trophic levels. Thus, in addition to demonstrating the indirect effects of global change, these experiments contribute to growing literature on the importance of top-down control in shaping ecosystems.

Throughout the world, birds represent the primary type of wildlife that people experience on a daily basis. However, a growing body of evidence suggests that alterations to the acoustic environment can negatively affect birds as well as humans in a variety of ways, and altered acoustics from noise pollution has the potential to influence human interactions with wild birds. In this thesis, I investigated how anthropogenic noise impacts daily behavior as well as community structure of wild birds. In the first component of this thesis, I assessed the distance at which a bird initiates flight or escape behavior (i.e., flight initiation distance or FID) in varying acoustic conditions. I surveyed 12 songbird species from three foraging guilds, ground foragers, canopy gleaners, and hawking flycatchers, and I predicted FIDs to decrease, remain the same, and increase with noise exposure, respectively. Contrary to expectations, the canopy gleaning and flycatching guilds exhibited mixed responses, with some species exhibiting unchanged FIDs with noise while others exhibited increased FIDs with noise. However, FIDs of all ground foraging species and one canopy gleaner decreased with noise levels. In the second component, I examined the feeding of wild birds, an increasingly popular recreational activity throughout North America that promotes increased sense of wellbeing by connecting people with wildlife and nature. I tested how experimental noise influences abundance, species richness, community structure and foraging behavior of songbirds at maintained bird feeders. By measuring activity levels of all species that utilized the feeders exposed to intervals of quiet and noisy conditions, I found noise to be a significant predictor of community turnover. Specifically, noise exposure resulted in increased feeder activity for two species, and decreased activity for one species. I also confirmed previous research conducted in the laboratory indicating white-crowned sparrows decrease their foraging rate under noise conditions, presumably as a trade off with visual vigilance. Considering the interactions of humans and wild birds, the results from my two thesis components indicate that the acoustic environment can play a role in how species of different foraging guilds respond to birdwatchers and what species visit bird feeders.

Anthropogenic noise is an increasingly prevalent global disturbance. Animals that rely on the acoustical environment, such as songbirds, are especially vulnerable to these sounds. Traffic noise, in particular, overlaps with the frequency range of songbirds, creating masking effects. We investigated the effects of chronic traffic noise on provisioning behaviors and breeding success of nesting western bluebirds (Sialia mexicana) and ash-throated flycatchers (Myiarchus cinerascens). Because anthropogenic noise exposure has the potential to interrupt parent-offspring communication and alter vigilance behaviors, we predicted that traffic noise would lead to changes in provisioning behaviors, such as fewer visits to the nest box, for each species. We also predicted the noise to negatively influence one or more metrics reflective of reproductive success, such as nest success, clutch size, number of nestlings or number of fledglings. Importantly, we were able to eliminate self-sorting among individuals with respect to noise and other possible effects of traffic, such as collisions and pollution, by experimentally introducing traffic noise into nest boxes after clutch initiation using playback systems. Our results indicate no effect of traffic noise on S. mexicana reproductive measures, despite seeing changes in provisioning behaviors suggestive of an increased stress response. However, M. cinerascens experienced fitness consequences from chronic anthropogenic noise exposure when combined with the effect of year. Despite equal clutch sizes to flycatchers in boxes with no noise exposure, noise-treated flycatcher nests experienced a reduction in hatching success and subsequent metrics, such as number of nestlings or fledglings relative to control boxes, during the first study year. The year in which we witnessed the decline in nest success for ash-throated treatment individuals coincided with a significant drought year, suggesting that combinations of multiple stressors lead to nest abandonment. In recording provisioning behaviors during the fledgling stage, no difference in provisioning was found between treatment and control ash-throated flycatcher nests. We contrast these species-specific responses to recent observational work on both species in New Mexico and other studies that have examined fitness consequences of noise. Lastly, we stress the importance of these findings in addressing current conservation practices of nest box placement with respect to roads.

ABSTRACT The greatest driver of the current global biodiversity crisis is habitat loss. Roads are a major contributor to habitat loss because they destroy and fragment habitat, in addition to causing direct mortality. Animals may respond to roads either by avoiding them, thus leading to population isolation, or by attempting to cross them, thus potentially leading to increased mortality and, if so, also to population isolation. I studied the impact of road density on abundance of two northern snake species: the redbelly snake (Storeria occipitomaculata) and the garter snake (Thamnophis sirtalis). I hypothesized that roads are detrimental to snake populations due to road avoidance and road mortality. Therefore, I predicted that snakes should be less abundant in sites with higher road density in their surroundings. I deployed cover boards at 28 old field sites along a gradient of road density in 2020 and in 2021. I visited sites weekly and counted the number of individuals of both species. I captured fewer garter snakes at sites surrounded by more roads, and fewer redbelly snakes at sites enclosed by more roads. The effect of roads on number of snakes is modest, but could be indicative of decreasing population size, which could in turn lead to loss of ecological function. RÉSUMÉ Le plus grand moteur de la crise mondiale actuelle de la biodiversité est la perte d'habitat. Les routes contribuent grandement à la perte d'habitat parce qu'elles détruisent et fragmentent l'habitat, en plus de causer de la mortalité directe. Les animaux peuvent réagir aux routes soit en les évitant, entraînant ainsi l'isolement des populations, soit en tentant de les traverser, entraînant ainsi potentiellement une mortalité accrue et également l'isolement des populations. J'ai étudié l'impact de la densité des routes sur l'abondance de deux espèces de couleuvres nordiques : la couleuvre à ventre rouge (Storeria occipitomaculata) et la couleuvre rayée (Thamnophis sirtalis). J'ai émis l'hypothèse que les routes sont néfastes pour les populations de serpents en raison de l'évitement des routes et de la mortalité routière. Par conséquent, j'ai prédit que les couleuvres devraient être moins abondantes dans les sites avec une densité routière plus élevée dans leurs environs. J'ai déployé des plaques abris sur 28 sites de champs en friche le long d'un gradient de densité de routes en 2020 et en 2021. J'ai visité les sites chaque semaine et compté le nombre d'individus des deux espèces. J'ai capturé moins de couleuvres rayées et moins de couleuvres à ventre rouge aux sites entourés de plus de routes. L'effet des routes sur le nombre de couleuvres est modeste, mais pourrait indiquer une diminution de la taille de la population, ce qui pourrait à son tour entraîner une perte de fonction écologique.

Anthropogenic sound in Florida's waters and coastal waterways is most commonly caused by overwater development, marine traffic, and military activity. Overwater construction has increased over the years as a result of aging infrastructure and rising expansions around the United States, including more than forty US Naval facilities containing tens of thousands of feet of pier. Construction methodology, such as pile driving, has risen in shallow waters to build structures such as bridges, piers, and wind farms, with significant consequences for marine life and the environment. More precisely, pile driving activities generate significant decibel levels in the surrounding marine environment. Measurements taken from hydrophones placed in the water near the construction site indicate that the high sound pressure levels produced may be harmful to marine life and the environment. As a result, standards have been established to help alleviate and decrease the possible harm that high decibel sound levels may produce. However, these additional steps increase the overall cost of the construction project. This thesis focuses on replicating the pile driving process using finite element modeling to hydroacoustic parametric study of pile driving-induced anthropogenic sound in neighboring Florida seas, as well as the possible environmental impact of the state's numerous naval base piers. The modeling predictions can then be used to identify the distance from the pile at which marine life and the environment are no longer adversely affected. In addition, computer modeling can reduce construction costs when compared to on-site sensors and monitoring. / Master of Science / Over recent years there has been an increase in the amount of manmade noise in Florida and its coastal waterways due to overwater construction, marine traffic, and military activities. Pile driving construction has increased in shallow waters to build infrastructure, which includes bridges, piers, and wind farms, resulting in a negative impact for marine life and the environment. Federal agencies have established guidelines to ease the harmful effects construction has on marine life and the environment. However, there is concern that these recent guidelines may not properly consider all the geometric and hydrographic variables of manmade noise that affect the high sound exposure levels during pile driving. With a more accurate understanding of the sound generation produced from pile driving, predictions can assist with sound mitigation to ensure less harm to the marine life and environment. In turn, construction companies and government agencies informed with this enhanced understanding can make better decisions that lead to fewer (or possibly eliminate) transmission loss discrepancies and costly noise mitigation measures. Consideration of the marine environment is one of the United States Navy's top priorities with naval stations located throughout the State of Florida that possess thousands of feet of waterfront structures, including piers, requiring routine maintenance and construction. This thesis models the pile driving process through finite element modeling in COMSOL Multiphysics computer software, testing the various parameters that Florida waters may encounter with pile driving on the surrounding coast as well as naval bases.

The Mekong River Basin (MRB) is famous for its rice farming and export and produces more than 20 million tons of rice per year. Rice production depends on climate, irrigation, soil fertility. However, this region is adversely impacted by several environmental concerns like nutrient deficiency from sediment and saltwater intrusion. The decrease in sediment deposition in the Mekong basin is caused by a number of factors. In China, Lao PDR, and Vietnam, the hydropower generation from dams has improved people's overall living standards, leading in more dams being built or planned in the future. However, dam construction work is adversely impacting the overall salinity condition in this region by reducing upstream flow. Upstream lower flows during the dry season contributes to the increased salinity in the lower Mekong Delta. In addition to these, multiple dams in the upper and middle region of the Mekong basin are trapping sediments and decreasing it in the lower zones. This study found that the reservoirs, built by China between 2008-2015, has reduced the sediment load at all five stations considered in the study. When a reservoir is removed from the model, the sediment load is increased which showed the substantial impact of reservoir construction on sediment load in this area. The landuse pattern is another factor for variability of the sediment yield in the study area. Forest area contributes to higher sediment production whereas agricultural area results in lower sediment yield. The GFDL RCP (4.5) and GFDL RCP (8.5) future climate change projection scenarios used in this study also demonstrated substantial variability in the precipitation pattern for the study region. GFDL RCP (4.5) scenario resulted in a lower sediment yield during the dry season. On contrary to that, GFDL RCP (8.5) showed higher sediment yield due to higher precipitation during the wet season. The severe salinity impact was observed in the Cai Nuoc, Nam Can, and Thanh Phu districts. In Ca Mau province, the observed salinity is highest among the provinces of the study area during dry season (February to May), about 12-14 PPT (parts per thousand) whereas the lowest level of salinity (less than 1 PPT) was observed in the Dong Thap and Vinh Long provinces. This salinity intrusion is adversely impacting the rice production in the study area. In the year 2000, rice production in the Ca Mau province was about 100-150 thousand tons. But salinity intrusion is drastically reducing the rice production in this area, about 10-30thousand tons per year during 2015-2017. Rice production is increasing in the upper deltaic part of the Mekong Delta region where preventive measures were taken. / Master of Science / The Mekong River Basin (MRB) is famous for its rice farming and export and produces more than 20 million tons of rice per year. The rice production is governed by rainfall, temperature, irrigation, soil fertility etc. However, this region is adversely impacted by multiple environmental concerns like nutrient deficiency, sediment concentration, and salinity. The decrease in sediment deposition in the Mekong basin is caused by several factors. In China, Lao PDR, and Vietnam, the hydropower sector has improved people's overall living standards. As a result, more reservoirs are being constructed or planned to be constructed in the future. But this dam construction work is adversely impacting the overall salinity condition in this region. Upstream flows rate during the dry season (February to May) contributes to the increased salinity condition in lower Mekong Delta. In addition to these, multiple dams in the upper portion of the Mekong basin are trapping sediments and decreasing it in the lower region. From the analysis performed in this study it was found that the reservoirs, built by China between 2008-2015, has reduced the sediment load at all five stations. When the reservoir is removed from the model, the sediment load is increased implying the substantial impact of reservoir construction on sediment load in this area. The landuse pattern is another dominating factor for variability of the sediment yield in the study area. Forest area contributes to higher sediment production whereas agricultural area results in lower sediment yield. Two future climate projection scenarios considered for this study are the GFDL RCP (4.5) and GFDL RCP (8.5). These two scenarios also demonstrated substantial variability in the precipitation pattern for the study region. The severe salinity impact was observed in the Cai Nuoc, Nam Can, and Thanh Phu districts. In Ca Mau province, the observed salinity is highest among the provinces of the study area during dry season (February to May), about 12-14 PPT ((parts per thousand) whereas the lowest level of salinity (less than 1 PPT) was observed in the Dong Thap and Vinh Long provinces. This salinity intrusion is adversely impacting the rice yield in the study area. In the year 2000, rice production in the Ca Mau province was about 100-150 thousand tons. But salinity intrusion is drastically reducing the rice production in this area, about 10-30thousand tons per year during 2015-2017. Rice production is increasing in the upper deltaic part of the Mekong Delta region where preventive measures were taken.