After joining in the World Trade Organization (WTO), the Taiwan¡¦s steel import tariff has been comprehensively reduced to almost nil customs duties (duty-free) since the year 2004. As a result of overwhelming importation of steel products and continuous investment in enhancing and enlarging output capabilities made by local steel manufacturers, the domestic steel markets are showing a tendency to a surplus status of supply over demand. However, due to the increasingly strong demand of steel materials made by the China mainland in the recent years, it has been not only greatly helped put off the execution of the world-wide scattered anti-dumping trade remedy assistance measures since the year 2000, but also benefited the domestic major steel manufacturers adjusting and improving their market structure and profit advantages in time. This enables the local steel producers to gradually enlarge their export quantity and aggressively expand their steel output on China mainland in the meantime. While the steel industries are boomingly developing, the international steel prices are rising and the steel market bargains are boosting, I am firmly confident that only the steel enterprises are able to concentrate their attention on what the anti-dumping measures formulated by WTO are stated, and to take appropriate dealing actions in the meantime, the competitive edge will be able to be elevated by the domestic steel producers. Therefore, to immediately setup information system on the forecast of dumping prediction and the knowledge of anti-dumping measures is a prerequisite to facilitate enhancing the enterprise¡¦s competitive edge and superiority. The results of this research are made into two fields on the application of a dumping prediction about the coated/galvanized plate steel products. i.e. (1) In line with the WTO regulations and the US anti-dumping law, I have made various collection concerning the information of law articles, dumping items, documents as well as miscellaneous information and have combined them into one consolidated concept to construct an up-dated dumping pre-warning alarm system. (2) Further study was made on the pre-warning subject concerning price negotiations and price finalizations of the S company¡¦s marketing department between the phases of order reception and goods issue. The result I discovered is that it is critically important for the enterprise to systematically pin down and appropriately structure its sales prices in advance and timely notice the pre-warning alarm measures in linking with the marketing model team before the entrepreneur is facing an international anti-dumping complaint or accusation in this regard.

The spin polarized first principles pseudofunction (PSF) method with the local spin density approximation (LSDA) has been used to calculate total energies, electronic structures and total and partial densities of states for bulk MnTe in the NiAs structure for both anti-ferromagnetic and ferromagnetic states under the pressure. For the pressure range considered in this study, the anti-ferromagnetic state is thermodynamically more stable than the ferromagnetic state in agreement with experimental observation. For the anti-ferromagnetic state, MnTe has an indirect band gap, which decreases linearly with the increase of the pressure in agreement with experiment. The calculated valence band and conduction band are found to broaden with the increase of the pressure, which can be attributed to the pressure induced shortening of the Mn-Te bond length that enhances hybridization between Mn and Te orbitals.

This research studies the degree of publicity made by Kaoshiung City Government and the anti-corruption condition caused by the degree of administrative transparency. This study also provides further understanding about present Kaoshiung City Government immediate online services, including people¡¦s requests and claims to Mayor¡¦s inbox and the anti-corruption advocated by the Department of Civil Service and Ethics: To explore how fast and how responses are made to citizens through Internet, and also to become the basis of anti-corruption index. To understand the expectations of civilians towards the overall government¡¦s publicity system. To establish a anti-corruption documentation system to provide sources of subsequent denotation of policy and knowledge transformation To design and suggest Kaoshiung¡¦s future anti-corruption policy, improve present file management and increase degree of publicity in order to achieve the goal of high quality and high efficiency To interview officials or others who involve with the administrative procedure, and provide concrete suggestions based on the content from the interview

In the development of the global software industry, what kind of development trend can we observe? Which kind of role does Microsoft act in the development of the global software industry? On the other hand, how to develop the software industry in China? What is the development procedure of the Chinese software industry? This paper concentrates on studying the relationship between Microsoft and China. After analyzing the entry strategy and management tactics of Microsoft, we can understand the delicate relation between Microsoft and Chinese government. And we may realize, in addition, why China does need Microsoft. How does China receive more advanced technologies through the competition between the software manufacturers? Because Microsoft has offered more investment and assistance, China will have the capabilities to develop its own software industry. In order to get more long-term development in China, Microsoft also needs to develop the good relations with Chinese government, from central government to the local governments, and utilizes these relations; Microsoft may obtain more favorable development chance in China.

Seafood including crustaceans, on ingestion, are known to provoke gastrointestinal as well as systemic allergic reactions. Crustaceans are aquatic arthropods with a chitinous exoskeleton and include shrimp, lobster, prawn and crab. Earlier studies in our laboratory have led to the identification and characterization of three allergens from shrimp, designated as Sa-I, Sa-I1 and Sa-III. The former two were shown to be heat stable proteins with a mol. wt. of 8.4 and 34 kDa respectively, while Sa-III was identified as tRNA Arg and TRNATyr ). Sa-II was found to be the major allergen contributing to more than 50% of the allergenic activity. There are several reports on the existence of cross-reactivity among atopic allergens, in particular food allergens. It is well known that individuals with shrimp allergy often complain of adverse reactions following the ingestion of other re1ated crustaceans. Recognition of crustacea as a group causing adverse reactions in sensitive individuals has a basis in the close phylogenetic relationship of shrimp, lobster, crab and prawn. Thus, one could expect appreciable similarity in the IgE binding epitopes of the offending allergens from related crustaceans. The present study was, therefore, aimed towards the identification of the major cross-reacting crustacean allergen and localization of its IgE binding epitopes. Cross-reactivity among a1lergens from shrimp, prawn, crab and lobster was evaluated by immunochemical methods. Antigenic cross-reactivity was established by immunodiffusion using shrimp-specific rabbit IgG. Competitive ELlSA inhibition experiments using sera of shrimp sensitive patients revealed a high degree of allergenic cross-reactivity between different crustaceans. SDSPAGE and immunoblot analysis using the sera of shrimp sensitive patients have identified a 34 kDa protein as the cross-reacting crustacean allergen. Using shrimp as a model system and Sa-II as a representative crustacean allergen, further studies were carried out to get an insight into the structural and molecular basis of allergenic cross-reactivity. The strategies adopted were, (1) to raise allergen specific anti-idiotypic antibodies and explore the possibility of using these anti-idiotypic antibodies as surrogate allergens for diagnosis of crustacea allergy and (2) to identify the IgE binding epitopes on the major shrimp allergen Sa-II, which may be shared by the 34 kDa allergen from the related crustaceans. In order to explore idiotypic, anti-idiotypic and anti-anti-idiotypic responses to Sa-II, Balb/c mice were immunized with affinity purified human idiotypic antibodies directed against the purified allergen. This resulted in the production of anti-idiotypic antibodies which were quantitated using rabbit idiotypic antibodies raised against the same allergen. The mouse anti-idiotypic antibodies recognized shrimp-specific human idiotypic antibodies of the IgE isotype from 18 of 20 individuals, and IgG antibodies from 14 of 20 shrimp sensitive patients. Immunization of Balb/ c mice with affinity purified, allergen-specific anti-idiotypic antibodies induced anti-allergen IgE and IgG responses in the absence of the allergen. The induction of anti-anti-idiotypic antibodies functionally identical to allergen-specific idiotypic antibodies confirmed that the anti-idiotypic antibodies generated, are indeed a mirror image of the allergen. The present study thus provides evidence that anti-idiotypic antibodies raised against allergen-specific idiotypic antibodies may substitute for the original allergen in the induction of allergen-specific idiotypic antibodies. The demonstration of shared idiotopes on IgG and IgE antibodies in the sera of shrimp sensitive patients supports the use of allergen-specific anti-idiotypic antibodies as surrogate allergens. These anti-anti-idiotypic antibodies not only recognized Sa-II, but also the 34 kDa allergen from prawn, lobster and crab. Cross-reactivity studies using polyclonal sera of shrimp sensitive patients and Sa-II anti-anti-idiotypic antibodies have attributed the allergenic cross-reactivity observed among the related crustaceans to the presence of highly conserved IgE binding epitopes on the 34 kDa crossreacting allergen from shrimp, crab, lobster and prawn. In order to identify the igE binding epitopes on Sa-11, it was subjected to limited tryptic digestion and the peptides were separated by reverse phase HPLC. Amino acid sequence analysis of these peptides and several other peptides generated by Asp N and Lys C treatment revealed an 861 homology with the muscle protein tropomyosin from the fruit fly Drosophila melanogaster, suggesting that the major shrimp allergen is tropomyosin. To establish that Sa-II is indeed tropomyosin, the latter was isolated from shrimp and its physicochemical and immunochemical properties were compared with those of Sa-II. Both tropomyosin and Sa-II had the same molecular mass and focused in the isoelectric pH range of 4.8-5.4. In the presence of 6 M urea, the mobility of both Sa-I1 and shrimp tropomyosin shifted to give an apparent molecular mass of 50 kDa, which is a characteristic property of tropomyosins. Shrimp tropomyosin bound to specific IgE antibodies in the sera of shrimp sensitive patients as assessed by competitive ELISA inhibition and immunoblot analysis. Tropomyosin, similar to Sa-I1 was subjected to limited tryptic digestion and the tryptic maps of both Sa-II and tropomyosin as obtained by reverse phase HPLC were found to be super imposable. Dot blot immunoassay and competitive ELISA inhibition assay using the sera of shrimp sensitive patients identified two peptides, 6 and 9 that exhibited allergenic activity. Both the peptides were purified to homogeneity and sequenced. Peptide 6 is a nonapeptide corresponding to the amino adds 153-161 and peptide 9 has 17 amino acids corresponding to the aminoacid residues 50-66. The peptides individually blocked upto 50% the binding of allergen-specific IgE to hropomyosin. Sa-II specific mouse anti-anti-idiotypic antibodies recognized not only tropomyosin, but also the two allergenic peptides, thus confirming that these peptides represent the major IgE binding epitopes. The IgG binding activity was found to be associated with peptides 6 and 9 as assessed by dot blot immunoassay using the sera of shrimp sensitive patients. Thus, it was found that both IgG and IgE binding epitopes on shrimp tropomyosin are identical. Tropomyosins from both phylogenetically related and unrelated species were assessed for allergenic activity using the sera of shrimp sensitive patients. It was found that allergenic activity was associated with tropomyosins from related crustaceans and from Drosophila melanogaster which shares 86% homology with shrimp tropornyosin. However, tropomyosins from totally unrelated species like yeast, chicken, bovine, rat, rabbit and human did not exhibit allergenic activity. A comparison of the amino acid sequence of shrimp tropomyosin in the region of IgE binding epitopes with the corresponding regions of bopomyosins from different species confirmed lack of allergenic cross-reactivity. The allergenic peptides 6 and 9 were able to inhibit the binding of tropomyosins from related crustaceans to shrimp tropomyosin-specific IgE antibodies to the same extent, confirming the presence of highly conserved IgE binding epitopes. It has been established for the first time that the major crustacean allergen is the heat stable muscle protein, tropomyosin, and extensive cross-reactivity between different members of crustacea is due to the presence of highly conserved IgE binding epitopes on tropomyosins from these sources. Thus, from the present study, information with respect to the amino acid sequence of tropomyosin and localization of its 1gE binding epitopes, could be used to design synthetic peptides corresponding to the B cell and T cell epitopes which would find application in the diagnosis and desensitization of individuals allergic to crustacea.

Texte remanié de: Proefschrift--Sociale wetenschappen--Rijksuniversiteit te Leiden, 1995. / Bibliogr. p. 301-312. Index.

Texte remanié de: Proefschrift--Vrije Universiteit te Amsterdam, 2001. / Résumé en anglais. Bibliogr. p. 395-402. Index.

Thesis (Ph. D.)--Ohio State University, 2008. / Title from first page of PDF file. Includes bibliographical references (p. 228-234).

Thesis (M.S.)--University of North Texas, Aug., 2009. / Title from title page display. Includes bibliographical references.

Texte remanié de : Thèse de doctorat : Lettres : Université Yaoundé 1 : 2002. / Titre provenant de l'écran d'accueil. Bibliogr. p. [315]-327. Notes bibliogr.