This thesis explores and evaluates the temperature measuring capabilities of a phase-sensitive optical time-domain reflectometer (φ-OTDR), which exploits Rayleigh backscattering in normal single mode optical fibers. The device is constructed and its setup explained, and a protocol for making temperature measurements with it is developed. Performance tests are made and the device is shown to achieve fully distributed temperature measurements on fibers hundreds of meters in length with a spatial resolution of 1 m and a temperature resolution of 0.1 K. In addition, the capabilities of the device to measure normal strain in the measurement fiber are tested using the same approach, albeit with less success. The device is capable of very precise measurements, making it very sensitive to the environmental conditions around the measuring fiber but also susceptible to disturbances. Some discussion is had on how to avoid or deal with these disturbances. Furthermore, the technique is shown to be able to run in conjunction with other φ-OTDR measurement techniques from the same device simultaneously. / Det här examensarbetet utforskar och utvärderar förmågorna att mäta temperatur hos en fas-känslig optisk tidsdomän-reflektometer (φ-OTDR), som utnyttjar bakåtriktad Rayleigh-spridning i vanliga optiska singelmodfibrer. Anordningen konstrueras och dess komponentstruktur förklaras, och ett protokoll tas fram för att utföra mätningar med den. Prestandatester utförs och anordningen visas kapabel att göra fullt distribuerade temperaturmätningar längs hundratals meter långa fibrer, med en rymdsupplösning på 1 m och en temperaturupplösning på 0.1 K. Dessutom testas förmågan att mäta normaltöjning hos testfibern med samma metod, dock med mindre framgång. Anordningen är väldigt känslig för förhållandena i omgivningen runt mätningsfibern, vilket gör den kapabel till mätningar med mycket hög precision, men också mottaglig för störningar. Lite diskussion hålls kring hur dessa störningar kan undvikas eller hanteras. Vidare visas att mätningstekniken kan köras samtidigt som andra φ-OTDR-baserade tekniker från samma anordning.

Ligand-gated ion channels play an important role in electrochemical signal transduction across diverse organisms, yet their structural and functional intricacies are not fully understood. Particularly lacking is the knowledge of their response to variations in pH, an aspect necessary for understanding their physiological relevance and potential therapeutic targeting in neurological diseases. In this thesis project, I have investigated the mechanistic response of sTeLIC, a recently reported prokaryotic member of the pentameric ligand-gated ion channel family, to different environmental conditions. Using molecular dynamics simulations, a total of 16 different environmental conditions have been explored including variations in pH (neutral and alkaline), the presence and absence of calcium, and the inclusion of an electric field acting as an external driving force on charged atoms. The results reveal a comprehensive pH-sensing and gating mechanism involving key residues, notably E106 (on the β6 strand) and E160 (on loop F), and their local microenvironments. Additionally, an inhibitory mechanism for calcium is proposed, with E160 playing an important role. The simulations including an electric field has provided support for a non-conventional ion pathway through the pore. Collectively, these results offer insights into a mechanistic framework that may extend to other physiologically relevant systems, providing a foundation for further investigations and potential future therapeutic intervention. / pLGICs har en viktig roll i det elektrokemiska signalsystemet i många organismer, men detaljerna i deras struktur och framför allt funktion är fortfarande inte helt klargjorda. Särskilt är detaljerna kring deras reaktion på ändringar i pH-värde relativt okända, vilket är en viktig del i att förstå kanalernas fysiologiska roll och för att potentiellt hitta läkemedel mot neurologiska sjukdomar där dessa är inblandade. I det här arbetet har jag undersökt hur sTeLIC, som är ett nyligen publicerat bakteriellt protein i familjen pLGICs, reagerar på olika ändringar i miljön. Jag har använt molekyldynamiksimuleringar för att unders öka 16 olika miljöer med två olika pH-värden (neutralt och alkaliskt), med eller utan kalcium samt med eller utan en extern drivkraft över membranet i form av ett elektrisk fält. Arbetet har resulterat i en föreslagen mekanism förhur sTeLIC känner av pH och hur öppningen av kanalen går till. Denna mekanism involverar aminosyrorna E106, som finns på β6-strängen, och E160, som finns på F-loopen, samt deras omgivning. Dessutom har en modulatorisk mekanism föreslagits för en kalciuminhiberande effekt på sTeLIC som också involverar E160. Simuleringarna med en drivkraft över membranet har gett stöd för en ny väg för joner genom kanalen. Tillsammans ger dessa resultat insikt i en mekanism som eventuellt kan appliceras p ̊a andra system. Detta har lagt grunden för fortsatt undersökning som potentiellt kan leda till framtida läkemedelsutveckling inom området.

<div>A growing global population and improved standard of living in developing countries have resulted in an unprecedented increase in energy demand over the past several decades. While renewable energy sources are increasing, a huge portion of energy is still converted into useful work using heat engines. The combustion process in diesel and petrol engines releases carbon dioxide and other greenhouse gases as an unwanted side-effect of the energy conversion process. By improving the efficiency of internal combustion engines, more chemical energy stored in petroleum resources can be realized as useful work and, therefore, reduce global emissions of greenhouse gases. This research focused on improving the thermal efficiency of opposed-piston engines, which, unlike traditional reciprocating engines, do not use a cylinder head. The cylinder head is a major source of heat loss in reciprocating engines. Therefore, the opposed-piston engine has the potential to improve overall engine efficiency relative to inline or V-configuration engines.</div><div><br></div>The objective of this research project was to further improve the design of opposed-piston engines by using computational fluid dynamics (CFD) modeling to optimize the engine geometry. The CFD method investigated the effect of intake port geometry and exhaust piston lead angle on the scavenging process and in-cylinder turbulence. After the CFD data was analyzed, scavenging efficiency was found insensitive to transfer port geometry and exhaust piston lead angle with a maximum change of 0.61%. Trapping efficiency was altered exclusively by exhaust piston lead angle and changed from 18% to 26% as the lead angle was increased. The in-cylinder turbulence parameters of the engine (normalized swirl circulation, normalized tumble circulation, and normalized TKE) experienced more complex relationships. All turbulence parameters were sensitive to changing transfer port geometry and exhaust piston lead angle. Some examples of trends seen during the analysis include: an increase in normalized swirl circulation from 0.01 to 4.45 due to changes in swirl angle, a change in normalized tumble circulation from -28.52 to 21.11 as swirl angle increased, and an increase in normalized tumble circulation from 14.20 to 33.68 as exhaust piston lead angle was increased. Based on the present work, an optimum configuration was identified for a swirl angle of 15°, a tilt angle of 10°, and an exhaust piston lead angle of 20°. Future work includes expanding the numerical model’s domain to support a complete cylinder-port configuration, adding combustion products to the diffusivity equation in the UDF, and running additional test cases to describe the entire input space for the sensitivity analysis.<br>