There were two purposes of this investigation: the first was to develop a method for studying spore germination, hyphal growth and mycelial development of the aquatic actinomycetes under the microscope so that the life cycle of this organism could be continuously followed. The second purpose was to determine the rate of carbon utilization from various types of nutritional sources, and to correlate this with the colony development and spore formation.

The purpose of this investigation is to determine if there was a metabolic relationship between the actinomycetes and the gram positive, spore-forming becilli in surface waters, and, if such a relationship was evident, to relate the association to the disappearances of typical actinomycete tastes and odors from waters.

The purpose of this investigation was an attempt to clarify the relatives taxonomic position of and to formulate some of the basic cyclic morphological and physiological processes occurring in an aquatic actinomycete possessing streptomycetal characteristics.