Soybeans (Glycine max L. Merr.) are a symbiont of two beneficial associations: biological nitrogen fixation (BNF) with Bradyrhizobium japonicum, and arbuscular mycorrhizal fungi (AMF). Within the Northern Great Plains of the USA, iron deficiency chlorosis (IDC) of soybean is a yield-limiting factor. The effects of IDC on BNF and AMF are not well defined. This study was conducted to determine the effects of IDC on BNF and AMF. A laboratory study was performed to compare three methods of measuring ureide-N, a product of BNF in soybeans. Field studies in soybean were performed at three locations at eastern N011h Dakota. The experimental design was a factorial combination of three cultivars and three treatments. The three cultivars, in order of decreasing chlorosis susceptibility, were NuTech NT-0886, Roughrider Genetics RG 607, and Syngenta S01-C9 RR. The three treatments were control, Sorghum bicolor L. companion crop planted with the soybean seed, and FeEDDHA applied with the soybean seed. Chlorosis severity was the greatest and least for the NuTech and Syngenta cultivars, respectively. The FeEDDHA treatment decreased chlorosis severity. Ureide levels were abnormally high in plants severely stunted by JDC. The excess accumulation of ureides in IDC-stunted plants suggests that plant growth was reduced more than the rate of nitrogen fixation. The AMF population \vas at an adequate level at all locations and not affected by cultivar or treatment, in general. In the laboratory study, the Patterson et al. method had greater ureide concentrations due to the non-specific measuring of ammonium compounds compared to the Vogels and Van der Drift and Goos methods. / North Dakota Soybean Council

Thesis (M.Sc.)--University of the Witwatersrand, Faculty of Science, School of Botany, 1991 / The vegetation of the Nylsvley Nature Reserve situated in the Northern Transvaal, South Africa. is a semi·arid savanna dominated by Burkea qfricana and Eragrostis paUensf interspersed with patches of Acacia s_pp.and E, ie/t(lnumni(.ma. The Butkea savanna is established on soil which is naturally low in phosphorus while the A,,;acia savanna is established on soU with higber phosphorus levels. Spnres of vesictdar~arbusculat mycorrhizal (VAM) fungi Were extracted from soil samples by wet 8i:¢ving and sucrose ("mttifugation after which they.· were enumerated .and identified. Thirteen VAM species were isolated from the savanna regions. The population was composed of three Gloltlus spp .• four .4cQulosp()ra spp., one .Gigaspora sp., three Scutel!ispora spp. and tWQ species of uncertain identity. Root samples were cleated. and stained with acidic glycerol·tcypan blue and assessed for mycoufMal colonization. Significant positive correlations were indicated. between spore densities ar.d mycorrhizal root infection. The total 81)01:'e popUlation was negntively correlated wiUlavailuble P, organic C, K. Ca. Mg, and pH. ~'tldividual specles differed markedly from the population as a whole in their bltemctions with.,soil facrots, these wem. examined using linear regressions. The VA;M root colonization as assessed b)! the mycorrhizal % WassigIlfijcant!y negativr1v: (forrelated with P white. the frequency % was. negatively correlated. Wilh P and K. .The inter-relatidhs!;tips 'between the d.ifferentVA1\i ,species and enviro~~eiita1 factors were further explored using prinCipal com'ponent ana,lyt;ls. the population structure and the factors affecting the population ate discussed. ..bltemctions with.,soil facrots, these wem. examined using linear regressions. The VA;M root colonization as assessed b)! the mycorrhizal % WassigIlfijcant!y negativr1v: (forrelated with P white. the frequency % was. negatively correlated. Wilh P and K. .The inter-relatidhs!;tips 'between the d.ifferentVA1\i ,species and enviro~~eiita1 factors were further explored using prinCipal com'ponent ana,lyt;ls. the population structure and the factors affecting the population ate discussed. / MN (2017)

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No description available.

Работа посвящена изучению микоризации и содержанию углерода и азота в тонких корнях травянистых растений на самозарастающих участках золоотвалов ВТГРЭС и СУГРЭС в сравнении с естественными сообществами. Встречаемость микоризы, корневых волосков и темных септированных эндофитов (ТСЭ) изучали методом световой микроскопии (Leica DM 5000B, Германия, × 100–200) после предварительной мацерации корней в КОН и окрашивания анилиновым голубым [Селиванов, 1981]. Содержание общего N и C определяли на CHNS-O анализаторе EURO EA-3000 CHN. Результаты исследований показали наличие арбускулярной микоризы с гифами, везикулами и арбускулами у всех изученных видов травянистых растений. Развитие микоризы, встречаемость ТСЭ и корневых волосков зависело от местообитания. Микориза менее развита у видов золоотвала СУГРЭС. На золоотвале ВТГРЭС у растений чаще встречались корневые волоски, а мицелий ТСЭ был лучше развит в корнях растений естественных сообществ. Анализ содержания углерода (С) и азота (N) в тонких корнях показал, что содержание N зависело от таксономического положения растения. Больше всего N в корнях бобовых растений. У небобовых двудольных N в корнях большем, чем у однодольных растений. Содержание N сильно варьировало в разных фитоценозах и у лесных видов N в корнях было выше, чем у луговых видов. Концентрация С в корнях зависела от местообитания и была выше у растений золоотвала ВТГРЭС и естественных сообществ по сравнению с золоотвалом СУГРЭС. Содержание N в тонких корнях сопоставимо на всех участках. Материал диссертации получен в результате работы научного коллектива в ходе выполнения проекта РФФИ (грант 18-04-00714). Магистрантом лично получены данные по микоризообразованию травянистых видов на золоотвале СУГРЭС, а также подготовлены пробы для анализа содержания С и N в тонких корнях растений. Данная выпускная работа содержит 35 страниц машинописного текста, включает 14 рисунков, 2 таблицы, 105 источников литературы, в том числе 57 иностранных. / The work is devoted to the study of mycorrhization and the content of carbon and nitrogen in the thin roots of herbaceous plants in the self-growing sections of VtSDPP and SuSDPP ash dumps in comparison with natural communities. The occurrence of mycorrhiza, root hairs, and dark septic endophytes (TSE) was studied by light microscopy (Leica DM 5000B, Germany, × 100–200) after preliminary maceration of the roots in KOH and staining with aniline blue [Selivanov, 1981]. The content of total N and C was determined on a CHNS-O analyzer EURO EA-3000 CHN. The results of the studies showed the presence of arbuscular mycorrhiza with hyphae, vesicles, and arbuscules in all studied species of herbaceous plants. The development of mycorrhiza, the occurrence of TSE and root hairs depended on the habitat. Mycorrhiza is less developed in species of the ash dump of SuSDPP. Root hairs were more common in plants at VtSDPP ash dump, and TSE mycelium was better developed in plant roots of natural communities. Analysis of the carbon (C) and nitrogen (N) content in thin roots showed that the N content depended on the taxonomic position of the plant. Most N in the roots of legumes. In non- leguminous dicotyledonous N, the roots are larger than in monocotyledonous plants. N content varied greatly in different phytocenoses and in forest species N in the roots was higher than in meadow species. The concentration of C in the roots depended on the habitat and was higher in plants of the VtSDPP ash dump and natural communities compared with the SuSDPP ash dump. The content of N in thin roots is comparable in all areas. The dissertation material was obtained as a result of the work of the research team during the implementation of the RFBR project (grant 18-04-00714). The graduate student personally obtained data on the mycorrhiza formation of herbaceous species in the ash dump of SUGRES, as well as prepared samples for analysis of the content of C and N in the thin roots of plants. This work contains 35 pages of typewritten text, includes 14 figures, 2 tables, 105 sources of literature.

Plant symbiosis with arbuscular mycorrhizal (AM) fungi provides many benefits for plants, including increased nutrient uptake, drought tolerance, and belowground pathogen resistance. In order to have a better understanding of the genetic architecture of mycorrhizal symbiosis, we conducted a genome-wide association study (GWAS) by phenotyping a diversity panel of cultivated sunflower (Helianthus annuus) for root colonization under inoculation with the AM fungus Rhizophagus intraradices. This mapping panel consists of 261 inbred lines that capture approximately 90% of the genetic diversity present in the cultivated sunflower germplasm. Using a mixed linear model approach with a high-density genetic map, we determined regions of the genome that are likely associated with AM colonization in sunflower. Additionally, we used a ‘core 12' set of twelve diverse lines (representing approximately 50% of the genetic diversity in the cultivated germplasm) to assess the effect that inoculation with AM fungi has on dried shoot biomass and macronutrient uptake. Colonization rate among lines in the mapping panel ranged from 0 to 70% and was not correlated with mycorrhizal growth response, shoot P response, or shoot K response among the core 12 lines. Association mapping yielded three SNPs that were significantly associated with AM colonization rate. These SNPs explained 19.0%, 14.4%, and 27.9% of the variance in three different metrics used to measure the degree of root colonization. Three genes of interest identified from the significant regions that contained these SNPs are potentially related to plant defense. Overall, our data suggests that candidate genes involved in plant defense may affect AM colonization rates within cultivated sunflower, and that these genes have a large effect size.

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The vast majority of terrestrial plant species live in symbiosis with arbuscular mycorrhizal fungi (AMF). AMF and plants live in complex networks, with roots of individual plants hosting multiple AMF, and single AMF colonizing multiple plants concurrently. Through the exchange of resources, the two partners of this symbiosis can have great effects on each other, effects which can ripple through both communities. What determines the patterns of associations between the partners is still largely unknown. In this dissertation, I examine a variety of factors, and in particular host identity, that could drive the community composition of AMF in roots. I began by surveying the diversity of AMF in roots of 12 plant species at a remnant bunchgrass prairie in Oregon, U.S.A. (Chapter 2). To do that, I first designed new primers for use in polymerase chain reaction (PCR) to specifically amplify DNA from all Glomeromycota species. Using those primers, I found 36 distinct AMF phylogenetic groups, or operational taxonomic units (OTUs) in the roots from the prairie. The proportion of OTUs in the basal order Archaeosporales was greater than in many other environmental surveys. I also conducted an in silico analysis to predict how effectively previously published primers would detect the whole diversity of OTUs I detected. I then assayed AMF community composition in the roots of 50 plants from nine plant species (Chapter 3). To do that, I designed primers specific to 18 of the OTUs detected in the initial field survey and used them to test for the presence of each OTU in the roots individual plants. I used that data to test if AMF community composition in individual roots correlated with host identity, spatial distribution, or soil characteristics. I found host identity was associated with both the richness and the structure of root AMF communities, while spatial distribution and soil characteristics were not. Finally, I performed an experimental test of the effect of host identity and community context on AMF community assembly (Chapter 4). I grew plants from four native perennial plant species, including two common and two federally endangered plants, either individually or in a community of four plants (with one plant of each species). I analyzed the AMF community composition in the roots of all plants after 12 weeks of growth with exposure to a uniform mix of field soil as inoculum. I found that host species identity affected root AMF richness and community composition, and community context affected AMF richness. Only one of the endangered species was highly colonized by AMF, and I did not detect unique AMF communities associated with it. This dissertation provides information on the diversity of AMF at a remnant bunchgrass prairie, an ecosystem which has been the subject of very few studies of AMF. Although a complex mix of factors interact to determine AMF community composition in roots, this work provides strong evidence that host identity plays a major role in that process. / Graduation date: 2013

Arbuscular mycorrhizal (AM) fungi are root and soil inhabiting symbionts with higher plants. The fungi are especially nutrient-function efficient in nutrient deficient soils. There have been innumerable studies of AM fungal facilitation of plant nutrient uptake in controlled environments. Comparatively little similar investigation has been undertaken in natural soils, including investigation of taxon specific nutrient-function efficiency in the phylum. Plant diversity and frequency, soil chemistry statuses, and AM spore diversity and abundance were sampled in an interrupted-belt transect in an aggrading dune sytem on west-coast India, followed by foredunes and transect nutrient amendment experiments in selected plant species. The transect extends 175 m inland from mean high-water mark (MH-WM). Examination showed nutrients were consistently deficient. A plant zonation pattern and increasing frequency over the transect were indicated, as well as decreasing pH and increasing organic matter (OM)-amendment AM species diversity gradients. Plant zonation does not correlate with soil chemistry. There was a distinct soil transition at the 175 m point and evidence of further system partition between foredune and behind-foredune regions. Plant and AM demographies bore no resemblance suggesting neither is driven by the other. Four AM genera were recovered, Acaulospora, Gigaspora and Scutellospora in high abundance, Glomus in comparatively low abundance. The two co-dominant species, A. spinosa and Gi. margarita, displayed divergent strategies in OM amendment. Certain AM taxa may be functionally associated with particular soil nutrients. There was no evidence of taxon-specific nutrient-function efficiency.

Thesis (MSc (Botany and Zoology)--University of Stellenbosch, 2006. / Parts of the West Coast Strandveld and adjacent Succulent Karoo on the arid coast of Namakwaland in the Western Cape of South Africa are subject to surface mining. An understanding of mycorrhizal association of plants in the natural vegetation of this area could contribute to the improvement of post-mining re-vegetation of the area. This study investigated mycorrhizal association of plants in the West Coast Strandveld, and compared mycorrhizal infection rates of soils taken from natural vegetation to soils from post-mined rehabilitated vegetations. The study was divided into two components. In the first component a pot experiment was conducted in the greenhouse to assess vesicular-arbuscular mycorrhiza (AM) infectivity of post-mined rehabilitated areas of Brand se Baai in Namakwa Sands mining areas. Rehabilitated areas used in this study included sites that has been strip mined for heavy minerals and then progressively backfilled with sub-soil sand remaining after mineral extraction (tailings), topsoil and translocated plants in an effort to restore the structure and functional aspects of the mined site to its original (pre-mining) ecosystem. Rehabilitated sites 1 assessed in this study included sites backfilled with: tailings + translocated plants (TP); tailing + topsoil + translocated plants (TSP) and tailings + topsoil only (TS). Natural sites (N) were also assessed to serve as reference points. AM infection was evaluated as percent root colonization on wheat planted as bioassay on sterilised sand and inoculum from rehabilitated sites in the ratio of 3:1 respectively. Results of this study component showed that mycorrhiza infectivity of rehabilitated soils was high on TSP and TS because mining disturbance has been remedied by topsoil with or without translocated plant replacement. The structural and chemical components of topsoil used as rehabilitation material favoured re-establishment of microbial activities. Infectivity was however low on soils rehabilitated with tailings and translocated plants (TP) because this treatment lacked topsoil which is a major source of infective mycorrhizal propagules. Infectivity was also low in soils from undisturbed sites (N) probably high phosphorus concentration or presence of perennial vegetation led to low mycorrhiza infection. Results showed that there was no significant effect of mycorrhiza on plant growth rate, nutrient uptake or carbon cost of mycorrhizal plants when related to non-mycorrhizal plants, instead the biomass production and nutrient contents of plants were determined by chemical properties of treatment soils. The second component of the study investigated presence of mycorrhiza on randomly selected common indigenous species of Aizoaceae, Asparagaceae, Asteraceae, Chenopodiaceae, Fabaceae, Lamiaceae, Mesembryanthemaceae, Restionaceae, families growing on unmined areas of the study site. Total mycorrhiza infection was recorded on 85% of the assessed species with percent infection level ranging from 8% in Atriplex lindleyi and Drosanthemum hispidum to 98% in Salvia lanceolata. Functional mycorrhizal association with arbuscule structures were however only observed on 15% of all species assessed. Low arbuscules infection observed in indigenous species assessed in this study could be associated with the timing of mycorrhiza infection assessment and root competition in the soil. There was no infection observed on four species belonging to Chenopodiaceae, Zygophyllaceae, Sterculiaceae, and Asteraceae families, which represented 15% of all species assessed. Most species belonging to Chenopodiaceae and Zygophyllaceae have been reported as non-mycorrhizal in other studies, absence of mycorrhiza on the remaining three families species observed in this study require further confirmation.