Carotenoid utilization in rainbow trout (Oncorhynchus mykiss Walbaum) and Atlantic salmon (Salmo salar L.) has been investigated with respect to tissue distribution of carotenoids and the role of the liver on the bioavailability of the lipid soluble carotenoids, astaxanthin and canthaxanthin. Species-specific and tissue-specific accumulations were noted for astaxanthin and canthaxanthin in the rainbow trout and Atlantic salmon, possibly indicating fundamental differences in their utilization in these species. The liver and the kidney were revealed to be the major tissues involved in carotenoid metabolism in both rainbow trout and Atlantic salmon. Apparent digestibilities (-96% and -30% for rainbow trout and Atlantic salmon, respectively) and flesh carotenoid retentions (-12% and -5.4% for rainbow trout and Atlantic salmon, respectively) differed significantly between species, suggesting that rainbow trout are more efficient depositors of carotenoids within the flesh. Isolated rainbow trout liver perfusion experiments revealed small differences in the uptake of astaxanthin and canthaxanthin. Uptake of astaxanthin in both synthetically-derived and serum-derived models showed saturable uptake mechanism that occurred earlier than for canthaxanthin. These results can potentially offer an explanation for the better utilization of astaxanthin in rainbow trout, where the liver reduces the bioavailability of canthaxanthin through continued uptake. Results show a low hepatic extraction ratio (0.03-0.07), in line with published post-prandial elimination rates. Neither astaxanthin nor canthaxanthin significantly induced hepatic or renal xenobiotic-metabolizing enzymes in the rainbow trout, contrary to published reports in rats and mice. This may imply fundamental species-specific differences in the metabolic pathways for these carotenoids. Histochemical investigations revealed that both carotenoids significantly impacted liver structure, resulting in higher levels of total lipids and mucopolysaccharides. This is thought to be due to their antioxidant functions and their provitamin A activity. Carotenoid-treated fish also had higher levels of glycogen phosphorylase in liver sections, providing the first evidence in fish for the possibility of glucuronidation of their metabolites. The present investigations demonstrate the liver to be a major organ in carotenoid metabolism, and consequently affects carotenoid distribution and availability. In addition, carotenoid supplementation significantly affects liver structure and may potentially enhance its function. Furthermore, these investigations have provided new avenues of investigation into the use of isolated organ perfusions for biochemical nutrition research, and expanded the knowledge of liver physiology and biochemistry.

No description available.

The first part of this study investigated the effects of varying temperature regimes within the range of 8-22°C on the development and survival of Atlantic salmon (Salmo salar L.) eggs and alevins. The temperature tolerance of eggs was lower than that of alevins: egg mortality increased above 11°C and no eggs survived to eyeing or to hatching at 16 and 14°C, respectively; alevin mortality increased above 16°C and no alevins survived at 22°C. Optimal survivals of eggs and alevins occurred at 8-11°C and 10-14°C, respectively. Subsequent survival at later stages of development was largely determined by survival at earlier stages. Developmental abnormalities among eggs (pin-eyed eggs) and alevins (abnormal hatching and yolk-sac oedema) appeared to be temperature-dependent. Development time in days from fertilisation to eyeing, hatching and maximum alevin wet weight (MAWW) varied inversely with temperature. The sum of degree-days from fertilisation to eyeing and to MAWW was similar at all temperatures, but declined with increasing temperature from eyeing to hatching. The hatching period was similar for all temperatures except 8°C where it was significantly longer. Although alevin size at hatching was not temperature-dependent within the range of 8-12°C, alevin size at MAWW decreased progressively with increasing temperature (10-20°C) during the alevin stage. Fry size at first feeding did not affect their subsequent growth rate or survival. Advanced fry which were fed earliest grew at similar rates to those produced at lower temperatures and attained the greatest weight. Biomass gain was more dependent upon survival than upon mean fish weight. The second part of this study investigated the effects of timing of first feeding on fry growth and survival. Alevins fed prior to final yolk resorption were larger and had lower mortalities than those fed after MAWW. Although the "window" of first feeding opportunity lasted several weeks, delaying feeding beyond MAWW reduced absolute growth. A 5-week delay led to mortalities approaching 60%. However, first feeding can be delayed beyond MAWW for 1-2 weeks at 10°C without adversely affecting subsequent survival or growth rate.

In order to understand and predict climate in a world driven by anthropogenic influences, increased understanding of natural climate variability is vital. The doctoral dissertation presented here focuses on multi-decadal climate modes, and in particular, the Atlantic Multi-decadal Oscillation (AMO), and how it is expressed in the tropical to sub-tropical western Atlantic Ocean over the last ~700 years. Multi-decadal modes have been linked to weather around the Atlantic, and have also been correlated with the occurrence of anomalous climatic events (in both temperature and precipitation), although the relationship of these modes to anthropogenic impacts is still undefined. The study area for this project encompasses South Florida, the Bahamas, and the Caribbean; however, the dissertation begins with a review article covering the current published records and reconstructions of multi-decadal variability from the Atlantic sector. Beyond this, the research turns to the reconstruction of multi-decadal variability from biogenic carbonates, including corals and sclerosponges. Because Atlantic Multi-decadal Variability (AMV) is still poorly understood and current reconstructions diverge beyond the instrumental period, the use of long-lived coral and sclerosponge specimens allows for an examination of multi-decadal variability over the last several centuries. Two multi-century records of temperature and salinity are reconstructed from the geochemistry of a coral from coastal Florida and sclerosponge collected in the Bahamas. Additional analyses from corals collected throughout the Lesser Antilles are also examined to determine their sensitivity to Atlantic AMV for future work. The final chapter of this dissertation presents a study investigating the reproducibility and fidelity of some of the geochemical proxy techniques utilized in biogenic carbonates.

Hydrographic data, in the time range from 1908 to 2006, extracted from the International Council for the Exploration of the Sea (ICES) database, are used to examine variability of water masses in the North Atlantic ocean along 38N. All the data are interpolated in an ideal isopycnal framework, which has a longitudinal resolution of 1/3 degree and 50 isopycnal layers of variable thickness, to 38N by using an objective analysis approach. A 5 year-running mean triad analysis is performed from 1950 to 2004 for further variability study. Extensive decadal to inter-decadal variability is observed, in both shallow and deep layers. In the deep layers, a signal of westward phase propagating is detected, coincided with the time scale of a first mode baroclinic Rossby wave transporting at this latitude. Strong negative correlations (maximum at a lag of 7 years) are seen between the variability in the DWBC and the North Atlantic Oscillation. A similar correlation at the same lag is also detected in the basin interior, suggesting the Labrador Sea Water (LSW) pathway is not only restrained to the DWBC.

Graduation date: 1999

Diss. Stockholm : Stockholms universitet, 2007.

Thesis (Ph.D.) -- McMaster University, 2001. / Includes bibliographical references. Also available via World Wide Web,

Thesis (M.S. - Hydrology and Water Resources)--University of Arizona. / Includes bibliographical references (leaves 175-176).

Thesis (D. Min.)--Gordon-Conwell Theological Seminary, 2006. / Includes bibliographical references (leaves 55-56).