The grape flavonoids include anthocyanins, tannins and flavonols, all of which contribute to grape and wine quality by influencing the colour and mouthfeel of red wine. These compounds are synthesized in different parts of the berry and during different stages of berry development. In addition, environmental and viticultural factors such as light exposure can also alter the flavonoid composition of grapes. An understanding of how synthesis of these compounds is coordinated, their relationship to wine quality and the influence of bunch light exposure on the flavonoid composition of grapes, could be used to improve fruit quality by enhanced viticultural management. The first part of this study sought to investigate the relationship between the different products of the flavonoid biosynthetic pathway (anthocyanins, flavonols and tannins), from two climatic regions (warm and cool) and determine their role in grape and wine quality. In collaboration with a major winery, whole Shiraz grapes were sampled at the weighbridge from a range of different vineyards from two climatic regions; warm (Riverland) and cool (McLaren Vale) in 2003 and 2004. A total of 80 grape samples were collected in each season and processed (i.e. 100 berries, separated into skin, seeds and juice, weighed and frozen). Anthocyanins and flavonols were measured, in triplicate, in skins by HPLC. Tannins were determined in the skins and seeds by two methods; phloroglucinol hydrolysis (HPLC) and protein precipitation (UV-VIS spectrophotometer). A comprehensive comparison of the two methods is discussed. In both years, the grapes from warm and cool climates formed two distinct data sets based on flavonoid composition. There was a correlation between anthocyanins and flavonols for both the warm and cool climate samples in both years, however those from the warm region had lower anthocyanin for a given level of flavonol. As expected, the level of tannin in the seeds was greater than in skin for all samples. In both years, there was a weak correlation between anthocyanin levels in the skin and skin tannins, but no relationship with seed tannins. These results suggest there is some co-ordination in the synthesis of anthocyanins, flavonols and skin tannins. Also, the two regions clearly separated based on yield and despite the weak correlations in both regions, the levels of total anthocyanins were inversely related to yield. In addition, there was no relationship with any of the flavonoids and grape quality, indicating the need for improvement in streaming fruit for quality using these flavonoid compounds. The second part of the study was to investigate the effect of bunch light exposure on flavonol synthesis and accumulation in Shiraz and Chardonnay grapes during development. Light-excluding boxes were applied to bunches at budburst. Boxes were removed at four sampling times; flowering, pre-veraison, veraison and harvest. At each sampling time, berry skins were sampled when the boxes were removed and then every second day (light induced), along with exposed controls for one week. Flavonol accumulation and flavonol synthase (VvFLS1) gene expression was determined by HPLC and Real Time-PCR (RT-PCR) respectively. As expected, for all four sampling times, flavonol accumulation and VvFLS1 expression in the boxed fruit was significantly less than bunches exposed to light. On removal of boxes at flowering, pre-veraison and veraison, flavonols accumulated to levels similar to that of the exposed control fruit over a period of 4-6 days. There was a significant increase in VvFLS1 expression 2 days after exposure to light in parallel with the accumulation of flavonols. At harvest, in Chardonnay, VvFLS1 expression peaked by day 4, while in Shiraz VvFLS1 expression increased linearly and was highest at day 6. In contrast to the results for the earlier sampling times, the total amount of flavonols accumulated at harvest was less than 50% of exposed controls in Chardonnay and Shiraz grapes. These results show that flavonols are able to be induced by bunch light exposure at different times during berry development, including times when flavonols are not normally being synthesised. This suggests bunch light exposure can override the developmental control of flavonol accumulation. To further investigate the light induced expression of VvFLS1 in grapevines the molecular mechanism of transcriptional control was explored. Using genomic walking PCR techniques, two Shiraz VvFLS1 promoter sequences were cloned and their sequences were analysed. These promoter sequences were ~800bp in length and were 99% identical. A putative MYB responsive element (MRE) and several light responsive elements (LRE) were identified in the promoter region of these genes. To functionally test the VvFLS1 promoter(s), a transient assay was developed in Chardonnay suspension cells. Cells were bombarded with constructs containing potential transcription factors and the VvFLS1 promoter(s), fused to a luciferase reporter vector. After 48hrs incubation in the dark, cells were harvested and luciferase activity measured as an indicator of VvFLS1 promoter activity. Of the different transcription factors tested with the VvFLS1 promoter(s) the highest luciferase activity was observed using AtMYB12 (a flavonol-specific regulator of AtFLS1 in Arabidopsis (Mehrtens et al. 2005). While this result shows activation of the VvFLS1 promoters by AtMYB12 and the development of a transient reporter assay for testing the VvFLS1 promoter(s) a grapevine transcription factor specific for VvFLS1 was sought. Two techniques were employed to identify potential transcription factor regulators of the VvFLS1 promoter(s). The first involved BLAST sequence search analysis in a grapevine expression (EST) database with AtMYB12 and the second involved using DNA microarray technology to identify candidate transcription factors that were up-regulated in light exposed Chardonnay cell suspension cultures. Thirteen potential transcription factors were identified and after correlative RT-PCR analysis (with VvFLS1 expression patterns) two candidates were selected for further isolation and characterisation. These results have made significant progress in unravelling the molecular mechanisms of regulation of the flavonol biosynthetic, however additional experiments are required to unravel the transcriptional control of flavonol biosynthesis. This investigation contributes to our knowledge of flavonoid synthesis in grapes; how it is coordinated, the relationship with wine quality, and the influence of light particularly on synthesis of flavonols. It also explores the molecular mechanisms of VvFLS1 control, through isolation of the VvFLS1 promoter and identification of potential transcription factors, which may regulate it. An understanding of the synthesis of flavonoids and how they may be coordinated, particularly in response to light, could be used to improve fruit quality by enhanced viticultural management. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1326767 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

Thesis (M. Env. St.)--University of Adelaide, Mawson Graduate Centre for Environmental Studies, 1994? / Includes bibliographical references (leaves 74-84).

Thesis (M.S.)--Michigan State University. Dept. of Entomology, 2006. / Title from PDF t.p. (viewed on June 19, 2009) Includes bibliographical references (p. 106-117). Also issued in print.

Thesis (Ph. D.)--University of Oregon, 1999. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 356-371). Also available for download via the World Wide Web; free to University of Oregon users. Address: http://wwwlib.umi.com/cr/uoregon/fullcit?p9947971.

Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: Grape quality is linked to the organoleptic properties of grapes, raisins and wine. Many advances have been made in understanding the grape components that are important in the quality of wines and other grape products. A better understanding of the compositional content of grapes entails knowing when and how the various components accumulate in the berry. Therefore, an appreciation of grape berry development is vitally important towards the understanding of how vineyard practices can be used to improve the quality of grapes and eventually, wines. The more established methods for grape berry quality assessment are based on gravimetric methods such as colorimetry, fluorescence and chromatography. These conventional methods are accurate at targeting particular components, but are typically multi-step, destructive, expensive, polluting procedures that might be technically challenging. Very often grape berries are evaluated for quality (only) at harvest. This remains a necessary exercise as it helps viticulturists and oenologists to estimate some targeted metabolite profiles that are known to greatly influence chemical and sensory profiles of wines. However, a more objective measurement of predicting grape berry quality would involve evaluation of the grapes throughout the entire development and maturation cycle right from the early fruit to the ripe fruit. To achieve this objective, the modern grape and wine industry needs rapid, reliable, simpler and cost effective methods to profile berry development. By the turn of the last millennium, developments in infrared instrumentation such as Fourier-transform infrared (FT NIR) and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR) in combination with chemometrics resulted in the development of rapid methods for evaluating the internal and external characteristics of fresh fruit, including grapes. The advancement and application of these rapid techniques to fingerprint grape compositional traits would be useful in monitoring grape berry quality. In this project an evaluation of grape berry development was investigated in a South African vineyard setting. To achieve this goal, Sauvignon blanc grape berry samples were collected and characterised at five defined stages of development: green, pre-véraison, véraison, post-véraison and ripe. Metabolically inactivated (frozen in liquid nitrogen and stored at -80oC) and fresh berries were analysed with FT-IR spectroscopy in the near infrared (NIR) and mid-infrared (MIR) ranges to provide spectral data. The spectral data were used to provide qualitative (developmental stage) and quantitative (metabolite concentration of key primary metabolites) information of the berries. High performance liquid chromatography (HPLC) was used to separate and quantify glucose, fructose, tartaric acid, malic acid and succinic acid which provided the reference data needed for quantitative analysis of the spectra. Unsupervised and supervised multivariate analyses were sequentially performed on various data blocks obtained by spectroscopy to construct qualitative and quantitative models that were used to characterise the berries. Successful treatment of data by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) gave statistically significant chemometric models that discriminated the berries according to their stages of development. The loadings from MIR models highlighted the important discriminant variables responsible for the observed developmental stage classification. The best calibration models to predict metabolite concentrations were obtained from MIR spectra for glucose, fructose, tartaric acid and malic acid. The results showed that both NIR and MIR spectra in combination with multivariate analysis could be reliably used to evaluate Sauvignon blanc grape berry quality throughout the fruit’s development cycle. Moreover, the methods used were fast and required minimal sample processing and no metabolite extractions with organic solvent. In addition, the individual major sugar and organic acids were accurately predicted at the five stages under investigation. This study provides further proof that IR technologies are robust and suitable to explore high-throughput and in-field application of grape compound profiling. / AFRIKAANSE OPSOMMING: Druifkwaliteit word gekoppel aan die organoleptiese eienskappe van druiwe, rosyntjies en wyn. Baie vooruitgang is reeds gemaak in die begrip van druifkomponente wat belangrik is vir die kwaliteit van wyn en ander druifprodukte. ’n Beter begrip van die samestellende inhoud van druiwe behels om te weet wanneer en hoe die verskeie komponente in die korrel opgaar. ’n Evaluasie van druiwekorrel-ontwikkeling is dus uiters belangrik vir ’n begrip van hoe wingerdpraktyke gebruik kan word om die kwaliteit van druiwe, en uiteindelik van wyne, te verbeter. Die meer gevestigde maniere vir die assessering van druiwekorrelkwaliteit is gebaseer op gravimetriese metodes soos kolorimetrie, fluoressensie en chromatografie. Hierdie konvensionele metodes is akkuraat om spesifieke komponente te teiken, maar behels tipies veelvuldige stappe en is prosesse wat destruktief en duur is, besoedeling veroorsaak, asook moontlik tegnies uitdagend is. In baie gevalle word druiwekorrels (eers) tydens oes vir kwaliteit geëvalueer. Hierdie is steeds ’n noodsaaklike oefening omdat dit wingerdkundiges en wynkundiges help om die metabolietprofiele wat daarvoor bekend is om ’n groot invloed op die chemiese en sensoriese profiele van wyn te hê en dus geteiken word, te skat. ’n Meer objektiewe meting om druiwekorrelkwaliteit te voorspel, sou die evaluering van die druiwe dwarsdeur hulle ontwikkeling- en rypwordingsiklus behels, vanaf die vroeë vrugte tot die ryp vrugte. Om hierdie doelwit te behaal, benodig die moderne druiwe- en wynbedryf vinnige, betroubare, eenvoudiger en kostedoeltreffende metodes om ’n profiel saam te stel van korrelontwikkeling. Aan die einde van die vorige millennium het ontwikkelings in infrarooi instrumentering soos Fourier-transform infrarooi (FT NIR) en attenuated total reflectance Fourier-transform infrarooi spektroskopie (ATR FT-IR) in kombinasie met chemometrika gelei tot die ontwikkeling van vinnige metodes om die interne en eksterne kenmerke van vars vrugte, insluitend druiwe, te meet. Die vooruitgang en toepassing van hierdie vinnige tegnieke om ‘vingerafdrukke’ te bekom van die samestellende kenmerke sal nuttig wees vir die verbetering van druiwekorrelkwaliteit. In hierdie projek is ’n evaluering van druiwekorrelontwikkeling in ’n Suid-Afrikaanse wingerdligging ondersoek. Ten einde hierdie doel te bereik, is Sauvignon blanc druiwekorrelmonsters op vyf gedefinieerde stadiums van ontwikkeling versamel en gekarakteriseer: groen, voor deurslaan, deurslaan, ná deurslaan en ryp. Metabolies geïnaktiveerde (bevrore in vloeibare stikstof en gestoor teen -80oC) en vars korrels is met FT-IR spektroskopie in die naby infrarooi (NIR) and mid-infrarooi (MIR) grense geanaliseer om spektrale data te verskaf. Die spektrale data is gebruik om kwalitatiewe (ontwikkelingstadium) en kwantitatiewe (metabolietkonsentrasie van belangrikste primêre metaboliete) inligting van die korrels te verskaf. High performance liquid chromatography (HPLC) is gebruik om glukose, fruktose, wynsteensuur, appelsuur en suksiensuur te skei en te kwantifiseer, wat die verwysingsdata verskaf het wat vir die kwantitatiewe analise van die spektra benodig word. Ongekontroleerde en gekontroleerde meervariantanalises is opeenvolgend op verskeie datablokke uitgevoer wat met spektroskopie verkry is om kwalitatiewe en kwantitatiewe modelle te verkry wat gebruik is om die korrels te karakteriseer. Suksesvolle behandeling van die data deur hoofkomponent analise (principal component analysis (PCA)) en ortogonale parsiële kleinstekwadraat diskriminant analise (partial least squares discriminant analysis (OPLS-DA)) het statisties betekenisvolle chemometriese modelle verskaf wat die korrels op grond van hulle ontwikkelingstadia onderskei het. Die ladings vanaf die MIR-modelle het die belangrike diskriminantveranderlikes beklemtoon wat vir die klassifikasie van die waargenome ontwikkelingstadium verantwoordelik is. Die beste kalibrasiemodelle om metabolietkonsentrasies te verkry, is vanuit die MIR-spektra vir glukose, fruktose, wynsteensuur en appelsuur bekom. Die resultate toon dat beide die NIR- en MIR-spektra, in kombinasie met meervariantanalise, betroubaar gebruik kan word om Sauvignon blanc druiwekorrelkwaliteit dwarsdeur die vrug se ontwikkelingsiklus te evalueer. Verder is die metodes wat gebruik word, vinnig en het hulle minimale monsterprosessering en geen metabolietekstraksies met organiese oplosmiddel benodig nie. Daarbenewens is die vernaamste suiker en organiese sure individueel akkuraat voorspel op die vyf stadia wat ondersoek is. Hierdie studie verskaf verdere bewys dat IR-tegnologieë robuus en geskik is om hoë-deurset en in-veld toepassings van profielsamestelling van druiweverbindings te ondersoek.

This thesis examines how Gwyn Thomas and Ron Berry interact with and respond to landscape and environment in their fictional and non-fictional writing. Exploring how the writers negotiate the convergence of the industrial and the rural/natural in the uplands of the south Wales coalfield, in particular the Rhondda Fawr Valley, the study considers the literary geographies their work creates. Examining the themes of the cultural and political use of landscape and rural imagery, the manifestation of authority in landscapes, the impact of industrialisation and de-industrialisation, the uncanny underground environment and its dynamic interactions with the ground above, and post-industrial environmental issues, the study re-positions two industrial writers of Wales to reveal the significance of landscape, place and environment in their writing.

Submitted by Alison Vanceto (alison-vanceto@hotmail.com) on 2016-10-14T12:48:52Z No. of bitstreams: 1 TeseVLON.pdf: 12327358 bytes, checksum: 6fe55d85e4aff3ba5318f9671501f27c (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-21T13:10:16Z (GMT) No. of bitstreams: 1 TeseVLON.pdf: 12327358 bytes, checksum: 6fe55d85e4aff3ba5318f9671501f27c (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-21T13:10:23Z (GMT) No. of bitstreams: 1 TeseVLON.pdf: 12327358 bytes, checksum: 6fe55d85e4aff3ba5318f9671501f27c (MD5) / Made available in DSpace on 2016-10-21T13:10:31Z (GMT). No. of bitstreams: 1 TeseVLON.pdf: 12327358 bytes, checksum: 6fe55d85e4aff3ba5318f9671501f27c (MD5) Previous issue date: 2016-03-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / In this thesis, the electronic and structural properties of nanostructured systems were studied aiming to get a realistic model for quantum rings, potentially adaptable for quantum dots. To attain these goals, several studies supported by experimental results were carried out that allowed the introduction to the building blocks for the theoretical models such as: the envelope function approach within the k.p approximation in quantum wells, and quantum ring/dot with perpendicular magnetic field and without spin-orbit interaction. In these models, the effects of size, strain and localization were subsequently introduced to understand the ring formation process and their effects in the photoluminescence and magneto-photoluminescence. The experimental results of atomic force microscopy indicated the importance of structural properties and the types of asymmetries possibly found in quantum rings after the growth process. The understanding of these effects and the evidence of the anisotropy in a preferential direction of the ring helped building more realistic models for the potential profiles. Various systems were then studied with success. They also included a controllably magnetic field (both in magnitude and orientation), beside the geometric deformation, making the ring ellipsoidal, and taking into account the spin-orbit interaction. The most realistic model was used to analyze the Berry phase generation and the relative weight of the contribution of each term of the Hamiltonian. / Nesta tese de doutorado, as propriedades eletrônicas e estruturais de sistemas nano estruturados foram estudadas visando a obtenção de um modelo realístico para anéis quânticos potencialmente adaptáveis a pontos quânticos. Para alcançar este objetivo, foram feitos alguns estudos, apoiados por resultados experimentais, que permitiram a construção passo a passo do modelo teórico, como: aproximação da função envelope na representação k.p em poços quânticos e anéis/pontos quânticos com campo magnético perpendicular e sem interação spin-órbita. Nestes modelos, os efeitos do tamanho, tensão e localização foram introduzidos subsequentemente para entender o processo de formação do anel e os resultados apresentados na fotoluminescência e na magneto-luminescência. O resultado experimental da microscopia de força atômica nos levou a analisar a importância das propriedades estruturais e os tipos possíveis de assimetria encontrados em anéis quânticos devido ao processo de crescimento. O entendimento desses efeitos e a evidência de anisotropia em uma direção preferencial do anel ajudou na construção de modelos mais realísticos para os perfis de potencial. Deste modo, vários sistemas foram estudados com sucesso. Eles também possuíam um campo magnético controlável (ambas, magnitude e orientação), além da deformação geométrica, que torna o anel elipsoidal, e a interação spin-órbita. O modelo mais realístico foi usado para analisar a geração da fase de Berry e o peso da contribuição de cada termo do Hamiltoniano.

Submitted by Vasti Diniz (vastijpa@hotmail.com) on 2017-09-18T14:16:14Z No. of bitstreams: 1 arquivototal.pdf: 2655836 bytes, checksum: 6306e048c6d679513585a208ceaa9e95 (MD5) / Made available in DSpace on 2017-09-18T14:16:14Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2655836 bytes, checksum: 6306e048c6d679513585a208ceaa9e95 (MD5) Previous issue date: 2013-03-15 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Recently, Mesaros, Sadri and Zaanen investigated the rise of Berry phases in the dynamics of quasiparticle in graphene with edge dislocation. In opposition with disclina­tions, dislocations require only finites energies to be created so that is virtually impossible to prepare one crystal, which doesn't have dislocations. Mesaros, Sadri e Zaanen used the theory of classic elasticity, to introduce informations due deslocations, in the Hamiltonian of particle and also used tigth-binding method to describe the system. They obtained that dynamics particle acquires one Berry phase and which this phase can be used at applications in quantic computation. In this work, we use the Katanev and Volovich geometric theory of defects to introduce dislocations in the graphene's sheet. We obtain the metric which descibe edge dislocations. We obtain the Hamiltonian which descibe the dynamic of quasiparticle in the graphene at curved space-time with torsion. Write the Dirac equation to this system and investigate the rise of Berry phase in this system. We show that Berry phase obtained to our system depends of intensity of Burgers vector. / Recentemente, Mesaros, Sadri e Zaanen investigaram o aparecimento de fases de Berry na dinamica de quasiparticulas em grafeno com deslocagoes tipo edge. Em contraste com desclinagoes, as deslocagoes requerem apenas energias finitas para serem criadas, de modo que é virtualmente impossivel preparar um cristal que nao contem deslocagoes. Mesaros, Sadri e Zaanen usaram a teoria da elasticidade classica, para introduzir as infor­magoes devido a deslocacao, no hamiltoniano da particula e usaram metodo tight-binding numa aproximagao de continuo para descrever o sistema. Eles obtiveram que a dinamica da particula adquire uma fase de Berry e que esta fase pode ser usadas para aplicagoes em computagao quantica. Neste trabalho, usamos a teoria geometrica de defeitos de Katanaev e Volovich para introduzir deslocagoes em uma folha de grafeno em uma aproximagao de continuo. Obtemos a metrica que descreve uma deslocagao tipo edge. Obtemos o hamil­toniano que descreve a dinamica das quasiparticulas no grafeno neste espago curvo com torgao. Escrevemos a equagao de Dirac para esse sistemas e investigamos o aparecimento de fases de Berry neste sistema. Mostramos que a fase geometrica obtida para o nosso sistema depende da intensidade do vetor de Burgers.

A idade das fêmeas colonizadoras de Hypothenemus hampei (Ferrari) durante o abandonamento do hospedeiro, assim como sua resposta aos voláteis de flores de café, Coffea arabica L. var. Tupi, foram investigadas neste trabalho. Um dispositivo experimental que simulasse as condições de dentro do fruto de café e permitisse a observação da saída dos insetos foi utilizado para a determinação da idade das fêmeas colonizadoras. Em média, os besouros apresentaram 15 dias de idade no momento em que abandonaram o dispositivo experimental. Nesta idade as fêmeas estavam acasaladas, possuiam o tegumento totalmente melanizado, foram capazes de voar e produzir ovos viáveis. Os voláteis de flores de café, foram coletados por aeração, analisados por GC-EAD e GC-MS, e testados em bioensaios olfatométricos. As fêmeas colonizadoras de H. hampei foram atraídas pelos voláteis de flores de café. Dos 50 compostos encontrados nos extratos naturais, sete foram eletrofisiologicamente ativos aos insetos. Destes sete, somente metil salicilato, neral e geranial puderam ser identificados, e a mistura de seus padrões sintéticos foi a mais atrativa nos bioensaios olfatométricos. Tomados juntos, estes resultados trazem novas informações sobre a bioecologia e ecologia química da broca-do-café, as quais poderão ser usadas futuramente em pesquisas de base e naquelas focadas no desenvolvimento de novas estratégias de manejo para H. hampei em cafezais. / The age of Hypothenemus hampei (Ferrari) females during the host abandonment, as well as their response to coffee flower volatiles were determinated under laboratory conditions. A experimental device that simulated the conditions inside a coffee berry, and that allowed the observation the insects coming out was used to determinate the age of colonizing females. On average, the beetles were 15 d-old at the moment they abandoned the experimental device. In this age, the females were mated, had a fully melanized tegument, were able to fly and to produce viable eggs. The volatiles of coffee, Coffea arabica L. var. Tupi, flowers were colected by aeration, analyzed in GC-EAD and GC-MS, and tested in olfactometer bioassays. The colonizing H. hampei females were attracted by the coffee flower volatiles. Of 50 compounds found in the natural extracts, seven were electrophysiologically actives to the insects. Of these seven, only methyl salicylate, neral and geranial could be identified, and the blend with their syntetic standards was the most atractive in the olfactometer bioassays. Taken together, these results bring new information on the bioecology and chemical ecology of the coffee berry borer, which may be used in the future by basic researchs or by those focused on the development of new strategies for the management of H. hampei in coffee farms.

O estudo da distribuição de pragas em espaço e tempo em sistemas agrícolas fornece informação importante sobre os mecanismos de dispersão das espécies e sua interação com fatores ambientais. Esse tipo de estudos também é de muita ajuda no desenvolvimento de planos de amostragem, na otimização de programas de manejo integrado de pragas e no planejamento de experimentos. O objetivo deste trabalho foi comparar vários modelos hierárquicos na modelagem da variação espaço-temporal da infestação da broca do café visando produzir mapas de risco da infestação que descrevam adequadamente o processo de infestação. Foram usadas diferentes combinações de efeitos aleatórios representando variabilidade não estruturada, com diferentes escolhas de distribuições a priori para os parâmetros e os hiperparâmetros dos modelos. Foram também usados diferentes esquemas de vizinhança para representar a correlação espacial dos dados. O ajuste dos modelos foi feito usando métodos MCMC. A estatística deviance e funções de perda quadrática foram usadas para a comparação entre modelos. Os resultados são apresentados como uma seqüência de mapas de risco de infestação. / Study of agricultural pests distribution in space and time provides important information about the species dispersion mechanisms and its interaction with environmental factors. It also helps the development of sampling plans, the integrated pest management and planning of experiments. The aim of this work was to compare several hierarchical models in modelling the spatio-temporal variation of the coffee berry borer infestation in order to produce risk maps. Different combinations of random effects representing spatially structured and unstructured variability were used, with different prior distributions for the parameters and hyperparameters. Also different neighbourhood schemes were used to represent the spatial correlation of the data. The model fitting was done using MCMC methods and deviance and squared loss function were used for the comparison between models. The results are presented as a sequence of risk maps.