Since plants utilize CO��� as the substrate for photosynthesis, terrestrial plants may be directly affected by increasing levels of CO��� in the atmosphere. Plants native to the sagebrush steppe are predicted to increase in growth in response to elevated CO��� through increased water use efficiency and higher photosynthetic rates. This study examined the interactions between edaphic factors and CO��� in order to determine how species native to the sagebrush steppe may respond to elevated CO���. The objectives of these experiments were to: 1. determine if Sitanion hystrix, Artemisia tridentata ssp. wyomingensis, and Stipa thurberiana alter their growth and physiology in response to CO��� and soil temperature. 2. determine if Sitanion hystrix and Artemisia tridentata ssp. wyomingensis alter their growth and physiology in response to CO��� and water stress. Two experiments were conducted using environmentally controlled chambers. In the first experiment, Sitanion hystrix, Artemisia tridentata and Stipa thurberiana were exposed to ambient (374 ppm) or high (567 ppm) CO��� conditions and low (13��C) or high (18��C) soil temperature. After four months in the chambers, plants were harvested and plant material was divided into shoots, roots, and leaves. Results from the first experiment demonstrated that carbon dioxide and soil temperature modified the growth of these species. Sitanion hystrix increased its shoot and root weights at elevated CO��� when grown under low soil temperatures. Artemisia tridentata had lower plant weights under elevated CO��� and 18 ��C soil temperature than plants grown at ambient CO��� and 13��C. Shoots of Stipa thurberiana were responsive to soil temperature and roots were responsive to CO��� at 18��C. In the second experiment, Sitanion hystrix and Artemisia tridentata were exposed to ambient (371 ppm) or high (569 ppm) CO��� and well-watered or water stressed conditions. Results indicated that there were no interactive effects betweeen CO��� and water stress with respect to plant growth or physiology. CO��� increased water use efficiency in S. hystrix and increased water use efficiency of A. tridentata at the beginning of the experiment but had no interactive effects with water stress on growth or photosynthesis. Results suggested that the effect of CO��� on plant growth and productivity of the sagebrush steppe is dependent upon the soil temperature to which the plants are exposed. Differences between species in their response to CO���, soil temperature, and water stress were also apparent in this experiment. These controlled environment studies should pave the way for field studies in the sagebrush steppe in order to determine if differences in carbon allocation, resulting from changes in CO��� and soil temperature, are realized in the field. Alterations in carbon allocation may potentially alter the competitive relationships between species and influence successional processes in the sagebrush steppe. / Graduation date: 1997

Thesis (M.S.)--Oregon State University, 2006. / Printout. Includes bibliographical references (leaves 27-31). Also available on the World Wide Web.

Thesis (M.S.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 58-61). Also available on the World Wide Web.

Herbivory and plant competition affect sexual reproduction of plants in various ways. Exclusion of mule deer (Odocoileus hemionus) and cattle, removal of plant competition (both inter- and intraspecific), and all combinations of the above treatments were used to examine the individual and combined affects on Artemisia tridentata ssp. wyomingensis (Wyoming big sagebrush) reproduction. Reproduction of Artemisia tridentata ssp. wyomingensis was divided into hierarchical levels of the number of: (1) modules per current-year ' s growth (CYG), (2) nodes per module, (3) inflorescence heads per node, (4) achenes per inflorescence head and (5) percent viable achenes. Counts at hierarchical levels were made to determine the level affected by the treatments. Deer herbivory significantly reduced reproduction at the reproductive-module-per-CYG-vegetative-biomass hierarchical level, while plant competition (both inter- and intraspecific) significantly reduced reproduction at the nodes-per-reproductive-module level and at the inflorescence-heads-per-node level. Cattle presence had neither a beneficial nor detrimental influence on reproduction during this two-year study. The combined effects of release from deer herbivory and from plant competition on reproduction was more than additive because these biotic interactions affected nested hierarchical levels.

Plant spatial patterns have been studied to gain insight into plant interactions such as competition and facilitation (positive plant interactions). The stress gradient hypothesis predicts that as environmental stress increases facilitation dominates, while competition dominates in less stressful conditions. Beneficial plants (nurses) can create favorable abiotic conditions for subanopy plants. Additionally, palatable herbaceous species growing under nurse shrub canopies benefit from physical protection. I investigated spatial associations between Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) and three native grasses (Poa secunda, Elymus elymoides, and Pseudoroegneria spicata) across a rainfall gradient in the Great Basin, USA. I also explored the effect of grazing on grass-shrub spatial associations. I hypothesized that positive shrub-grass spatial associations would become more frequent at lower rainfall levels; I further hypothesized that 1) at intermediate levels of stress, positive grass-shrub spatial associations would dominate and 2) at extreme levels of stress, positive grass-shrub spatial associations and interactions would no longer dominate. At high moisture stress, the addition of grazing stress may limit the nurse’s ability to provide to benefits to subcanopy plants. Cover of P. secunda was greater in shrub canopy microsites than interspaces at low to moderate levels of rainfall. Cover and density of E. elymoides were greater in sagebrush canopies over most rainfall levels. Elymus elymoides and P. spicata were taller and narrower in basal width and less likely to be grazed in canopy versus interspace microsites. I next investigated the effects of grazing intensity over a rainfall gradient and found a significant interaction of rainfall and microsite on P. secunda cover. Poa secunda formed positive interactions with A. tridentata at lower rainfall levels, regardless of grazing intensity. Its cover was significantly greater in interspaces at high rainfall compared to low rainfall sites. Elymus elymoides density was greater in canopy vs. interspace microsites, regardless of rainfall level or grazing intensity. Plant spatial associations can indicate which nurse microsites are favorable to plant growth and may improve seeding or planting success during ecological restoration. My results suggest that exploiting sagebrush canopy microsites for restoration of native perennial grasses would improve plant establishment, growth or survival particularly in drier areas.

Thesis (M.S.)--Oregon State University, 1994. / Typescript (photocopy). Includes bibliographical references (leaves 140-143). Also available on the World Wide Web.

Identifying genetically appropriate plant materials for seed based restoration relies on the principle of local adaptation where the objective is to match adaptive genetic characteristics to variation in ecological clines pertinent to plant establishment and persistence. In this study, basin wildrye (Leymus cinereus (Scribn. & Merr.) Á. Löve) sources from 25 wild populations and 4 commercial varieties were planted at 4 test sites. We assessed initial establishment and short term persistance. Plantings failed at 2 sites in both 2013 and 2014, with too few plants to quantify differences. At the remaining 2 sites, local sources had higher initial establishment in just 1 of 10 comparisons. Among commercial sources, the cultivars Magnar and Trailhead initially outperformed local pooled materials at Fountain Green but not at Nephi. Initial establishment under row cover was dramatically better than uncovered controls at both sites, but only persisted for 4 years after planting at the Fountain Green site. The native forb study evaluated the effects of species, sowing depth and row cover on field emergence of 20 forbs. Overall, emergence was very low ranging between 0.2% and 1.0% for 16 of the 20 species. Four species exceeded 1% emergence. Depth effects were species, site and year dependent. The odds of emergence decreased with increasing depth for four species, increased for three species and were mixed between sites and years for the remaining species. The odds of emergence were better under row cover than for uncovered control plots. Depths evaluated were deeper than recommended for most species and likely hindered emergence for some species. Site and year had much more effect on observed emergence than depth or treatment. Developing simple diagnostics to identify subspecies is key in the restoration of sagebrush ecosystems. We evaluated the SoilWeb app as a tool to identify sagebrush in the field. We evaluated the accuracy of the Richardson et.al. (2015) technique to classify sagebrush stands and evaluated data modeling strategies to improve classification accuracy. We found the SoilWeb app to be an accurate and informative tool to identify native-wild sagebrush populations. The Richardson et.al. (2015) seed weight criteria correctly classified just 19% of our sample populations to the correct subspecies. To improve upon this, we evaluated multifactor modeling using recursive partitioning and classification trees. Our most accurate classification tree correctly classified 80% of 2x tridentata sites but just 45% of wyomingensis sites.

Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) are a species of conservation concern in the rangelands of western North America due to their dramatic decline over the last half century. Effective conservation and management of sensitive species requires an understanding of how species respond to management actions. We examined two aspects of the reproductive phases of sage-grouse: nest predation, and habitat selection by female sage-grouse with chicks. In Chapter 1, we developed resource selection functions to assess the influence of mechanical treatments of mountain big sagebrush (Artemisia tridentata vaseyana) on habitat selection by greater sage-grouse with chicks. Post-treatment sage-grouse showed stronger selection for treatments and treatment edges than did pre-treatment sage-grouse. This altered pattern of selection by sage-grouse with broods suggests mechanical treatments may be a suitable way to increase use of mountain big sagebrush during the brooding period. In Chapter 2, we assessed the effect of habitat edges on nest predation of sage-grouse. The "edge effect" hypothesis states that habitat edges are associated with reduced nest success for birds. We tested the edge effect hypothesis using 155 nest locations from 114 sage-grouse. We derived edge metrics for 11 habitat cover types to determine which variables may have affected nest predation. We found support for the edge effect hypothesis in that nest predation increased with increasing edge density of paved roads. We provide evidence that the edge effect hypothesis may apply to greater sage-grouse and their habitats. Based on our results, we recommend minimizing disturbances that fragment critical nesting habitat of greater sage-grouse.

Crested wheatgrass (Agropyron cristatum [L.] Gaertn.) is one of the most commonly seeded grass species in the western United States and dominates thousands of hectares in the Great Basin. Although many degraded Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) plant communities have been seeded with crested wheatgrass, successional pathways, influence of soil attributes, and cultivation history on the vegetation of these communities have not been fully characterized. I sought to identify community phases, vegetative differences, and soil attributes that explain variation among 35 Wyoming big sagebrush communities historically seeded with crested wheatgrass. All communities were more than 30 years old and had not experienced fire, or received subsequent chemical or mechanical treatments following their original seeding. Species richness, diversity, vegetation cover, and soil samples were measured in four 20 x 5 m intensive Modified Whittaker plots per community. Hierarchical clustering and principal component analysis of three indicator species (crested wheatgrass, Sandberg bluegrass, and Wyoming big sagebrush) identified four distinct community phases. Community phase 1 was dominated by crested wheatgrass and had the lowest species richness and cover of big sagebrush. Phases 2 and 3 had the highest species richness and cover of native species. Phase 4 was dominated by big sagebrush and had the lowest cover of crested wheatgrass. Community phases differed significantly for soil texture, soil nitrogen, and ground cover characteristics. Bare soil was almost double on loam-textured soils and rock cover was higher on clay loam texture soils (P < 0.05) as well as native plant cover. Communities previously cropped occurred on more coarse-textured soils and had 6-fold lower native species cover and double exotic herbaceous and crested wheatgrass cover. Cropping occurred on favorable, low rock, fine-texture soils, the same soils that favor crested wheatgrass production and reduce resilience of native plant composition. Delineation of community phases provided a new, empirically based state-and-transition model, while the characterization of soil attributes and disturbance history provided information about feedback mechanisms influencing dominant species that delineate community phases and effect community structure. This information can be used to assist in the development of management strategies in crested wheatgrass seeded communities.

This study consisted of two research projects in the Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis (Beetle & A. Young) S.L. Welsh) alliance, the most extensive of the big sagebrush complex in the Intermountain West. In the first project, we intensively sampled 107 relatively undisturbed, late seral Wyoming big sagebrush sites across the High Desert, Humboldt, and western Snake River Ecological Provinces to investigate vegetation heterogeneity and the relationship of environmental factors with vegetation characteristics. Vegetation characteristics were highly variable across the region. Perennial grass and total herbaceous cover varied more than six and sevenfold, respectively between minimum and maximum values. Sagebrush cover averaged 12%, but ranged between 3 and 25%. With the exception of perennial grass cover (p<0.0001, r²=0.52), limited variability in other vegetation characteristics was explained by environmental variables. In the second project, we investigated the functional role of Wyoming big sagebrush by using undisturbed and sagebrush removed (with burning) treatments and comparing vegetation and microsite characteristics under (subcanopy) to between sagebrush canopy (interspace) zones. Wyoming big sagebrush influenced associated vegetation and microsites. On sites receiving high incidental radiation, perennial grass and total herbaceous cover and density were greater in the subcanopy than interspace zones (p<0.05). On north aspects, these differences were not as pronounced suggesting sagebrush's influence on associated vegetation is site dependent. Temperature extremes were mediated and soil water content was greater in the subcanopy than interspace zones during the growing season. Results indicated that the subcanopy zone can be a more favorable environment to herbaceous vegetation than the interspace zone. Wyoming big sagebrush is important to community resource capture and use. Plots with sagebrush had greater soil water content at the start of the growing season and produced more total biomass compared to where sagebrush had been removed in both post-fire years (p<0.05). However, higher Thurber's needlegrass photosynthetic rates and greater herbaceous cover and production where sagebrush had been removed suggested that more resources were available to herbaceous vegetation in the absence of sagebrush. / Graduation date: 2006