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Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-04-23T18:17:21Z No. of bitstreams: 1 Dissertacao kercya-sec1.pdf: 1781506 bytes, checksum: c9c18265f1725a7550157208c0eeb542 (MD5) / Approved for entry into archive by Rodrigo Meirelles(rodrigomei@ufba.br) on 2013-05-08T11:43:37Z (GMT) No. of bitstreams: 1 Dissertacao kercya-sec1.pdf: 1781506 bytes, checksum: c9c18265f1725a7550157208c0eeb542 (MD5) / Made available in DSpace on 2013-05-08T11:43:37Z (GMT). No. of bitstreams: 1 Dissertacao kercya-sec1.pdf: 1781506 bytes, checksum: c9c18265f1725a7550157208c0eeb542 (MD5) Previous issue date: 2004 / Devido à semelhança entre a composição química do Agregado de Trióxido Mineral (MTA) e o cimento Portland, várias pesquisas vêm sendo realizadas a fim de viabilizar o uso odontológico do cimento Portland. O objetivo do presente estudo foi analisar o comportamento biológico do tecido conjuntivo submucoso de ratos, ao implante de tubos de polietileno contendo MTA, cimento Portland, cimento Portland acrescido de 20% de óxido de bismuto, nos tempos experimentais de 1, 2 e 12 semanas. Foram utilizados 45 ratos, distribuídos aleatoriamente em três grupos de quinze animais, de acordo com os tempos experimentais. Em cada animal foram realizados dois leitos cirúrgicos na região de filtro superior, onde do lado direito se colocava o grupo experimental e do lado esquerdo, o grupo controle. Decorridos os períodos experimentais, cinco animais de cada grupo foram sacrificados e as características do processo inflamação-reparo foram avaliadas pela técnica histológica de rotina e pela coloração de picrosirius red, específica para colágeno. A análise estatística dos resultados mostrou que não havia diferença estatisticamente significante entre os diferentes materiais testados nos três tempos analisados. Em todos os grupos observou-se decréscimo da resposta inflamatória nos tempos mais prolongados e aumento da quantidade e organização das fibras colágenas denotando que todos os materiais avaliados, implantados em tecido submucoso de ratos, proporcionam reparo a longo prazo. / Salvador

Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2014-11-18T14:44:30Z No. of bitstreams: 2 Dissertacao - Jean Carlos Barbosa Ferreira - 2014.pdf: 2525407 bytes, checksum: 706cd059143385efad393d4ecd42839c (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-11-18T15:42:30Z (GMT) No. of bitstreams: 2 Dissertacao - Jean Carlos Barbosa Ferreira - 2014.pdf: 2525407 bytes, checksum: 706cd059143385efad393d4ecd42839c (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-11-18T15:42:30Z (GMT). No. of bitstreams: 2 Dissertacao - Jean Carlos Barbosa Ferreira - 2014.pdf: 2525407 bytes, checksum: 706cd059143385efad393d4ecd42839c (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-02-14 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Pleomorphic adenoma (PA) is the most common salivary gland tumor, however its etiopathogenesis is unclear, as well as your neoplastic potential. Studies have already been done investigating apoptosis, mucosecretory activity and proliferation cellular, although these studies are controversial. The aim of this study was to investigate the neoplastic potential of the PA of minor oral salivary glands measured by apoptosis (Bcl-2, Bax and p53), mucosecretory activity (MUC1), and cellular proliferation (Ki-67). Thirty-one cases of PA of the oral cavity and 4 controls (C) taken from normal oral minor salivary glands were analyzed by immunohistochemistry technique. The proteins were detected utilizing a semi-quantitative method (scores) as follows: (-) negative ≤ 5%, (+) low 6–25%, (++) moderate 26–50% and (+++) high >50% of positive tumour cells. The apoptotic indices were evaluated by the ratio Bcl-2/Bax. Non-parametric comparison and correlation tests were used for analysis. The data showed high staining of anti-apoptotic protein Bcl-2 in both groups (PA= 57.9%; C=67.7%) and an expression significantly lower of pro-apoptotic protein Bax (PA=22.7%; C=97.7%) and MUC1 (PA=14%; C=82.3%) in PA than in C (p<0.001). On the other hand, we observed a similar expression of Ki-67 and p53 proteins (≤ 5%) in both PA and C. In PA, only 2 cases showed the ratio Bcl-2/Bax <1. There was no difference in cellular expression with regard to clinical variables clinical and outcome (p>0.05). The neoplastic potential of PA can be associated with an imbalance in apoptotic processes and a lower index of proliferation cellular and that the mucosecretory activity does not play a significant role in primary PA. / Adenoma Pleomórfico (AP) é o tumor de glândula salivar mais comum, entretanto sua etiopatogênese permanece incerta bem como seu potencial neoplásico. Estudos têm sido realizados no intuito de investigar apoptose, atividade mucosecretora e proliferação celular em AP, entretanto estes trabalhos são controversos. O objetivo deste estudo foi investigar o potencial neoplásico do AP de glândulas salivares menores de boca mensurados pela apoptose (Bcl-2, Bax e p53), atividade mucosecretora (MUC1) e proliferação celular (Ki-67). Trinta e um casos de AP da cavidade oral e quatro controles (C), biopsiadas de glândulas salivares menores orais normais foram analisadas através da técnica imuno-histoquímica. As proteínas foram detectadas utilizando um método semi-quantitativo (scores) seguindo os seguintes critérios: (-) negativa ≤ 5%, (+) baixa 6-25%, (+ +) moderada 26-50% e (+ + +) alta > 50% expressão de células tumorais marcadas positivamente. Para avaliação dos índices de apoptose, a razão Bcl-2/Bax foi utilizada. Comparação não paramétrica e testes de correlação foram utilizados para a análise. Os dados mostraram elevada marcação de proteína antiapoptótica Bcl-2 em ambos os grupos (AP = 57,9% C = 67,7%) e uma expressão significativamente mais baixa da proteína pró-apoptótica Bax (AP = 22,7% C = 97,7%) e MUC1 (AP = 14% C = 82,3%) em AP do que em C (p <0,001). Por outro lado, observou-se uma expressão semelhante de Ki-67 e p53 (≤ 5%) em ambos os grupos. Em AP, apenas 2 casos apresentaram a relação Bcl-2/Bax <1. Não houve diferença na expressão celular, no que diz respeito às variáveis clínicas e o desfecho (p> 0,05). O potencial neoplásico do AP pode ser associado a um desequilíbrio nos processos apoptóticos, e os baixos índices de proliferação celular e a atividade mucosecretora parece não desempenhar papel significativo no AP primários.

Accélérer la dégradation anaérobie des déchets enfouis, optimiser la production de biogaz et diminuer le temps et le coût de surveillance sont les enjeux principaux d'installation de stockage des déchets non dangereux (ISDND)-bioactives, ainsi que, plus classiquement, minimiser leurs impacts sanitaires et environnementaux. L'une des méthodes les plus efficaces pour atteindre ces objectifs est la recirculation de lixiviat et l'augmentation de l'humidité des déchets. Les objectifs du bioréacteur ne seront pas atteints sans une connaissance rationnelle des phénomènes hydrauliques, biologiques et thermiques qui s’y développent et de l’influence de l'un de ces phénomènes sur les autres. Les observations in situ, les expérimentations en laboratoire ainsi que les modèles numériques permettent ensemble une approche rationnelle de ces phénomènes. C’est ce qui constitue le corps de ce travail de thèse, où nous avons étudié le comportement hydro-thermo-biologique des déchets dans la phase anaérobie en laboratoire, sur site à partir de données hydro-thermiques de deux bioréacteurs situés en France et en développant un modèle numérique pour simuler ce comportement couplé des bioréacteurs. Les travaux en laboratoire nous ont permis d’étudier l’effet de la saturation et de la densité (compactage des déchets) sur la dégradation anaérobie des déchets ménagers et l’influence de ces paramètres sur la production de biogaz. Les données hydrauliques et thermiques in-situ des bioréacteurs nous ont permis de connaître les variations des paramètres essentiels comme la température et la saturation dans les déchets, à différentes profondeurs, et estimer d’autres paramètres qui sont difficile à déterminer expérimentalement. Le modèle numérique nous a permis d’étudier le comportement couplé, hydro-thermo-biologique, des bioréacteurs à long terme (pendant une dizaine d’années) aussi bien qu’à court terme pendant la recirculation de lixiviat. L’interdépendance des différents paramètres qui influent la dégradation des déchets est la principale raison nous ayant conduits à développer un modèle de couplage qui nous permette d'étudier chaque paramètre en fonction des autres. Les travaux en laboratoire et les données thermiques de site nous ont conduits à développer un modèle d'écoulement diphasique du liquide et du gaz dans les déchets, considérant les phénomènes biologiques, en fonction des paramètres clés de la dégradation comme la température et la saturation, pour aboutir à la production de biogaz et de chaleur. Les trois parties de ce travail, les expérimentations en laboratoire, le développement d'un modèle numérique et l’analyse des données de site ont été effectuées en parallèle de façon complémentaire. Les expérimentation de laboratoire tout comme l’analyse des données de site, nous ont montré l'importance des paramètres qu'il faut considérer dans le modèle et en retour le modèle numérique nous a aidé à diriger les expérimentations en laboratoire et montré la nécessité de conduire certaines analyses sur les pilotes expérimentaux, comme l’analyse de la biomasse, de la DCO et des AGV. L'analyse des données hydrauliques et thermiques de sites de bioréacteur nous a permis de caler les paramètres hydrauliques, biologiques et thermiques des déchets qui sont difficile à définir sur le site sans le perturber (comme la conductivité hydraulique, la saturation, la conductivité thermique, la capacité calorifique, la concentration en biomasse et en AGV). Le travail réalisé dans la thèse a permis de développer un modèle couplé hydro-thermo-biologique et de tester sa capacité à prévoir le comportement thermique d'un bioréacteur, la production totale et le taux de production de méthane. Nous avons montré qu'il était adopté à l'étude du comportement à long terme d'un bioréacteur, aussi bien qu'à court terme pendant la réinjection de lixiviat, là où les techniques de mesure et le temps sont limitants en laboratoire ou sur site / The main objectives of bioreactor landfills are to accelerate anaerobic degradation of waste in order to minimize the environmental impacts, to optimize biogas production and to minimize the time of waste stabilization as well as the costs and time of monitoring of landfill sites after operation. One of the most important and cost-effective method to achieve these objectives is liquid addition and management. The objectives of bioreactor landfills could not be achieved without enough knowledge of its hydraulic, thermal and biological parameters and processes and the effects of each of them on the others. Site observations and data and laboratory experiments as well as numerical models could help to develop the knowledge of these phenomena and processes, which is the objective of this work. In this thesis we study the coupled hydro-thermo-biological behavior of bioreactor landfills in the anaerobic phase in the laboratory and using site data of two bioreactor landfills in France and developing a numerical coupled model. The laboratory experiments help us to know the effect of such important parameters as saturation and density of wastes on anaerobic degradation and biogas production. The site data help us to know the variations of saturation and temperature of wastes in a bioreactor landfill in different depths, as two key factors of anaerobic degradation and biogas production. Site analysis helps also to estimate some parameters as hydraulic and thermal conductivity of wastes, which are hard to measure in situ without disturbing the landfill site. The numerical model helps us to study the coupled behavior of bioreactor landfills during leachate recirculation, as well as on the long term during many years. The interdependence of various parameters which influence waste degradation and thermo-biological phenomena in a bioreactor landfills is the main reason of development of this coupled model. This model makes it possible to study each key parameter, as saturation and temperature, as a function of other parameters. Laboratory experiments and site data analysis lead to develop a biological model of degradation to be coupled with a two-phase flow model of liquid and gas. The three parts of this thesis, laboratory experiments, site data analysis and development of the numerical coupled model were carried out in parallel and in a complementary manner. Laboratory experiments as well as site data analysis showed us the importance of some parameters to be considered in the numerical model and coupled behavior. In return numerical model showed the importance of considering the temperature dependence behavior of microbial activity and the necessity of biomass, VFA and COD analysis in laboratory experiments. The analysis of hydraulic and thermal site data led to estimate parameters which are hard to measure in situ or in the laboratory, as hydraulic and thermal conductivity of waste, saturation, thermal conductivity of cover layer and heat capacity of waste. The numerical coupled hydro-thermo-biological model seems to be efficient enough to predict biogas and methane production in bioreactor and classical landfills and to reproduce their correct behavior