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In vitro and in vivo characterization of a cell source for bone tissue engineering applications primary bone marrow stromal cells overexpressing the osteoblast-specific transcriptional activator Runx2/Cbfa1 /Byers, Benjamin Allen. January 2003 (has links) (PDF)
Thesis (Ph. D.)--Mechanical Engineering, Georgia Institute of Technology, 2004. / Joseph M. LeDoux, Committee Member ; Julia E. Babensee, Committee Member ; Robert E. Guldberg, Committee Member ; Andres J. Garcia, Committee Chair ; Grace K. Pavlath, Committee Member ; Barbara D. Boyan, Committee Member. Includes bibliographical references.
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Four differentially expressed cDNAs containing the Rebers-Riddiford consensus sequence in Callinectes sapidus /Wynn, Anna. January 2004 (has links) (PDF)
Thesis (M.S.)--University of North Carolina at Wilmington, 2004. / Includes bibliographical references (leaves : [38]-[40]).
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Differential effects of arachidonic acid and docosahexaenoic acid on cell biology and osteoprotegerin synthesis in osteoblast-like cellsCoetzee, Magdalena. January 2005 (has links)
Thesis (PhD.(Physiology)--Faculty of Health Sciences)-University of Pretoria, 2005. / Summary in English and Afrikaans. Also available on the Internet via the World Wide Web.
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Differential expression of eight transcripts and their roles in the cuticle of the blue crab, Callinectes sapidus /Faircloth, Lindsay January 2006 (has links) (PDF)
Thesis (M.S.)--University of North Carolina at Wilmington, 2006. / Includes bibliographical references (leaves: 40-42)
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Biodeposição de CaCO3 em materiais cimentícios : contribuição ao estudo da biomineralização induzida por Bacillus subtilisVieira, Juliana Aparecida January 2017 (has links)
A indústria da construção civil é conhecida como umas das atividades econômicas que causam os maiores impactos ambientais desde o processo de extração da matéria prima até a produção dos produtos, incluindo o transporte e manutenção do ambiente construído. A produção de um dos seus principais componentes, o cimento, é o maior contribuinte para a emissão de gases de efeito estufa, principalmente devido a queima de combustíveis fósseis. Por este motivo, pesquisas na área de biotecnologia sustentável são conduzidas para diminuir e até mitigar os efeitos danosos provocados pelos fatores que compõem a construção civil. Dentre estas pesquisas destacam-se as que se baseiam na Biomimética, que é uma ciência que busca na Natureza as soluções tecnológicas para os problemas que os desenvolvimentos humanos geralmente apresentam: a geração de resíduos poluentes, uso de produtos químicos tóxicos e processos que operam com energia e pressão elevadas. Com base nos conceitos biomiméticos, este trabalho se propôs a estudar a biomineralização, que é um processo que ocorre na Natureza a milhares de anos e é responsável pela formação de muitas estruturas biomineralizadas tanto no ambiente terrestre como aquático. A biomineralização é um fenômeno provocado pela ação de diversas espécies de microrganismos que durante o processo de obtenção de energia reciclam minerais presentes no solo e na água e os precipitam na forma de sais inorgânicos. Este material precipitado age como agente ligante de partículas como no caso de formações geológicas (estromatólitos) ou exoesqueletos de animais marinhos, por exemplo. Neste estudo foi avaliado a biomineralização por biodeposição de carbonato de cálcio precipitado na presença da espécie de bactéria ureolítica (Bacillus subtilis) em ensaios em escala laboratorial utilizando corpos de prova de areia, argamassa e concreto. Os corpos de prova em areia e argamassa foram observados em MEV e EDS permitindo a identificação de células de microrganismos, formação de biofilme e provável formação de cristais de carbonato de cálcio na região de biofilme. Os corpos de prova de concreto foram utilizados para avaliar as consequências da biodeposição na absorção de água por capilaridade do material. Resultados indicam redução de 20% na absorção de água por capilaridade. Com os resultados obtidos é possível concluir que a técnica de biodeposição pode ser uma alternativa ao tratamento superficial de estruturas de concreto, contudo requer estudos posteriores de aplicação técnica e viabilidade econômica. / The construction industry has been known as one of the economic activities that cause the major environment impacts since the process of raw material extraction until the products manufacturing including transport and maintenance of the built environment. The production of one of the main compounds, the cement, is the largest contributor to the greenhouse gas emissions, mainly due to burn fossil fuels. For this reason, researches in sustainable biotechnological area are conducted to minimize and even mitigate the damaging effects either promoted by construction industry factors. Among these ones, it stands out researches based on Biomimetic, which is a science that seeks in Nature the technological solutions for problems that human’s development usually presents: the generation of pollutant residues, the use of toxic chemicals and process that operates in high pressure and energy. Based on biomimetic concepts this research proposes to study the biomineralization, which is a process that has occurred in the Nature for thousands of years and it is responsible for the formation of many structures either in soil and water environments. The biomineralization is a phenomenon caused by several specimens of microorganisms that during the process of obtaining energy, they recycle minerals presents at soil and water inducing precipitation as inorganic salts. This precipitated material works as a binder of particles similar to geologic formations (stromatolites) or exoskeleton of sea animal for example. In this study the biomineralization was evaluated through biodeposition of precipitated calcium carbonate by specimen of ureolytic bacteria (Bacillus subtilis). Essays were held using samples made by sand, mortar and concrete. The samples made by sand and mortar were observed at MEV and EDS, allowing the identification of microorganism cells, biofilm formation and probable formation of calcium carbonate crystals at biofilm region. The concrete samples were used to evaluate the consequences of biodeposition on water absorption by capillarity of the material. The results show reduction of 20% on water absorption by capillarity. According the results achieved it possible to conclude that the biodeposition technique can be an alternative to superficial treatment for concrete structures. However, it will be required more studies to evaluate technical application and economical availability.
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Biological synthesis of metallic nanoparticles and their interactions with various biomedical targetsSennuga, Afolake Temitope January 2012 (has links)
The synthesis of nanostructured materials, especially metallic nanoparticles, has accrued utmost interest over the past decade owing to their unique properties that make them applicable in different fields of science and technology. The limitation to the use of these nanoparticles is the paucity of an effective method of synthesis that will produce homogeneous size and shape nanoparticles as well as particles with limited or no toxicity to the human health and the environment. The biological method of nanoparticle synthesis is a relatively simple, cheap and environmentally friendly method than the conventional chemical method of synthesis and thus gains an upper hand. The biomineralization of nanoparticles in protein cages is one of such biological approaches used in the generation of nanoparticles. This method of synthesis apart from being a safer method in the production of nanoparticles is also able to control particle morphology. In this study, a comparative biological synthesis, characterization and biomedical effects of metallic nanoparticles of platinum, gold and silver were investigated. Metallic nanoparticles were biologically synthesized using cage-like (apoferritin), barrel-like (GroEL) and non-caged (ribonuclease) proteins. Nanoparticles generated were characterized using common techniques such as UV-visible spectroscopy, scanning and transmission electron microscopy, inductively coupled optical emission spectroscopy, Fourier transform infra-red spectroscopy and energy dispersion analysis of X-rays (EDAX). Nanoparticles synthesised biologically using apoferritin, GroEL and RNase with exhibited similar chemical and physical properties as thoses nanoparticles generated chemically. In addition, the metallic nanoparticles fabricated within the cage-like and barrel-like cavities of apoferritin and GroEL respectively, resulted in nanoparticles with relatively uniform morphology as opposed to those obtained with the non-caged ribonuclease. The enzymatic (ferroxidase) activity of apoferritin was found to be greatly enhanced with platinum (9-fold), gold (7-fold) and silver (54-fold) nanoparticles. The ATPase activity of GroEL was inhibited by silver nanoparticles (64%), was moderately activated by gold nanoparticles (47%) and considerably enhanced by platinum nanoparticles (85%). The hydrolytic activity of RNase was however, lowered by these metallic nanoparticles (90% in Ag nanoparticles) and to a higher degree with platinum (95%) and gold nanoparticles (~100%). The effect of synthesized nanoparticles on the respective enzyme activities of these proteins was also investigated and the potential neurotoxic property of these particles was also determined by an in vitro interaction with acetylcholinesterase. Protein encapsulated nanoparticles with apoferrtin and GroEL showed a decreased inhibition of acetylcholinesterase (<50%) compared with nanoparticles attached to ribonuclease (>50%). Thus, it can be concluded that the cavities of apoferitin and GroEL acted as nanobiofactories for the synthesis and confinement of the size and shape of nanoparticles. Furthermore, the interior of these proteins provided a shielding effect for these nanoparticles and thus reduced/prevented their possible neurotoxic effect and confirmed safety in their method of production and application. The findings from this study would prove beneficial in the application of these nanoparticles as a potential drug/drug delivery vehicle for the prevention, treatment/management of diseases associated with these enzymes/proteins.
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Fósforo disponível na mineralização óssea de alevinos da Tilápia do NiloRocha, Mariucha Karina Honório Ribeiro [UNESP] 01 June 2012 (has links) (PDF)
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rocha_mkhr_me_botfmvz.pdf: 206056 bytes, checksum: b4220d7b33a179c4a5950580e30d0d86 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Universidade Estadual Paulista (UNESP) / Os objetivos desta pesquisa foram avaliar a quantidade de fósforo disponível presente em rações experimentais e por meio delas determinar a exigência do mineral para adequada mineralização óssea de alevinos da tilápia do Nilo. Foram formuladas quatro dietas para conter níveis de 2,0; 4,0 e 6,0g kg-1 de fósforo disponível utilizando-se fosfato bicálcico e uma dieta sem suplementação (1,5g kg-1). Todas acrescidas de 1,0g kg-1 de Cr2O3. Para a determinação do coeficiente de disponibilidade do fósforo foram distribuídos 160 peixes (peso médio de 30g + 0,5) em oito aquários, alimentados com as dietas experimentais para coleta de fezes. A exigência de fósforo foi avaliada utilizando grupos de peixes com peso inicial médio de 1,2g + 0,5; sendo alimentados quatro vezes ao dia durante 90 dias até a saciedade aparente. Foram avaliados sobrevivência, ganho de peso, porcentagem de cálcio e fósforo presentes nas vértebras, concentração de fósforo presente na água, densitometria óssea (vértebras e corpo) e imagens tomográficas. As melhores respostas apresentadas pelos peixes foram entre 4,0 e 6,0g kg-1 de fósforo disponível nas dietas / The aim of this study were to evaluate the amount of present available phosphorus in the experimental diets and through them to determine minimum requirement of the mineral for proper bone mineralization of Nile tilapia fingerlings. Four diets were formulated to contain levels of 2.0, 4.0 and 6.0 g kg-1 available phosphorus by calcium phosphate and a diet without supplementation (1.5 g kg-1). All diets were added 1,0g kg-1 Cr2O3. To determine the coefficient of phosphorus availability were distributed 160 fish (average weight 30g + 0.5) in eight tanks, fed the experimental diets for feces. The requirement of phosphorus was evaluated in fish with initial average weight of 1.2g+ 0.5, being fed four times daily for 90 days until satiation. Were evaluated survival, weight gain, percentage of calcium and phosphorus in the vertebrae, phosphorus concentration in the water, bone densitometry (vertebrae and body) and tomography images. The best responses were submitted by the fish between 4.0 and 6.0 g kg-1 of available phosphorus in the diets
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Molecular characterisation of biomineralising genes in the freshwater pond snail Lymnaea stagnalisHerlitze, Ines 14 September 2017 (has links)
No description available.
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Fósforo disponível na mineralização óssea de alevinos da Tilápia do Nilo /Rocha, Mariucha Karina Honório Ribeiro, 1983. January 2012 (has links)
Orientador: Luiz Edivaldo Pezzato / Coorientador: Edma Miranda de Carvalho / Banca: Hamilton Hisano / Banca: Vânia Vasconcelos / Resumo: Os objetivos desta pesquisa foram avaliar a quantidade de fósforo disponível presente em rações experimentais e por meio delas determinar a exigência do mineral para adequada mineralização óssea de alevinos da tilápia do Nilo. Foram formuladas quatro dietas para conter níveis de 2,0; 4,0 e 6,0g kg-1 de fósforo disponível utilizando-se fosfato bicálcico e uma dieta sem suplementação (1,5g kg-1). Todas acrescidas de 1,0g kg-1 de Cr2O3. Para a determinação do coeficiente de disponibilidade do fósforo foram distribuídos 160 peixes (peso médio de 30g + 0,5) em oito aquários, alimentados com as dietas experimentais para coleta de fezes. A exigência de fósforo foi avaliada utilizando grupos de peixes com peso inicial médio de 1,2g + 0,5; sendo alimentados quatro vezes ao dia durante 90 dias até a saciedade aparente. Foram avaliados sobrevivência, ganho de peso, porcentagem de cálcio e fósforo presentes nas vértebras, concentração de fósforo presente na água, densitometria óssea (vértebras e corpo) e imagens tomográficas. As melhores respostas apresentadas pelos peixes foram entre 4,0 e 6,0g kg-1 de fósforo disponível nas dietas / Abstract: The aim of this study were to evaluate the amount of present available phosphorus in the experimental diets and through them to determine minimum requirement of the mineral for proper bone mineralization of Nile tilapia fingerlings. Four diets were formulated to contain levels of 2.0, 4.0 and 6.0 g kg-1 available phosphorus by calcium phosphate and a diet without supplementation (1.5 g kg-1). All diets were added 1,0g kg-1 Cr2O3. To determine the coefficient of phosphorus availability were distributed 160 fish (average weight 30g + 0.5) in eight tanks, fed the experimental diets for feces. The requirement of phosphorus was evaluated in fish with initial average weight of 1.2g+ 0.5, being fed four times daily for 90 days until satiation. Were evaluated survival, weight gain, percentage of calcium and phosphorus in the vertebrae, phosphorus concentration in the water, bone densitometry (vertebrae and body) and tomography images. The best responses were submitted by the fish between 4.0 and 6.0 g kg-1 of available phosphorus in the diets / Mestre
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Biodeposição de CaCO3 em materiais cimentícios : contribuição ao estudo da biomineralização induzida por Bacillus subtilisVieira, Juliana Aparecida January 2017 (has links)
A indústria da construção civil é conhecida como umas das atividades econômicas que causam os maiores impactos ambientais desde o processo de extração da matéria prima até a produção dos produtos, incluindo o transporte e manutenção do ambiente construído. A produção de um dos seus principais componentes, o cimento, é o maior contribuinte para a emissão de gases de efeito estufa, principalmente devido a queima de combustíveis fósseis. Por este motivo, pesquisas na área de biotecnologia sustentável são conduzidas para diminuir e até mitigar os efeitos danosos provocados pelos fatores que compõem a construção civil. Dentre estas pesquisas destacam-se as que se baseiam na Biomimética, que é uma ciência que busca na Natureza as soluções tecnológicas para os problemas que os desenvolvimentos humanos geralmente apresentam: a geração de resíduos poluentes, uso de produtos químicos tóxicos e processos que operam com energia e pressão elevadas. Com base nos conceitos biomiméticos, este trabalho se propôs a estudar a biomineralização, que é um processo que ocorre na Natureza a milhares de anos e é responsável pela formação de muitas estruturas biomineralizadas tanto no ambiente terrestre como aquático. A biomineralização é um fenômeno provocado pela ação de diversas espécies de microrganismos que durante o processo de obtenção de energia reciclam minerais presentes no solo e na água e os precipitam na forma de sais inorgânicos. Este material precipitado age como agente ligante de partículas como no caso de formações geológicas (estromatólitos) ou exoesqueletos de animais marinhos, por exemplo. Neste estudo foi avaliado a biomineralização por biodeposição de carbonato de cálcio precipitado na presença da espécie de bactéria ureolítica (Bacillus subtilis) em ensaios em escala laboratorial utilizando corpos de prova de areia, argamassa e concreto. Os corpos de prova em areia e argamassa foram observados em MEV e EDS permitindo a identificação de células de microrganismos, formação de biofilme e provável formação de cristais de carbonato de cálcio na região de biofilme. Os corpos de prova de concreto foram utilizados para avaliar as consequências da biodeposição na absorção de água por capilaridade do material. Resultados indicam redução de 20% na absorção de água por capilaridade. Com os resultados obtidos é possível concluir que a técnica de biodeposição pode ser uma alternativa ao tratamento superficial de estruturas de concreto, contudo requer estudos posteriores de aplicação técnica e viabilidade econômica. / The construction industry has been known as one of the economic activities that cause the major environment impacts since the process of raw material extraction until the products manufacturing including transport and maintenance of the built environment. The production of one of the main compounds, the cement, is the largest contributor to the greenhouse gas emissions, mainly due to burn fossil fuels. For this reason, researches in sustainable biotechnological area are conducted to minimize and even mitigate the damaging effects either promoted by construction industry factors. Among these ones, it stands out researches based on Biomimetic, which is a science that seeks in Nature the technological solutions for problems that human’s development usually presents: the generation of pollutant residues, the use of toxic chemicals and process that operates in high pressure and energy. Based on biomimetic concepts this research proposes to study the biomineralization, which is a process that has occurred in the Nature for thousands of years and it is responsible for the formation of many structures either in soil and water environments. The biomineralization is a phenomenon caused by several specimens of microorganisms that during the process of obtaining energy, they recycle minerals presents at soil and water inducing precipitation as inorganic salts. This precipitated material works as a binder of particles similar to geologic formations (stromatolites) or exoskeleton of sea animal for example. In this study the biomineralization was evaluated through biodeposition of precipitated calcium carbonate by specimen of ureolytic bacteria (Bacillus subtilis). Essays were held using samples made by sand, mortar and concrete. The samples made by sand and mortar were observed at MEV and EDS, allowing the identification of microorganism cells, biofilm formation and probable formation of calcium carbonate crystals at biofilm region. The concrete samples were used to evaluate the consequences of biodeposition on water absorption by capillarity of the material. The results show reduction of 20% on water absorption by capillarity. According the results achieved it possible to conclude that the biodeposition technique can be an alternative to superficial treatment for concrete structures. However, it will be required more studies to evaluate technical application and economical availability.
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