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Depot differences in adipose tissue metabolism and function in obese black South African women and changes in response to an exercise training interventionNono Nankam, Pamela Arielle 18 February 2021 (has links)
Black South African (SA) women are disproportionally affected by obesity and insulin resistance, which have been associated with depot-specific alterations in adipose tissue function. This thesis aimed to evaluate the differences in fatty acid (FA) composition and gene expression between abdominal (aSAT) and gluteal subcutaneous adipose tissue (gSAT), and the changes in response to exercise training in relation to body composition, hepatic fat, inflammatory and oxidative stress markers, and insulin sensitivity (SI) in obese black SA women. This research evaluated the i) FA composition of aSAT and gSAT, and red blood cell total phospholipids (RBC-TPL) and their associations with body composition, hepatic fat and SI, ii) changes in these FA profiles in response to exercise training and the relationship with changes in systemic inflammation, hepatic fat and SI; iii) effects of exercise training on systemic markers and SAT gene expression of inflammation and oxidative stress; and iv) regional differences in transcriptome profiles of aSAT and gSAT pre- and post-exercise training. Forty-five IsiXhosa women (30-40kg/m2 , 20-35 years) were randomized into control (n=22) or exercise groups (n=23; 12-week aerobic-resistance training, 40-60 min/session, 4 days/week). Pre and postintervention measurements included: anthropometry, body composition, cardiorespiratory fitness, dietary intake, SI, hepatic fat, systemic markers and SAT gene expression of adipokines, inflammation and oxidative stress, RBC-TPL and SAT fatty acids profiles, and untargeted SAT gene expression analyses. The main findings showed differences in the circulating (RBC-TPL) and stored (SAT) FA composition, which reflected in different associations between these FA profiles and SI. Moreover, the changes in FA composition in response to exercise training were depot-specific, with the changes in RBC-TPL correlating with a decrease in systemic inflammation and hepatic fat. Exercise training alleviated systemic oxidative stress and induced increased gSAT inflammatory genes, reflecting SAT remodelling. These changes coincided with a reduction in gynoid fat and were not associated with increased SI. Furthermore, there were unique depot-specific gene expression signatures relating to embryonic development at baseline and more diverse functional-related processes at post-training. This generated novel candidate genes potentially implicated in the relationship between body fat distribution and metabolic status in obese black SA women.
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Money and power in household management: experiences of Black South African womenGcabo, R.P.E. (Rebone Prella Ethel) 29 January 2004 (has links)
The aim of this thesis was to explore the experiences of black, married, working, South African women in relation to financial decision-making processes within private households from a working-woman’s perspective. The focus was on married women in middle and senior management positions in their workplaces. Following a literature review to accumulate empirical evidence from similar studies in the areas of Economics, Sociology, Psychology, Feminism and Economic Psychology, eight, individual, semi-structured interviews were conducted with black South African women in managerial positions to establish the women’s understanding of the meaning of money, concepts and practices of sharing of monetary resources between husband and wife in the household, the allocation of money as a resource in the household, control of money between husband and wife in the household, and decision-making processes between husband and wives. The key findings of the study were: · The diverse construction of the meaning of money. Women’s views on money had an impact on how they viewed their roles in household financial management and decision-making. · The absence of equal sharing of money and the existence of breadwinning/caregiver ideologies. Three patterns of money management were identified. Joint pooling, where equality of sharing, control and decision-making was greatest, was associated with higher income levels and availability of personal spending money. The female whole wage system, with minimal control and joint decision-making, was associated only with women with high-level income and minimal personal spending money. The independent managed system was associated with completely separate money management, unequal sharing of money, increased power, inequality in decision-making, and increased personal spending money by the breadwinner. · The pattern of financial allocation adopted had an influence on control and decision-making in the household. In all the systems of financial allocation adopted, women indicated that their partners had a final say in the financial decision-making processes. The study highlights some policy implications of inequality in financial decision-making. Due to the fact that household based analysis assumes that financial decision-making is shared equally in the households, women and children will most of the time lose out when this is not the case. It was therefore recommended that a deeper understanding of household decision-making may help the policy makers and researchers alike to focus on women in a more effective way, for example, by designing empowering programmes that will assist women to be involved in the financial planning and decision making in their households. / Dissertation (MA (Research Psychology))--University of Pretoria, 2005. / Psychology / unrestricted
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Contrasting Constructions of Students' Literacy-Related Experiences at a Historically Black South African UniversityBoughey, Christine Mary January 2002 (has links)
Philosophiae Doctor - PhD / In recent years, many long held assumptions about language and literacy have come to be questioned by so-called "critical" discourses. The result of this questioning at a theoretical level has resulted in a concomitant interrogation of the practices and methodologies intended to develop both phenomena. Situated against the background of this critical questioning, this thesis examines the appropriacy of interventions
designed to develop students' academic literacy at the University of Zululand, a historically black South African University. It does this by asking two questions about students' literacy-related experiences. The first question, "How does the University of Zululand construct students' literacy-related experiences?", is answered using an analysis of Senate and Faculty documents, extant study and course guides and
archived examination papers. In answering the question, the focus is on the identification and exploration of the ideologies which underpin dominant understandings of students' literacy-related experiences. The answer to the second question, "Is there a way to construct students' literacy-related experiences which is different to dominant understandings at the University of Zululand?", uses ethnographic research to support an analysis of students' written texts produced in a first year Systematic Philosophy class to "talk back" to the dominant understanding of students' literacy-related experiences identified as a response to the first research question. The analysis of students' writing is conducted using a systemic functional linguistic framework (Halliday, 1973, 1978, 1994). A systemic framework relates three different kinds of meanings evident in texts (experiential, interpersonal and textual meanings) to the contexts in which those texts are produced. The framework was used because of its potential to account for the form of students' texts by referring to a mismatch
between the expectations of the dominant contexts of culture and situation (the university and the Systematic Philosophy class in which the research was conducted respectively) and the contexts which students themselves use as a reference point.
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Associations between specific ApoE genetic variants and their interactions with environmental factors in relation to the lipid profile of black South Africans / Lize MeadesMeades, Lize January 2014 (has links)
Introduction: Cardiovascular disease (CVD) is the leading cause of global mortality and its prevalence is increasing among black South Africans in spite of their favourable lipid profile. Apolipoprotein E (ApoE) is a well-described risk factor for CVD and certain polymorphisms within this gene alter the lipid profile. The author hypothesised that there are population-specific effects within the ApoE gene that are responsible for the favourable lipid profile observed in black South Africans whose effects are being altered by environmental factors.
Objectives: The main aim of this study was to investigate the associations between specific ApoE single nucleotide polymorphisms (SNPs) and the lipid profile of a black South African population, taking into account certain environmental and phenotypic factors in order to explore the interaction effects between these variables.
Methods: Genotyping within this cross-sectional study (n=1 588), nested within the Prospective Urban and Rural Epidemiology (PURE) study, was achieved using Illumina‘s® GoldenGate Genotyping Assay with VeraCode® technology on the BeadXpress® platform (proprietary multiplex fluorescent hybridisation assays on a bead array substrate) or the Bio-Rad CFX Manager© (version 2.0). The Konelab20i™ auto analyser was used for quantitative determination of serum total cholesterol; high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) concentrations. Low-density lipoprotein cholesterol concentrations were estimated by the Friedewald equation.
Results: All SNPs adhered to the assumptions of the Hardy-Weinberg equilibrium, yet the frequency of the SNPs often differed from that reported in other ethnic groups. The well-reported rs429358 and rs7412 SNPs (as the constituent SNPs of the haplotype-genotypes) presented with the strongest associations with various components of the blood lipid profile in the black South African cohort under investigation. Two gene-environment (rs405509 and rs7412) interaction effects on TG remained significant after conducting post hoc tests. Two genotype-phenotype interaction effects between the rs7412 SNP and body mass index and gamma-glutamyl transferase on the HDL-C concentrations remained significant after conducting post hoc tests.
Conclusions: The variety of associations between these particular SNPs and the blood lipid profile determined in the present cohort strongly indicates that it is integral to any public health investigation into CVD development that these SNPs be investigated. This study further produced greater insight into the biological mechanisms underlying serum lipid and cholesterol concentrations in a black South African population. Therefore, from these results it is evident that the lipid profile of black South Africans is most definitely influenced by not only genetic variations in the ApoE gene and certain environmental factors, but by the interaction between these factors as well. The present study is the largest study to date to investigate the effect of polymorphisms in the ApoE gene on the lipid profile of black South Africans. / MSc (Nutrition), North-West University, Potchefstroom Campus, 2014
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Associations between specific ApoE genetic variants and their interactions with environmental factors in relation to the lipid profile of black South Africans / Lize MeadesMeades, Lize January 2014 (has links)
Introduction: Cardiovascular disease (CVD) is the leading cause of global mortality and its prevalence is increasing among black South Africans in spite of their favourable lipid profile. Apolipoprotein E (ApoE) is a well-described risk factor for CVD and certain polymorphisms within this gene alter the lipid profile. The author hypothesised that there are population-specific effects within the ApoE gene that are responsible for the favourable lipid profile observed in black South Africans whose effects are being altered by environmental factors.
Objectives: The main aim of this study was to investigate the associations between specific ApoE single nucleotide polymorphisms (SNPs) and the lipid profile of a black South African population, taking into account certain environmental and phenotypic factors in order to explore the interaction effects between these variables.
Methods: Genotyping within this cross-sectional study (n=1 588), nested within the Prospective Urban and Rural Epidemiology (PURE) study, was achieved using Illumina‘s® GoldenGate Genotyping Assay with VeraCode® technology on the BeadXpress® platform (proprietary multiplex fluorescent hybridisation assays on a bead array substrate) or the Bio-Rad CFX Manager© (version 2.0). The Konelab20i™ auto analyser was used for quantitative determination of serum total cholesterol; high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) concentrations. Low-density lipoprotein cholesterol concentrations were estimated by the Friedewald equation.
Results: All SNPs adhered to the assumptions of the Hardy-Weinberg equilibrium, yet the frequency of the SNPs often differed from that reported in other ethnic groups. The well-reported rs429358 and rs7412 SNPs (as the constituent SNPs of the haplotype-genotypes) presented with the strongest associations with various components of the blood lipid profile in the black South African cohort under investigation. Two gene-environment (rs405509 and rs7412) interaction effects on TG remained significant after conducting post hoc tests. Two genotype-phenotype interaction effects between the rs7412 SNP and body mass index and gamma-glutamyl transferase on the HDL-C concentrations remained significant after conducting post hoc tests.
Conclusions: The variety of associations between these particular SNPs and the blood lipid profile determined in the present cohort strongly indicates that it is integral to any public health investigation into CVD development that these SNPs be investigated. This study further produced greater insight into the biological mechanisms underlying serum lipid and cholesterol concentrations in a black South African population. Therefore, from these results it is evident that the lipid profile of black South Africans is most definitely influenced by not only genetic variations in the ApoE gene and certain environmental factors, but by the interaction between these factors as well. The present study is the largest study to date to investigate the effect of polymorphisms in the ApoE gene on the lipid profile of black South Africans. / MSc (Nutrition), North-West University, Potchefstroom Campus, 2014
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Tense and aspect taxonomies among Tshivhenda mother tongue speakers of English : implications for Black South African EnglishRalushai, Moses Mpfariseni January 2015 (has links)
Thesis (M. A. (English Studies)) -- University of Limpopo, 2015 / Whereas research has exponentially increased on the understanding of new varieties of English worldwide, there is a paucity of studies that have assessed the degree to which Black South African English is shared and understood across a wider spectrum of the Bantu language speaking communities in South Africa. Because of this, research has been inconclusive on causes and frequencies of identified linguistic properties of the variety. This study investigated the tense and aspect usages in English among mother tongue speakers of Tshivenda learners to develop a taxonomy of characteristic features, sources of production and frequency of occurrence in an educational context. The study focused more attention on the role of mother tongue substrate system which transfers its features to the target language.
After a contrastive analysis of the collected data, learners’ essays and storytelling, the findings of the study indicate that the occurrence of tense and aspect properties among grade 11 learners is first language (L1) induced and rule-governed. Secondly, the results show that the features occur with high frequency, suggesting that they are institutionalised. In particular, the study denotes that expressions of temporal reasoning follow the L1 grammatical structure that favours external formation to the verb phrase; i.e., verbal arguments are preferred to morphological conjugations.
Based on these findings, it is concluded that the logic of temporal reasoning, described as Bantu language logic as developed by Makalela (2004, 2007, and 2013), rather than grammatical forms, is transferred in the production of BSAE tense and aspect properties. When seen in this light, it is evident that BSAE cuts across different Bantu languages of South Africa, it has evolved towards being an endonormative variety, relying on its own internal logic (substrate forms) to be a new and distinct variety of English. In the end, recommendations for more robust and large scale studies in high prestige domains such as the media and institutions of higher learning are made for augmentation of these findings.
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An acoustic investigation of English vowels as produced by English L1 and Setswana L1 foundation phase learnersLe Roux, Maria January 2016 (has links)
This thesis provides a literature review on various topics related to the aims of the research project. In the process of sketching the rationale of this study, the language-in-education policy (LiEP) of South Africa is examined. The reasons why this policy is not successful, is discussed. Emanating from this discussion, the low literacy scores amongst young learners in South Africa are described and possible reasons for this occurrence are cited (Howie, Van Staden, Tshele, Dowse, & Zimmerman, 2012).
The hypothesis that perceptual and articulation training of the vowels of English would enhance young English second language (Setswana first language-speaking) learners? awareness of English vowels, is posited and defended by referring to previous research (Moats, 2007; Trehearne, 2011; Seeff-Gabriel, 2003). In addition, it is stated that increased knowledge of the vowel system of English will improve English second language (EL2) learners? literacy skills (Moats, 2007). In order to explain and discuss the results of the investigations into the literacy skills of the EL2 learners participating in this study, the notions of literacy acquisition, phonological awareness skills, and language acquisition and language learning are examined.
The main aim of this study is to assess the effects of intervention on the auditory perception and articulatory skills of English second language-speaking (EL2) (Setswana L1-speaking) learners in Grade 3, in the production of the monophthongs and diphthongs of the standard variety of South African English, namely White South African English (WSAfE) (Bekker, 2009). The effects are determined by acoustically comparing the vowel spaces of the participants before and after intervention. Therefore, a discussion of acoustic concepts such as the vowel space and the parameters thereof are provided. In addition, the vowels of WSAfE, those of Black South African English (BSAE), and the vowels of Setswana are discussed and compared according to their acoustic features.
The results of this quasi-experimental, comparative study indicate that the vowel spaces of the EL1 and EL2 participants differ markedly before intervention, especially when comparing those of the short and long monophthongs of English. After intervention, the vowel spaces of especially the Experimental group are seen to approximate those of the EL1 participants in the Norm group. Interesting findings concerning the central schwa vowels and the diphthongs were made. These indicate that young EL2 (Setswana L1-speaking) learners do not use BSAE, but use a new? variety of English that is closer to WSAfE (Mesthrie, 2008).
Pertaining to the measurable sub-aims of this study, the phonological awareness skills and literacy skills of the participants are assessed and compared, before and after intervention. The improvement of the phonological awareness skills and literacy skills of the Experimental group, as well as the changes in the vowel spaces that are noted for this group, prove the hypothesis that intervention in the form of additional input concerning the English vowels will enhance second language learners? perception and articulation. This strengthens the opinion that second language learners need quality teaching of the sound system of the language of learning and teaching. / Thesis (DPhil)--University of Pretoria, 2016. / African Languages / DPhil / Unrestricted
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The association between fibrinolysis markers and body composition in black adults in the North West Province of South Africa / Philna EksteenEksteen, Philna January 2014 (has links)
INTRODUCTION - Plasminogen activator inhibitor type-1 (PAI-1) has a known relationship with obesity and more specifically with central obesity. Traditionally the physiological contribution of PAI-1 is seen as an indicator of fibrinolysis with increased PAI-1 levels contributing to decreased fibrinolysis. In more recent years, assays have been developed that not only uses proxy markers, such as PA-1, which is considered to be representative of fibrinolysis , but global assays that report on the global fibrinolytic potential of an individual, often reported as clot lysis time (CLT). Investigations into the relationship of CLT with obesity are scarce. Preliminary evidence shows that the relationship of CLT with obesity may differ from that of PAI-1 with obesity although in depth investigations in this regard are lacking. Therefore, the aim of this study was to investigate the association between fibrinolysis markers (PAI-1act and CLT) and various markers of body composition in the South African Prospective Urban and Rural Epidemiology (PURE) data collected during 2010.
METHODS - Data collected in the PURE study in 2010 were cross-sectionally analysed. The participants (n = 1288) were apparently healthy black South-African men and women 35 years and older, residing in urban and rural settlements in the North-West Province. Experimental methods included anthropometric measurements such as height, weight, hip circumference, waist circumference, skinfolds (triceps, chest, abdominal, thigh and supra iliac skinfolds) and body composition measurements by means of air-displacement plethysmography and biolelectrical impedance analysis. Laboratory analysis of fibrinolysis markers, PAI-1act and CLT were also performed.
MAIN FINDINGS - In men, similarities were seen regarding the relationship between PAI-1act and body composition markers and the relationships observed between CLT and body composition markers. In contrast, in the women more and stronger associations were observed between CLT and body composition markers compared to that observed between PAI-1act and body composition markers. CLT showed a linear relationship with body composition markers where PAI-1act levels plateaued at higher body composition categories. Possible reasons for the observed differences may be related to differences in adipose tissue distribution and sequence of accumulation between men and women. PAI-1 is associated with visceral adipose tissue (VAT) where high amounts of stromal cells are found. In men preferential accumulation of VAT may explain similarities in the relationship of PAI-1act with body composition and that of CLT with body composition. Proportionally less VAT, but more subcutaneous adipose tissue in women may explain the observed increase in CLT compared to PAI-1act levels that plateaued over body composition tertiles and categories.
CONCLUSION - PAI-1act has a stronger association with central obesity while CLT has a stronger association with total body fat. In women PAI-1act and CLT showed different associations with body composition markers, whereas associations of PAI-1act and CLT with body composition were similar in men. PAI-1act is strongly influenced by type of body fat accumulation whereas CLT is associated with obesity independent of type and sequence of body fact accumulation. Significant associations observed between CLT and body composition variables are, therefore, at least in part, independent of PAI-1act. Additional factors such as, thrombin activatable fibrinolysis inhibitor (TAFI), α-2-antiplasmin, plasminogen, prothrombin and fibrin clot structure that influence CLT and are also related to obesity may additionally contribute to the link between CLT and obesity. / MSc (Dietetics), North-West University, Potchefstroom Campus, 2014
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Black Generation Y male students' fashion consciousness and need for uniqueness / Matebello Dieketseng Bethsheba MotaleMotale, Matebello Dieketseng Bethsheba January 2015 (has links)
While there have been numerous studies directed at addressing the female Generation Y cohort’s fashion consumption patterns, there is a dearth of published research focused on male consumer fashion conscious behaviour, especially not that of the African Generation Y (hereafter referred to as black Generation Y) males. There are global indications that contemporary males are engaging in fashion apparel shopping more frequently than ever before. Moreover, unlike past generations, today’s male consumers have become increasingly fashion aware and fashion conscious, taking care of their looks and developing their own fashion style. Born between 1986 and 2005, in 2013, black Generation Y individuals made up approximately 83 percent of South Africa’s total Generation Y cohort and 38 percent of the country’s 52 981 991 population. In 2013, black Generation Y males accounted for an estimated 42 percent of the South African population. As a consequence of its size, the black Generation Y male market segment represents a potentially lucrative and attractive market for apparel retailers and fashion marketers. Marketers are particularly interested in those individuals pursuing tertiary qualifications given that a higher education ultimately acts as a predictor of their higher future spending potential and an indication of their social standing and influence within a society. The aim of this study was to determine the causal relationships between black Generation Y male students’ need for uniqueness, fashion awareness, fashion consciousness and fashion conscious behaviour in order to model the determinants of black Generation Y male students fashion conscious behaviour. A self-administered questionnaire was administered on a single cross-sectional sample of 400 black Generation Y male students at three university campuses in the Gauteng province in 2013 – one from a comprehensive university, one from a university of technology and one from a traditional university. From the administered questionnaires, 213 were completed and returned as usable. The collected data was analysed by means of exploratory factor analysis, reliability and validity analysis, descriptive statistics and correlation analysis. In addition, structural equation modelling (SEM) was applied to test a proposed model derived from the literature. According to the results of the SEM analysis, black Generation Y males’ fashion awareness has a significant positive influence on their fashion consciousness, and their fashion consciousness has a significant positive influence on their fashion conscious behaviour. In terms of the dimensions of the need for uniqueness, unpopular choice has a significant positive influence on black Generation Y male students’ fashion awareness, whilst creative choice has a significant positive influence on their fashion consciousness, and avoidance of similarity has a direct significant positive influence on their fashion conscious behaviour. Furthermore, the findings suggest that their fashion consciousness is reliant on their fashion awareness, which in turn influences their fashion conscious behaviour. Findings from this study will aid marketers’ in better understanding black Generation Y male students’ fashion conscious behaviour. Furthermore, the recommendations provided suggest marketing strategy guidelines tailored at effectively targeting this market segment. / PhD (Marketing Management)--North-West University, Vaal Triangle Campus, 2015
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A diachronic analysis of the progressive aspect in Black South African English / C.M. PiotrowskaPiotrowska, Carolina Monika January 2014 (has links)
Current research into language change and evolution focuses on native varieties of English, Schneider’s (2007) settler strand, but we have little knowledge concerning diachronic changes in non-native varieties of English, Schneider’s (2007) indigenous strand. Such a theory must take into account language contact as well as theories pertaining to Second Language Acquisition. This dissertation describes the diachronic changes which occur in one feature of Black South African English (BSAE), namely the progressive aspect. Current synchronic research on BSAE suggests that the progressive aspect is overextended to include stative verbs. This dissertation aims to evaluate the value of this hypothesis, and determine whether there is evidence of this overextension in diachronic data. In order to observe instances of language change, a diachronic corpus of BSAE was complied. This corpus consisted of data from letters, fiction, and newspapers ranging from the 1870s until the 2000s. Using this corpus, analyses were performed in order to determine the frequency of progressive use, the distribution of aktionsart categories, and which temporal meanings were denoted by stative verbs and activity verbs used in the progressive aspect. These analyses were then repeated for data taken from the historical corpus of White South African English (WSAE), in order to ascertain whether changes in BSAE developed parallel to WSAE, or if it follows its own trajectory of change. One further analysis was conducted on BSAE: the aspectual categories of 71 state verbs were analysed in order to determine whether speakers of BSAE indeed do overextend the –ing progressive form onto stative verbs describing states and qualities. The results indicate that the frequency of verbs used with the progressive aspect increases for BSAE, but decreases for WSAE. The comparison of aktionsart distribution indicates that BSAE has a higher overall frequency of communication verbs and accomplishments, while WSAE has a higher frequency of activity verbs, verbs denoting the future, and stative verbs. Achievements are used as expected by both varieties. The analysis of the temporal meanings of stative verbs indicates that speakers of WSAE denote the prototypical short duration associated with the progressive aspect for 74.83% of the verbs, while BSAE uses an extended temporal meaning for 46.86% of the verbs, indicating that speakers of BSAE more likely to overextend the temporal duration of stative verbs than WSAE speakers. The temporal meanings for activities were the same for both varieties; the prototypical short duration is denoted by 77.83% of the total activities in BSAE, and 77.11% of the activities in WSAE. The extended duration in both varieties may be due to language change in general, while the additional temporal meanings for statives in BSAE are a result of substrate transfer. / MA (English)--North-West University, Vaal Triangle Campus, 2015
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