<p>The dissertation work discussed here focuses on the behavioral and physiological aspects of visual sexual signaling in the blue crab, <italic>Callinectes sapidus</italic>. The blue crab has a pair of apposition compound eyes that are relatively acute (1.5 &deg; resolution) for an arthropod. The eyes have two photopigments sensitive to blue (&lambda;_max = 440 nm) and green (&lambda; _max=500 nm) light, allowing for simple color vision. Visual cues and signals are used during antagonistic and sexual communication and primarily involve claw-waving motions. A primary feature of the blue crab morphology is its sexually dimorphic claw coloration; males have blue and white claws and females have red claws. However, despite the potential for interesting color signaling, visual cues have typically been considered non-important, particularly in sexual communication where chemical cues have dominated blue crab signaling studies. </p><p>In a series of experiments designed to simultaneously test the role of visual cues in mating behavior and blue crab color vision, I tested males' responses to photographs of females with differently colored claws. I found that photographs of females elicited male courting behaviors. I also found that males preferred females with red claws over those with white or isoluminant (i.e. matched brightness) gray claws. The discrimination of red from isoluminant grey showed the use of color vision in male mate choice. </p><p>In natural populations, the claws of sexually mature females vary from light orange to deep red. To determine males' abilities discriminate between similar colors, I tested male color preferences for red against several shades of orange varying in brightness. Overall, males showed an innate preference for red-clawed females over those with variations of orange claws. However, in tests between red and orange shades similar in both brightness and hue, male blue crabs did not show a distinct preference, suggesting that males are either not able or not motivated to discriminate between these shades. Further, my results suggest that male blue crabs may use a mixture of chromatic and achromatic cues to discriminate between long-wavelength colors.</p><p>After confirming the use of color in mate choice, I focused on the role of claw color in intraspecific communication. To quantify claw coloration, I measured spectral reflectance of claws of a blue crab population in North Carolina. In both sexes, the color of the claw varied with reproductive maturity and may act as a cue of reproductive readiness. Additionally, there was individual variation in claw color which could indicate individual quality. I have modeled the appearance of claw coloration to the blue crab eye and found that these color differences are visible to the blue crab eye and potentially signal gender, reproductive readiness, and/or individual quality. </p><p>After investigating male mate choice, I began investigating visual aspects of female mating behavior. In the blue crab, like many crustaceans, courtship occurs during the female molting cycle and copulation takes place after the female has shed her exoskeleton. In crustaceans and other arthropods with compound eyes, the corneal lens of each facet is part of the exoskeleton and thus shed during molting. I used optomotor assays to evaluate the impact of molting on visual acuity (as measured by the minimum resolvable angle <italic> &alpha _min</italic>) in the female blue crab. I found that visual acuity decreases substantially in the days prior to molting and is gradually recovered after molting. Prior to molting,<italic> &alpha_min </italic>was 1.8 &deg;, a value approximating the best possible acuity in this species. In the 24 hours before molting, <italic> &alpha _min</italic>increased to a median of 15.0 &deg; (N=12), an eight-fold drop in visual acuity. Six days after molting, <italic> &alpha _min</italic>returned to the pre-molting value. Micrographs of <italic>C. sapidus </italic> eyes showed that a gap between the corneal lens and the crystalline cone appeared approximately five days prior to shedding and increased in width the process progressed. This separation was likely responsible for the loss of visual acuity observed in behavioral tests. Since mating is limited to the female's pubertal molt, a reduction in acuity during this time may have an effect on the sensory cues used in female mate choice. These results may be broadly applicable to all arthropods that molt and have particular importance for crustaceans that molt multiple times in their lifetime or have mating cycles paired with molting.</p> / Dissertation

Many species use color during courtship displays, with the more colorful individuals often selected as potential mates. Female blue crabs, Callinectes sapidus, display prominent red markings on their chelipeds, which is absent in males. I tested the hypothesis that females use this sexual dimorphism as an effective signal to potential mates. Body size was positively correlated with size of the colorful pattern on the crusher dactyl. Digital imaging techniques were used to examine and quantify a pattern of coloration in the female blue crab. Morphometric measurements were made using digital images of the carapace and chelae of crabs collected along the Gulf of Mexico coast in Galveston, Texas. Color complexity was examined on digital images of the chelae using Adobe? Photoshop? and Image J. Specific wavelengths were selected and their presence within the attribute quantified and evaluated. To determine whether male blue crabs prefer more colorful females, males were given a choice between females of different female coloration. Males displayed more often and directed more courtship displays towards the more colorful females. I hypothesize that male blue crabs use cheliped coloration as a visual cue for mate selection.

Thesis (M.S.)--University of North Carolina at Wilmington, 2003.

Environmental threats to fisheries are a rising concern for fishery managers. Gulf of Mexico hypoxia is particularly concerning. In order to properly manage Gulf fisheries, the sustainability of species stock and its natural capital values should be analyzed. As an empirical effort, I use blue crab in Louisiana waters as a case study to build a bioeconomic model of blue crab under hypoxia. I apply the natural capital valuation approach to estimate the blue crab value function under hypoxia in order to assess blue crab sustainability. I find that blue crab management in Louisiana under hypoxia is not sustainable, being less so under increasing hypoxia severity. In order to manage blue crab fisheries more stably under hypoxia, regulatory agencies along the Mississippi River should continue current efforts to reduce hypoxia, and take steps to sustain more blue crab stocks by management regimes reflecting the severity of hypoxia.

In the recent years, environmental regulations enforced by federal,state, and local agencies have increasingly addressed water quality issues through progressively more stringent regulations. These regulations have raised concerns in the blue crab industry because processors are now subject to regulations under which new processors are unable to meet the effluent limitations with current processing techniques. This study focuses on the mechanized processing of crab claws. Currently, processors use a brine bath, referred to as the Harris Claw machine, to separate crab claw meat and shell, nd this process yields a waste water which is significantly high in pollutant strength, and is untreatable by biological methods due to the toxicity associated with the high chloride concentrations found in the waste stream. Several alternative crab claw processing systems were developed and evaluated in terms of the meat product yield, the meat product quality, and the wastewater characterization. Two alternatives involved the use of dense media, a 22.5% Staley 1300 corn syrup solution with 5.0% salt and a 30.0% Staley 1300 corn syrup solution, to separate the crab claw meat and shell. These methods, in full scale tests, produced meat yields comparable to that of the brine solution and improved the overall taste of the meat product. However, the effluents had significantly higher BOD5 concentrations. Another alternative to the Harris Claw machine, involved the design, characterization, and testing of a hydraulic separator system (HSS). The HSS was tested on a small scale, but was found to have a meat yield comparable to the Harris Claw machine. The HSS significantly improved the flavor of a final meat product, although the HSS meat product had a significantly lower shelf life than the Harris Claw machine meat product. The waste water quality was improved, because the HSS eliminates the problems associated with a high chloride ion concentration and potentially reduces overall water consumption. / Master of Science

The process wastewater from three blue crab processing plants in Virginia was characterized. Most of these effluent streams were highly concentrated. For example, the retort water from the cookers exhibited the following concentrations: BOD₅ = 14,000-29,000 mg/L, TSS = 650-6,200 mg/L, TKN-N = 2,500-4,000 mg/L, NH3-N = 70-160 mg/L and Total Phosphorus= 100-185 mg/L. One process effluent contained chloride concentrations exceeding 100,000 mg/L. Analysis of production and effluent characterization data from two mechanized plants revealed that wastewater volumes and concentrations varied significantly between the two plants. Both plants exhibited highly variable daily and seasonal production. Treatability studies were completed on the process wastewater because discharge limits exist for TSS, Oil and Grease and in some cases BODs and ammonia. Settling and filtration studies were performed on these effluents. The organics and nutrients in the wastes were highly soluble and limited reduction of COD (18-65% reduction), BOD₅ (9-49% reduction), TKN-N (9-62% reduction), and TP (10-66%) was achieved by filtration. Acidification of the concentrated effluents caused some coagulation of the contents. Coagulation by pH adjustment was most effective between pH 3.0-4.0. TSS removals of 76- 93%, and BOD5 removals of 6-30% were achieved by pH adjustment, thereby showing promise as a pretreatment method. Anaerobic biological treatment systems appear promising for the treatment of wastewater from blue crab processing plants (effluent BOD₅s ranged from 150-420 mg/L), especially for cooker effluent. However, there is concern that salt from the Harris Claw operation will decrease anaerobic treatment performance and that effluent NH3 and TSS levels will need to be further reduced to meet permit limits. Pretreatment of process wastewater by means of pH adjustment did not improve anaerobic treatability. / Master of Science

Hard clams, Mercenaria mercenaria, are slow-moving organisms that are heavily preyed upon by both blue crabs and knobbed whelks in coastal Georgia. Hard clams are unable to escape from these predators, and when found, are commonly injured and/or consumed. Thus, their best survival strategy is to avoid their predators. In this study, we compared changes in clam behavior when exposed to blue crab and knobbed whelk predators. Clams reduced their feeding time when exposed to crabs and whelks, exudates from these predators, and to injured conspecifics. In a field experiment, we compared clam survival when caged predators where near clam beds vs. controls with empty cages. Clam survival was significantly higher when caged crabs or whelks were near, suggesting that clams detected these predators, reduced their feeding time, and were less apparent to ambient consumers. In lab behavioral assays, clams were less responsive to blue crabs in turbulent flows, and in the field, turbulence reduced the distance clams reacted to blue crabs. Previous studies have shown that blue crabs turbulence also diminishes blue crab foraging efficiency, and we conducted a field experiment to determine how turbulence affected clam-crab interactions. Our results suggest that predation intensity is greatest at intermediate turbulence levels, and lowest in flows with low and high turbulence levels. We attribute this pattern of predation intensity to differential effects of turbulence on the sensory abilities of crabs and clams. That is, in low turbulent flows, clams have a sensory advantage over crabs, and initiate avoidance behaviors before they are detected. However, as turbulence increases, clam perception diminishes faster than crabs, switching the sensory advantage to crabs, and making clams more vulnerable to consumers. In highly turbulent flows, crab perception declines at a rate faster than clams, and the sensory advantage returns to clams.

This research examined effects of hypoxic environments on blue crabs, Callinectes sapidus in an estuarine environment. Hypoxic conditions were treated as a multiple stressor involving low dissolved oxygen (D.O.), increased carbon dioxide (hypercapnia), and low pH concurrently. The objectives were to: 1) identify hypoxiahypercapnia by monitoring D.O. and pH as an indicator of hypercapnia in shallow regions of the York River, 2) measure blue crab abundance, and 3) describe blue crab responses to hypoxiahypercapnia via field work at Taskinas Creek and lab measurements of respiration. Ambient D.O. and pH were positively correlated in the Taskinas Creek and York River sites (r= .73). Crab abundance (CPUE) was not significantly different among D.O. and pH ranges. It was concluded that hemolymph blood lactate concentration was not considered a good in situ biomarker for exposure to hypoxickypercapnic conditions. Oxygen uptake was not significantly different between normoxic and hypoxic conditions but was significantly affected by pH.

Few empirical studies exist that compare regulation (R) and fishing crime (VL). The lack of information about R and VL effects stakeholder decision-making. Crime weakens conservation efforts and creates false baseline data. This furthers R and the cycle repeats. The purpose of this correlational study was to determine the statistical association between the number and type of annual commercial blue crab R and VL of the same. The Pearson's R correlation was used to analyze the data because it demonstrated the strength of each relationship. This quantitative study was grounded in enforcement theory. The data was public record and consisted of the number of R and VL issued yearly from the General Assembly of a Mid Atlantic's State Department of Natural Resources (MD-DNR). The intent was to correlate multiple decades, but the earliest available VL data began in 2009. The analysis uncovered divergent patterns. The correlation coefficient of 0.79644 confirmed laws from 2009 correlated positively with 2010 violations. Further analysis revealed a negative correlation for 2010 and 2011 that was indicated by a negative correlation coefficient of -0.3588 and -0.166. The mean average of VL was 12.5%. As restrictions keep increasing, the economic impact on local communities is substantial. This research has the potential to effect positive changes in restrictive harvest practices, record keeping of VL by Natural Resources of this Mid Atlantic State, and harvest reporting practices by crabbers. Sharing the findings with industry stakeholders may stimulate dialogue among stakeholders that answers why one type of regulation was violated more than another, encourage compliance by industry users, and improve conservation efforts to proliferate blue crab. This research contributes to future investigation of often-neglected variables that compromise conservation of blue crab.

This dissertation investigated ingress of postlarval blue crabs Callinectes sapidus to the Newport River estuary, North Carolina, USA. Data from C. similis, Menippe mercenaria, Pachygrapsus transversus, and Arenaeus cribrarius are included in some chapters for comparison. Changes in tolerance to low salinity were examined by: 1) exposing postlarvae (megalopae) collected in coastal and estuarine areas to a range of salinities and 2) determining the cue that stimulates acclimation of coastal megalopae to low salinities, the time to acclimation, and the decrease in salinity necessary for acclimation. Coastal megalopae were less tolerant to salinities of 5 and 10 than megalopae from the estuary. Coastal megalopae became acclimated to low salinities within 12 h when salinity was reduced from 35 to 31. Spatial patterns in abundance during ingress were investigated simultaneously in coastal and estuarine areas. Coastal distributions were determined using nighttime surface plankton tows at slack water after ebb tide and slack water after flood tide on four nights; two each during spring and neap tides. Estuarine distributions were determined using nightly settlement on 'hog's hair' collectors. C. sapidus megalopae were most abundant at the coast east of Beaufort Inlet, but settlement was restricted to western channels of the estuary. Species-specific patterns in abundance were maintained during two spring/neap cycles, possibly due to interactions between larval behavior and physical forcing. Biophysical mechanisms of estuarine ingress were investigated by comparing nightly abundance in coastal and estuarine areas with environmental variables. Comparisons were made using cross-correlation and cross-fourier analyses. High estuarine abundances were associated with wind-driven estuarine inflow and nighttime flood tides. The seasonal pattern of estuarine ingress was strongly associated with the seasonal pattern of alongshore wind stress, suggesting that inter-annual variations in atmospheric forcing may determine the yearly abundance of megalopae arriving in estuarine nursery habitats. The effect of sampling interval on annual megalopal abundance estimates was determined using an 11-year dataset of nightly settlement. Variability in abundance estimates increased with increasing sampling interval. Switching from a one day to two day sampling interval resulted in a 20 % decrease in the likelihood of detecting a significant correlation between annual abundance and CPUE in the North Carolina blue crab pot fishery. / Dissertation