The goal of this paper is to explore the way the Race Card is used in the United States and how its use affects the racial climate of the country. The use of the Race Card in politics, courts, and sport is addressed. Research and examples show that the use of the Race Card in these areas has generally negative effects. In most cases the use of the Race Card in these areas not only reinforces stereotypes but also can racialize a situation, which can cause the actual issue to be ignored. Therefore, in order to improve America’s racial climate and ensure the root of issues are being addressed it is important to limit the use of the Race Card to anti-discrimination issues courts.

"Some six months after the death of Margaret Wise Brown in November, 1952, Ellen Lewis Buell stated that she was the author of more than seventy books, and pointed out that with her passing the children's book publishing world had lost one of 'its most prolific writers.' An obituary notice gave the number of her publications as one hundred books 'under own name and pseudonyms.' The pseudonyms--Timothy Hay, Golden MacDonald, Juniper Sage--represented, according to Miss Brown, 'clear-cut writing personalities and distinct styles' differing from each other and from Margaret Wise Brown so greatly that, from the first draft of a book, it was perfectly clear to her just which one of her literary personalities was doing the writing. This remarkable statement, the discrepancy in the count of her books, and a curiosity about a writer who could produce in a life span of little more than forty years such a great number of books, be it seventy or one hundred, are the motivations for this paper. Its purpose is to compile from various sources a literary biography of Margaret Wise Brown and to establish the canon of her writing"--Introduction. / Typescript. / "August, 1959." / "Submitted to the Graduate School of Florida State University in partial fulfillment of the requirements of the degree of Master of Arts." / Advisor: Robert Clapp, Professor Directing Paper. / Includes bibliographical references.

No description available.

Statistical modeling of a nonstationary spatial extremal dependence structure is a challenging problem. In practice, parametric max-stable processes are commonly used for modeling spatially-indexed block maxima data, where the stationarity assumption is often made to make inference easier. However, this assumption is unreliable for data observed over a large or complex domain. In this work, we develop a computationally-efficient method to estimate nonstationary extremal dependence using max-stable processes, which builds upon and extends an approach recently proposed in the classical geostatistical literature. More precisely, we divide the spatial domain into a fine grid of subregions, each having its own set of dependence-related parameters, and then impose LASSO ($L_1$) or Ridge ($L_2$) penalties to obtain spatially-smooth estimates. We then also subsequently merge the subregions sequentially together with a new algorithm to enhance the model's performance. Here we focus on the popular Brown-Resnick process, although extensions to other classes of max-stable processes are also possible. We discuss practical strategies for adequately defining the subregions and merging them back together. To make our method suitable for high-dimensional datasets, we exploit a pairwise likelihood approach and discuss the choice of pairs to achieve reasonable computational and statistical efficiency. We apply our proposed method to a dataset of annual maximum temperature in Nepal and show that our approach fits reasonably and realistically captures the complex non-stationarity in the extremal dependence.

Recent studies suggest adipose tissue plays a critical role in regulating whole body energy homeostasis in both animals and humans. In particular, activating brown adipose tissue (BAT) activity is now appreciated as a potential therapeutic strategy against obesity and metabolic disease. However, the signaling circuits that coordinate nutrient uptake and BAT function are poorly understood. Here, I investigated the role of the nutrient-sensing mTOR signaling pathway in BAT by conditionally deleting Rictor, which encodes an essential component of mTOR Complex 2 (mTORC2) either in brown adipocyte precursors or mature brown adipocytes. In general, inhibiting BAT mTORC2 reduces glucose uptake and de novo lipogenesis pathways while increases lipid uptake and oxidation pathways indicating a switch in fuel utilization. Moreover, several key thermogenic factors (Ucp1, Pgc1α, and Irf4) are elevated in Rictor-deficient BAT, resulting in enhanced thermogenesis. Accordingly, mice with mTORC2 loss in BAT are protected from HFD-induced obesity and metabolic disease at thermoneutrality. In vitro culture experiments further suggest that mTORC2 cell-autonomously regulates the BAT thermogenic program, especially Ucp1 expression, which depends on FoxO1 activity. Mechanistically, mTORC2 appears to inhibit FoxO1 by facilitating its lysine-acetylation but not through the canonical AKT-mediated phosphorylation pathway. Finally, I also provide evidence that β-adrenergic signaling which normally triggers thermogenesis also induces FoxO1 deacetylation in BAT. Based on these data, I propose a model in which mTORC2 functions in BAT as a critical signaling hub for coordinating nutrient uptake, fuel utilization, and thermogenic gene expression. These data provide a foundation for future studies into the mTORC2-FoxO1 signaling axis in different metabolic tissues and physiological conditions.

Kappa-casein has the general function in process of cheese production. Understanding the effect of gene polymorphism of kappa-casein to the nutritional and physical properties of milk may lead to more efficient breeding of dairy cattle. Within this thesis was genotyped by 240 brown swiss and jersey cows determinated using the PCR-RFLP. Then was performed association analysis of the impact of various genotypes on milk production parameters in the program SAS. An association between gene CSN3 polymorphism and milk yield was not statistically significant.

Development of the sympathetic nervous system (SNS) tone onto peripheral organs has been shown to be susceptible to a wide range of external factors, such as temperature. Although it was initially postulated that the sympathetic signal is uniform across the body, there is growing evidence that there can be target-specific sympathetic signals. To date, evidence for a relationship between developmental influences on SNS tone and organ function is purely correlational. An obstacle to investigating the programming of SNS permanently altering physiology is that experimental manipulations of SNS activity during development would impact multiple organ functions simultaneously, which could affect the overall health of the animal and therefore confound interpretation of the results. Here we used brown adipose tissue (BAT) as a platform to define a critical period and identify molecules that contribute to the development of SNS outflow to peripheral organs. In addition, we explored the molecular target-specificity of sympathetic neurons by performing a single-cell RNA sequencing transcriptomic analysis of adult mouse stellate ganglion (SG) in conjunction with retrograde tracing from two of its targets, brown adipose tissue and forelimb. We discovered four molecularly distinct populations of SG neurons that express unique combinations of neuropeptides and receptors, but we did not find evidence of target specificity. The four distinct SG neuronal populations had marker genes that showed unique expression in each population, including genes encoding secreted peptides and receptors of circulating factors. Also, we found that the expression of some of the marker genes differs across the sympathetic chain, which could provide a means for coordinated regulation of SNS responses to specific types of homeostatic challenges.

In two lactation studies reported in this dissertation, it was hypothesized that feeding 35% brown midrib corn silage (BMRCS) and 25% alfalfa hay (dry matter basis) would result in increased dry matter intake (DMI) around peak lactation compared with feeding conventional corn silage (CCS), causing longer peak milk production, and that feeding dairy cows in early lactation a 16% crude protein diet with fair quality alfalfa hay (FAH) in BMR-based diets would maintain milk production, reduce urinary N excretion, and improve N efficiency compared to those fed high quality alfalfa hay (HAH) in CCS- or BMR-based diets. A third experiment was conducted to assess in situ degradation kinetics of BMRCS harvested prior to or at maturity. The first lactation study was performed to determine the long-term effects of feeding BMRCS fed with a high dietary concentration of good quality alfalfa hay in a high-forage lactation diet on productive performance of Holstein dairy cows for the first 180 d of lactation. Feeding BMRCS-based diet did not affect milk production through peak lactation compared with a CCS-based diet; however, cows fed the BMRCS-based diet maintained heavier body weight through peak lactation and longer peak milk production, which resulted in increased milk yield post peak lactation, leading to greater overall milk production and milk protein yield. A second lactation experiment was performed to investigate if early lactating dairy cows fed with the FAH in BMRCS-based diets would reduce urinary N excretion and improve N efficiency compared to those fed the HAH in CCS- or BMR-based diets. Feeding BMR and HAH had better N utilization by decreasing concentrations of urea in blood, milk, and urine. In addition, feeding BMR-based diets decreased urinary N-to-fecal N ratio, and it was further reduced by feeding the HAH, which can represent an environmental advantage over traditional sources of forages in lactation dairy diets. A third experiment assessed in situ DM and neutral detergent fiber degradation kinetics for two new pre-matured BMR varieties (pmBMR1 and pmBMR2) that can be double-cropped by harvesting at tassel, compared with a sole crop mature BMR (mBMR) and CCS harvested at maturity in dry and lactating Holstein dairy cows. The potentially degradable NDF fraction was greater for BMR hybrids compared with CCS with the exception of the pmBMR2, which had the lowest potentially degradable NDF fraction in dry cows. Estimates of ruminal degradability of NDF were greatest for pmBMR1 in both dry and lactating cows. Feeding BMRCS exerted nutritive and environmental benefits when fed with typical Intermountain West lactation dairy diets. Further research is needed to understand interactive aspects of nutrient utilization with other dietary ingredients under different physiological conditions to take full potential benefits of BMRCS.

Two species of fish, Carassius auratus and Ictalurus nebulosus, were subjected to cold acclimation regimes. Acclimation temperatures were slowly lowered to 3°c, then held for a period of time. At various times during this regime, fish were taken out and allowed to spend time in a temperature gradient to determine their preferred temperatures. Carassius were left in the gradient just long enough to determine a measure of the acute temperature preference, while Ictalurus were left in the temperature gradient for longer periods of time to observe any changes that might occur as the fish adjusted to selected temperatures. In both species of fish, lower acclimation temperatures and increased time spent at low temperatures caused a preference for lower temperatures in the gradient.

Historical experiments regarding the influence of low ambient temperatures were often accompanied with the development of detrimental effects. Our laboratory recently published a protocol of mild cold acclimation (5 weeks in 8 ± 1 řC) which induces cardioprotective phenotype in rats (Rattus norvegicus). An important mechanism of cold acclimation is the activation and increase of brown adipose tissue. Besides the nonshivering thermogenesis brown adipose tissue can produce a multitude of autocrine, paracrine, and endocrine factors which might positively influence whole-body metabolism and function of other important organs. The effect of cold acclimation on brown adipose tissue is not however explored very well. The aim of this work was to asses selected parameters of respiration of isolated brown adipose tissue mitochondria of control rats living in 24 ± 1 řC and compare it with that of rats exposed to 8 ± 1 řC for 1 day, 3 days, 10 days, and 5 weeks. Results of this works were 1) the increase in respiration occurs after the 1-day cold exposure and 2) the highest respiration per 1 mg of mitochondrial protein appears to be after the 10 days of cold exposure. Key-words: Brown adipose tissue, mitochondria, respiration, cold acclimation