京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第20968号 / 医博第4314号 / 新制||医||1026(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 長船 健二, 教授 上杉 志成, 教授 岩田 想 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第21165号 / 農博第2291号 / 新制||農||1060(附属図書館) / 学位論文||H30||N5139(農学部図書室) / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 縄田 栄治, 教授 舟川 晋也, 教授 松浦 健二 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

No description available.

Copper tolerant brown rot fungi are able to depolymerize the structure of wood treated with copper or organic wood preservatives. This research used quantitative polymerase chain reaction (qRT-PCR) combined with RNA-seq to explore what genes of the brown-rot fungus, Fibroporia radiculosa, are expressed when the fungus is overcoming the wood preservatives and decaying the wood. The preliminary study of ACQ-treated wood indicated that the hydrogen peroxide needed for wood decay to proceed may come from AAOX (aryl alcohol oxidase), with oxalate regulation by ODC2 (oxalate decarboxylase), and copper regulation by COP (copper resistance P-type ATPase pump). The principal study measured the expression of ten genes at early, mid, and late stages of decay in wood treated with azole, copper, quat, ACQ, CA, plus untreated. Both AAOX and LCC (laccase) were often expressed at their highest levels early in the decay stages, thus either one or both could be involved in early Fenton chemistry. Expression levels of ICL (isocitrate lyase) and GLOXDH (glyoxylate dehydrogenase) were also highest in early decay stages. Of great interest was the complete lack of expression of the COP gene on copper-treated wood at any decay stage. The most surprising and significant result is the impact the quat-treatment had on the metabolism of the fungus, and lack of impact of the azole-treatment. This research indicates that it is the quat that provides the greatest inhibition of F. radiculosa, more so than the copper. Based on RNA Seq, the total number of genes that were up- or down-regulated on the copper-treatment was 473, with 293 on the quat-treatment, and 185 on the azole-treatment. There were a number of genes with unknown protein functions highly expressed. These data distinctly show that gene expression profiles of F. radiculosa are altered by different wood preservative compositions and the duration of wood decay. These genes and this data needs further analysis and study in order to meet the long term goal of understanding the mechanism of copper-tolerance inFibroporia radiculosa.

The brown bear (Ursus arctos) is a generalist omnivore that occupies diverse habitats and displays high levels of behavioral plasticity. Plasticity in foraging behavior can result in variable diets across a population, and allow individuals to use a greater variety of resources. The distribution of food in space and time may also be an important factor in determining brown bear resource selection as it can greatly influence foraging efficiency. Our objectives were to determine if seasonal brown bear home ranges were influenced by temporal availability and spatial distribution of resources and if deviation from an optimal diet influenced brown bear body condition. Understanding how the spatial and temporal distribution of food influences brown bear space use and the relationships between behavioral plasticity and the ability of populations to persist in dynamic environments could provide additional insights into the eco-evolutionary advantages of variable foraging behavior and ultimately population resilience.

No description available.

Obesity is a growing problem that impacts both adults and children. Obesity is linked to the development of unfavorable health outcomes like excess fat accumulation in the liver, a problem known as non-alcoholic fatty liver disease (NAFLD). Brown adipose tissue (BAT), a thermogenic body fat that can be turned on by cold, produces heat by consuming circulating lipids and glucose in a futile cycle. Less active cold-stimulated BAT is linked to obesity and type 2 diabetes in adults but whether this relationship exists in children is unknown. In rodents, increases in BAT activity are associated with reductions in NAFLD, effects that may be mediated through changes in the gut microbiome and reductions in peripheral serotonin. Whether the gut microbiome and serotonin play a role in regulating BAT activity in adults and children is not known. In this thesis, we have utilized magnetic resonance imaging (MRI) proton density fat fraction (PDFF) to assess BAT in the supraclavicular (SCV) region after three hours and one hour of whole-body cold exposure in adults and children, respectively. In 60 adults (aged 18-57 years), we examined whether there is a relationship between cold-stimulated BAT activity and liver fat (assessed via MRI) and whether gut microbiota plays a role in connecting these two tissues. In children, we investigated, BAT activity after whole-body cold exposure in 26 boys (aged 8-10 years). We also explored if BAT activity was different between boys with and without overweight/obesity. Finally, in young boys, we measured the levels of serotonin in platelet-poor plasma and its metabolic end product 5-hydroxyindole acetic acid (5-HIAA) in the urine. We explored if these measures of circulating serotonin were related to cold-stimulated BAT activity and if they were different between boys with and without overweight/obesity. The findings from this research indicate that higher cold-stimulated BAT activity is associated with lower liver fat in adults, but that this relationship is unlikely mediated through changes in the gut microbiota. Additionally, boys with overweight/obesity have lower cold-stimulated BAT activity and lower 5-HIAA in their urine compared to those with normal weight. Moreover, circulatory serotonin is negatively related to total adiposity. However, circulating serotonin is not related to cold-stimulated BAT activity in this cohort. These findings are important as they indicate for the first time that increasing BAT activity in adults and children could potentially be a new avenue for the treatment of NAFLD and obesity. / Thesis / Doctor of Philosophy (PhD) / Obesity is a risk factor for the accumulation of extra liver fat, a problem known as non-alcoholic fatty liver disease (NAFLD). Brown adipose tissue (BAT) is a kind of body fat that rather than storing calories like white fat burns calories when switched on by cold. Studies in adults have shown that people with obesity and type 2 diabetes have less active BAT suggesting switching it on may be helpful to promote weight loss and lower glucose. However, whether this relationship exists in children is not known. In rodents, increased BAT activity has also been linked to reductions in NAFLD, effects that might involve a hormone called serotonin, or changes in the gut microbiome but whether this is important in children and adults is also not understood. In this thesis, we utilized magnetic resonance imaging (MRI) to examine BAT activity after whole-body cold exposure in adults (3 hours) and children (1 hour). In 60 adults (aged 18-57 years), we report that higher cold-stimulated BAT activity is linked to NAFLD, but gut microbiota does not seem to play a role in this relationship. In 26 boys (aged 8-10 years), BAT is less responsive to cold in boys with overweight/obesity compared to boys with normal weight. Additionally, serotonin is lower in boys with overweight/obesity compared to boys with normal weight. These findings suggest that increasing BAT activity in adults and children could potentially be a new avenue for the treatment of NAFLD and obesity.

No description available.

This study examines the evolution of the popular images of John Brown and Thomas "Stonewall" Jackson. It begins by analyzing the historiography of each man. The second and third chapters are biographies of each man. The fourth, fifth, and sixth chapters examine the popular images of the two men in print media, visual media, and monuments. This thesis concludes with appendices which contain reproductions of songs, photographs, and paintings referred to in the chapters. This study finds that the myth of the Lost Cause has kept Thomas Jackson's popular image consistently positive and heroic since his death in 1863. At the same time, this myth has contributed to an ever-changing image of Brown, though other issues, such as race and terrorism, have played significant roles as well. Brown has at various times been considered a madman, a saint, and merely a product of his times. Because the Lost Cause continues to pervade popular memory of the Civil War, Jackson's image is unlikely to change quickly. Because race and the fear of terrorism continue to pervade American society, Brown's image is likely to remain controversial.

BACKGROUND: Brown adipose tissue (BAT) is a thermoregulatory tissue that may have a positive influence on metabolic health by improving glucose homeostasis, reducing adiposity and increasing energy expenditure. It is enriched with uncoupling protein-1 (UCP1) and therefore produces heat by uncoupling oxidative phosphorylation from ATP production. It has long been known that infants are born with BAT, however, only recently has BAT been reported in children and adults. In humans, BAT is predominantly located in the supraclavicular (SCV) region, however there are smaller depots in the peri-renal and mediastinal areas. BAT has primarily been studied in humans using Positron Emission Tomography/Computed Tomography (PET/CT). Such studies have demonstrated that children appear to have a higher prevalence of BAT than adults, however this methodology is not suitable for widespread research in healthy children. Therefore non-invasive methods that accurately measure BAT are required. The factors influencing BAT activity are of interest as this tissue may act as a desirable therapeutic target for metabolic diseases such as obesity and diabetes. PURPOSE: The purpose of this thesis project was two fold; Part 1 involved the examination of SCV skin temperature as a measure of BAT activity in children and the objective of Part 2 was to determine if SCV skin temperature had a relationship to body composition and physical activity in children. METHODS: We recruited 33 pre-pubertal boys (ages 8-10) to this cross-sectional study. The study included 3 visits, in which we measured lean mass and fat mass with dual-energy X-ray absorptiometry, resting energy expenditure (REE) before and after a 30 minute 12ºC cold exposure with indirect calorimetry, objective physical activity with an accelerometer and SCV temperature (measured every 2.5 minutes during a 30 minute, 12ºC cold exposure) with an Infrared Thermal Camera. Lean mass and Fat mass were quantified as lean mass index (LMI) and fat mass index (FMI). Physical activity was quantified as total accelerometer counts per minute (CPM) and minutes of moderate to vigorous physical activity (MVPA) per day. For Part 1, we assessed the precision of IR-thermal imaging of SCV skin temperature by examining the reproducibility of eight skin temperature outcomes over two trials. Furthermore, we assessed the accuracy of these eight skin temperature outcomes by investigating their association with energy expenditure. For Part 2 we evaluated the relationship of FMI, LMI and physical activity (MVPA) with SCV skin temperature in a multivariate analysis. RESULTS: Following these analyses, post-cooling skin temperature had the highest reproducibility of the eight skin temperature outcomes (intraclass correlation coefficient of 0.95, p<0.001) and was also significantly correlated with energy expenditure (Pearson correlation=0.392 p=0.032). Therefore, we used this outcome measure when examining the relationship between SCV skin temperature, body composition and physical activity. Fat mass index (FMI) was inversely related to post-cooling SCV skin temperature (β= -0.125, p<0.001). Minutes of moderate to vigorous physical activity and lean mass index (LMI) were not related to post-cooling SCV skin temperature. CONCLUSION: This study determined that post-cooling SCV skin temperature may be useful for detecting BAT in children and it is inversely related to adiposity. / Thesis / Master of Science (MSc)