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Gender Impacts of Molecular-Assisted Breeding: The Case of Insect and Disease Resistant Cassava in NigeriaMarimo, Pricilla 26 June 2009 (has links)
Cassava is the main staple crop in Nigeria. Using primary data from four south eastern states in Nigeria, the study assessed the gender impacts of improved cassava varieties. Comparative statistical analysis reveals that total female labor is higher than total male in cassava production, processing and marketing. Women spend more labor days than males for planting, weeding, harvesting, marketing and processing. The total female family labor is higher for adopters of new improved cassava varieties. There is however lower family labor input for both male and female adopters for clearing and plowing which are normally done by men.
Significant determinants of female labor supply are number of children in the household, percent of females in the household providing labor on the farm, area under improved cassava varieties and total land area. There is a positive unexpected relationship between total female labor supply and number of children.
For each of the decision making variables, there is a significant association between the gender of the spouse and the decision made except for the decision on family labor allocation. Probit results show a significant decrease in the probability that the wife makes the decision for family labor allocation, what inputs to buy and borrowing and traditional cassava income control with adoption.
Results indicate that both men and women spend their income on services directly linked to the household's welfare. More than half of the women ranked food as number one. / Master of Science
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Estudo da poda da mandioca (Manihot esculenta Crantz)Aguiar, Eduardo Barreto [UNESP] 28 March 2011 (has links) (PDF)
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aguiar_eb_dr_botfca.pdf: 799841 bytes, checksum: 76febcd3057aea23b05ef3cdca2c3afa (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A poda da parte aérea da mandioca é prática comum nos cultivos comerciais destinados à industrialização. Vem sendo praticada principalmente por possibilitar o controle das plantas infestantes com herbicidas no segundo ciclo vegetativo. Seus efeitos no desenvolvimento das plantas e na produtividade de raízes de mandioca ainda não são claros e resultados controversos são encontrados na literatura. Com o objetivo de estudar os efeitos da poda foram realizados seis experimentos, em dois ambientes: Botucatu, experimentos 1, 3 e 5 e em São Manuel, experimentos 2, 4 e 6. Os experimentos 1 e 2 tiveram como causas de variação cinco variedades de mandioca cultivadas com e sem poda. Os experimentos 3 e 4 avaliaram nove épocas de poda frente a uma testemunha conduzida sem poda. Os experimentos 5 e 6 tiveram como causas de variação quatro densidades de plantio avaliadas com e sem poda. Concluiu-se que: a poda altera a produtividade de matéria seca de raízes de maneira distinta considerando a variedade e o ambiente; a poda anterior ao período de repouso fisiológico ou após o início do segundo ciclo vegetativo reduz a produtividade de matéria seca de raízes; em altas densidades de plantio, a poda reduz de maneira significativa a produtividade de matéria seca de raízes. Desse modo, a poda da parte aérea da mandioca não deve ser recomendada de maneira genérica, devendo considerar entre outros, os fatores avaliados no presente estudo / Cassava pruning is common in commercial crops destined for industrialization, a practice mainly aimed at enabling weed control with herbicides in the second growth cycle. Its effects on plant development and cassava yields remain unclear and controversial results are reported in the literature. With the objective of studying the effects of pruning, six experiments were conducted in two environments: in Botucatu, experiments 1, 3 and 5, and in São Manuel, experiments 2, 4 and 6. In experiments 1 and 2, the established variables were five varieties of cassava cultivated with and without pruning. Experiments 3 and 4 evaluated nine pruning dates compared with control cultivated without pruning. The established variables in experiments 5 and 6 were four planting densities, evaluated with and without pruning. It can be concluded that: cassava pruning causes distinct alterations in the productivity of dry root matter, depending on the variety and culture environment; pruning before or after the period of physiological rest or after the onset of the second growth cycle reduces the productivity of dry root matter; and pruning in high planting densities significantly reduces the productivity of dry root matter. Thus, cassava pruning cannot be recommended as a general practice, as currently occurs, and the factors evaluated in this study should be taken into consideration
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Development of cassava (Manihot esculenta Crantz) cultivars for resistance to cassava mosaic disease in Zambia.Chikoti, Patrick Chiza. January 2011 (has links)
Despite the increasing number of farmers growing cassava in Zambia, yield per hectare has
remained low at 5.8 t ha-1. The major constraints contributing to low yields are pests and
diseases of which cassava mosaic disease (CMD) caused by East Africa cassava mosaic virus
(EACMV), Africa cassava mosaic virus (ACMV) and South Africa mosaic virus (SACMV) is the
most important. Breeding of cassava is restricted by limited information on viruses and
associated satellites, and farmer preferences. Most of the farmers cannot manage to institute
control strategies that require buying of chemicals. The most feasible option remains improving
existing cultivars through resistance breeding. The study therefore was conducted to: i)
establish farmers’ perception and knowledge of CMD; ii) to identify viruses of cassava occurring
in Luapula province; iii) evaluate the performance of local and improved cultivars for agronomic
traits; iv) evaluate the performance of F1 progenies for CMD resistance; and v) determine
general combining ability and specific combining ability for CMD resistance. The studies were
carried out between 2008 and 2011 at different locations in Zambia. The information generated
was important in formulating a local breeding strategy for CMD resistance.
A participatory rural appraisal and a structured survey was conducted in Mansa, Samfya and
Mwense districts in Luapula province involving farmers to ascertain farmers’ perceptions of
CMD. The results of the study showed that the majority of the respondents (97.6%) were not
aware of CMD. Most of the farmers grew landraces on small pieces of land. Although, the
cultivars (local and improved) were widely grown, they were susceptible to CMD. The farmers
preferred cultivars with high yielding and early bulking characteristics among others.
A CMD survey conducted between April and May 2009 in Samfya, Mansa, Mwense,
Kawambwa and Nchelenge districts in Luapula province established East Africa cassava
mosaic virus (EACMV), and Africa cassava mosaic virus (ACMV) as the most prominent viruses
in the area. Symptoms of satellites were also observed in the farmers’ fields in most of the areas
visited. Satellite II and III were detected in leaf samples. The CMD incidence (59.1%) and
severity (2.4) was moderate across the districts surveyed. The CMD symptoms on the cassava
plants were variable with plants showing mild and severe symptoms characterised with
narrowing and reduced leaf blades. The transmission of CMD infections was mainly through
cuttings rather than via whitefly infection which means that most of the planting materials used
by the farmers were infected.
Evaluation of cassava cultivars for CMD resistance was conducted in 2009/2010 and 2010/11
seasons at Mansa Research Station in Luapula province using a 4 x 4 α lattice design. Both
introduced and locally grown cultivars had significant (P<0.001) differences in their reaction to
CMD. Bangweulu, Namuyongo, Kalaba, Chikula, Mwakamoya, Chila7 and Chila11 were the
most susceptible genotypes. Mweru, Tanganyika, and Nalumino were moderately tolerant to
CMD.
Eight hundred F1 genotypes developed using a North Carolina II mating design were evaluated
in a 4 x 5 α lattice design in 2011 at Mansa Research Station, Luapula province to determine
combining ability for reaction to CMD, yield and yield components. The plants were harvested 7
months after planting (MAP). Significant (P<0.001) general combining ability and specific
general combining ability were recorded for CMD. The SCA effects were more important for
CMD than GCA effects suggesting that non-additive gene action was more prominent than the
additive gene action in determining CMD reaction. Parent lines with desired significant, negative
GCA effects for reaction to CMD were Bangweulu, Kampolombo, Nalumino and TME2.
In general, the survey and participatory rural appraisal established CMD as one of the
constraints to cassava production and created a basis for the research study. The findings
indicate opportunities that exist in creating genotypes with tolerance to CMD. The study
identified cassava lines with resistance to CMD. The lines that expressed the above trait should
be selected and tested further for release to the farmers in Zambia. Since the clonal evaluation
trial was harvested at 7 MAP, there is need to investigate further for earliness trait in best
performing lines in different locations. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.
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Cassava breeding through complementary conventional and participatory approaches in western Kenya.Were, Woyengo Vincent. January 2011 (has links)
Participation of farmers in plant breeding programmes has been reported to increase
breeding efficiency. Farmers’ participation bridges the gap between variety development and
dissemination and provides an opportunity for farmers to select varieties they prefer. The
breeders on the others hand learn more about the farmers’ preferences and the environment
in which the new varieties will be grown. However, the advantages of participatory breeding
can best be realized when farmers’ indigenous technical knowledge (ITK) and experience
complement the breeder’s scientific knowledge and skills. Cassava (Manihot esculenta
Crantz) is a clonally propagated crop grown in diverse environments by small scale farmers
for subsistence. Information on the roles of farmers and breeders at various stages of
breeding and their ability to effectively participate in breeding programmes is limited. The
objectives of this study were to determine: (1) cassava farmers’ preferences, production
constraints and systems; (2) farmers’ selection criteria of cassava varieties; (3) genetic
inheritance of farmer preferred traits; (4) how farmers and breeders complement each other
at all stages and activities of cassava breeding.
Participatory rural appraisal was conducted in three purposefully sampled districts of western
Kenya based on ethnicity and agro-ecology. The results reveal that cassava is
predominantly grown by small scale farmers with mean land size of 1.6 ha mainly under
mixed cropping system for subsistence. The storage roots are eaten either after boiling or
processing to flour. The majority of farmers (over 60%) are aware of the improved varieties
but adoption rate is low (18% in some districts). The effects of pests and diseases, and the
lack of high yielding varieties, capital, land, and disease free planting material are the most
important constraints to cassava production. Farmers prefer tall, high yielding varieties that
are resistant to diseases and pests, early maturing and long underground storability of
harvestable storage roots. The districts surveyed significantly differed in popularity of
utilization methods, traits preferences and relative ranking of the production constraints
indicative of differences in ethnicity and agro-ecology.
Three farmer groups from the three districts selected in western Kenya were used to study
farmers’ variety selection criteria based on their own indigenous technical knowledge (ITK).
The groups evaluated 15 (10 landraces and five improved) popular cassava varieties with
concealed identities on their farms. The results revealed that farmers have effective methods
of selecting varieties for most of their preferred traits. However, ITK alone cannot be used to
evaluate all the important traits, such as cyanide content.
The genetic inheritance of farmer preferred traits was determined through a genetic study.
Six landraces and four improved varieties popular in western Kenya were crossed using the
North Carolina mating design II to generate 24 full-sib families. The 24 families, represented
by 40 siblings each, were evaluated at two sites, Kakamega and Alupe research station
farms, in a 24 x 40 a-lattice design. General combining ability (GCA) and specific combining
ability (SCA) mean squares were significant (P<0.05) for all traits evaluated except dry
matter content and cyanide content. However, non-additive gene action predominated over
additive gene for cassava mosaic disease (CMD) resistance, height to first branching, total
number of storage roots per plant and fresh storage root yield in all environments. The best
crosses were not necessarily obtained from parents with high general combining ability
confirming the presence of non-additive gene action. The best performing parents per se did
not necessarily have high GCA effects implying that selection based on the per se
performance of parents may not always lead to development of superior hybrids.
The clonal evaluation trial (CET) was established at Alupe research station and evaluated by
the breeder and farmers from two districts independently. Three selection criteria were
tested to determine the most appropriate approach to selection of varieties that meet both
farmers’ and breeder’s preferences. The selection criteria were; farmers’ independent
selection index (SI) derived from farmers’ selection criteria from each district, breeder’s
negative selection and independent SI, and a participatory SI which combines farmers’ and
breeder’s selection criteria. There was 14% overlap among the top 100 varieties selected by
farmers from all districts and the breeder when independent SI were used. However, there
was 49% overlap among the top 100 varieties selected by farmers using participatory SI and
the breeder’s SI. The farmers and the breeder have a role to play in the variety development
process. Varieties with traits preferred by both the farmers and the breeder are likely to
enhance breeding efficiency and effectiveness. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.
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Breeding and evaluation of cassava for high storage root yield and early bulking in Uganda.Tumuhimbise, Robooni. January 2013 (has links)
Cassava (Manihot esculenta Crantz), is the world’s most widely grown starch storage root crop. It is a principal food staple in sub-Saharan Africa where it accounts for approximately one-third of the total production of staple food crops. It plays a key role as a food security and an income-generating crop for millions of smallholder farmers. In Uganda, cassava ranks second to bananas (Musa spp.) in terms of area occupied, total production and per capita consumption; however, nearly 5% of the total population experiences hunger with the prevalence of food energy deficiency at the country level standing at 48%. Cassava is a crop with high potential to alleviate food shortages and energy deficiencies, owing to its unique advantages of producing acceptable yields and starch on infertile soils amidst erratic rainfall, when most other crops would fail. Hoewever, its yield potential has not been fully realised since most of the cassava cultivars grown are susceptible to pests and diseases, low yielding and late bulking. The main objective of the research was to develop high yielding, early bulking cassava genotypes that combine resistance to cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) with farmer preferred traits for cultivation in Uganda. The specific objectives were to: (i) evaluate farmers’ attitudes to and/or perceptions of cassava early bulking, production constraints and cultivar preferences; (ii) determine the extent of genetic variability in storage root bulking and other important traits of selected cassava genotypes; (iii) assess the effects of genotype x environment interaction on early bulking and related traits of selected cassava genotypes; (iv) develop and evaluate cassava F1 families for early bulking in terms of the attainment of early, high fresh storage root yield (FSRY) and resistance to CBSD and CMD; and (v) determine the combining ability and gene action controlling early bulking and yield-related traits, as well as resistance to CBSD and CMD. Through the farmer participatory survey, a number of cassava production constraints were identified, key of which were: diseases, especially CBSD and CMD; lack of early bulking cultivars; rodents and insect pests. Farmers rated early bulking as the second most important preferred trait after FSRY, but suggested that early bulking should be complemented with high dry mass content (DMC), sweetness, high FSRY and resistance to pests and diseases. The analysis of variance of 12 cassava genotypes selected for evaluation in three diverse locations and at five different harvest times indicated significant variation among genotypes, harvest times, locations and their interactions for FSRY and most of the other traits evaluated. Fresh storage root yield and the other traits evaluated were predominantly under the control of genetic variation, indicating that genetic advance would be achieved through hybridisation of the test genotypes. Additive main effects and multiplicative interaction (AMMI) analysis of the data collected at nine months after planting (MAP) indicated a non-significant GEI for early FSRY, but significant GEI for other traits assessed. Eight of the 12 genotypes analysed had relatively low interaction with locations for early FSRY, signifying that these genotypes were relatively stable for early FSRY. Thirty-six F1 families were generated from a 9 x 9 diallel and exhibited a high degree of variation between and within families for all the traits assessed at the seedling evaluation stage. Diallel analysis at the seedling evaluation stage at 10 MAP indicated that additive gene effects were predominant in the expression of early FSRY and most of the other traits analysed. At the clonal evaluation stage, the 36 families were assessed for early FSRY at 8 MAP and this trait together with most of the other traits assessed were found to be predominantly under the control of non-additive gene effects. High mid- and better-parent heterosis for early FSRY was recorded in most families at the clonal evaluation stage with NASE3 x Nyara, Nyara x B11 and NASE3 x B11 recording the highest. Selection from the 36 families at the clonal evaluation stage based on farmers’ top two preferred traits, viz. early bulking for FSRY and DMC, plus resistance to CBSD and CMD identified 50 genotypes that had early FSRY of ≥25 t ha-1 at 8 MAP compared to the best parent, CT1 that had 15.9 t ha-1 at 8 MAP. The selected genotypes also had high DMC and dual resistance to CMD and CBSD. Advancement of the selected genotypes should go a long way towards increasing cassava yield per unit time, reducing food shortages and increasing the income of smallholder farmers in Uganda. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.
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Estudo da poda da mandioca (Manihot esculenta Crantz) /Aguiar, Eduardo Barreto, January 2011 (has links)
Orientador: Sílvio José Bicudo / Banca: Teresa Losada Valle / Banca: José Carlos Feltran / Banca: Marina Aparecida de Moraes Dallaqua / Banca: Ricardo Augusto Dias Kanthack / Resumo: A poda da parte aérea da mandioca é prática comum nos cultivos comerciais destinados à industrialização. Vem sendo praticada principalmente por possibilitar o controle das plantas infestantes com herbicidas no segundo ciclo vegetativo. Seus efeitos no desenvolvimento das plantas e na produtividade de raízes de mandioca ainda não são claros e resultados controversos são encontrados na literatura. Com o objetivo de estudar os efeitos da poda foram realizados seis experimentos, em dois ambientes: Botucatu, experimentos 1, 3 e 5 e em São Manuel, experimentos 2, 4 e 6. Os experimentos 1 e 2 tiveram como causas de variação cinco variedades de mandioca cultivadas com e sem poda. Os experimentos 3 e 4 avaliaram nove épocas de poda frente a uma testemunha conduzida sem poda. Os experimentos 5 e 6 tiveram como causas de variação quatro densidades de plantio avaliadas com e sem poda. Concluiu-se que: a poda altera a produtividade de matéria seca de raízes de maneira distinta considerando a variedade e o ambiente; a poda anterior ao período de repouso fisiológico ou após o início do segundo ciclo vegetativo reduz a produtividade de matéria seca de raízes; em altas densidades de plantio, a poda reduz de maneira significativa a produtividade de matéria seca de raízes. Desse modo, a poda da parte aérea da mandioca não deve ser recomendada de maneira genérica, devendo considerar entre outros, os fatores avaliados no presente estudo / Abstract: Cassava pruning is common in commercial crops destined for industrialization, a practice mainly aimed at enabling weed control with herbicides in the second growth cycle. Its effects on plant development and cassava yields remain unclear and controversial results are reported in the literature. With the objective of studying the effects of pruning, six experiments were conducted in two environments: in Botucatu, experiments 1, 3 and 5, and in São Manuel, experiments 2, 4 and 6. In experiments 1 and 2, the established variables were five varieties of cassava cultivated with and without pruning. Experiments 3 and 4 evaluated nine pruning dates compared with control cultivated without pruning. The established variables in experiments 5 and 6 were four planting densities, evaluated with and without pruning. It can be concluded that: cassava pruning causes distinct alterations in the productivity of dry root matter, depending on the variety and culture environment; pruning before or after the period of physiological rest or after the onset of the second growth cycle reduces the productivity of dry root matter; and pruning in high planting densities significantly reduces the productivity of dry root matter. Thus, cassava pruning cannot be recommended as a general practice, as currently occurs, and the factors evaluated in this study should be taken into consideration / Doutor
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Manipulating morphological traits of cassava to enhance host plant resistance and biological control of cassava green mite in Zambia.Chalwe, Able. 21 November 2013 (has links)
Understanding direct and indirect defense mechanisms that enhance host plant resistance (HPR) and biological control is critical for successful development of an integrated pest management (IPM) approach. Cassava green mite (CGM) (Mononychellus tanajoa Bondar (Acari: Tetranychidae)) is a major arthropod pest of cassava (Manihot esculenta Crantz) in Africa. Strategies to control CGM include HPR and biological control by use of exotic natural enemies particularly the predatory mite Typhlodromalus aripo DeLeon (Acari: Phytoseiidae). The success of the latter depends on continuous survival of the natural enemy which requires suitable host plants and weather conditions. Various plant morphological traits have been recognized as indirect defense mechanisms that enhance HPR to CGM, and/or attract T. aripo in cassava. It was envisaged that integration of HPR and classical biological control approaches through manipulation of such indirect defense traits would lead to a more sustainable management of CGM in view of anticipated climate change. Lack of information on farmers’ perception of CGM and preferred varietal attributes, and gene action controlling the inheritance of CGM resistance also limits success of resistance breeding and adoption of varieties. This research was undertaken to gather information on farmers’ perceptions of cassava varietal attributes and cultural practices in relation to CGM resistance, identify suitable sources of resistance and environments for future breeding; and to determine the nature of gene action controlling CGM resistance and the inheritance of plant morphological traits that enhance the ability of cassava to host and support continuous survival of natural enemies.
High fresh storage root yield (FSRY), high storage root dry mass percentage (SRDM%), earliness combined with extended underground storability, and resistance to foliar pests and diseases are the major factors that influence adoption and retention of genotypes by farmers. Moles, termites and CGM are the most widespread and most damaging pests. However, due to the non-conspicuous nature of CGM, its effects are under-estimated and are given limited attention by farmers. The majority of the farmers are familiar with CGM leaf damage symptoms but they cannot associate them with the actual pest. Participation of farmers in field training and field research activities helps them to know CGM. Crop rotation, intercropping, removal of shoot tips, selective pruning of infested shoots, and burning of cassava fields are some of the ways used by farmers to manage CGM. Farmers associate hairy broad-leaved, tall cassava genotypes and pink leaf pigmentation (anthocyanin) with low CGM damage.
There is substantial genetic variability in the Zambian cassava germplasm for CGM resistance and associated plant morphological traits such as leaf pubescence (Pbs), leaf retention (LR), stay green (SG), tip size (TS), tip compactness, and plant height (PH), stem diameter (StD), SRDM% and FSRY. Genotypes with wide or specific adaptability for these traits have been identified, and should be recommended for general or localized production and for use as sources of desired genes in crop improvement. Genotypes L9.304/147, 92/000, TME2, 4(2)1425, I60/42 and L9.304/175 combine wide adaptability with high levels of resistance to CGM. Genotypes Kapeza, L9.304/147 and 4(2)1425 are able to produce 13-15 t ha ¯¹ at 9 months after planting suggesting their potential for early bulking.
This study has shown that both additive and non-additive gene effects play a role in the expression of CGM resistance and associated plant morphological traits. The best combinations of parents for resistance against CGM were 4(2)1425 x L9.304/147 and Mweru x L9.304/147, while L9.304/147 x I92/000 displayed combined resistance to CGM and cassava mosaic disease (CMD). The resistance of cassava to CGM is positively correlated with Pbs, LR, and TS, SG, PH, StD. Overall, the study has shown that there is wide diversity in the expression of valuable indirect defense traits among genotypes, indicating that there is scope for integration of biological control and host plant resistance for CGM in Zambia. The release of genotypes that exhibit high level of intra-season and inter-season stability for enhanced expression of LR, SG, and Pbs will minimize the impact of CGM on FSRY and SRDM% that results from seasonal effects. Such genotypes should also provide habitat for and thus help to ensure the survival of T. aripo in cassava fields. The study has contributed to the promotion of food security through identification of early-bulking genotypes which also have good potential for extended underground storability of roots. Early-bulking, high FSRY and SRDM% and SRR resistance are farmer-preferred traits. Therefore, enhancement of such traits through plant breeding is likely to increase the adoption of new genotypes by farmers. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.
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Participatory-based development of early bulking cassava varieties for the semi-arid areas of Eastern Kenya.Kamau, Joseph Wainaina. January 2006 (has links)
Cassava (Manihot esculenta Crantz) is an important food security crop in the semi-arid
areas of Eastern Kenya. It provides food for more days in a calendar year than any other
crop grown. Kenya has relied on varieties bred in other countries and because of this,
local breeding methodologies and expertise are lacking. Access to appropriate varieties
and adequate planting materials are major limiting factors to cassava production.
Farmers grow late bulking landraces that take up to 18 mo to harvest. Efforts to introduce
early bulking genotypes from IITA failed because of poor end-use quality. Local cassava
breeding is necessary to alleviate the production constraints. Before a local breeding
program can be established, farmers' preferences and production constraints must be
identified and methodology appropriate to the Kenyan environment must be developed.
The aims of this study were to identify farmer production constraints and preferences, to
develop methods appropriate for cassava breeding in the semi-arid areas of Kenya,
develop a population segregating for bulking period to estimate genetic variances that
would explain the gene effects controlling yield components, and through participatory
selection identify varieties that combine early bulking and preferred end-user traits.
PRA tools, focus groups and individual interviews were used to identify production
constraints and farmer preferences for cassava varieties. The PRA found that farmers
grow 13 landraces in the area and 11 production constraints were identified and
prioritised. The four most limiting in the order of importance were drought, lack of
planting material, pests and diseases.
Crosses between cassava varieties often do not produce much seed and the seed
produced does not germinate well. Germination studies were done with open pollinated
seeds to identify conditions favourable for seed germination in Kenya. The highest
germination of the seeds was at 36°C. The control seeds had a higher germination
percent (77%) compared to the seeds which were pre-heated at 36°C (57%).
Crosses were made between selected IITA and local Kenyan genotypes following the
NC 11 mating design to develop new genotypes which combine early bulking along with
other farmer/end-user preferred characteristics. The hybrid progenies were evaluated in
a seedling trial and clone genotypes advanced to a clonal trial and performance trial. The
clonal trial was destroyed by red spider mites and cassava green mites, and only the tolerant 225 genotypes were planted in a performance trial that was harvested at 6, 7
and 8 mo after planting. The SCA effects were estimated to be 57% to 75% for most of
the traits, except root number, which was mainly controlled by GCA effects (55%).
Participatory selection of genotypes that combined early bulking and end-user qualities
at the 7 and 8 mo after planting was done by farmers. Thirty genotypes that combined
early bulking and end-user qualities were identified and ranked according to their
performance in both agronomic and end-use traits using a selection index. A number of
selected genotypes yielded more than three times the yield of the best parents, showing
strong progress in breeding. Combining the farmers' preference aggregate score and the
selection index based on the agronomic data, assisted in the final identification of the
best genotypes developed in the breeding process. These results clearly demonstrated
that it is possible to breed early bulking varieties with good end-use quality in the semiarid
areas. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.
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Breeding, evaluation and selection of Cassava for high starch content and yield in Tanzania.Mtunda, Kiddo J. January 2009 (has links)
High starch content is an important component of root quantity and quality for almost all uses of cassava (flour, chips, and industrial raw material). However, there is scanty information on genetic variability for dry matter and starch contents and relatively little attention has been paid to genetic improvement of root dry matter content and starch content in Tanzania. The major objective of this research was to develop improved cassava varieties that are high yielding, with high dry matter and starch content for Tanzania and specifically to: i) identify farmers’ preferences and selection criteria for cassava storage root quality characteristics and other traits of agronomic relevance for research intervention through a participatory rural appraisal; ii) determine the genotypic variability for starch quantity and dry matter content evaluated for three harvesting times in four sites; iii) determine the inheritance of dry matter and starch content in cassava genotypes; and iv) develop and evaluate clones for high storage root yield, high dry matter content and starch. Attributes desired by farmers were yield, earliness, tolerance to pests and diseases. The complementing attributes associated with culinary qualities were sweetness, good cookability, high dry matter content or mealyness and marketability. The preliminary study conducted to evaluate the variability in root dry matter content (RDMC) and starch quantity and yield of ten cassava cultivars indicated that RDMC ranged from 29 to 40% with the mean of 34.3%. The RDMC at 7 months after planting (MAP) was higher than at 11 and 14 MAP. Starch content (StC) ranged from 20.3% to 24.9% with the mean of 22.8%. The StC differed significantly between cultivars, harvesting time and sites. An increase in StC was observed between 0 and 7 MAP, followed by a decline between 7 and 11 MAP, and finally an increase again noted between 11 and 14 MAP. However, for most of the cultivars at Kibaha an increase in StC between 11 and 14 MAP could not surpass values recorded at 7 MAP. At Kizimbani, cultivar Kalolo and Vumbi could not increase in StC after 11 MAP. At Chambezi and Hombolo, a dramatic gain in StC was observed for most of the cultivars between 11 and 14 MAP. Starch yield ranged from 0.54 to 4.09 t ha-1. Both StC and fresh storage root yield are important traits when selecting for commercial cultivars for starch production. Generation of the F1 population was done using a 10 x 10 half diallel design, followed by evaluation of genotypes using a 4 x 10 á-lattice. Results from the diallel analysis indicated that significant differences in fresh storage root yield (FSRY), fresh biomass (FBM), storage root number (SRN), RDMC, starch content (StC), and starch yield (StY), and cassava brown streak disease root necrosis (CBSRN) were observed between families and progeny. The FSRY for the families ranged from 15.0 to 36.3 t ha-1; StC ranged from 23.0 to 29.9%; RDMC ranged from 31.4 to 40.1%; and StY ranged from 3.3 to 8.3 t ha-1. The cassava mosaic disease (CMD) severity ranged from 1.7 to 2.7, while cassava brown streak disease (CBSD) severity for above ground symptoms ranged from 1.0 to 1.9. Additive genetic effects were predominant over non-additive genetic effects for RDMC, StC, and CBSRN, while for FSRY, FBM, SRN, and StY non-additive genetic effects predominated. Negative and non-significant correlation between RDMC and FSRY was observed at the seedling stage (r=-0.018), while at clonal stage the correlation was positive but not significant (0.01). The RDMC and StC were positive and significantly correlated (r=0.55***) at clonal stage. However, the StC negatively and non-significantly correlated with FSRY (r=- 0.01). High, positive and significant correlation (r=0.94; p.0.001) was observed between the StY and FSRY at clonal stage. High, positive and significant correlations between the seedling and clonal stage in FSRM (r=0.50; p.0.01), RDMC (r=0.67; p.0.001), HI (r=0.69; p.0.001), and SRN (r=0.52; p.0.01) were observed, suggesting that indirect selection could start at seedling stage for FSRM, RDMC, HI, and SRN. The best overall genotype for StC was 6256 (40.9%) from family Kiroba x Namikonga followed by genotype 6731 (40.6%; Vumbi x Namikonga). Among the parents, Kiroba and Namikonga were identified as the best combiners in terms of GCA effects for StC. Genotype 6879 from family Vumbi x AR 42-3 had the highest StY value of 34.8 t ha-1 followed by genotype 6086 (30.4 t ha-1; Kalolo x AR 40-6). Among the parents, Kalolo and AR 42-3 were identified as good combiners for the trait. Mid-parent heterosis for StC ranged from 41.6 to 134.1%, while best parent heterosis ranged from 30.4 to 119.6%. Genotype KBH/08/6807 from family Vumbi x TMS 30001 had the highest mid-and best parent heterosis percentage for StC. For StY, mid-parent and best parent heterosis ranged from 168.0 to 1391.0%, and from 140.4 to 1079.0%, respectively, with the genotype 6879 (Vumbi x AR 42-3) exhibiting the highest mid- and best parent heterosis percentage for StY. Improvement for StC, RDMC, and CBSRN may be realized by selecting parents with the highest GCA effects for the traits and hybridize with those that combine well to maximize the positive SCA effects for the StC, RDMC and CBSRN. Selected genotypes from the clonal stage will be evaluated in preliminary yield trial and advanced further to multi-locational trials while implementing participatory approaches involving farmers and processors in selection. New promising lines should be tested at different sites and the best harvesting dates should be established. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.
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