Thesis--Auburn University, 2009. / Abstract. Vita. Includes bibliographical references (p. 44-48).

Assignment (MPhil)--University of Stellenbosch, 2005. / Bibliography. Also available via the Internet.

The genus Loricaria Linnaeus of the family Loricariidae is diagnosed as a monophyletic group based on two uniquely derived synapomorphies: lower lip surfaces covered with filiform papillae and absence of buccal papillae surrounding the dentary teeth. Loricaria are small to moderate-sized catfishes widely distributed throughout most of the major river drainages of cis-Andean South America, including the Amazon, Orinoco, Paraguay, Paraná, and smaller coastal rivers draining the Guyana and Brazilian Shields. Based on multivariate analyses of morphometric and meristic data in combination with qualitative assessment of external morphological characters, 15 nominal species are accepted as valid, with an additional nine described as new. Among the nominal species, L. lentiginosa is synonymized with L. prolixa. Loricaria carinata is resurrected from synonymy with L. cataphracta and L. simillima is treated as a junior synonym of L. carinata. Formal diagnoses, descriptions, and a key to all 24 species are provided. Phylogenetic analyses of Loricaria and related taxa (Spatuloricaria, Crossoloricaria, Planiloricaria, Brochiloricaria, Paraloricaria, and Ricola) based on a combined data set of 32 gap-coded quantitative (morphometric and meristic) characters and 24 qualitative characters derived from external morphology resulted in fully resolved, but poorly supported phylogenies under equal weights and implied weighting criteria. Implied weights analyses using a concavity constant (K) of 24-49 recovered Loricaria as a monophyletic group with L. piracicabae as basal and sister to six terminal clades containing all other species. Species originally described as Loricaria, but subsequently transferred to Brochiloricaria, Paraloricaria, and Ricola, were recovered as paraphyletic in each analysis and should be treated as congeneric with Loricaria. Distributions of Loricaria exhibit patterns of endemism similar to those documented for other groups of Neotropical fishes. Species diversity is highest in the Amazon basin (13 species, including 10 endemics), followed by the Paraguay region (5 species, including 4 endemics), Guianas region (5 species, including 2 endemics), Orinoco basin (3 species, including 2 endemics), and a single species occurring in both the São Francisco basin and drainages of Northeastern Brazil. Loricaria is apparently absent from coastal Atlantic drainages south of the São Francisco basin. Such shared patterns of endemism provide some insight into past geomorphological processes that have influenced diversification in Loricaria and serves as a foundation for further tests of biogeographic hypotheses based on phylogenetic and geological evidence.

Two forms of gonadotropin-releasing hormone (GnRH) were extracted from brain-pituitary tissues of two species of Thai catfish, Clarias inacrocephalus and C. batrachus. The peptides were detected using high-performance liquid chromatography (HPLC) and radioimmunoassay (RIA), The amino acid sequences of both forms were determined using Edman degradation. One form of GnRH in the brain-pituitary tissues of the Thai catfish was novel, whereas the second form of GnRH was identical to chicken GnRH-XI. The presence of the N-terminal pGlu residue in both peptides was established by digestion with pyroglutamyl aminopeptidase. In addition, catfish GnRH-I was studied by mass spectrometry. The localization of these two peptides was determined to be in the discrete brain areas and in the pituitary of female and male catfish, C. macrocaphalus, using heterologous and homologous radioimmunoassays. Initially a heterologous RIA was used with mammalian GnRH as iodinated tracer and standard, and an antiserum made against salmon GnRH. Catfish GnRH-I (novel form) was found in most areas of the female and male brain with the highest content and concentration in the female pituitary and in the male hypothalamus,, Catfish GnRH-II (chicken GnRH-II) was found with the highest content in the female Cerebellum and highest concentration in the pituitary, catfish GnRH-II (chicken GnRH-II) was found with the highest content and concentration for males in the same area, hypothalamus. Additionally, a homologous RIA was used with catfish GnRH-II (chicken GnRH-II) as iodinated tracer and standard, and an antiserum prepared against chicken GnRH-II. Catfish GnRH-II was detected with the highest content and concentration in the cerebellum of both sexes. These values are higher than the results obtained in the heterologous assay. The location of catfish GnRH-I suggests that it plays a role in regulating the release of gonadotropin from the pituitary since the high content and concentration of this immunoreactive GnRH are detected in the hypothalamus and pituitary gland. In contrast, catfish GnRH-II may act as a neurotransmitter in the catfish brain, in particular in tha cerebellum where a high content and concentration of immunoreactive GnRH are detected. Physiological in vivo studies indicate that catfish GnRH-II is more effective than catfish GnRH-I and other forms of GnRH such as mammalian and dogfish GnRH for induction of ovulation in catfish, C. macrocephalus. Eight GnRH analogs had varying potencies for the induction of ovulation, but the most effective forms were two forms of catfish GnRH-II (chicken GnRH-II) modified in positions six and ten. In vitro studies found that catfish GnRH-I not only causes the release of gonadotropin but also the release of growth hormone in a dose-dependent manner. The primary structures of the two catfish GnRH peptides are important for understanding the evolution of this family peptide. The novel catfish GnRH shows that only positions 5, 7 and 8 vary in the GnRH molecule in jawed vertebrates, whereas catfish GnRH-II provides direct evidence that the structure of this GnRH is conserved in teleosts. / Graduate

No description available.

Inadequate seed production has historically been a factor limiting the commercial culture of Clarias gariepinus. The need to determine the environmental requirements of larvae was identified, in order to facilitate their successful mass rearing in hatcheries. The effects of key environmental factors on the growth, survival and aspects of the behaviour of C. gariepinus larvae and juveniles were investigated under controlled conditions. A strong emphasis was placed on the interpretation of the observed responses in terms of the natural history of the animal. Larvae were obtained by artificially inducing and spawning feral adult broodfish. Larval growth rates were highest in the temperature range 26-33ºC, with a peak at 30ºC. Survival of larvae was high between 22 and 33ºC. The final temperature preferendum of juveniles, determined in a thermal gradient was 30ºC, and it was concluded that this temperature is probably optimal for most of the physiological processes of the animal. Larvae and juveniles displayed strong negative phototaxis. When reared under different photoperiod regimes, larval growth increased with longer dark periods, however a similar rate of mortality was recorded in all photoperiod treatments. The provision of cover was found to enhance larval growth under conditions of continual light (24L/OD), however under conditions of continual darkness (OL/24D) similar growth rates were recorded regardless of the presence or absence of cover. All growth rates in continual darkness (OL/24D) were higher than those in continual light (24L/OD), regardless of whether cover was provided or not. It was concluded that a OL/24D photoperiod is optimal for larval rearing. At salinities between 0 and 5% similar growth and mortality rates of larvae were recorded. At 7.5 % larval growth and survival rates were lower, and at 10 % all larvae died within 48 hours. The 96h-LCSO for unionised ammonia was found to be 2.3 mg/l. The cytological effects of unionised ammonia were evident as degenerative changes in the gill and liver tissues. In an investigation of tank hygiene, it was found that larval growth and survival rates were highest in tanks not cleaned at all. As the frequency of tank cleaning increased, the growth and survival rates of larvae decreased. When reared at densities ranging from 45-450/1, larval growth was found to be density dependent. A model of hatchery productivity (number larvae of standard size produced/time/vol.) was developed based on the density dependent nature of larval growth. The model predicts that maximum productivity, will be achieved at a rearing density of 1400/1. The high growth and survival rates obtained over a broad range of each environmental parameter investigated serve to explain how larvae survive and grow in their unstable floodplain habitat in nature. The broad environmental tolerances of C. gariepinus are of benefit with respect to the culture of larvae, as successful larval rearing is possible over a fairly wide range of environmental conditions.

Distribution of the orangefin madtom (Noturus gilberti) was determined from 347 sites sampled in Virginia and North Carolina. This species inhabited 264 stream kilometers, over twice the reported range, in the following systems: Craig Creek, Roanoke River, Dan River, Big Chestnut Creek, South Mayo River, Pigg River, and Smith River. The orangefin madtom was somewhat common; 33% (Dan River) to 70% (Craig Creek) of the sites sampled were occupied. Negative interspecific associates of orangefin madtoms included chubs, mountain redbelly dace, rosyside dace, crescent shiners, and crayfish; only Roanoke darters were considered positive associates. Sand and silt levels were significantly lower at sites with N. gilberti, while percentage of small cobble, local gradient, and depth were significantly higher. Discriminant function analysis identified large gravel, local gradient, silt, and occurrence of rosyside dace and crayfish, as significant predictors of the occurrence of the orangefin madtom. Seasonal samples from Craig Creek consisted of three age groups. The smallest individual captured was 33 mm total length (TL) and the largest was 111 mm TL. Mortality appeared moderate from age I until summer of the third year, when most individuals apparently died shortly after spawning. Spawning habitat of orangefin madtoms in Craig Creek appeared to be fast-water riffles dominated by small cobble substrate. Attempts to induce N. gilberti spawning in the laboratory were unsuccessful due to high mortality of captive fish. Field-collected margined madtom egg masses, however, were successfully hatched, and subsequent survival was significantly greater for fry fed ground trout chow versus live brine shrimp nauplii. / Master of Science

Three hundred rainbow trout from each of two strains (Wytheville and the Soap Lake) were exposed in equal numbers to three non-overlapping wavebands of light (red, green, blue). One hundred fish from each strain were used as a control. These fish were exposed to unfiltered light of intensity equal to that of the three spectral treatments (21.4 lux). All fish were exposed to continuous light of the assigned experimental spectra for 53 days. Fish were fed trout chow twice daily at a rate previously found to maximize growth and efficiency. This feeding rate was determined by consulting hatchery records and the feeding charts developed by Haskell (1959). Mean length and mean weight of test animals may be affected by the rearing of individuals under specific wavebands of light (α = .001). Fish grown under blue or green light had the greatest mean length and fish grown under control or green light had the greatest mean weight while those reared under red light had the lowest mean length and mean weight. Although differences in coefficient of condition, yield, ferd conversion ratio, and survival were not Significant between light treatments (α = 0.05) in my study, significant differences may possibly be found in studies with a larger sample size. Although high variability and small sample size limit interpretation, this study suggests that certain light treatments used in conjunction with certain strains of fish could improve hatchery production. / Master of Science

Typescript (Photocopy) / Includes 3 published papers by the author in back of volume 2 / Bibliography: leaves 510-541 of vol. 1 / 2 v. : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--Dept. of Zoology, University of Adelaide, 1990

This thesis provides estimates of the market potential for farm-reared channel catfish in Virginia and Washington, D. C., the production costs and estimated returns of two small catfish farm enterprises, the present fisheries industry structure in Virginia, and the present channel catfish products and their feasibility for Virginia. The market potential for farm-reared channel catfish for Washington, D.C. and Virginia combined is estimated to be around 4,244,000 pounds (dressed weight) annually. The net returns generated by the two fish farm enterprises studied ranged from -$63.49/1.5 acre to $189.53/1.5 acre and the rates of return ranged from -8.9% to 27.4% at a $.30 per pound (live weight) price level. The present fish industry in Virginia is composed of a large number of wholesalers of various sizes who supply most of the retailers in the state. Most of the small wholesalers would not be receptive toward catfish products. However, some of the larger wholesalers may be willing to deal in catfish. In conclusion the food fish market seems to be the most feasible for absorbing farm-record channel catfish products and for providing an opportunity for an expanding farm-reared channel catfish industry in Virginia. / M.S.