Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-13T21:42:04Z No. of bitstreams: 1 2017 - Andr? William Masseaux Vidal J?nior.pdf: 1275550 bytes, checksum: d2d211215832b8ab2e53ab8d6da5ae4b (MD5) / Made available in DSpace on 2018-09-13T21:42:04Z (GMT). No. of bitstreams: 1 2017 - Andr? William Masseaux Vidal J?nior.pdf: 1275550 bytes, checksum: d2d211215832b8ab2e53ab8d6da5ae4b (MD5) Previous issue date: 2017-02-15 / It was proposed by this study to evaluate two protocols (PA and PB) to obtain autologous canine PRP, which is easy to perform in an ambulatory (semiautomatic method) and of good quality (platelet concentration, qualitative evaluation of platelet morphology and low contamination with Erythrocytes), to later propose different therapeutic indications of these PRPs as tissue modulating agents, according to the observed cellular leukocyte pattern. For this purpose, 20 dogs (Canis lupus familiaris) were used at the UFRRJ (HV) Veterinary Hospital, males and females, aged between 1 and 7 years (mean 4 years), considered clinically and hematologically healthy for elective surgeries and / or routine consultations. After adequate trichotomy and antisepsis, 8 mL of blood were collected by venipuncture of the jugular, being immediately packed in two 4 mL flasks, vacuntainer type, containing sodium citrate 3,2%. Protocol A using double centrifugation with 210 xG and 370 xG and protocol B using double centrifugation with 140 x G and 330 x G. PRP samples obtained from each protocol were used to count platelets, erythrocytes and leukocytes in the Neubauer chamber, differential leukocyte counting and observation of platelet morphology in smears. Data (mean and standard deviations) were analyzed by the 95% probability t test (p <0,05) using Pearson's correlation to test the relationship between platelet and erythrocyte counts, platelets and leukocytes and leukocytes in Relation to red blood cells. There was a very weak negative correlation between platelets and leukocytes (? = -0,03), weak negative between platelets and erythrocytes (? = -0,3) and strong positive correlation between leukocytes and erythrocytes (? = 0,75). Although Protocol B did not reach the desired one million platelets average (979300 ? 79631 cells / ?L), both protocols, A and B (4,42 ? 1,61 and 3,85 ? 1,55 times more platelets than Total blood, respectively) (p <0,05) were efficient in concentrating platelets. The cytoplasmic prolongations evidencing platelet activation were present in 26,55 ? 6,72% of platelets of protocol A and 26,25 ? 7,03% in those of protocol B (p> 0,05). A and B presented a small number of red blood cells (p> 0,05), which were considered to be contaminants of the samples and, for the quantity of leukocytes, protocol A presented more white blood cells (p <0,05) than protocol B with higher concentrations of basophils , and lymphocytes. / Foi proposto por esse estudo avaliar dois protocolos (PA e PB) para obten??o de PRP canino aut?logo, de f?cil execu??o em ambulat?rio (m?todo semiautom?tico) e de boa qualidade (capacidade de concentra??o de plaquetas, avalia??o qualitativa da morfologia das plaquetas e reduzida contamina??o com eritr?citos), para posteriormente propor diferentes indica??es terap?uticas desses PRP como agentes moduladores de recupera??o tecidual, de acordo com o padr?o celular leucocit?rio observado. Para isso foram utilizados 20 c?es (Canis lupus familiaris) atendidos no Hospital Veterin?rio da UFRRJ (HV), machos e f?meas, com idade variando entre 1 e 7 anos (m?dia 4 anos), considerados saud?veis clinica e hematologicamente que se destinavam para cirurgias eletivas e/ou consultas de rotina. Ap?s tricotomia e antissepsia adequadas, eram coletados 8 mL de sangue por venopun??o da jugular sendo imediatamente acondicionados em dois frascos de 4 mL, do tipo vacuntainer, contendo citrato de s?dio a 3,2%. O protocolo A utilizando centrifuga??o dupla com 210 xG e 370 xG e protocolo B utilizando centrifuga??o dupla com 140 xG e 330 xG. Amostras de PRP obtidas a partir de cada protocolo foram destinadas a contagem de plaquetas, hem?cias e leuc?citos em c?mara de Neubauer, contagem diferencial dos leuc?citos e observa??o da morfologia das plaquetas em esfrega?os. Analisou-se os dados (m?dias e desvios padr?o) pelo Teste t com 95% de probabilidade (p<0,05) utilizando-se correla??o de Pearson para testar a rela??o entre a contagem de plaquetas e hem?cias, plaquetas e leuc?citos e leuc?citos em rela??o as hem?cias. Houve correla??o negativa muito fraca entre plaquetas e leuc?citos (?= -0,03), negativa fraca entre plaquetas e hem?cias (?= -0,3) e correla??o positiva forte entre leuc?citos e hem?cias (?=0,75). Embora o protocolo B n?o tenha alcan?ando a m?dia um milh?o de plaquetas desejado (979300 ? 79631 c?lulas/?L), ambos os protocolos, A e B (4,42 ? 1,61 e 3,85 ? 1,55 vezes mais plaquetas que o sangue total, respectivamente) (p<0,05) foram eficientes em concentrar plaquetas. Os prolongamentos citoplasm?ticos evidenciando ativa??o plaquet?ria estiveram presentes em 26,55 ? 6,72 % das plaquetas do protocolo A e 26,25 ? 7,03 % nas do protocolo B (p>0,05). PA e PB apresentaram reduzido n?mero de hem?cias (p>0,05) consideradas contaminantes das amostras e, quanto a quantidade de leuc?citos, o protocolo A apresentou mais gl?bulos brancos (p<0,05) que o protocolo B com maiores concentra??es de bas?filos, segmentados e linf?citos.