Purpose: Retinal ganglion cell (RGC) loss is a measure of the progression of many visual disorders. It is important to identify RGCs with good specificity, so RGC numbers can be reliably analyzed. The purpose of this study was to analyze the effectiveness of two current RGC markers: Brn3a immunohistochemistry and the expression of Thy1-CFP in the Thy1-CFP transgenic mouse. Methods: Rhodamine-?-isothiocyanate (RITC) retrograde labeling, immunohistochemistry, wholemount retinal imaging, western blot, cross sectional analysis and cell densities in uninjured control animals and 3, 5, 7 and 14 days post-optic nerve crush (ONC) or transection (ONT) were tabulated. Results: Brn3a positive (Brn3a+) cell density was significantly less than RITC positive (RITC+) cell density in control mice. After ON injury, Brn3a+ cell density did not decrease at the same rate as RITC+ cell density. The density of RGCs that express Brn3a was significantly less than the individual Brn3a+ and RITC+ cell density at all experimental time points. Thy1-CFP positive (Thy1-CFP+) cell density was significantly less than RITC+ in control mice and significantly more than RITC+ cell density 14 days after ON injury. Thy1-CFP co-localized with ChAT positive (ChAT+) cells 7 days after ONT. Conclusion: Brn3a and Thy1-CFP are not reliable markers of RGCs. Retrograde labeling remains one of the most reliable methods of labeling RGCs in mice.

With corporate social responsibility (CSR) becoming more important to stakeholders and thus firms, understanding the relationship between CSR and corporate financial performance (CFP) is becoming more and more important. Although there is much research examining the general CSR-CFP relationship, there is very little, if any, research that investigates the CSR-CFP association across industries. With a sample of 429 firms from the S&P 500, my study looks to see if this association differs between the consumer and nonconsumer sectors. Time-series regression analyses reveal that while the CSR-sales relationship is negative for both consumer and nonconsumer companies, the CSR-gross profit association is more positive for nonconsumer than consumer firms.

Vibrio harveyi is a bioluminescent bacterium and the organism in which quorum sensing was discovered. It was recently found that a class of molecules, cyclic dipeptides, may be a new kind of quorum sensing signal that may affect other species in the genus. The purpose of this study was to determine if V. harveyi produced one of these molecules, cyclo(Phe-Pro) or cFP, and the effects it has on bioluminescence, growth and gene expression. Electrospray Mass Spectrometry was used to detect cFP, and it was found. While growth and gene expression were not significantly affected by cFP, bioluminescence was slightly induced at low concentrations. It appears that V. harveyi does not produce cFP and it does not significantly affect the luminescence quorum sensing controlled genes, and is most likely not a true signal, in V. harveyi.

Over the past decade, sustainability has emerged as one of the foremost issues faced by corporations across all sectors and Corporate Social Responsibility has gained much momentum in the past two decades. This research investigated whether investors in emerging markets are equally concerned about a firm’s social and environmental impacts as their counterparts in developed economies. The aim was to ascertain whether or not a correlation exists between CSR and stock market performance of South African listed companies. This was the first study undertaken in South Africa that specifically investigated the relative performances of SRI listed and non-SRI listed companies. The findings reveal that there are observable differences between the average market returns of the FTSE/JSE Socially Responsible Investment Index and the FTSE/JSE All Share Index, as well as the average price/earnings ratios and average price/book value ratios of all companies listed the JSE Main Board. Although two out of the three hypotheses failed to yield significant statistical outcomes, all the findings were in favour of the SRI. The research has opened up the avenue for future studies to investigate the purported links between sustainability and financial performance in the context of emerging markets. Copyright / Dissertation (MBA)--University of Pretoria, 2010. / Gordon Institute of Business Science (GIBS) / unrestricted

Corporate Social Responsibility (CSR) is a widely disputed and constantly evolving topic. One of the most recent methods of assessing CSR in South Africa has been through the usage of the Financial Times Stock Exchange/Johannesburg Stock Exchange (FTSE/JSE) Responsible Investment Index. The Johannesburg Stock Exchange (JSE) Socially Responsible Investment Index (SRI Index) was founded in 2004 and was replaced by the FTSE/JSE Responsible Investment Index in 2015. The index evaluates listed firms based on their triple bottom line performance i.e., environment, society, and economy. The index includes companies that are thought to have good CSR policies. This study assesses the effects of CSR on the Corporate Financial Performance (CFP) of firms listed on the JSE that were included in the FTSE/JSE All Share Index (ALSI) as of the 31st of January 2021. This it does by analyzing the stock's financial performance over a five-year period between the 2015 and 2019 financial years. The requirements for stocks to be included in this study is that they must have had an average market capitalization between R2 billion and R100 billion between the 2015 and 2019 financial years and are not part of the real estate sector. The results of the nonparametric (Mann-Whitney) tests show that companies that are part of the FTSE/JSE Responsible Investment Index perform better on average than those that are not included in the index. This conclusion is based on a review of the total return index (TRI), return on equity ratio (ROE), price-earnings ratio (PE), and the market-to-book ratio (MB). The analysis conducted using the net profit margin (NPM) as a measure of financial performance show that there is no relationship between CSR and financial performance. The Mann-Whitney test results where the return on assets (ROA) ratio was used showed a negative relationship between CSR and financial performance.

Bio-oil generated from biomass is becoming one of the most promising alternatives as potential energy sources to replace fossil fuels in the transportation sector. Fast pyrolysis of biomass is one of the most economically feasible ways to produce bio-oil according to recent research on thermochemical conversion of biomass. Upgrading of oils derived from to hydrocarbon fuels requires oxygen removal and molecular weight reduction.  Catalytic cracking and hydrotreating are two efficient processes to upgrade bio-oil. Hydrotreating requires that hydrogen is added in the process to increase the H/C ratio of the product. Normally, catalytic fast pyrolysis and hydrotreating are two separated processes. In order to increase the energy efficiency of the process, exploring the fast pyrolysis of biomass with in-situ catalyst under the hydrogen atmosphere, i.e. catalytic hydropyrolysis shall be very interesting, and this is the objective of this work. In this work, biomass pyrolysis experiments using softwood have been performed in hydrogen and nitrogen atmospheres with/without catalyst. It was found that in the case of the H2 atmosphere, a higher yield on oil phase and a reduced water production is found. More oxygen was removed as CO and CO2. The catalytic fast pyrolysis (CFP) under H2 atmosphere also produce relatively more PAH (polymer aromatic hydrocarbon) and less MAH (monomer aromatic hydrocarbon) than under N2 atmosphere.

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Given that Sweden is one of the most sustainable countries in the world (RobecoSAM, 2018), with a big number of its companies as active participants in CSR, we investigate the impact that these CSR activities could have on CFP with a focus on the Financial sector, using in 26 listed Financial Institutions. As we will find out from the existing literature, the CSR-CFP relationship is neither strictly negative nor positive. If it is positive, then the firm will allocate more resources to CSR to achieve better financial performance, and the firm may fore-go or approach CSR initiatives with caution if they affect CFP negatively. Past researchers have studied this relationship before and found that the reason why financial institutions get involved in socially responsible activities is to gain public trust and justification usually after a public financial scandal. So, we explore this further expecting that if it is indeed a matter of fact that these institutions gain the justification from the public that they so desire when they choose to get involved in socially responsible activities, then it should be that the public trust and justification would translate into improved financial performance. Therefore, the research question that we seek to answer is “Does Corporate Social Responsibility (CSR) have an Impact on Corporate Financial Performance (CFP) in the Listed Swedish Financial Institutions” We examined the relationship between CSR and CFP using 26 Swedish financial institutions that are listed on Nasdaq Nordic stock exchange market (Stockholm) for the period between 2015 and 2019. The Fixed Effects Model regression analysis for panel data was used to test this relationship and we found that when Swedish financial institutions get involved in CSR, their financial performance is neither worsened or improved because of the insignificant ESG coefficients that we found.  Swedish financial institutions' engagement in socially responsible activities does not guarantee an impact on their financial performance.

Under de senaste årtiondena har ansvarfullt företagande (CSR) varit ett ämne som erhållit allt mer fokus inom forskning och således även för företag och samhället i stort. CSR är ett ämne som är högst aktuellt då globalisering är ett faktum och medför att de företag som är verksamma idag bär ett globalt ansvar, då dessa är stora aktörer i en värld som kantas av ekonomiska kriser, miljöproblem och humanitär försummelse. Det ökade intresset från samhället för CSR har medfört att företagen idag ser detta som en strategisk investering och företags satsning i hållbarhetsaktiviteter har kommit att erhålla en allt större del av företags verksamhet. Frågan kvarstår om detta endast är en investering som ökar företagets etik och moral eller om det faktiskt är lönsamt, och även om lönsamma företag ökar sin investering i hållbarhetsarbete. En stor mängd forskning har utförts för att undersöka förhållandena mellan hållbarhet och lönsamhet, dock utan att konsensus i forskningsgrenen har uppkommit.       Grundat på den nuvarande forskningssituationen syftade denna studie till att undersöka vilken effekt ansvarsfullt företagande har på olika lönsamhetsmått, samt vilken effekt de olika lönsamhetsmåtten har på ansvarsfullt företagande, i företag registrerade på large cap och mid cap på Nasdaq OMX Stockholm. För att besvara studiens problemformulering och uppfylla studiens syfte har en kvantitativ forskningsansats brukats och ett urval av företag listade på large och mid cap Nasdaq OMX Stockholm har undersökts. För att sätta ett mått på ansvarsfullt företagande (CSP) har en innehållsanalys av företags årsrapporter och hållbarhetsrapporter genomförts, lönsamhetsmåtten (CFP) har utgått från redovisningsbaserade mått (ROE och ROA) samt ett marknadsbaserat mått (total avkastning). Vidare undersöks även relationen mellan variablerna, CSP och CFP, med en tidsaspekt och kontrollvariablerna storlek, risk, FoU och industritillhörighet har brukats. De teorier som har använts för att förklara CFP:s effekt på CSP är slappa resurser och direktörsopportunism, och effekten CSP har på CFP har teorierna intressentteorin, resursbaserad synvinkel och kompromissteorin använts.  Det resultat som framkommit genom de multipla regressionerna som testat studiens hypoteser visar att ROE:s effekt på CSP är positiv, medan både ROA:s och avkastningens effekt på CSP är neutral. Slutsatsen blir således delad då lönsamhetsmåttet ROE visar att företag som har en högre lönsamhet kommer investera mer i hållbarhet, denna effekt knyts samman med teorin om slappa resurser. Å andra sidan visar både ROA och avkastning på en neutral effekt på CSP vilket ej kan knytas till varken slappa resurser eller direktöropportunism. Vidare när istället CSP:s effekt på ROE, ROA och avkastning undersökts visar samtliga på en neutral påverkan. Den slutsats som kan dras från detta resultat är att hållbarhetsarbete ej empiriskt kan säkerställa en högre eller lägre lönsamhet, och varken intressentteorin, resursbaserad synvinkel eller kompromissteorin kan förklara resultatet. Med icke-konklusiva resultat föreslår författarna för vidare forskning inom området där mer forskning i den svenska kontexten är nödvändig.

Les modifications post-traductionelles des histones contribuent à l’expression génique et à la stabilité du génome. La méthylation de la lysine 4 de l’histone H3 (H3K4me), une marque associée aux promoteurs de gènes transcrits, est déposé par les methyltransferases hautement conservées de la famille SET1, dans le contexte du complexe COMPASS. SET-2, l’homologue de SET1 chez Caenorhabditis elegans, est responsable de la déposition de H3K4me dans la lignée germinale, et son inactivation provoque une perte progressive de la fertilité. Le but de mon travail de thèse a été d’étudier comment SET-2 et la méthylation de H3K4 contribuent au maintien de la lignée germinale. J’ai montré que l’absence de SET-2 provoque une sensibilité accrue aux dommages à l’ADN. Cependant, les voies de signalisation et de réparation de ces dommages sont fonctionnelles dans le mutant set-2. Par séquençage de l’ADN, j’ai par ailleurs montré que la stérilité progressive observée en l’absence de set-2 n’est pas due à une capacité de réparation réduite. L’ensemble de mes résultats suggère que H3K4me pourrait agir en aval de la signalisation de dommages à l’ADN, en influençant l’organisation de la chromatine aux sites des cassures double brin. J’ai d’autre part mis en évidence une nouvelle fonction pour la méthylation de H3K4 dans l’organisation de la chromatine en montrant que set-2 interagit génétiquement avec le complexe Condensine II et la Topoisomérase II, facteurs clefs de l’organisation mitotique des chromosomes. Des expériences de microscopie par FLIM-FRET ont d’ailleurs validé une fonction de H3K4 méthylée dans l’organisation de la chromatine dans la lignée germinale. Enfin, j’ai montré par analyses transcriptomiques que la protéine CFP-1 du complexe COMPASS est impliquée dans la régulation du programme transcriptionnel de la lignée germinale et que cette fonction est indépendante de SET-2. L’ensemble de mes résultats montre comment la régulation chromatinienne impacte le maintien d’une lignée germinale fonctionnelle à plusieurs niveaux. / Post-translational modifications of histones contribute to gene expression and genome stability. Methylation of lysine 4 of histone H3 (H3K4me), a mark associated with actively transcribed genes, is deposited by the highly conserved SET1 family methyltransferases acting in COMPASS related complexes. SET-2, the SET1 homologue in Caenorhabditis elegans, is responsible for the deposition of H3K4me in the germ line, and its inactivation causes progressive loss of fertility. The purpose of my PhD work was to study how SET-2 and the methylation of H3K4 contribute to the maintenance of the germ line. I have shown that the absence of SET-2 causes increased sensitivity to DNA damage. However, the DNA damage-induced signaling and repair pathways are functional in the set-2 mutant. By DNA sequencing, I have also shown that the progressive sterility observed in the absence of set-2 is not due to a reduced repair capacity. Together, my results suggest that H3K4 methylation may act downstream of DNA damage signaling, potentially by influencing the organization of chromatin at the sites of double-strand breaks. I have also described a new function for H3K4 methylation in the organization of chromatin by showing that set-2 genetically interacts with the Condensitin II complex and Topoisomerase II, key factors in mitotic chromosome organization. Moreover, FLIM-FRET microscopy experiments have validated a role for H3K4 methylation in germline chromatin organization. Finally, using transcriptomic analyses, I have described a function for CFP-1, a component of the COMPASS complex, in the regulation of the germline transcriptional program independent of SET-2. Altogether, my results show how chromatin regulation affects the maintenance of a functional germline through multiple mechanisms.