MHRD PhD scholarship / With Multiprocessor Systems-on-Chips (MPSoCs) pervading our lives, security issues are emerging as a serious problem and attacks against these systems are becoming more critical and sophisticated. We have designed and implemented different hardware based solutions to ensure security of an MPSoC. Security assisting modules can be implemented at different abstraction levels of an MPSoC design. We propose solutions both at circuit level and system level of abstractions. At the VLSI circuit level abstraction, we consider the problem of presence of noise voltage in input signal coming from outside world. This noise voltage disturbs the normal circuit operation inside a chip causing false logic reception. If the disturbance is caused intentionally the security of a chip may be compromised causing glitch/transient attack. We propose an input receiver with hysteresis characteristic that can work at voltage levels between 0.9V and 5V. The circuit can protect the MPSoC from glitch/transient attack. At the system level, we propose solutions targeting Network-on-Chip (NoC) as the on-chip communication medium. We survey the possible attack scenarios on present-day MPSoCs and investigate a new attack scenario, i.e., router attack targeted toward NoC enabled MPSoC. We propose different monitoring-based countermeasures against routing table-based router attack in an MPSoC having multiple Trusted Execution Environments (TEEs). Software attacks, the most common type of attacks, mainly exploit vulnerabilities like buffer overflow. This is possible if proper access control to memory is absent in the system. We propose four hardware based mechanisms to implement Role Based Access Control (RBAC) model in NoC based MPSoC.

This thesis deals with design of hot water grate boiler, where the output is water with parameters 130 °C and pressure 16 bar. In the content of the whole thesis is gradually introduced a stoichiometric calculations, which is based on the specified fuel – wood chips. This is followed by the design of the individual heating surfaces according to the thermal calculations given in the thesis. Finally, the calculation is extended by hydraulic and aerodynamic losses. Dimensional design, as a basic scheme, is shown at thesis. More detailed drawing documentation is attached to this thesis.

The diploma thesis deals with the design of a grate boiler for the combustion of a mixture of RDF and wood chips with the specified output and parameters of superheated steam. The introduction consists of stoichiometric calculations and determination of the thermal efficiency of the boiler. The thesis is devoted to determining the dimensions of the boiler, heat transfer calculations, determining pressure losses, checking the heat balance and chlorine corrosion.

In this master thesis I have been dealt with the design and construction of an instrumental platform that used positioning focused laser beam (so-called optical tweezers) for manipulation with living cells without their damage.

This Master’s thesis deals with analysis of available identification chips and the design electronic attendance book that these chips will use and store the measured information to a file on a memory card. One of the tasks is to compare different kinds of identification cards and chips, and select the most appropriate type for the implementation process. Additionally, the work described the FAT file system, which is used for data storage card.

This project is devided to three parts - theoretical overview of cutting farm components, design of cutting farm for specified production cell and the design of chip conveyor for specified cuting machine. Theoretical overview introduces common commercial used components of cutting farm, their properties and using possibilities in particular regarding their applicability at cutting farm design and chip conveyor design. In the second part there is proposal of design of cutting farm for production cell consist of six identical multi-function machinig centers. For produced amount of chips there is proposal of their processing from the begining at cuting proccess up to their leavig production hall as a briquetts in a containers. At last part of this project there is complete design proposal of chip conveyor for mentioned multi-function maching center. Design is built from a basic line diagram up to design of particular components including calculations and analysis.

The thesis is dedicated to the design of the steam boiler which burns wood chips, with a natural circulation of the water. It is a grate boiler, which produces 20 t/h of the steam. The output parameters of the steam are 420 °C and 5 MPa. The feedwater temperature is 125 ° C .The thesis gradually deals with stoichiometric calculations and determines the efficiency of the boiler. Then it describes the design of the fireplace and thermal calculations of each heat transfer surfaces. The appendix includes drawings of the boiler.

Thesis (PhD (Chemical Engineering))--Cape Peninsula University of Technology, 2019 / The South African wine industry is constantly facing several challenges which affect the quality of wine, the local/global demand and consequently the revenue generated. These challenges include the ongoing drought, bush fires, climate change and several liquor amendment bills aimed at reducing alcohol consumption and alcohol outlets in South Africa. It is therefore critical for the wine industry to expand and find alternative ways in which sub-standard or surplus wine grapes can be used to prevent income losses and increase employment opportunities. Traditional Balsamic Vinegar (TBV) is a geographically and legislative protected product produced only in a small region in Italy. However, the methodology can be used to produce similar vinegars in other regions. Balsamic-styled vinegar (BSV), as defined in this thesis, is a vinegar produced by partially following the methods of TBV while applying process augmentation techniques. Balsamic-styled vinegar is proposed to be a suitable product of sub-standard quality or surplus wine grapes in South Africa. However, the production of BSV necessitates the use of cooked (high sugar) grape must which is a less favourable environment to the microorganisms used during fermentation. Factors that negatively affect the survival of the microorganisms include low water activity due to the cooking, high osmotic pressure and high acidity. To counteract these effects, methods to improve the survival of the non-Saccharomyces yeasts and acetic acid bacteria used are essential. The primary aim of this study was to investigate several BSV process augmentation techniques such as, aeration, agitation, cell immobilization, immobilized cell reusability and oxygen mass transfer kinetics in order to improve the performance of the microbial consortium used during BSV production. The work for this study was divided into four (4) phases. For all the phases a microbial consortium consisting of non-Saccharomyces yeasts (n=5) and acetic acid bacteria (n=5) was used. Inoculation of the yeast and bacteria occurred simultaneously. The 1st phase of the study entailed evaluating the effect of cells immobilized by gel entrapment in Ca-alginate beads alongside with free-floating cells (FFC) during the production of BSV. Two Ca-alginate bead sizes were tested i.e. small (4.5 mm) and large (8.5 mm) beads to evaluate the effects of surface area or bead size on the overall acetification rates. Ca-alginate beads and FFC fermentations were also evaluated under static and agitated (135 rpm) conditions. The 2nd phase of the study involved studying the cell adsorption technique for cell immobilization which was carried-out using corncobs (CC) and oak wood chips (OWC), while comparing to FFC fermentations. At this phase of the study, other vinegar bioreactor parameters such as agitation and aeration were studied in contrast to static fermentations. One agitation setting (135 rpm) and two aeration settings were tested i.e. high (0.3 vvm min−1) and low (0.15 vvm min−1) aeration conditions. Furthermore, to assess the variations in cell adsorption capabilities among individual yeast and AAB cells, the quantification of cells adsorbed on CC and OWC prior- and post-fermentation was conducted using the dry cell weight method. The 3rd phase of the study entailed evaluating the reusability abilities of all the matrices (small Ca-alginate beads, CC and OWC) for successive fermentations. The immobilized cells were evaluated for reusability on two cycles of fermentation under static conditions. Furthermore, the matrices used for cell immobilization were further analysed for structure integrity by scanning electron microscopy (SEM) before and after the 1st cycle of fermentations. The 3rd phase of the study also involved the sensorial (aroma and taste) evaluations of the BSV’s obtained from the 1st cycle of fermentation in order to understand the sensorial effects of the Ca-alginate beads, CC and OWC on the final BSV. The 4th phase of the study investigated oxygen mass transfer kinetics during non-aerated and aerated BSV fermentation. The dynamic method was used to generate several dissolved oxygen profiles at different stages of the fermentation. Consequently, the data obtained from the dynamic method was used to compute several oxygen mass transfer parameters, these include oxygen uptake rate ( 𝑟𝑟𝑂𝑂2 ), the stoichiometric coefficient of oxygen consumption vs acid yield (𝑌𝑌𝑂𝑂/𝐴𝐴), the oxygen transfer rate (𝑁𝑁𝑂𝑂2 ), and the volumetric mass transfer coefficients (𝐾𝐾𝐿𝐿𝑎𝑎). During all the phases of the study samples were extracted on weekly intervals to evaluate pH, sugar, salinity, alcohol and total acidity using several analytical instruments. The 4th phase of the study involved additional analytical tools, i.e. an oxygen µsensor to evaluate dissolved oxygen and the ‘Speedy breedy’ to measure the respiratory activity of the microbial consortium used during fermentation. The data obtained from the 1st phase of the study demonstrated that smaller Ca-alginate beads resulted in higher (4.0 g L-1 day−1) acetification rates compared to larger (3.0 g L-1 day−1) beads, while freely suspended cells resulted in the lowest (0.6 g L-1 day−1) acetification rates. The results showed that the surface area of the beads had a substantial impact on the acetification rates when gel entrapped cells were used for BSV fermentation. The 2nd phase results showed high acetification rates (2.7 g L-1 day−1) for cells immobilized on CC in contrast to cells immobilized on OWC and FFC, which resulted in similar and lower acetification rates. Agitated fermentations were unsuccessful for all the treatments (CC, OWC and FFC) studied. Agitation was therefore assumed to have promoted cell shear stress causing insufficient acetification during fermentations. Low aerated fermentations resulted in better acetification rates between 1.45–1.56 g L-1 day−1 for CC, OWC and FFC. At a higher aeration setting, only free-floating cells were able to complete fermentations with an acetification rate of 1.2 g L-1 day−1. Furthermore, the adsorption competence data showed successful adsorption on CC and OWC for both yeasts and AAB with variations in adsorption efficiencies, whereby OWC displayed a lower cell adsorption capability compared to CC. On the other hand, OWC were less efficient adsorbents due to their smooth surface, while the rough surface and porosity of CC led to improved adsorption and, therefore, enhanced acetification rates. The 3rd phase results showed a substantial decline in acetification rates on the 2nd cycle of fermentations when cells immobilized on CC and OWC were reused. While cells entrapped in Ca-alginate beads were able to complete the 2nd cycle of fermentations at reduced acetification rates compared to the 1st cycle of fermentations. The sensory results showed positive ratings for BSV’s produced using cells immobilized in Ca-alginate beads and CC. However, BSV’s produced using OWC treatments were neither ‘liked nor disliked’ by the judges. The SEM imaging results further showed a substantial loss of structural integrity for Ca-alginate beads after the 1st cycle fermentations, with minor changes in structural integrity of CC being observed after the 1st cycle fermentations. OWC displayed the same morphological structure before and after the 1st cycle fermentations which was attributed to their robustness. Although Ca-alginate beads showed a loss in structural integrity, it was still assumed that Ca-alginate beads provided better protection against the harsh environmental conditions in contrast to CC and OWC adsorbents due to the acetification rates obtained on both cycles. The 4th phase data obtained from the computations showed that non-aerated fermentations had a higher 𝑌𝑌𝑂𝑂/𝐴𝐴, 𝑟𝑟𝑂𝑂2 , 𝑁𝑁𝑂𝑂2 and a higher 𝐾𝐾𝐿𝐿𝑎𝑎 . It was clear that aerated fermentations had a lower aeration capacity due to an inappropriate aeration system design and an inappropriate fermentor. Consequently, aeration led to several detrimental biochemical changes in the fermentation medium thus affecting 𝐾𝐾𝐿𝐿𝑎𝑎 and several oxygen mass transfer parameters which serve as a driving force. Overall, it was concluded that the best method for BSV production is the use of cells entrapped in small alginate beads or cells adsorbed on CC under static and non-aerated fermentations. This conclusion was based on several factors such as cell affinity/cell protection, acetification rates, fermentation period and sensorial contributions. However, cells entrapped in Ca-alginate beads had the highest acetification rates. The oxygen mass transfer computations demonstrated a high 𝐾𝐾𝐿𝐿𝑎𝑎 when Ca-alginate beads were used under static-non-aerated conditions compared to fermentations treated with CC. Therefore, a fermentor with a high aeration capacity needs to be designed to best suit the two BSV production systems (Ca-alginate beads and CC). It is also crucial to develop methods which can increase the robustness of Ca-alginate beads in order to improve cell retention and reduce the loss of structural integrity for subsequent cycles of fermentation. Studies to define parameters used for upscaling the BSV production process for large scale productions are also crucial.

<div>Rare circulating cells are becoming a subject of interest due to their potential clinical applications to replace invasive procedures. Due their low presence in blood (as low as 1 in 1 ml of blood) various platforms are developed to capture and isolate them. Common limitations of current platforms include the inability to process large volumes of blood without an initial volume reduction step such as centrifugation, reliance on a single antibody for the capture, and the difficulty of releasing and retrieving the captured cells with high purity. A rare cell retrieval platform with high throughput operation and high purity retrieval is needed to capture these rare cells by processing large volumes of blood.</div><div><br></div><div>In this thesis study, we have developed a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the unwanted leukocytes. The second module is a microwell array that furthers the purification by magnetically guiding each cell into a separate well concurrently, and allows individual retrieval of each cell. Even though the system we have developed is applicable to many fields pertaining to rare cell capture, here we demonstrate the proof-of-concept using model cell lines that represent circulating fetal trophoblasts. We describe the design, operation as well as the experimental characterization of the system. Our characterization results show that the process can be completed within 145 minutes from the very beginning till the retrieval of a target cell, and can provide efficiencies and purities that are as high as 100%. </div><div><br></div><div>In order to demonstrate a real-world use case for our device, we present preliminary experiments done with blood samples from pregnant women. We show that we are able to retrieve candidate fetal cells under 167 minutes. Future work will be focused on sequencing the candidate fetal cells retrieved from maternal samples to confirm their fetal origin as well as enhancing system performance in maximizing the number of cells captured.</div><div><br></div>

Der Asynchronous Transfer Mode (ATM) stellt die zukünftige und einheitliche Basistechnologie für das Breitband-ISDN dar. Da nahezu alle wesentlichen Protokollfunktionen in Hardware realisierbar sind, soll nachfolgend ein Überblick über bereits angebotene VLSI-Schaltkreise gegeben werden. Eine Systematisierung und Einordnung vorhandener ATM-Chips hinsichtlich ihrer Leistungsfähigkeit und ihres Funktionsumfangs erfolgt in Hinblick auf das sogenannte B-ISDN-Referenzmodell. Dieses Schichtenmodell definiert die notwendigen Protokolle und Schnittstellen für den Asynchronous Transfer Mode. Zum grundlegenden Verständnis wird einleitend eine kurze Einführung in die Basisprinzipien von ATM gegeben.