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Beneficial effects of natural eggshell membrane versus placebo in exercise-induced joint pain, stiffness, and cartilage turnover in healthy, postmenopausal womenRuff, Kevin J, Morrison, Dennis, Duncan, Sarah A, Back, Matthew, Aydogan, Cem, Theodosakis, Jason 02 1900 (has links)
Purpose: Despite its many health benefits, moderate exercise can induce joint discomfort when done infrequently or too intensely even in individuals with healthy joints. This study was designed to evaluate whether NEM (R) (natural eggshell membrane) would reduce exercise-induced cartilage turnover or alleviate joint pain or stiffness, either directly following exercise or 12 hours post exercise, versus placebo. Patients and methods: Sixty healthy, postmenopausal women were randomly assigned to receive either oral NEM 500 mg (n=30) or placebo (n=30) once daily for two consecutive weeks while performing an exercise regimen (50-100 steps per leg) on alternating days. The primary endpoint was any statistically significant reduction in exercise-induced cartilage turnover via the biomarker C-terminal cross-linked telopeptide of type-II collagen (CTX-II) versus placebo, evaluated at 1 and 2 weeks of treatment. Secondary endpoints were any reductions in either exercise-induced joint pain or stiffness versus placebo, evaluated daily via participant questionnaire. The clinical assessment was performed on the per protocol population. Results: NEM produced a significant absolute treatment effect (TEabs) versus placebo for CTX-II after both 1 week (TEabs - 17.2%, P=0.002) and 2 weeks of exercise (TEabs - 9.9%, P=0.042). Immediate pain was not significantly different; however, rapid treatment responses were observed for immediate stiffness (Day 7) and recovery pain (Day 8) and recovery stiffness (Day 4). No serious adverse events occurred and the treatment was reported to be well tolerated by study participants. Conclusion: NEM rapidly improved recovery from exercise-induced joint pain (Day 8) and stiffness (Day 4) and reduced discomfort immediately following exercise (stiffness, Day 7). Moreover, a substantial chondroprotective effect was demonstrated via a decrease in the cartilage degradation biomarker CTX-II.
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Efeitos da administração oral de glucosamina e condroitim sulfato associados ao ácido hialurônico em cavalos com osteoartrite / Effects of oral administration of glucosamine sulfate and chondroitin sulfate associated with hyaluronic acid in horses with osteoarthritisCoelho, Joyce Martins 17 July 2009 (has links)
A osteoartrite é a causa mais comum de claudicação em cavalos e, frequentemente está associada com a queda de desempenho e abandono precoce das atividades esportivas. Numerosos estudos têm investigado o potencial da função dos condroprotetores na desaceleração do processo degenerativo e no reparo da cartilagem articular. A finalidade deste estudo foi investigar o efeito da administração oral de glucosamina, condroitim sulfato e ácido hialurônico associados sobre a evolução clínica, radiológica, e glicosaminoglicanos (GAGs) da urina, do líquido sinovial e sérico de cavalos acometidos por osteoartrite. Foram utilizados seis equinos, com idades entre seis e doze anos, machos ou fêmeas, submetidos ao mesmo manejo nutricional. Estes animais receberam doses diárias de condroitim sulfato (CS) (2,8 g), ácido hialurônico (AH) (0,1g), glucosamina (3,1 g), via oral, por 25 dias. Exames clínicos e radiográficos foram realizados anteriormente e após o tratamento. Amostras de urina, líquido sinovial, e sangue foram coletados antes da primeira administração do suplemento; a cada 5 dias durante o tratamento e a cada 7 dias após o tratamento, durante 55 dias. Os glicosaminoglicanos urinários foram identificados por eletroforese em gel de agarose após cromatografia de troca iônica e quantificados por densitometria. A determinação de AH sérico foi realizada por ELISA. Após proteólise do líquido sinovial o CS e a AH foram igualmente identificados por eletroforese e quantificados por densitometria. No início do experimento, as concentrações médias urinárias de CS, DS, HS e GAGs totais (2,96 mg/l, 0,66 mg/l, 0,42 mg/l, 4,05 mg/l respectivamente) foram inferiores as médias urinárias, logo após o tratamento (5,38 mg/l, 1,30 mg/l, 0,96 mg/l, 7,64 mg/l respectivamente) (p<0,05) e similares após 30 dias (4,24 mg/l, 1,09 mg/l, 0,36 mg/l, 5,69 mg/l). A concentração sérica de AH aumentou após o tratamento e ao final do experimento em relação do início dos mesmos (10,61 ng/ml, 12,58 ng/ml, 21,08 ng/ml respectivamente) (p<0,05). O CS do líquido sinovial apresentou comportamento similar ao CS urinário (início: 76,13 µg/ml, final do tratamento: 93,05 µg/ml, final do experimento: 61,61µg/ml). Já o AH no líquido sinovial não sofreu alterações significativas (início: 440,07 µg/ml, final do tratamento: 351,15 µg/ml, final do experimento: 375,99 µg/ml) apesar da tendência a diminuição. Concluiu-se que a administração oral de glucosamina, CS e AH associados apresenta uma tendência ao aumento dos GAGs urinários e do CS do líquido sinovial imediatamente após o tratamento, com diminuição até 30 dias; aumento sérico do AH e manutenção da concentração de AH no líquido sinovial, com tendência a diminuição. / Osteoarthritis is the most common cause of lameness in horses, and often is frequently associated with poor performance and early end of sports activities. Numerous studies have investigated the potential function of chondroprotective drugs in slow down the degenerative process and helping to repair the joint cartilages. The purpose of this study was to investigate the effect of oral administration of glucosamine, chondroitin sulfate and hyaluronic acid associated with the clinical, radiological, and urinary glycosaminoglycans (GAGs), in the serum and synovial fluid of horses affected by osteoarthritis. We used six horses, aged between six and twelve years old, male or female, undergoing the same nutritional management. These animals received daily doses of condroitim sulfate (CS) (2.8 g), hyaluronic acid (HA) (0.1 g), glucosamine (3.1 g), oral route, for 25 days. Clinical and radiographic examinations were performed before and at the end of treatment. Samples of urine, synovial fluid and serum were collected before the first administration of the supplement, and every 5 days during the treatment and every 7 days after the end of the treatment, for a total of 55 days. The urinary glycosaminoglycans have been identified for agarose gel electrophoresis after ion-exchange chromatography on Q-Sepharose and quantified by densitometry. The determination of serum HA was performed by ELISA. After the sinovial liquid proteolysis the CS and the HA have been equally identified for eletrophoresis and quantified by densitometry. In the beginning of this study the avarage of urinary concentrations of CS, DS, HS and total GAGs (2.96 mg/l, 0.66 mg/l, 0.42 mg/l, 4.05 mg/l respectively) have been inferior the urinary averages, right after the treatment (5.38 mg/l, 1.30 mg/l, 0.96 mg/l, 7.64 mg/l respectively)(p< 0,05) and similar after 30 days (4.24 mg/l, 1.09 mg/l, 0.36 mg/l, 5.69 mg/l respectively). The HA serum concentration increased after treatment and the end of the experiment regarding the inicial values (10.61 ng/ml, 12.58 ng/ml, 21.08 ng/ml respectively) (p<0.05). The synovial fluid CS showed a similar behavior to the urinary CS (beginning: 76.13 µg/ml, end of treatment: 93.05 µg/ml, end of the experiment: 61.61 µg/ml). But the synovial fluid HA did not change significantly (beginning: 440.07 µg/ml, end of treatment: 351.15 µg/ml, end of the experiment: 375.99 µg/ml) despite the tendency to decrease. It was concluded that oral administration of glucosamine, CS and HA associated shows a tendency to increase the urinary GAGs and CS in the synovial fluid immediately after treatment, with reduction in 30 days; increase of serum HA and maintenance of the concentration of HA in synovial fluid, with a tendency to decrease.
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Efeitos da administração oral de glucosamina e condroitim sulfato associados ao ácido hialurônico em cavalos com osteoartrite / Effects of oral administration of glucosamine sulfate and chondroitin sulfate associated with hyaluronic acid in horses with osteoarthritisJoyce Martins Coelho 17 July 2009 (has links)
A osteoartrite é a causa mais comum de claudicação em cavalos e, frequentemente está associada com a queda de desempenho e abandono precoce das atividades esportivas. Numerosos estudos têm investigado o potencial da função dos condroprotetores na desaceleração do processo degenerativo e no reparo da cartilagem articular. A finalidade deste estudo foi investigar o efeito da administração oral de glucosamina, condroitim sulfato e ácido hialurônico associados sobre a evolução clínica, radiológica, e glicosaminoglicanos (GAGs) da urina, do líquido sinovial e sérico de cavalos acometidos por osteoartrite. Foram utilizados seis equinos, com idades entre seis e doze anos, machos ou fêmeas, submetidos ao mesmo manejo nutricional. Estes animais receberam doses diárias de condroitim sulfato (CS) (2,8 g), ácido hialurônico (AH) (0,1g), glucosamina (3,1 g), via oral, por 25 dias. Exames clínicos e radiográficos foram realizados anteriormente e após o tratamento. Amostras de urina, líquido sinovial, e sangue foram coletados antes da primeira administração do suplemento; a cada 5 dias durante o tratamento e a cada 7 dias após o tratamento, durante 55 dias. Os glicosaminoglicanos urinários foram identificados por eletroforese em gel de agarose após cromatografia de troca iônica e quantificados por densitometria. A determinação de AH sérico foi realizada por ELISA. Após proteólise do líquido sinovial o CS e a AH foram igualmente identificados por eletroforese e quantificados por densitometria. No início do experimento, as concentrações médias urinárias de CS, DS, HS e GAGs totais (2,96 mg/l, 0,66 mg/l, 0,42 mg/l, 4,05 mg/l respectivamente) foram inferiores as médias urinárias, logo após o tratamento (5,38 mg/l, 1,30 mg/l, 0,96 mg/l, 7,64 mg/l respectivamente) (p<0,05) e similares após 30 dias (4,24 mg/l, 1,09 mg/l, 0,36 mg/l, 5,69 mg/l). A concentração sérica de AH aumentou após o tratamento e ao final do experimento em relação do início dos mesmos (10,61 ng/ml, 12,58 ng/ml, 21,08 ng/ml respectivamente) (p<0,05). O CS do líquido sinovial apresentou comportamento similar ao CS urinário (início: 76,13 µg/ml, final do tratamento: 93,05 µg/ml, final do experimento: 61,61µg/ml). Já o AH no líquido sinovial não sofreu alterações significativas (início: 440,07 µg/ml, final do tratamento: 351,15 µg/ml, final do experimento: 375,99 µg/ml) apesar da tendência a diminuição. Concluiu-se que a administração oral de glucosamina, CS e AH associados apresenta uma tendência ao aumento dos GAGs urinários e do CS do líquido sinovial imediatamente após o tratamento, com diminuição até 30 dias; aumento sérico do AH e manutenção da concentração de AH no líquido sinovial, com tendência a diminuição. / Osteoarthritis is the most common cause of lameness in horses, and often is frequently associated with poor performance and early end of sports activities. Numerous studies have investigated the potential function of chondroprotective drugs in slow down the degenerative process and helping to repair the joint cartilages. The purpose of this study was to investigate the effect of oral administration of glucosamine, chondroitin sulfate and hyaluronic acid associated with the clinical, radiological, and urinary glycosaminoglycans (GAGs), in the serum and synovial fluid of horses affected by osteoarthritis. We used six horses, aged between six and twelve years old, male or female, undergoing the same nutritional management. These animals received daily doses of condroitim sulfate (CS) (2.8 g), hyaluronic acid (HA) (0.1 g), glucosamine (3.1 g), oral route, for 25 days. Clinical and radiographic examinations were performed before and at the end of treatment. Samples of urine, synovial fluid and serum were collected before the first administration of the supplement, and every 5 days during the treatment and every 7 days after the end of the treatment, for a total of 55 days. The urinary glycosaminoglycans have been identified for agarose gel electrophoresis after ion-exchange chromatography on Q-Sepharose and quantified by densitometry. The determination of serum HA was performed by ELISA. After the sinovial liquid proteolysis the CS and the HA have been equally identified for eletrophoresis and quantified by densitometry. In the beginning of this study the avarage of urinary concentrations of CS, DS, HS and total GAGs (2.96 mg/l, 0.66 mg/l, 0.42 mg/l, 4.05 mg/l respectively) have been inferior the urinary averages, right after the treatment (5.38 mg/l, 1.30 mg/l, 0.96 mg/l, 7.64 mg/l respectively)(p< 0,05) and similar after 30 days (4.24 mg/l, 1.09 mg/l, 0.36 mg/l, 5.69 mg/l respectively). The HA serum concentration increased after treatment and the end of the experiment regarding the inicial values (10.61 ng/ml, 12.58 ng/ml, 21.08 ng/ml respectively) (p<0.05). The synovial fluid CS showed a similar behavior to the urinary CS (beginning: 76.13 µg/ml, end of treatment: 93.05 µg/ml, end of the experiment: 61.61 µg/ml). But the synovial fluid HA did not change significantly (beginning: 440.07 µg/ml, end of treatment: 351.15 µg/ml, end of the experiment: 375.99 µg/ml) despite the tendency to decrease. It was concluded that oral administration of glucosamine, CS and HA associated shows a tendency to increase the urinary GAGs and CS in the synovial fluid immediately after treatment, with reduction in 30 days; increase of serum HA and maintenance of the concentration of HA in synovial fluid, with a tendency to decrease.
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