Thesis (Ph. D.)--University of Washington, 1996. / Vita. Includes bibliographical references (leaves [54]-67).

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [176]-197).

Thesis (Ph.D.)--University of Alabama at Birmingham, 2008. / Title from first page of PDF file (viewed on June 23, 2009). Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (p. [119]-127).

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [103]-137).

Thesis (Ph. D.)--University of Washington, 2005. / Vita. Includes bibliographical references (leaves 163-179).

Thesis (Ph.D. in Molecular Biology) -- University of Colorado, 2006. / Typescript. Includes bibliographical references (leaves 126-147). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;

Edited by Samuel S. Epstein, with associate editors: Joshua Lederberg [and others] / "Based on a symposium cosponsored by the Center for Studies of Narcotic and Drug Abuse, NIMH, and by the Environmental Mutagen Society, San Francisco, October 29 and 30, 1969." / Includes bibliographies.

Submitted by ALLISON KLEITON DOS ANJOS null (allison__anjos@hotmail.com) on 2018-01-15T10:50:34Z No. of bitstreams: 1 TESE FINAL ALLISON.pdf: 3595248 bytes, checksum: 33a447746191e365878b383ffd929cc8 (MD5) / Rejected by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br), reason: Prezado Allison Kleiton dos Anjos, Solicitamos que realize uma nova submissão seguindo as orientações abaixo: - Resumo em língua estrangeira (inglês) - Faltou o Abstract no documento enviado. Este item é elemento obrigatório de acordo com as normas de trabalhos do seu Programa de Pós Graduação e deve vir logo após o Resumo em Português. Agradecemos a compreensão e aguardamos o envio do novo arquivo. Atenciosamente, Biblioteca Campus Rio Claro Repositório Institucional UNESP on 2018-01-15T16:52:52Z (GMT) / Submitted by ALLISON KLEITON DOS ANJOS null (allison__anjos@hotmail.com) on 2018-01-15T18:30:08Z No. of bitstreams: 1 TESE_FINAL_ALLISON.pdf: 4473839 bytes, checksum: ec9f453166f94072eff2f68fc43791e7 (MD5) / Approved for entry into archive by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br) on 2018-01-15T18:37:51Z (GMT) No. of bitstreams: 1 anjos_ak_dr_rcla.pdf: 4115054 bytes, checksum: 5a5c208184ffdb419015062384640e33 (MD5) / Made available in DSpace on 2018-01-15T18:37:51Z (GMT). No. of bitstreams: 1 anjos_ak_dr_rcla.pdf: 4115054 bytes, checksum: 5a5c208184ffdb419015062384640e33 (MD5) Previous issue date: 2017-12-15 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Os hemipteros da infraordem Cicadomorpha são representados por aproximadamente 30.000 espécies de insetos sugadores distribuídos mundialmente. Apesar de se destacarem por causarem muitos prejuízos na agricultura e pecuária pouco se sabe sobre a variabilidade genética e cromossômica desses animais que apresentam cromossomos holocentricos. Os DNAs repetitivos são uma ferramenta útil em estudos de diversificação cariotípica, organização e evolução dos genomas. Deste modo, este trabalho teve como objetivo contribuir com o conhecimento sobre a dinâmica dos cariótipos de representantes de Cicadomorpha e a organização de DNAs repetitivos, especificamente: I. analisar o cariótipo de espécies pertencentes a quatro famílias de Cicadomorpha, bem como caracterizar a heterocromatina constitutiva quanto a riqueza de pares de base; II. inferir a respeito da dinâmica evolutiva de famílias gênicas por meio do mapeamento cromossômico; III. analisar a organização cromossômica e testar a conservação interespecífica da sequência telomérica TTAGGn do pool de DNAs repetitivos obtidos (fração C0t) de espécies do gênero Mahanarva e das sequências teloméricas; IV. Analisar o satelitoma de Mahanarva quadripunctata e comparar os diferentes DNAs satélites de seu genoma com o de outras espécies do gênero visando entender a organização e evolução dos satDNAs neste grupo. Os dados obtidos revelam ampla variabilidade cromossômica entre as distintas famílias, causada principalmente por fusões cromossômicas, entretanto dentro de uma mesma família os cariótipos tendem a apresentar menos variações ao nível macrocromossômico. Embora os Cicadomorpha apresentem variabilidade em números diplóides a organização dos DNAs repetitivos é bastante conservada, mesmo em famílias distantes filogeneticamente, sugerindo estabilidade. Alguns dados foram analisados baseados na filogenia da infraordem, sendo sugerido os possíveis padrões ancestrais. Além disso, as possíveis causas da variabilidade em relação aos padrões modais são sugeridos. Os dados apresentados são um avanço no conhecimento da organização de DNAs repetitivos em Cicadomorpha, sendo para algumas sequências a primeira vez que se realiza algum estudo. / The hemipterans of the Cicadomorpha infraorder are represented by approximately 30.000 species of sucking insects distributed worldwide. Although they stand out by cause many damages in agriculture and livestock, they are poorly studied regarding the genetic and chromosomal variability of these animals that present holocentric chromosomes. Repetitive DNAs are a useful tools in studies of karyotypic diversification, organization and evolution of genomes. The aim of this work was to contribute with knowledge about the dynamics of the karyotypes of Cicadomorpha and the organization of repetitive DNAs, specifically: I. Analyze the karyotype of species belonging to four families of Cicadomorpha, as well as to characterize the constitutive heterochromatin regarding base pairs richess; II. Infer about the evolutionary dynamics of multigene families through the chromosomal mapping; III. Analyze the chromosomal organization and test the interspecific conservation of the telemetric repeat TTAGGn from the pool of repetitive DNA fraction (C0t fraction) of Mahanarva species and telomeric sequences; IV. Analyze the satellitome of Mahanarva quadripunctata and compare the different satellite DNAs of its genome with that from other species of the genus in order to understand the organization and evolution of satDNAs in this group. The data obtained reveal a wide chromosomal variability among the different families, caused mainly by chromosomal fusions, however within a same family the karyotypes tend to present less variations at the macro-chromosomal level. Although Cicadomorpha exhibit variability in diploid numbers, the organization of repetitive DNAs is highly conserved, even in phylogenetically distant families, suggesting stability. Some data were analyzed based on the phylogeny of the infraorder, suggesting possible ancestral patterns. In addition, the possible causes of variability relative to modal patterns are suggested. The data presented is an advance in the knowledge of the organization of repetitive DNAs in Cicadomorpha, being for some sequences the first time that some study is done. / FAPESP: 2014/06226-3.

abstract: The Philadelphia chromosome in humans, is on oncogenic translocation between chromosomes 9 and 22 that gives rise to the fusion protein BCR-Abl. This protein is constitutively active resulting in rapid and uncontrolled cell growth in affected cells. The BCR-Abl protein is the hallmark feature of chronic myeloid leukemia (CML) and is seen in Philadelphia-positive (Ph+) acute lymphoblastic leukemia (ALL) cases. Currently, the first line of treatment is the Abl specific inhibitor Imatinib. Some patients will, however, develop resistance to Imatinib. Research has shown how transformation of progenitor B cells with v-Abl, an oncogene expressed by the Abelson murine leukemia virus, causes rapid proliferation, prevents further differentiation and produces a potentially malignant transformation. We have used progenitor B cells transformed with a temperature-sensitive form of the v-Abl protein that allows us to inactivate or re-activate v-Abl by shifting the incubation temperature. We are trying to use this line as a model to study both the progression from pre-malignancy to malignancy in CML and Imatinib resistance in Ph+ ALL and CML. These progenitor B cells, once v-Abl is reactivated, in most cases, will not return to their natural cell cycle. In this they resemble Ph+ ALL and CML under Imatinib treatment. With some manipulation these cells can break this prolonged G1 arrested phenotype and become a malignant cell line and resistant to Imatinib treatment. Cellular senescence can be a complicated process requiring inter-play between a variety of players. It serves as an alternate option to apoptosis, in that the cell loses proliferative potential, but does not die. Treatment with some cancer therapeutics will induce senescence in some cancers. Such is the case with Imatinib treatment of CML and Ph+ ALL. By using the S9 cell line we have been able to explore the possible routes for breaking of prolonged G1 arrest in these Ph+ leukemias. We inhibited the DNA damage sensor protein ataxia telangiectasia mutated (ATM) and found that prolonged G1 arrest in our S9 cells was broken. While previous research has suggested that the DNA damage sensor protein ataxia-telangiectasia mutated (ATM) has little impact in CML, our research indicates that ATM may play a role in either senescence induction or release. / Dissertation/Thesis / M.S. Microbiology 2011