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Yes / Lakeshore and wetland settlements of the Circum-Alpine region are well known for their excellent preservation of organic remains and their potential for accurate dating through dendrochronology. This settlement tradition spans from the Neolithic to the Early Iron Age, though several hiatuses in lake-dwelling construction are observed. Traditional models for the abandonment of lake settlements rely upon climatically deterministic models, linking declining climatic conditions to increasing lake-levels, which would have impacted upon settlements and forced the inhabitants to relocate. Recent studies of Neolithic lake-dwellings have indicated that social factors also influenced the development of these settlements, while the ‘social biography’ of settlements has been an area of increasing interest in terrestrial settlements. A review of selected Late Bronze Age (LBA) lake settlement illustrates the development sequence seen at many lake-dwellings from across the Circum-Alpine region. The proposal of a biographical model linking cultural influences to the development sequence observed in LBA lake-dwellings, and to the choice to abandon areas and relocate villages, offers further insights into the development of enigmatic settlements. / Swiss National Science Foundation

No / After more than 3500 years of occupation in the Neolithic and Bronze Age, the many lake-dwellings’ around the Circum-Alpine region ‘suddenly’ came to an end. Throughout that period alternating phases of occupation and abandonment illustrate how resilient lacustrine populations were against change: cultural/environmental factors might have forced them to relocate temporarily, but they always returned to the lakes. So why were the lake-dwellings finally abandoned and what exactly happened towards the end of the Late Bronze Age that made the lake-dwellers change their way of life so drastically? The new research presented here draws upon the results of a four-year-long project dedicated to shedding light on this intriguing conundrum. Placing a particular emphasis upon the Bronze Age, a multidisciplinary team of researchers has studied the lake-dwelling phenomenon inside out, leaving no stones unturned, enabling identification of all possible interactive socio-economic and environmental factors that can be subsequently tested against each other to prove (or disprove) their validity. By re-fitting the various pieces of the jigsaw a plausible, but also rather unexpected, picture emerges.

Conselho Nacional de Desenvolvimento Científico e Tecnológico / We present a multi-wavelength study of the stellar content of 11 optical/infrared Southern Galactic Hii regions with 10 h < α(J2000) < −17 h and −65° < δ(J2000) < −35°. Nine optical objects with no published or uncertain distances were examined in order to identify their ionising sources and to determine their distances, whereas young stellar objects (YSOs) and main sequence ionising stars were photometrically classified in the two infrared clusters. We carried out optical spectroscopy and UBV photometry of the stellar content of these Hii regions and obtained the distance of individual stars by spectroscopic parallax. To avoid using a fixed value for the total-to-selective extinction ratio RV , the reddening AV was determined directly by the colour-difference method. We classified as types O or B 24 out of the 31 stars for which optical spectra were obtained. In particular, we identified two new O stars in RCW98 and RCW99. The values for reddening obtained correspond to a mean hRV i = 3.44, which is about 10% higher of the mean value found for field stars over all directions of the Galaxy. For the Hii regions NGC3503, NGC6334, RCW55, RCW87, RCW98 e RCW99, we obtained more precise distances calculated as the median of the spectroscopic parallaxes obtained for two to six different stars in each nebulae, with an internal dispersion of less than 5%. Among the objects more obscured in the Norma region, we analysed the stellar content of the East part of the large complex of Hii regions GAL331.5-00.1, which contains eight bright extended radio sources. This study comprises the infrared clusters [DBS2003] 156 and [DBS2003] 157, respectively associated to Hii regions GAL331.11-00.51 and GAL331.31-00.34. In order to isolate the brightest 2MASS sources of their unresolved companions, we carried out JHK photometry with resolution better than 2MASS data. 47 Near-infrared (NIR) sources with Ks-band excess were identified following usual methods. Other 70 YSOs were also identified by using Mid-IR on-line data of the GLIMPSE survey. The search for radial-velocity measurements in the literature and the similarity between the stellar population explored have indicated the two regions as physically associated. With the determination of the spectroscopic parallaxes of four O-type and two B-type stars spread over the both clusters, this hypothesis was verified. The parallaxes of these 6 stars returned very compatible distances (hdhelioci = 3.30 ± 0.29 kpc). The Near- and Mid-IR counterparts of the IRAS source 16085-5138 was found close to the field of [DBS2003] 157. This source has showed typical colours of a Ultra-compact ii region (UCHii) and spectral index α = 3.6 between 2 and 25 μm, which is typical of YSOs immersed in protostellar envelopes. A lower limit to the bolometric luminosity of the protostar embedded was computed as L = 7.7×103L⊙ (M = 10M⊙), which corresponds to a BO-B1 zero-age star. The cluster [DBS2003] 157 was found to be spread over all ∼4′ × 4′ region demarcated by a intense shell-like dust emission, where a secondary massive star formation is going on, likely as a result of the interaction between this dust and stellar winds. / Apresentamos aqui um estudo multibanda do conteúdo estelar de onze regiões Hii do Hemisfério sul com 10 h < α(J2000) < −17 h e −65◦ < δ(J2000) < −35◦. Em 9 objetos ópticos, com valores de distância discrepantes ou sem nenhuma determinação anterior publicada, visamos identificar as fontes ionizantes e determinar suas distâncias, enquanto que, no estudo de dois outros objetos no infravermelho, buscamos não apenas identificar as fontes ionizantes, mas também objetos estelares jovens (YSOs) em seus campos. Nos primeiros, realizamos espectroscopia no óptico e fotometria UBV de seus conteúdos estelares e determinamos as distâncias das estrelas massivas por meio da paralaxe espectroscópica. A fim de evitar o uso de um valor fixo para a razão entre a extinção total e a seletiva à banda V , RV , a extinção AV na direção de cada estrela foi determinada diretamente através no método das diferenças de cor. De um total de 31 estrelas espectroscopicamente estudas, 24 foram classificadas como tipos O ou B, sendo duas novas estrelas tipo O encontradas em RCW98 e RCW99. As estimativas de AV implicaram um valor médio de hRV i = 3.44. Este resultado supera em 10% o valor médio encontrado para estrelas de campo sobre todas as direções da Galáxia. Para as regiões Hii NGC3503, NGC6334, RCW55, RCW87, RCW98 e RCW99, distâncias mais precisas foram estimadas como o valor mediano da paralaxe espectroscópica de 2 a 6 diferentes estrelas ionizantes, resultando em uma dispersão interna menor que 5%. Dentre os objetos mais obscurecidos pela extinção interestelar da região de Norma, analisamos o conte´udo estelar da região leste do grande complexo de regiões Hii brilhantes em radiofrequências GAL331.5-00.1. A área estudada engloba os aglomerados infravermelhos [DBS2003] 156 e [DBS2003] 157, respectivamente associados às regiões Hii GAL331.11-00.51 e GAL331.31-00.34. Observações fotométricas nas bandas J, H e Ks, com mais alta resolução que a fotometria 2MASS, foram realizadas nas direções desses objetos, o que possibilitou isolar as fontes infravermelhas mais brilhantes de estrelas companheiras não resolvidas e selecionar potenciais estrelas ionizantes para subsequente espectroscopia. 47 fontes com excesso de emissão intrínseca na banda Ks, típico em YSOs, foram identificadas seguindo os métodos usuais de análise da fotometria no IR próximo (NIR). Outros 70 YSOs foram identificados no IR médio (Mid-IR) usando dados do survey GLIMPSE. A pesquisa por medidas de velocidade radial na direção desses dois objetos e a semelhança das populações estelares indicou que as duas sub-regiões estudas deveriam estar fisicamente associadas. Esta hipótese foi reafirmada com a determinação da paralaxe espectroscópica de 4 estrelas tipo O e outras 2 tipo B nos dois aglomerados, que retornaram valores de distâncias heliocêntricas bastante compatíveis (hdhelioci = 3.30 ± 0.29 kpc). A contrapartida nos NIR e Mid-IR da fonte IRAS 16085-5138 foi encontrada junto ao aglomerado [DBS2003] 157. Esta fonte apresentou cores típicas de uma região Hii Ultracompacta (UCHii) e índice espectral entre 2 e 25 μm de α = 3.6, típico de YSO imerso em um envelope protoestelar. Um limite inferior para a luminosidade bolométrica da protoestrela embebida foi estimado em L = 7.7×103L⊙ (M = 10M⊙), o que corresponde a uma estrela de idade zero na faixa de BO-B1. O aglomerado [DBS2003] 157 mostrou-se estar espalhado sobre toda uma região de ∼4′ ×4′, demarcada por intensa emissão de poeira quente e espacialmente distribuída como uma nuvem em forma de concha.

Plasmodien spp. sind obligat intrazellulär lebende Parasiten, welche einen evolutionär konservierten aktinabhängigen molekularen Motor für die Fortbewegung und den Wirtszellein- und -austritt nutzen. In dieser Arbeit werden die Aktinregulatoren Adenylyl- Zyklase- assoziierte Protein (C-CAP), Profilin sowie die Aktin depolymerizierenden Faktoren 1 und 2 (ADF1, ADF2) in Plasmodium berghei charakterisiert. Die Geninaktivierung von C-CAP besitzt keinen Einfluss auf die Entwicklung von pathogenen Blutstadien. C-cap(-) Ookineten bewegen sich jedoch deutlich langsamer, sind aber in der Lage den invertebraten Wirt zu infizieren. Defekte treten während der extrazellulären Replikationsphase im Mosquito auf und führen zu Abbruch des Lebenszykluses. Die erfolgreiche Komplementierung der Defekte mit dem orthologen Gen aus Cryptosporidium parvum CpC-CAP bestätigt die funktionale Redundanz zwischen beiden Proteinen. Profilin, als ein weiteres G-Aktin bindendes Protein, ist hingegen nicht in der Lage die Defekte des c-cap(-) Parasiten auszugleichen. Mittels transgener Parasiten welche ein C-CAPmCherry Fusionsprotein exprimieren, wird das C-CAP Protein im Zytoplasma lokalisiert. Erstmals wird mit dieser Arbeit ein G-Aktin bindendes Protein, C-CAP beschrieben, welches eine essentielle Funktion während der Oozystenreifung in Plasmodium berghei besitzt. Die Transkription der Aktinregulatoren Profilin, ADF1 und ADF2 wird in Sporozoiten drastisch herunterreguliert und Profilin kann als Protein nicht mehr nachgewiesen werden. Um die Funktion von C-CAP und Profilin zu überprüfen, wurden beide Proteine spezifisch in Sporozoiten überexprimiert. Diese Parasiten sind nicht in der Lage die Speicheldrüsen des Wirtes zu besiedeln, was zum Abbruch des Lebenszykluses führt. Anhand dieser Ergebnisse entwickele ich ein „minimalistisches“ Model zur Beschreibung der Aktinregulation in Sporozoiten in welchem das ADF1 als regulatorisches Protein im Mittelpunkt steht. / Plasmodium spp. are obligate intracellular parasites, which employ an conserved actin-dependent molecular motor machinery that facilitates their motility, host cell invasion and egress. In this work I report implications of the actin-regulators adenylyl cyclase-associated protein (C-CAP), profilin and actin depolymerization factor 1 and 2 (ADF1, ADF2) in distinct and previously unanticipated cellular processes during the life cycle of in the rodent malarial parasite Plasmodium berghei. Fluorescent tagging of the endogenous C-CAP genetic locus with mCherry revealed cytosolic distribution of the protein. Gene deletion demonstrates that the G-actin binding protein C-CAP is entirely dispensable for the pathogenic blood stages. Ookinetes show reduced motility, but are competent infecting the mosquito host. Defects emerging in the extracellular replication phase, leading to attenuation of oocyst maturation. Successful trans-species complementation with the C. parvum C-CAP ortholog, rescues the c-cap(-) phenotype and proves functional redundancy. The actin regulator profilin fails to rescue the defects of c-cap(-) parasites, despite sharing its actin sequestering activity with C-CAP. Taken together, C-CAP is the first G-actin sequestering protein of Plasmodium species that is not required for motility but performs essential functions during oocyst maturation. Characterization of the actin regulators profilin, ADF1 and ADF2 revealed dramatic transcriptional down-regulation and the absence of the profilin protein in sporozoites. To test whether G-actin binding proteins interfere with sporozoite functions, I ectopically overexpressed of profilin and C-CAP stage-specifically in sporozoites. This conducted to abolishment of salivary gland invasion and lifecycle arrest. Based on these unexpected findings and the available literature data, I developed a “minimalistic model” for actin regulation in sporozoites that predicts ADF1 as the main actin-turnover regulating factor.