Objectives of the present study were to evaluate neonatal Holstein heifer performance while supplemented with coccidiostat (CX), yeast (YST), β-glucan or some combination of these products in non-medicated milk replacer. Efficacy of these products on E. coli and coccidia shedding was also observed. No differences (P > 0.05) were observed among treatment groups with respect to performance data collected which included growth, feed intake and efficiency, fecal and respiratory and blood parameters. Heifers fed YST or YST + β-glucan had higher (P = 0.02 and P < 0.01, respectively) fecal E. coli concentrations compared to heifers fed CX, CX + YST, or β-glucan. No heifers showed clinical signs of coccidiosis and appeared to be healthy. These results suggest neonatal heifers may be supplemented with yeast derivatives without negative effects on growth or health performance.