My thesis tested efect of mycorrhizal fungy on herbal cutting. There was two terms propagation of four species. Syringa meyeri 'Palibin', Forsythia x intermedia 'Maluch', Cornus alba and Berberis thunbergii 'Rose Glow'. Experiment had two variantion and three repeats. Plants couldn't be nursed. Most of the results are inconclusive, because of the high losses. For species of Syringa meyeri 'Palibin' was demonstrated high influence of mycorrhizal fungi. There were also differences in terms. Overlay film had a positive impact only on Cornus alba. For species Cornus alba and Forsythia x intermedia 'Maluch' second term was more successful. When propagation of mycorrhiza has high potential.

This thesis deals with determination of content of vitamin C in various types of cornelian cherry (Cornus mas L.). Some basic information about Cornus mas L. and its various types are described in the literary part of this thesis. The summary of important components present at fruit and some possibilities of processing and utilization are described here too. Various methods of determination of vitamin C are presented at the end of the literary part. The main result of the experimental part of this thesis is the comparison of content of vitamin C in various types of cornelian cherry (Cornus mas L.). Reversed Phase-High Performance Liquid Chromatography was used as the main method. HPLC method of determination of vitamin C in various types of Cornus mas L. was completed with the orientation titration. Then total antioxidant capacity of fruit was evaluated by using of reaction of extracts of fruit with stable radical DPPH. The biggest amount of vitamin C was determined in the Fruchtal variety. The biggest total antioxidant capacity was determined in the Vydubecký variety.

This thesis deals with the determination of vitamin C in different varieties of cornelian cherry (Cornus mas) and mountain ash (Sorbus aucuparia). The theoretical part describes the botanical characteristics of both plants, the extension in the CZ and in the world, methods of reproduction, the content of meaningful health substances and the importance of both plants in food-processing industry. Attention is paid to vitamin C, which is in their fruits in high quantity. Conclusion of the theoretical part discusses various methods of determination of vitamin C. The aim of the experimental part was to comparison of the vitamin C content in different varieties cornelian cherry and mountain ash. The chosen method for the determination of vitamin C by high performance liquid chromatography (HPLC) with UV detection was first tested on standards. Selected validation parameters of this method was then determined. Validated method was used to determine the content of vitamin C in nine varieties cornelian cherry and six varieties of the mountain ash. For comparison were also tested some samples of the determination of total vitamin C after reducing dehydroascorbic acid by using dithiothreitol (DTT).

The aim of this diploma thesis is determination of selected metals in unconventional fruit species, specifically in cornelian cherry (Cornus mas), sea buckthorn (Hippophae rhamnoides) and medlar (Mespilus germanica).There is informationabout 16 analysed metals – As, B, Be, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Se, Sn, Pb, V, Zn. There are described analytical techniques for elemental analysis and methods for destruction biological material. Samples for analysis are prepared by using microwave mineralization and ICP-MS for detection. This is described in the experimental part of the diploma thesis. Analysis confirmed that unconventional fruits are rich sources of trace elements.

Introduction of invasive species can alter seed fate predictions made by the Janzen-Connell Escape Hypothesis (JCEH). The JCEH states that there is a suitable region around a plant that is ideal for seed germination, growth, and recruitment. Seeds dispersed too close to the maternal plant are subject to competition from the maternal individual and perhaps density-dependent predation, whereas seeds dispersed further away may end up in suboptimal habitats. Invasive species may change the amount of these suitable habitats for native plants by creating unsuitable light environments and as a result, may influence the size of the ideal recruitment zone surrounding a parent plant. This study examines the extent to which the invasive grass species, Microstegium vimineum, influences recruitment of the understory tree, Cornus florida. In general, M. vimineum was found to reduce both germination and early seedling success and may have significant consequences for future forest structure.

Flowering dogwood (Cornus florida L.) populations have experienced severe declines caused by dogwood anthracnose in the past three decades. Mortality has ranged from 48 to 98%, raising the concern that genetic diversity of this native tree has been reduced significantly. Microsatellite data were used to evaluate the level and distribution of genetic variation throughout much of the native range of the tree. In the first conducted study, we found that genetic variation in areas affected by anthracnose was as high as or higher than areas without die-offs. We found evidence of four widespread, spatially contiguous genetic clusters. However, there was little relationship between geographic distance and genetic difference. These observations suggest that high dispersal rates and large effective population sizes have so far prevented rapid loss of genetic diversity. The effects of anthracnose on demography and community structure are likely to be far more consequential than short-term genetic effects. The second study examined levels and distribution of genetic variation of C. florida throughout Great Smoky Mountains National Park (GSMNP). Significant genetic structure at both landscape and local levels were found. We infer that two genetic clusters exist within the park, mostly separated by the main dividing ridge of the Great Smoky Mountains. The differentiation is statistically significant, but subtle, with gene flow evident through low-elevation corridors. It seems unlikely that recent demographic dynamics have resulted in a depletion of genetic variation in flowering dogwoods.

The theme of this diploma thesis is an assessment of given nutritional parameters in selected kinds of lesser known fruits, specifically 10 varieties of sea buckthorn (Hippophae rhamnoides L.), 12 varieties of cornelian cherry (Cornus mas L.) and 6 varieties of blue honeysuckle (Lonicera caerulea var. kamtschatica). Harvests of two consecutive years (2011 and 2012) were analyzed in the case of blue honeysuckle. The theoretical part was focused on historical, morphological, taxonomic and nutritional aspects of individual plants and food or pharmaceutical utilization. The considerable part of the thesis is devoted to the characterization and the possibilities of determining the contained substances, namely ascorbic acid and polyphenolic compounds, especially anthocyanins. The content of the experimental part was a description of the methods and the results of the analysis of total solids, titratable acidity, total phenolics, total anthocyanins and ascorbic acid in fruits of the selected varieties of the above mentioned fruit species. Based on the measured results, the different varieties of fruits were compared to each other and evaluated.