Thesis (M.S.P.H.)--University of Michigan, 1939.

Thesis (doctoral)--Universität Tübingen, 1934. / At head of title: Aus dem Hygienischen Institut der Universität Tübingen. "Lebenslauf": p. 19. Includes bibliographical references.

Made available in DSpace on 2014-06-11T19:32:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-02Bitstream added on 2014-06-13T19:03:32Z : No. of bitstreams: 1 pardo_rb_dr_jabo.pdf: 1134204 bytes, checksum: f5af7219cd5d0c42c86945ad29a46afe (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Os exames de palpação, inspeção e teste da caneca de fundo escuro, bem como o California Mastitis Test (CMT) foram realizados em 735 quartos mamários de búfalas leiteiras pesquisa de alterações clínicas e subclínicas nos quartos e em suas respectivas secreções. Independentemente dos resultados encontrados, foram coletadas amostras de leite para análises microbiológicas e de contagem de células somáticas (CCS). O isolamento microbiológico foi realizado em ágar sangue de ovino 5% e em ágar MacConkey, com 72 horas de incubação em aerobiose a 37oC. Foram procedidas, a cada 24 horas, leitura e identificação dos isolamentos. A ocorrência de quadros de mastite clínica foi representada por um único caso (0,14%) entre os 735 quartos mamários analisados, ocorrendo na fase inicial da lactação e envolvendo microrganismos de origem ambiental (Morganella morganii, Enterobacter aerogenes). Foram considerados quadros de mastite subclínica aqueles com resultados positivos ao CMT acompanhados de exame microbiológico positivo. A freqüência observada de quadros de mastite subclínica entre os 734 quartos mamários estudados foi de 20,03%, dos quais isolou-se principalmente microrganismos contagiosos. Os resultados negativos ao CMT predominaram durante todo o estudo (69,07%), independente da fase de lactação ou estação do ano consideradas, tendo sido observados resultados positivos do exame microbiológico em 57,58% das amostras CMT negativas. Foi elevada a freqüência de microrganismos classificados como contagiosos entre as amostras de leite analisadas, independente da estação do ano e da fase de lactação, entre os quais prevaleceu o Corynebacterium spp. (47,67%), entre os 579 microrganismos pertencentes a 19 gêneros bacterianos crescidos em cultura pura ou em associação. Considerando-se a falta de homogeneidade entre as variâncias, as contagens de células... / A total of 735 mammary quarters from buffalo cows were submitted to inspection, palpation, strip cup test and California Mastitis Test (CMT) in order to diagnose clinical and subclinical mastitis. Independent of the observed results, milk samples were collected from each mammary quarter to microbiological exams and automatic somatic cell counts (SSC). Milk samples were inoculated in 5% ovine blood agar and MacConkey agar and incubated for 72 hours under 37oC and aerobic condition. Growth observations and identifications were performed every 24 hours. One (0,14%) out of 735 mammary quarters studied presented clinical mastitis. It occurred in first stage of lactation (0 to 60th day post calving) and environmental microorganisms were isolated (Morganella morganii, Enterobacter aerogenes). Mammary quarters presenting positive results to CMT and microbiological exams were considered as subclinical mastitis cases and these represented 20,03% among 734 studied samples. CMT negative results were the most frequent observed during the study (69,07%), independent of lactation stage or season of the year considered. Among these CMT negative milk samples, 57,58% were positive in microbiological exams. There was a high occurrence of contagious microorganisms among the 579 identified bacteria from 19 different genus, isolated in pure or in association. Corynebacterium spp. was the most prevalent (47,67%), in every lactation stage or season of the year. Considering the absence of a normal distribution, somatic cell counts were submitted to a logarithmic transformation, log2(CCS/100)+3, originating the transformed somatic cell count (TSCC). There were statistically significant differences between TCCS means from milk samples with positive (2,23) and negative (1,71) results to CMT. Such differences were also observed in milk... (Complete abstract click electronic access below)

Corynebacterium pseudotuberculosis is a pathogenic bacterium which cause suppurative infection mainly in sheep, goats, horses, and other species. It plays a major role in the economy of sheep production worldwide. It produces a toxic phospholipase D which may be involved in pathogenesis. The relationship of physiologic differences between the two biovars of the organism to rates of growth and toxin production are not known. In this work, isolates of C.pseudotuberculosis were examined for relative ability to grow in brain heart infusion broth and a semidefined medium. The effects of carbohydrate source and the presence or absence of various ions were also examined. Results revealed no statistically significant differences between isolates or biovars in any characteristic studied, including growth rate, pH change during growth and PLD production. The semidefined medium provides a reasonable alternative to more complex media for further work in purification of PLD and study of its structure and function.

La linfadenitis granulomatosa es una enfermedad de significancía económica en alpacas, la cual es causada por Corynebacterium pseudotuberculosis, una bacteria gram positiva cuyo principal factor de virulencia es la exotoxina que es una fossolipasa D. El objetivo del presente trabajo fue probar la potencia de una vacuna elaborada a partir de precipitado proteico de C. Pseudotuberculosis, conteniendo la exotoxina, en un grupo de 20 ratones (grupo vacunado) quienes recibieron la vacuna y el inoculo con C. Pseudotuberculosis y compararlos con otro grupo de 20 ratones (grupo control) quienes recibieron placebo y el inoculo con C. Pseudotuberculosis. La inmunización del grupo vacunado con 16ug del precipitado proteico de C. Pseudotuberculosisindujo protección después de la inoculación intraperitoneal con 104 UFC de C. Pseudotuberculosis. La vacuna disminuyo los efectos tóxicos del C. Pseudotuberculosis, lo cual se observo con la disminución del numero y tamaño de abscesos de los animales del grupo vacunado (40%) comparado con las lesiones severas y generalizadas encontradas en los animales del grupo control (95%)que no fueron vacunados. Como parte de la metodología se desarrollo la técnica de PCR par la detección de C. Pseudotuberculosis en los animales con presencia de abscesos, en todos ellos salió positivo lo cual fue evidenciado con las bandas de DNA de 815bp demostrándose así q C. Pseudotuberculosis como agente causal de la infección.

Memoria para optar al Título Profesional de Médico Veterinario / Corynebacterium pseudotuberculosis es el microorganismo causante de la linfoadenitis caseosa en pequeños rumiantes, así como de la linfangitis ulcerativa y abscesos en equinos y bovinos, causando pérdidas económicas importantes ya que disminuye el rendimiento productivo del ganado. Uno de los principales factores de virulencia de esta bacteria es la presencia de una esfingomielinasa específica, la fosfolipasa D, que favorece la diseminación de la bacteria dentro del hospedero. En este estudio se determina la presencia del gen que codifica esta exotoxina y se cuantifica la capacidad hemolítica de la misma, en aislados de C. pseudotuberculosis obtenidos desde ovinos, caprinos y equinos. Como resultado se obtuvo que existen diferencias dependiendo del origen, lo que puede estar generando las características de expresión de la enfermedad, ya que las cepas de origen ovino tienen mayor capacidad hemolítica que las de otras especies. Se sugiere que la capacidad hemolítica de las diferentes cepas puede estar relacionada con presentaciones clínicas diferentes en las especies animales en estudio y se propone un método de medición cualitativa de hemólisis en placas de agar sangre

La linfadenitis granulomatosa es una enfermedad de significancía económica en alpacas, la cual es causada por Corynebacterium pseudotuberculosis, una bacteria gram positiva cuyo principal factor de virulencia es la exotoxina que es una fossolipasa D. El objetivo del presente trabajo fue probar la potencia de una vacuna elaborada a partir de precipitado proteico de C. Pseudotuberculosis, conteniendo la exotoxina, en un grupo de 20 ratones (grupo vacunado) quienes recibieron la vacuna y el inoculo con C. Pseudotuberculosis y compararlos con otro grupo de 20 ratones (grupo control) quienes recibieron placebo y el inoculo con C. Pseudotuberculosis. La inmunización del grupo vacunado con 16ug del precipitado proteico de C. Pseudotuberculosisindujo protección después de la inoculación intraperitoneal con 104 UFC de C. Pseudotuberculosis. La vacuna disminuyo los efectos tóxicos del C. Pseudotuberculosis, lo cual se observo con la disminución del numero y tamaño de abscesos de los animales del grupo vacunado (40%) comparado con las lesiones severas y generalizadas encontradas en los animales del grupo control (95%)que no fueron vacunados. Como parte de la metodología se desarrollo la técnica de PCR par la detección de C. Pseudotuberculosis en los animales con presencia de abscesos, en todos ellos salió positivo lo cual fue evidenciado con las bandas de DNA de 815bp demostrándose así q C. Pseudotuberculosis como agente causal de la infección.

The objective of this thesis was to better understand the epidemiology of caseous lymphadenitis (CLA) and its economic effects on the Australian sheep industries. The work also sought to apply this knowledge to better understand how to control the disease in Australian sheep flocks. The occurrence of CLA in some other species, as a comparison to CLA in sheep, is described in the literature review. However a comprehensive review of research on CLA in all livestock species is not needed to meet the aims of the thesis. The research used serological tests developed in the 1970s and ‘80s to examine the epidemiology and costs of CLA in Western Australian sheep flocks. Epidemiological techniques were used to determine the main risk factors associated with high incidences of CLA. Controlled experimentation was used to better understand the spread of CLA within flocks. Prevalence data collected at abattoirs and survey techniques were employed to better understand how CLA was being controlled in Australian sheep flocks. It was shown that toxin and cell wall ELISA tests for CLA were useful tools to study the spread of CLA in sheep flocks. Using the serological tests it was estimated that 80 per cent of CLA spread occurs after the second and third shearings. This knowledge was used to design a risk factor study which identified two significant factors associated with high CLA spread, that is in dips and close contact after shearing. These tests also facilitated the study of new CLA infection on wool production. It was found that sheep produce approximately five per cent less clean wool in the year in which they are infected. The main routes for the spread of CLA were from sheep with lung abscesses to those with skin cuts and through dips, rather than from an environment contaminated with Corynebacterium pseudotuberculosis bacteria. A survey of sheep producers found that the prevalence of CLA had decreased from over 50% in the 1970s to approximately 20% in the late 1990s. It also found that current vaccines were being used inefficiently. Large increases in the efficiency of control can be achieved by approximately 70 per cent of producers by using commercially available vaccines and the recommended vaccination programs of two

Ulm, Univ., Diss., 2008.

Universiẗat, Diss., 2004--Köln.