Grain feeding is an important part of a dairy feeding program. Grain supplies energy in a concentrated form and when fed as a supplement to forage permits cows to maintain high levels of production. Also, if the forage is of low quality, additional protein and other nutrients can be supplied with the grain in a concentrate mix. By feeding a balanced concentrate mix, according to individual production, each cow receives the necessary nutrients for production. Dairy cows are usually fed grain individually. During recent years dairy herds have increased in size and milking parlors have been developed in which to milk cows more efficiently. In milking parlors cows are generally fed grain individually. However, the cows are milked so rapidly that there is insufficient time for them to consume more than a few pounds of grain. Cows not consuming all of their grain, while being milked, are left in the stall until the remaining grain is eaten, thus delaying the milking operation or turned out without consuming the allotted amount of grain.

The relationship of quantity and quality of feed to milk production has been studied for many years. Feeding standards have been developed to systematize feeding of dairy cows according to their requirements. Recently, interest in efficiency of cows in converting feed nutrients into milk has increased.

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Early-lactating dairy cows mobilize body protein, primarily from skeletal muscle, to provide amino acids which are directed towards gluconeogenesis and milk protein synthesis. Propylene glycol (PG) is a precursor of ruminal propionate, and our hypothesis was that its dietary inclusion could attenuate skeletal muscle wasting by reducing amino acid-driven gluconeogenesis. The major objectives of this study were to delineate the effects of pre- and post-partum PG supplementation in transition dairy cows on whole-body nitrogen (N) balance, urinary 3-methylhistidine (3-MH) excretion, body composition, and gene expression profiles for the major protein degradation pathways in skeletal muscle. Sixteen pregnant cows (7 primiparous and 9 multiparous) were paired based on expected calving dates and then randomly assigned within each pair to either a basal diet (control) or basal diet plus 600 mL/d of PG (PG). Diets were fed twice daily for ad libitum intake, and PG was fed in equal amounts as a top dress. All measurements were conducted at 3 time intervals starting at d -14 ± 5, d 15 and d 38 relative to calving. Propylene glycol had no effect (P > 0.05) on whole-body N balance, urinary 3-MH excretion, and body composition. However, N balance was lower (P < 0.001) at d 15 and d 38, compared to d -14. Urinary excretion of 3-MH was lower at d -14 than at d 15 (P = 0.01) and d 38 (P = 0.001). Supplemental PG had no effect (P > 0.05) on body weight (BW), and all components of empty BW. On average, cows fed both diets mobilized 19 kg of body fat and 14 kg of body protein between d -14 and d 38. Supplemental PG had no effect on mRNA abundance in skeletal muscle for m-calpain (P = 0.96) and 14-kDa ubiquitin-carrier protein E2 (14-kDa E2) (P = 0.54); however, PG supplementation down-regulated mRNA expression for µ-calpain at d 15 (P = 0.02), and tended to down-regulate mRNA expression for ubiquitin at d 15 (P = 0.07) and proteasome 26S subunit-ATPase at d 38 (P = 0.097). Relative to calving, mRNA abundance for m-calpain (P = 0.02) and µ-calpain (P = 0.005) were higher at d 15 compared to d -14 and d 38. Messenger RNA abundance for ubiquitin (P = 0.07) and 14-kDa E2 (P = 0.005) were lower at d 38 compared to d 15. In summary, these results demonstrate that up-regulation of the Ca2+-dependent and ubiquitin-mediated proteolytic pathways are the mechanisms by which skeletal muscle wasting occurs in early-lactating cows. In addition, dietary supplementation with PG may down-regulate some of these proteolytic pathways, thereby potentially attenuating undesirable skeletal muscle wasting.

The objective of this study was to determine the effects of method of barley grain processing (dry-rolled vs. pelleted barley) and source of oilseed (ground canola vs. ground flaxseed), arranged as a 2 x 2 factorial, on feed intake, ruminal fermentation, nutrient flow to the duodenum, and milk production and composition in dairy cows. Eight Holstein cows (655 ± 69 kg; 83 ± 16 DIM) were used in a replicated 4 x 4 Latin square with 28-d periods. Cows in one square were fitted with ruminal and duodenal cannulae. Cows fed dry-rolled barley consumed 1.8 to 3.5 kg/d more (P = 0.02) DM than those fed pelleted barley; however, source of supplemental dietary fat had no effect on DM intake. Ruminal pH was lower (P = 0.045) in cows fed pelleted barley compared to those fed dry-rolled barley. Ruminal concentration of acetate was greater (P = 0.001), whereas ruminal concentration of propionate tended to be lower (P = 0.11), in cows fed dry-rolled barley compared to those fed pelleted barley; consequently, the acetate:propionate ratio was higher (P = 0.01) in cows fed dry-rolled barley compared to those fed pelleted barley. Ruminal concentration of total VFA was unaffected (P > 0.05) by diet. Source of dietary fat had no effect on ruminal digestion of OM, NDF, ADF or starch; however, ruminal starch digestion was slightly higher in cows fed pelleted barley compared to those fed dry-rolled barley (90.8 vs. 89.5%). Total dietary fatty acid intake was higher (P < 0.05) in cows consuming dry-rolled barley compared to those fed pelleted barley. Duodenal flow of C18:0 was lower, whereas that of C18:2n6c was higher (P < 0.05) in cows fed pelleted barley compared to those fed dry-rolled barley. Feeding flaxseed increased duodenal flows of C18:3n3, cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid compared to feeding canola. Milk yield was unaffected (P > 0.05) by diet; however, milk fat content was higher (P = 0.004) in cows fed dry-rolled barley compared to those fed pelleted barley. Milk fat content of C18:3 was higher (P = 0.005) in cows fed canola compared to those fed flax. Milk fat content of C18:3 and cis-9, trans-11 C18:2 were higher in cows fed pelleted barley compared to those fed dry-rolled barley with flax as the source of oilseed, but not with canola (interaction, P < 0.01). Milk fat content of saturated fatty acids decreased (P < 0.001) and that of polyunsaturated fatty acids increased (P = 0.003) in cows fed pelleted barley compared to those fed dry-rolled barley. In summary, milk fatty acid profiles were altered by method of grain processing and source of oilseed.

The Painter’s Wife and Other Stories is a novella and a collection of short fiction focusing on the lives of women and men in the contemporary Western United States. In their exploration of Western life, the novella and short stories subvert the popular mythology of the West. The novella and stories are set in one of three Colorado settings: the city of Denver, the rural Western slope, and the mountain communities of the Western Slope. Beyond being linked by region, the stories are also loosely linked by characters. Characters from one story are mentioned or make brief appearances in others, reinforcing the idea of a people connected by community and landscape.

Early-lactating dairy cows mobilize body protein, primarily from skeletal muscle, to provide amino acids which are directed towards gluconeogenesis and milk protein synthesis. Propylene glycol (PG) is a precursor of ruminal propionate, and our hypothesis was that its dietary inclusion could attenuate skeletal muscle wasting by reducing amino acid-driven gluconeogenesis. The major objectives of this study were to delineate the effects of pre- and post-partum PG supplementation in transition dairy cows on whole-body nitrogen (N) balance, urinary 3-methylhistidine (3-MH) excretion, body composition, and gene expression profiles for the major protein degradation pathways in skeletal muscle. Sixteen pregnant cows (7 primiparous and 9 multiparous) were paired based on expected calving dates and then randomly assigned within each pair to either a basal diet (control) or basal diet plus 600 mL/d of PG (PG). Diets were fed twice daily for ad libitum intake, and PG was fed in equal amounts as a top dress. All measurements were conducted at 3 time intervals starting at d -14 ± 5, d 15 and d 38 relative to calving. Propylene glycol had no effect (P > 0.05) on whole-body N balance, urinary 3-MH excretion, and body composition. However, N balance was lower (P < 0.001) at d 15 and d 38, compared to d -14. Urinary excretion of 3-MH was lower at d -14 than at d 15 (P = 0.01) and d 38 (P = 0.001). Supplemental PG had no effect (P > 0.05) on body weight (BW), and all components of empty BW. On average, cows fed both diets mobilized 19 kg of body fat and 14 kg of body protein between d -14 and d 38. Supplemental PG had no effect on mRNA abundance in skeletal muscle for m-calpain (P = 0.96) and 14-kDa ubiquitin-carrier protein E2 (14-kDa E2) (P = 0.54); however, PG supplementation down-regulated mRNA expression for µ-calpain at d 15 (P = 0.02), and tended to down-regulate mRNA expression for ubiquitin at d 15 (P = 0.07) and proteasome 26S subunit-ATPase at d 38 (P = 0.097). Relative to calving, mRNA abundance for m-calpain (P = 0.02) and µ-calpain (P = 0.005) were higher at d 15 compared to d -14 and d 38. Messenger RNA abundance for ubiquitin (P = 0.07) and 14-kDa E2 (P = 0.005) were lower at d 38 compared to d 15. In summary, these results demonstrate that up-regulation of the Ca2+-dependent and ubiquitin-mediated proteolytic pathways are the mechanisms by which skeletal muscle wasting occurs in early-lactating cows. In addition, dietary supplementation with PG may down-regulate some of these proteolytic pathways, thereby potentially attenuating undesirable skeletal muscle wasting.

The objective of this study was to determine the effects of method of barley grain processing (dry-rolled vs. pelleted barley) and source of oilseed (ground canola vs. ground flaxseed), arranged as a 2 x 2 factorial, on feed intake, ruminal fermentation, nutrient flow to the duodenum, and milk production and composition in dairy cows. Eight Holstein cows (655 ± 69 kg; 83 ± 16 DIM) were used in a replicated 4 x 4 Latin square with 28-d periods. Cows in one square were fitted with ruminal and duodenal cannulae. Cows fed dry-rolled barley consumed 1.8 to 3.5 kg/d more (P = 0.02) DM than those fed pelleted barley; however, source of supplemental dietary fat had no effect on DM intake. Ruminal pH was lower (P = 0.045) in cows fed pelleted barley compared to those fed dry-rolled barley. Ruminal concentration of acetate was greater (P = 0.001), whereas ruminal concentration of propionate tended to be lower (P = 0.11), in cows fed dry-rolled barley compared to those fed pelleted barley; consequently, the acetate:propionate ratio was higher (P = 0.01) in cows fed dry-rolled barley compared to those fed pelleted barley. Ruminal concentration of total VFA was unaffected (P > 0.05) by diet. Source of dietary fat had no effect on ruminal digestion of OM, NDF, ADF or starch; however, ruminal starch digestion was slightly higher in cows fed pelleted barley compared to those fed dry-rolled barley (90.8 vs. 89.5%). Total dietary fatty acid intake was higher (P < 0.05) in cows consuming dry-rolled barley compared to those fed pelleted barley. Duodenal flow of C18:0 was lower, whereas that of C18:2n6c was higher (P < 0.05) in cows fed pelleted barley compared to those fed dry-rolled barley. Feeding flaxseed increased duodenal flows of C18:3n3, cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid compared to feeding canola. Milk yield was unaffected (P > 0.05) by diet; however, milk fat content was higher (P = 0.004) in cows fed dry-rolled barley compared to those fed pelleted barley. Milk fat content of C18:3 was higher (P = 0.005) in cows fed canola compared to those fed flax. Milk fat content of C18:3 and cis-9, trans-11 C18:2 were higher in cows fed pelleted barley compared to those fed dry-rolled barley with flax as the source of oilseed, but not with canola (interaction, P < 0.01). Milk fat content of saturated fatty acids decreased (P < 0.001) and that of polyunsaturated fatty acids increased (P = 0.003) in cows fed pelleted barley compared to those fed dry-rolled barley. In summary, milk fatty acid profiles were altered by method of grain processing and source of oilseed.

Research where personality in animals is studied is becoming more and more common. In this area there are different concepts like behavioural syndrome and animal personality and they usually talk about consistency in behaviour over time and situations. This consistent individual behaviour can be used as an indicator of how individuals can handle and adjust to new situations in the environment. Because of this, personality studies in animals are very important in animal welfare. In this study personality in dairy cattle in lose housing were studied. 28 cows of the breeds Swedish black and white cattle (SLB) and Swedish red and white cattle (SRB) was studied during three observation situations (milking, undisturbed behaviour and novel object). With the help of Principal component analysis four components that together explained 72% of the variation in data were found. The two first components could then be interpreted. The first component corresponds to the dimension extraversion in the”Big five” model that is used in personality studies in humans. The second component was the opposite of the first one, which means that it showed low activity and low sociability. This low activity could indicate fear dependent on why the cows were standing so much. T-tests only showed significant differences in the behaviour based on the age of the cows, which indicates that the personality trait becomes more typical with higher age. The conclusion is that there are individual differences in dairy cattle which could be interpreted as personality traits.

Data from 75 F2 Brahman-Hereford cows of four specific breed combinations, F2 HB (produced by F1 HB sires x F1 HB dams, where “HB” refers to cattle sired by Hereford bulls and out of Brahman cows), F2 BH (produced by F1 BH sires x F1 BH dams), HB x BH and BH x HB, were evaluated for maternal performance at the Texas A&M Research Center near McGregor. Differences between breed combinations were analyzed for calf crop born (CCB), calf crop weaned (CCW), calf survival (CS), birth weight (BW), weaning weight (WW), and cow weight at palpation (PW). The adjusted means for F2 HB, F2 BH, HB x BH, and BH x HB were 0.84 ± 0.06, 0.57 ± 0.07, 0.82 ± 0.06, and 0.62 ± 0.08, respectively, for CCW. F2 HB cows had a 0.27 ± 0.09 higher percent calf crop weaned than F2 BH cows (P < 0.01) and a 0.22 ± 0.11 higher percent calf crop weaned than BH x HB cows (P < 0.05). HB x BH cows had a 0.25 ± 0.08 higher percent calf crop weaned than F2 BH (P < 0.01) and a 0.20 ± 0.10 higher percent calf crop weaned than BH x HB cows (P < 0.05). As 6-year-olds, the adjusted means for cow weight at palpation for F2 HB, F2 BH, HB x BH, and BH x HB cows were 523.65 ± 20.49 kg, 602.61 ± 23.63 kg, 492.84 ± 16.98 kg, and 515.93 ± 22.96 kg, respectively. Averaged across all ages, HB x BH cows weighed 56.59 ± 15.29 kg less than F2 BH cows (P < 0.001) and 41.11 ± 18.92 kg less than BH x HB cows (P < 0.05). Also, F2 HB cows weighed 40.45 ± 17.68 kg less than F2 BH cows (P < 0.05). In this herd, HB-sired cows had higher reproductive efficiency than BH-sired cows. Also, HB-sired cows tended to be lighter than BH-sired cows. Although these differences existed, exact causes could not be determined primarily due to confounding between the birth year of the cow and the sire breed of the cow.