Made available in DSpace on 2016-08-17T18:39:51Z (GMT). No. of bitstreams: 1 6222.pdf: 2879821 bytes, checksum: 010a3c6abf3b0c6f2ec81ebf9ff59ef0 (MD5) Previous issue date: 2014-08-21 / Universidade Federal de Sao Carlos / Aging causes changes in several human subsystems such as the cardiovascular and immune systems. The objective of this study was to evaluate the relationship between baroreflex function through linear algorithm (cross-spectral analysis) and proinflammatory cytokines in the aging process and also assess the baroreflex sensitivity, TNF-α, IL-6 and hsCRP in different ages and in the aging process. One hundred and ten apparently healthy subjects divided into 5 groups with 22 individuals each, according to age were evaluated: 21-30 years, 30-40 years, 40-50 years, 50-60 years, 60-70 years. The experimental protocol consisted of a blood samples collection for analysis of inflammatory markers and in the same day were collected ECG, blood pressure and respiratory movements for 15 minutes in the supine position and in standing position, after the active postural change for 15 minutes. For the analysis of baroreflex sensitivity cross spectral analysis (coherence, phase and gain) was used and ELISA method for analysis of TNF-α and IL-6 was used. Data were analyzed using analysis of variance one-way ANOVA test with Tukey test post-hoc or Kruskal-Wallis oneway ANOVA with Dunn's post-hoc according to the normality of the data and the Spearman correlation test. The level of significance for the tests was 5%. The main results were: 1) reduced baroreflex sensitivity during the aging process; 2) increased levels of inflammatory markers in the aging process; 3) negative correlation between IL-6 and BF gain and phase; 4) negative relationship between hsCRP and coherence, phase and gain in BF. It can be concluded that the human natural aging causes a loss of baroreflex sensitivity and increased serum levels of inflammatory markers studied, although the decrease in baroreflex sensitivity as function of the decreasing of vagal autonomic function occurred in the 41-50 age range, and only in the next age was observed changes in inflammatory markers. / O envelhecimento causa alterações em diversos subsistemas humanos como o cardiovascular e imunológico. O objetivo deste trabalho foi avaliar a relação entre a função barorreflexa, por meio de algoritmo linear (análise espectral cruzada) e citocinas próinflamatórias no processo de envelhecimento e ainda, avaliar a sensibilidade barorreflexa, IL- 6, TNF-α e PCRus em diversas faixas etárias e no processo de envelhecimento. Foram avaliados 110 indivíduos aparentemente saudáveis divididos em 5 grupos com 22 indivíduos cada, de acordo com a faixa etária: 21-30 anos, 30-40 anos, 40-50 anos, 50-60 anos, 60-70 anos. O protocolo experimental consistiu em uma coleta de amostras de sangue para a análise dos marcadores inflamatórios e no mesmo dia foram coletados o eletrocardiograma (ECG), a pressão arterial de pulso e a respiração por 15 minutos na postura supina, logo após foi realizada a mudança postural ativa de supino para ortostatismo, permanecendo 15 minutos nesta posição a coleta de sangue. Para a análise da sensibilidade barorreflexa foi utilizada a analise espectral cruzada e o método ELISA para análise das citocinas TNF-α e IL-6. Os dados foram analisados por meio do teste de análise de variância ANOVA one-way com posthoc de Tukey ou Kruskal-Wallis ANOVA one-way com post-hoc de Dunn s de acordo com a normalidade dos dados e teste de Correlação de Spearman. O nível de significância estabelecido para os testes foi de 5%. Os principais resultados foram: 1) redução da sensibilidade barorreflexa durante o processo de envelhecimento; 2) aumento dos níveis dos marcadores inflamatórios no processo de envelhecimento; 3) relação negativa entre a IL-6 e fase e ganho em BF; 4) relação negativa entre PCRus e coerência, fase e ganho em BF. Podese concluir que o envelhecimento natural humano causa a redução da sensibilidade barorreflexa e aumento dos níveis séricos dos marcadores inflamatórios estudados, ainda a diminuição da sensibilidade barorreflexa em consequência da diminuição da função autonômica vagal correu na faixa etária 41-50 e somente na faixa etária seguinte foi observada alterações nos marcadores inflamatórios.

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Series temporais de correntes, vento (força e direção), temperatura e salinidade, com duração de 24h, obtidas em 5 estações na Baia da Ilha Grande (rj), de 3-29/01/82, foram analisadas. As medições de corrente foram feitas a5, 12 e 20m, a cada 5min e a 2m acima do fundo, a cada 12min; temperatura e salinidade em cinco profundidades a cada 3h e vento a cada 1h. Foram utilizadas análises de series temporais através dos métodos diretos (periodograma) e indireto para as estimativas espectrais alisadas, bem como as equações hidrodinâmicas na determinação dos períodos. Foram utilizados os testes: ruído branco e fisher-whittle, a análise dos espectros rotatórios e a análise cruzada entre vento e corrente, ao longo de três camadas: superfície intermediaria e de fundo. Foram determinadas oscilações de 4,8h e identificadas como seiches internos e detectadas oscilações com período de 3,4h associadas à ressonância com o vento. Em todo o sistema da baia o período de 6h mostrou-se significativo, correspondendo à oscilação natural da baia. Foram detectadas oscilações de alta freqüência 11 e 20min, 20cm/s eventualmente associadas a vórtices e meandros da corrente. Água subtropical na camada de fundo da baia / Temporal series of currents, wind, water temperature and salinity, obtained from 5 oceanographic stations located in and around Ilha Grande Bay (RJ, Brazil), from January 23 to 29, 1982, were analyzed. AlI series have 24 hours. Currents measu¬rements were dione at depths of 5, 12, 20 m (every 5 minutes) and near the bottom (every 12 minutes). Temperature and salinity data were taken from 5 depths every 3 hours for each, while the wind was measured every hour. The present work was per¬formed in order to investigate short-period oscillations present in the region, related to density stratification and to the features of the basin, as well as to describe the circulation patterns and currents variations during one day interval. Two kinds of analysis were used: temporal series analysis, using statistical and spectral methods, and calculations by hydrodynamic motion equations. In temporal series analysis the direct (periodogram) and indirect methods were used for the spectral estimations. White-noise, Fisher- Whittle tests and rotational spectra analysis were also applied, besides the cross spectrum analysis for the data about winds and currents. The results indicate an intense and unidirectional circulation in the layer above 5 meters, from the western to the eastern portion of the Bay, through Central Channel. In this layer, on the eastern sea-opening side, a flux toward the Bay was observed. These motions, within 5 meters depth, were attributed to a circulation generated by a horizontal density difference between oceanic and inshore waters, with few contribution from tides and winds. At intermediate and near bottom layers, a horizontal homogeneity was observed and, consequent1y, smaller horizontal density gradients were detected. This way, circulation caused by tides was stronger than the movements forced by su¬perficial layers. Resultant bottom currents, observed at the Bay openings, led to the open-sea. At Central Channel, 4.8 h period oscillations were detected, and identified as internal seiches. In the eastern Portion, 3.4 hour period oscillations were detected, related to wind resonance. In alI the bay-system, the 6.0 hour period was significant, corresponding to the bay\'s natural oscillation. The study area presented a density stratification in two layers, with a single water type filling alI the portion below the 20 meters. This water, with characteristics of Subtropical Water, occupied the bottom layer due to an upwelling process produced by the dynamics of currents and winds in the continental shelf . In the two stations located off the bay, the wind and currents observations support this upwelling hypothesis.

Series temporais de correntes, vento (força e direção), temperatura e salinidade, com duração de 24h, obtidas em 5 estações na Baia da Ilha Grande (rj), de 3-29/01/82, foram analisadas. As medições de corrente foram feitas a5, 12 e 20m, a cada 5min e a 2m acima do fundo, a cada 12min; temperatura e salinidade em cinco profundidades a cada 3h e vento a cada 1h. Foram utilizadas análises de series temporais através dos métodos diretos (periodograma) e indireto para as estimativas espectrais alisadas, bem como as equações hidrodinâmicas na determinação dos períodos. Foram utilizados os testes: ruído branco e fisher-whittle, a análise dos espectros rotatórios e a análise cruzada entre vento e corrente, ao longo de três camadas: superfície intermediaria e de fundo. Foram determinadas oscilações de 4,8h e identificadas como seiches internos e detectadas oscilações com período de 3,4h associadas à ressonância com o vento. Em todo o sistema da baia o período de 6h mostrou-se significativo, correspondendo à oscilação natural da baia. Foram detectadas oscilações de alta freqüência 11 e 20min, 20cm/s eventualmente associadas a vórtices e meandros da corrente. Água subtropical na camada de fundo da baia / Temporal series of currents, wind, water temperature and salinity, obtained from 5 oceanographic stations located in and around Ilha Grande Bay (RJ, Brazil), from January 23 to 29, 1982, were analyzed. AlI series have 24 hours. Currents measu¬rements were dione at depths of 5, 12, 20 m (every 5 minutes) and near the bottom (every 12 minutes). Temperature and salinity data were taken from 5 depths every 3 hours for each, while the wind was measured every hour. The present work was per¬formed in order to investigate short-period oscillations present in the region, related to density stratification and to the features of the basin, as well as to describe the circulation patterns and currents variations during one day interval. Two kinds of analysis were used: temporal series analysis, using statistical and spectral methods, and calculations by hydrodynamic motion equations. In temporal series analysis the direct (periodogram) and indirect methods were used for the spectral estimations. White-noise, Fisher- Whittle tests and rotational spectra analysis were also applied, besides the cross spectrum analysis for the data about winds and currents. The results indicate an intense and unidirectional circulation in the layer above 5 meters, from the western to the eastern portion of the Bay, through Central Channel. In this layer, on the eastern sea-opening side, a flux toward the Bay was observed. These motions, within 5 meters depth, were attributed to a circulation generated by a horizontal density difference between oceanic and inshore waters, with few contribution from tides and winds. At intermediate and near bottom layers, a horizontal homogeneity was observed and, consequent1y, smaller horizontal density gradients were detected. This way, circulation caused by tides was stronger than the movements forced by su¬perficial layers. Resultant bottom currents, observed at the Bay openings, led to the open-sea. At Central Channel, 4.8 h period oscillations were detected, and identified as internal seiches. In the eastern Portion, 3.4 hour period oscillations were detected, related to wind resonance. In alI the bay-system, the 6.0 hour period was significant, corresponding to the bay\'s natural oscillation. The study area presented a density stratification in two layers, with a single water type filling alI the portion below the 20 meters. This water, with characteristics of Subtropical Water, occupied the bottom layer due to an upwelling process produced by the dynamics of currents and winds in the continental shelf . In the two stations located off the bay, the wind and currents observations support this upwelling hypothesis.