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The effects of whole body immersion in cold water upon subsequent terrestrial aerobic performance : a study in hypothermiaManley, Elizabeth 04 September 2013 (has links)
This study examined the extent to which physiological and psychological concomitants of aerobic terrestrial performance were affected by body cooling of varying degrees induced by cold water immersion (CWI). Thirteen male and 13 female subjects underwent three randomly assigned 30 min treadmill runs: a control run without prior manipulation of the subjects' thermal status and the same exercise after "central" (core temperature 1°C below pre-immersion) and "peripheral" cooling (skin heat loss 100kcal.m⁻².h⁻¹). During treadmill runs core temperature was measured, together with chest, leg, arm and hand temperatures, from which mean skin temperature (T [subscript]s[subscript]k) and mean body temperature (T[subscript]b) were calculated. Heart rate, oxygen consumption (VO₂,), carbon dioxide production (VCO₂), minute ventilation (V₂ (BTPS)), breathing frequency (f), cadence and ratings of perceived exertion (RPE) and thermal sensation (PTS) were also measured. Both central and peripheral cooling resulted in significantly reduced T[subscript]r[subscript]e (males : control 37.9±0. 3°C; central cooling : 36.8±0.5°C; peripheral cooling: 37.5±0.4°C; females: control: 37.9±0.4°C; central cooling: 37.2±0.5; p<0.05) during subsequent treadmill running, except following peripheral cooling for females (37.9±0.3°C) . For males and females T[subscript]s[subscript]k was lower following peripheral cooling than control values and lowest after central cooling (males: control: 30.0±1.3°C; central cooling: 36.8±0.5°C; peripheral cooling: 37.5±0.4°C; females: control: 30.5±1.2°C; central cooling: 25.9±1.8°C; peripheral cooling: 26.9±1.9°C; p<0.05). Female subjects experienced significantly higher T[subscript]r[subscript]e than males following central and peripheral cooling and a lower T[subscript]s[subscript]k following central cooling. Females experienced less of an increase in heart rate than males during exercise following central and peripheral cooling (control: l57.7±23.7b.min⁻¹; central cooling: 143.5±20.5b.min⁻¹; peripheral cooling 151.7±16.7b.min⁻¹; p<0 .05). Male responses were the same following central cooling but higher for peripheral cooling than control values (control: 139.1±7.3b.min⁻¹; central cooling 134.7±17.5b.min⁻¹; peripheral cooling: 145.0±16.4b.min⁻¹; p<0.05). These data indicate a depression in cardiovascular function for females following peripheral cooling that was not apparent for males. The VO₂ was not different between tests for males; only peripheral cooling resulted in a raised VO₂ of 28.6±3 .3ml.kg⁻¹.min⁻¹ (p<0 .05) for females compared to 27.6±2.6ml.kg⁻¹.min⁻¹ (control). A biphasic response was evident for VO₂ VCO₂ and V[subscript]B (BTPS). For both sexes overall RPE was lower for peripheral cooling (males: 9.4±1.9; females: 8.7±1.3; p<0 .05) than for control and central cooling. Central RPE was only changed for females following peripheral cooling. Changes in cadence and step length together with the effect of low skin and leg temperatures resulted in higher local RPE for females after central cooling (9.6±1.2; p<0.05) than control (9.4±1.9) and peripheral cooling (8.9±1.2 ). Males and females rated the same ambient temperature during the same exercise lower after peripheral cooling (males: 4.6±1.5; females : 5.3±1.3) than control values and lower still after central cooling (males: 3. 8±1.8; females: 2 .7±l. 5) In this study T[subscript]s[subscript]k was the primary determinant of PTS after precooling. / KMBT_363 / Adobe Acrobat 9.54 Paper Capture Plug-in
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Kinetics of water transport in biomaterials during freezing.Levin, Ronald Louis January 1976 (has links)
Thesis. 1976. Sc.D.--Massachusetts Institute of Technology. Dept. of Mechanical Engineering. / Microfiche copy available in Archives and Engineering. / Vita. / Includes bibliographical references. / Sc.D.
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In vivo and in vitro rapid cold-hardening in the Antarctic midge, Belgica antarctica: Evidence of a role for calciumTeets, Nick M. 02 May 2007 (has links)
No description available.
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Development and evaluation of cryopreservation techniques for bovine embryosPrather, Randall Scott. January 1984 (has links)
Call number: LD2668 .T4 1984 P72 / Master of Science
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Investigation of cryopreservation methods for adherent nerve cell networks in vitro.Webb, Veronica Fine 12 1900 (has links)
Cryopreservation in suspension is commonplace for a variety of cell types.
However, cryopreservation of adherent cells has achieved limited success. This research aimed to cryopreserve adherent nerve cell networks in vitro in a manner that preserved network morphology and physiology. Successful implementation would enable long term storage of adherent neuronal networks on microelectrode arrays and on-demand access for use in pharmacological and toxicological testing. Based upon morphological assessments, excellent post-thaw preservation was obtained and post-thaw cultures survived in a transitional medium for up to 3.5 hours. However, transitions to native culture medium post-thaw presented difficulties, ultimately resulting in necrosis. A discussion of methods to supplement the current research and increase post-thaw viability is included in the thesis.
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An analysis of the heat and mass transport during the freezing of biomaterials.O'Callaghan, Michael Gregory January 1979 (has links)
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering ,1979. / MICROFICHE COPY AVAILABLE IN ARCHIVES AND ENGINEERING. / Vita. / Includes bibliographical references. / Ph.D.
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Johnson-Mehl-Avrami Kinetics of Intracellular Ice Formation in Confluent Tissue ConstructsSumpter, Megan Louise 06 May 2004 (has links)
In an effort to minimize the harmful effects of intracellular ice formation (IIF) during cryopreservation of confluent tissues, computer simulations based on Monte Carlo methods were performed to predict the probability of IIF in confluent monolayers during various freezing procedures. To overcome the prohibitive computational costs of such simulations for large tissues, the well-known Johnson-Mehl-Avrami (JMA) model of crystallization kinetics was implemented as a continuum approximation of IIF in tissues. This model, which describes nucleation, growth, and impingement of crystals in a supercooled melt, is analogous to the process of intracellular ice formation and propagation in biological tissues. Based on the work of Weinberg and Kapral (1989), the JMA model was modified to account for finite-size effects, and was shown to predict accurately the results of freezing simulations in 1-D tissue constructs, for various propagation rates and tissue sizes. An initial analysis of IIF kinetics in 2-D tissues is also presented. The probability of IIF in 2-D liver tissue was measured experimentally during freezing of HepG2 cells cultured in monolayers, and compared to Monte Carlo simulations and predictions of the continuum model. The Avrami coefficient and exponent for IIF in HepG2 tissue were estimated to be k = 0.19 and n = 0.45.
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The application of the multisolute osmotic virial equation to cryobiologyPrickett, Richelle Catherine 06 1900 (has links)
Mathematical modelling of cellular osmotic responses to low temperatures is being increasingly used to overcome obstacles in the successful cryopreservation of cells and tissues. Current cryobiological models often contain simplifying assumptions regarding the solution behaviour of the complicated, multisolute intra- and extra-cellular solutions. In order to obtain more accurate predictions of cryobiological outcomes, equations derived from thermodynamic principles that more accurately describe the biological solution behaviour could be used to greatly advance the design of novel cryopreservation protocols.
The general hypothesis of this thesis is that the application of the multisolute osmotic virial equation, with mixing rules derived from thermodynamic first principles, to solutions of interest in cryobiology will result in more accurate predictions of the multisolute solution behaviour, which will lead to improved cryobiological modelling and increased understanding of cellular responses to cryopreservation.
Specifically, this thesis demonstrates that the osmotic virial coefficients, obtained from single-solute solution data, can be used in the multisolute osmotic virial equation to accurately predict the multisolute solution behaviour, without the need to fit multisolute solution data. The form of the multisolute osmotic virial equation proposed in this thesis was used to predict the solution behaviour of a range of multisolute solutions of interest in cryobiology.
The equation commonly used in cryobiology to describe cellular osmotic equilibrium is based on ideal, dilute solution assumptions. In this thesis, a non-ideal osmotic equilibrium equation was derived and, combined with the multisolute osmotic virial equation, used to more accurately predict the osmotic equilibrium of human erythrocytes.
The improved equations proposed in this thesis were combined with experimental measurements of the incidence of intracellular ice formation in order to further the understanding of the role of several important cryobiological parameters on the formation of intracellular ice.
This thesis work has significantly contributed to the field of cryobiology by substantially improving the accuracy of two key equations used in the modelling of cellular osmotic responses to cryopreservation. The combination of accurate mathematical modelling and results from experiments will allow increased understanding of cellular responses to cryopreservation, leading to the design of novel cryopreservation protocols. / Chemical Engineering and Medical Sciences
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The application of the multisolute osmotic virial equation to cryobiologyPrickett, Richelle Catherine Unknown Date
No description available.
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Finite-Difference Model of Cell Dehydration During CryopreservationCarnevale, Kevin A. 30 April 2004 (has links)
A numerical model for describing the kinetics of intracellular water transport during cryopreservation was developed. As ice is formed outside the cell, depleting the extracellular liquid of water, the cell will experience an osmotic pressure difference across its membrane, which causes cell dehydration and concomitant shrinkage. Although Mazur (1963) has previously modeled this phenomenon as a two-compartment system with membrane limited transport, the assumption of well-mixed compartments breaks down at large Biot numbers. Therefore, we have developed a numerical solution to this moving-boundary problem, including diffusive transport in the intracellular liquid, in addition to the osmotically driven membrane flux. Our model uses a modified Crank-Nicolson scheme with a non-uniform Eulerian-Lagrangian grid, and is able to reproduce predictions from Mazurs model at low Biot numbers, while generating novel predictions at high Biot numbers. Given that cell damage may result from excessive water loss, our model can be used to predict freezing methods that minimize the probability of cell injury during the cryopreservation process.
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