Thesis (doctoral)--Zhongguo ke xue yuan, Kunming zhi wu yan jiu suo and Zhongguo ke xue yuan, Zhi wu yan jiu suo, 1993. / Title on added t.p.: Systematics and evolution of Hemsleya (Cucurbitaceae). In Chinese and summary in English. Description based on print version record. Includes bibliographical references (p. 110-116) and index.

No description available.

No description available.

Thesis (Ph. D.)--University of Wisconsin-Madison. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

A asma é uma doença crônica das vias aéreas responsável por significativa morbidade e mortalidade mundial. Medicamentos anti-inflamatórios, tais como os corticosteróides, são algumas das opções de tratamento mais importantes, no entanto, elas podem causar efeitos secundários indesejáveis. Historicamente, as plantas foram uma fonte principal de moléculas com atividade biológica. O objetivo deste estudo foi avaliar o efeito do MC, um extrato vegetal brasileira com propriedades anti-inflamatórias, no tratamento da asma em um modelo animal. Métodos: Camundongos machos Balb/C, com idade de 5 a 6 semanas, foram sensibilizados com ovalbumina (OVA) por via intraperitoneal (IP) por duas vezes, com uma semana de intervalo, e desafiados diariamente com OVA intranasal durante três dias. Os animais foram tratados diariamente com a MCA (aquoso) MCHA (hidroalcóolico) na dose 500 mg/kg ip por três dias, durante os desafios. Os camundongos do grupo controle receberam solução salina nos mesmos dias. Cinco a oito animais por grupo foram utilizados. Vinte e quatro horas após o último desafio, os ratinhos foram ventilados com um respirador animal pequeno (FlexiVent®), e foram realizadas medições in vivo da hiperreactividade brônquica com concentrações crescentes de metacolina em aerossol (6,25, 12,5, 25 e 50 mg / ml). Os parâmetros avaliados e comparados foram: a resistência total (RRS), elastância total (ERS), resistência do tecido (G) e elastância tecidual (H). Após ventilação, foi colhido lavado broncoalveolar (LBA) para análise da contagem de células totais e diferenciais. Interleucinas inflamatórias (IL-4, IL-5, IL-10, IL- 13, IFN-?) foram analisadas no homogenato pulmonar além da dosagem sérica de IgE antiOVA. Os pulmões dos animais foram coletados para análise histológica (H&E). Resultados: O tratamento com extrato MCHA reduziu significativamente a hiperresponsividade brônquica através da mensuração das medidas RRS (p<0,001), ERS (p<0,05), G (p<0,05) and H (p<0,001), quando comparado com o grupo de animas asmáticos não tratados. Extratos de MCA e MCHA reduziram tambem significativamente células totais (p<0,05) e contagem de eosinófilos (p<0,05). MCHA reduziu a concentração de IFN-? (p<0,01) quando comparado aos animais asmáticos não tratados. MCA (p<0,001) mostrou uma significativa redução do número de células inflamatórias por área quando comparado ao grupo asmático não tratado. Conclusão: Os dois extratos (aquoso e hidroetanólico) das folhas da espécie Momordica charantia L., na dose de 500 mg/kg, foi eficaz no tratamento da asma em modelo animal induzido por ovalbumina tanto em medidas da mecânica pulmonar quando em marcadores inflamatórios e estudo histológico, sendo o extrato hidroetanólico potencialmente mais eficaz na dose estudada. / Asthma is a chronic disease of the airways responsible for significant morbidity and mortality worldwide. Anti-inflammatory medications, such as corticosteroids, are some of the most important treatment options, however they can cause undesirable side effects. Historically, plants have been a major source of molecules with biological activity. The objective of this study was to evaluate the effect of MC, a Brazilian herbal extract with anti-inflammatory properties, on asthma treatment in an animal model. Methods: Male Balb/c mice, 5 to 6 weeks, were sensitized twice with ovalbumin (OVA) intraperitoneally (ip), one week apart, and challenged daily with OVA intranasally for three days. Mice were treated daily with MCA (aqueous) MCHA (hydroethanolic) extract (500 mg/kg) ip for three days, during challenges. Control mice received saline on the same days. Five to eight mice were utilized per group. Twenty-four hours after the last challenge, mice were ventilated with a small-animal ventilator (FlexiVent®), and in vivo measurements of bronchial hyperresponsiveness were performed with increasing concentrations of methacholine aerosol (6.25, 12.5, 25 and 50 mg/ml). The following parameters were evaluated and compared: total resistance (RRS), total elastance (ERS),, tissue resistance (G) and tissue elastance (H). After ventilation, was collected bronchoalveolar lavage (BAL) for analysis of total and differential count inflammatory cells. Interleukins (IL-4, IL-5, IL-10, IL-13, IFN-?) were analyzed in lung homogenate. Morover serum anti-OVA IgE were dosage. The lungs of the animals were collected for histological analysis (H & E) Results: Treatment with MCHA extract significantly decreased airway hyperresponsiveness, measured by RRS (p<0,001), ERS (p<0,05), G (p<0,05) and H (p<0,001), when compared to OVA-challenged mice. MCA e MCHA extract also significantly reduced BAL total cell (p<0,05) and eosinophil counts (p<0,05). MCHA reduced IFN-? concentration (p<0,01) as compared to the untreated group asthmatic. MCA (p<0,001) showed a significant reduction in the number of inflammatory cells per unit area in the air compared to the asthmatic untreated group. Conclusion: The administration of MCA and MCHA from the leaves of Momordica charantia L. species, at a dose of 500 mg / kg was effective in the treatment of asthma in animal models induced by ovalbumin in both pulmonary mechanics measurements as inflammatory markers, and histological study, and potentially more effective MCHA extract the studied dose.

A asma é uma doença crônica das vias aéreas responsável por significativa morbidade e mortalidade mundial. Medicamentos anti-inflamatórios, tais como os corticosteróides, são algumas das opções de tratamento mais importantes, no entanto, elas podem causar efeitos secundários indesejáveis. Historicamente, as plantas foram uma fonte principal de moléculas com atividade biológica. O objetivo deste estudo foi avaliar o efeito do MC, um extrato vegetal brasileira com propriedades anti-inflamatórias, no tratamento da asma em um modelo animal. Métodos: Camundongos machos Balb/C, com idade de 5 a 6 semanas, foram sensibilizados com ovalbumina (OVA) por via intraperitoneal (IP) por duas vezes, com uma semana de intervalo, e desafiados diariamente com OVA intranasal durante três dias. Os animais foram tratados diariamente com a MCA (aquoso) MCHA (hidroalcóolico) na dose 500 mg/kg ip por três dias, durante os desafios. Os camundongos do grupo controle receberam solução salina nos mesmos dias. Cinco a oito animais por grupo foram utilizados. Vinte e quatro horas após o último desafio, os ratinhos foram ventilados com um respirador animal pequeno (FlexiVent®), e foram realizadas medições in vivo da hiperreactividade brônquica com concentrações crescentes de metacolina em aerossol (6,25, 12,5, 25 e 50 mg / ml). Os parâmetros avaliados e comparados foram: a resistência total (RRS), elastância total (ERS), resistência do tecido (G) e elastância tecidual (H). Após ventilação, foi colhido lavado broncoalveolar (LBA) para análise da contagem de células totais e diferenciais. Interleucinas inflamatórias (IL-4, IL-5, IL-10, IL- 13, IFN-?) foram analisadas no homogenato pulmonar além da dosagem sérica de IgE antiOVA. Os pulmões dos animais foram coletados para análise histológica (H&E). Resultados: O tratamento com extrato MCHA reduziu significativamente a hiperresponsividade brônquica através da mensuração das medidas RRS (p<0,001), ERS (p<0,05), G (p<0,05) and H (p<0,001), quando comparado com o grupo de animas asmáticos não tratados. Extratos de MCA e MCHA reduziram tambem significativamente células totais (p<0,05) e contagem de eosinófilos (p<0,05). MCHA reduziu a concentração de IFN-? (p<0,01) quando comparado aos animais asmáticos não tratados. MCA (p<0,001) mostrou uma significativa redução do número de células inflamatórias por área quando comparado ao grupo asmático não tratado. Conclusão: Os dois extratos (aquoso e hidroetanólico) das folhas da espécie Momordica charantia L., na dose de 500 mg/kg, foi eficaz no tratamento da asma em modelo animal induzido por ovalbumina tanto em medidas da mecânica pulmonar quando em marcadores inflamatórios e estudo histológico, sendo o extrato hidroetanólico potencialmente mais eficaz na dose estudada. / Asthma is a chronic disease of the airways responsible for significant morbidity and mortality worldwide. Anti-inflammatory medications, such as corticosteroids, are some of the most important treatment options, however they can cause undesirable side effects. Historically, plants have been a major source of molecules with biological activity. The objective of this study was to evaluate the effect of MC, a Brazilian herbal extract with anti-inflammatory properties, on asthma treatment in an animal model. Methods: Male Balb/c mice, 5 to 6 weeks, were sensitized twice with ovalbumin (OVA) intraperitoneally (ip), one week apart, and challenged daily with OVA intranasally for three days. Mice were treated daily with MCA (aqueous) MCHA (hydroethanolic) extract (500 mg/kg) ip for three days, during challenges. Control mice received saline on the same days. Five to eight mice were utilized per group. Twenty-four hours after the last challenge, mice were ventilated with a small-animal ventilator (FlexiVent®), and in vivo measurements of bronchial hyperresponsiveness were performed with increasing concentrations of methacholine aerosol (6.25, 12.5, 25 and 50 mg/ml). The following parameters were evaluated and compared: total resistance (RRS), total elastance (ERS),, tissue resistance (G) and tissue elastance (H). After ventilation, was collected bronchoalveolar lavage (BAL) for analysis of total and differential count inflammatory cells. Interleukins (IL-4, IL-5, IL-10, IL-13, IFN-?) were analyzed in lung homogenate. Morover serum anti-OVA IgE were dosage. The lungs of the animals were collected for histological analysis (H & E) Results: Treatment with MCHA extract significantly decreased airway hyperresponsiveness, measured by RRS (p<0,001), ERS (p<0,05), G (p<0,05) and H (p<0,001), when compared to OVA-challenged mice. MCA e MCHA extract also significantly reduced BAL total cell (p<0,05) and eosinophil counts (p<0,05). MCHA reduced IFN-? concentration (p<0,01) as compared to the untreated group asthmatic. MCA (p<0,001) showed a significant reduction in the number of inflammatory cells per unit area in the air compared to the asthmatic untreated group. Conclusion: The administration of MCA and MCHA from the leaves of Momordica charantia L. species, at a dose of 500 mg / kg was effective in the treatment of asthma in animal models induced by ovalbumin in both pulmonary mechanics measurements as inflammatory markers, and histological study, and potentially more effective MCHA extract the studied dose.

Thesis (M.Sc.(Hons.)) -- University of Western Sydney, 2007. / A thesis submitted in fulfilment of the requirements for the degree of Master of Science (Hons) to the University of Western Sydney, College of Health and Science, Centre for Plant and Food Science. Includes bibliography.

Call number: LD2668 .T4 1960 M87

Salt-soluble proteins of Apodanthera undulata, Cucurbita digitata, C. foetidissima, C. moschata, C. palmeri and C. sororia, were isolated from the decorticated seeds. The isoelectric focusing pattern of A. undulata was divided into two groups, the first falling between pH 4.43 to 5.48 and consisting of six distinct peaks. The pattern of C. digitata consisted of three groups of six, two and three peaks falling in the pH ranges of 5.19 to 6.14, 6.48 to 7.00 and 7.14 to 7.89, respectively. The pattern for C. foetidissima was divided into three groups of four, two and three peaks which fell in the pH ranges 4.82 to 5.82, 6.38 to 6.86 and 7.00 to 8.00, respectively. The patterns of C. palmeri and C. sororia were virtually identical, with two groups of eight and five peaks which fell in the pH range 4.54 to 5.72 and 6.24 to 7.77, respectively. The C. moschata pattern showed three groups of seven, seven and five peaks in the pH range 5.00 to 5.91, 6.00 to 7.00 and 7.24 to 8.29, respectively. On electrophoresis the globulins of all five Cucurbita species displayed six distinct bands with the same relative mobilities, while the globulin pattern of A. undulata was quite different. There was no similarity among the patterns of the Cucurbita albumins, and none resembled that of A. undulata. Two-dimensional analysis of the salt-soluble extracts showed similarity among the five Cucurbita species and differences from A. undulata with respect to the relative mobilities of the resolved bands. Molecular weight determination by gel filtration, resulted in resolution of each globulin fraction into four peaks except that of C. moschata, which was resolved into five peaks. Molecular weight values ranged from 12,000 to 660,000. Each albumin fraction was resolved into three peaks except that of A. undulata which possessed four peaks. The molecular weight values ranged from 11,000 to 446,000.

Thesis (doctoral)--University of Chicago, 1930. / "Part of a dissertation ..." Includes bibliographical references (p. 31-32).