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M.Tech. / In this age of ever-increasing competition, organisations are facing unprecedented pressure to meet the combined obligations of showing returns to shareholders, and staying ahead of the competition. To meet these obligations, organisations have become increasingly dependent on technology, as an enabler. This dependency suggests that technology projects have become strategically more important than ever for organisations; yet the success of technology projects remains questionable. Furthermore, organisations do not have simple mechanisms to allow them to quickly and accurately trace the causes of IT project management failures. One of the causes of project management failures is the inability and/or unwillingness of project managers to adhere to project management best practices adopted by their organisations. This research proposes a simple and repeatable model to help organisations determine whether they are indeed following the project management best practices which they purport to follow. The research methods consisted firstly of a wide review of relevant literature on auditing, project management, and IT governance. Secondly, empirical data was collected and analysed. Thirdly, modelling was used to develop a conceptual model for auditing IT project management. The empirical study is based on a semi-structured interview, involving ten project managers in charge of IT projects. The findings from this research confirm that project managers do not adhere to project management best practices which they purport to follow. Consequently, this dissertation concludes that IT project managers must adhere to best practices adopted by their organisations, regardless of how impractical or inconvenient that may seem; the proposed model for auditing IT project management helps them achieve just that.

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M.Tech. (Operations Management) / The South African manufacturing sector is considered the main driver of economic growth, but is falling behind in adopting and adequately using information and communication technology (ICT) to improve productivity and competitiveness. This is creating a constraint that hinders the manufacturing sector, adversely affecting its ability to access the global market and produce high value-added goods, and also to compete globally. Thus, the main objective of this dissertation is to gain an in-depth understanding of the impact of ICT on service design and operations to address barriers to manufacturing sector growth and to improve productivity and competitiveness. This study proposes an ICT-based service design model to increase productivity and competitiveness in the South African manufacturing sectors. It argues that the challenges faced can be addressed and that some of the solutions lie in the integration of ICT into service design and operations processes. The research adopted an interpretive approach through qualitative methods and, was carried out as a single case study using ArcelorMittal South Africa for which a purposive sampling technique was considered appropriate. Qualitative data were collected through semi-structured interviews, and tape recorded and analysed through descriptive procedures. The findings suggest that ICT diffuses rapidly, creating opportunities and challenges for manufacturing companies. In addition it was found out that the adoption and adequate use of ICT depends on the implementation phase. This is when most challenges and impacts are experienced: for example, technological capability, technical skills, resources and ICT infrastructure capabilities. In addition, the findings suggest that a more educated workforce possesses the capability to acquire the necessary skills, in the form of self-education to overcome any challenges encountered. Evidence also shows that companies are facing challenges in relation to the selection and integration of the right technologies due to lack of ICT expertise. Therefore, this research proposes an ICT-based service design model to increase productivity and global competitiveness in the manufacturing sectors in South Africa.

Although the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. In these proteins, selenium (Se) is incorporated in the form of selenocysteine(Sec), the 21st amino acid. Selenocysteine is cotranslationally inserted in response to UGA codons (a stop signal in the canonical genetic code). The alternative decoding is mediated by a stem-loop structure in the 3'UTR of selenoprotein mRNAs (the SECIS element). Selenium is implicated in male infertility, cancer and heart diseases, viral expression and ageing. In addition, most selenoproteins have homologues in which Sec is replaced by cysteine (Cys).Genome biologists rely on the high-quality annotation of genomes to bridge the gap from the sequence to the biology of the organism. However, for selenoproteins, which mediate the biological functions of selenium, the dual role of the UGA codon confounds both the automatic annotation pipelines and the human curators. In consequence, selenoproteins are misannotated in the majority of genome projects. Furthermore, the finding of novel selenoprotein families remains a difficult task in the newly released genome sequences.In the last few years, we have contributed to the exhaustive description of the eukaryotic selenoproteome (set of eukaryotic selenoproteins) through the development of a number of ad hoc computational tools. Our approach is based on the capacity of predicting SECIS elements, standard genes and genes with a UGA codon in-frame in one or multiple genomes. Indeed, the comparative analysis plays an essential role because 1) SECIS sequences are conserved between close species (eg. human-mouse); and 2) sequence conservation across a UGA codon between genomes at further phylogenetic distance strongly suggests a coding function (eg. human-fugu). Our analysis of the fly, human and Takifugu and Tetraodon genomes have resulted in 9 novel selenoprotein families. Therefore, 20 distinct selenoprotein families have been described in eukaryotes to date. Most of these families are widely (but not uniformly) distributed across eukaryotes, either as true selenoproteins or Cys-homologues.The correct annotation of selenoproteins is thus providing insight into the evolution of the usage of Sec. Our data indicate a discrete evolutionary distribution of selenoprotein in eukaryotes and suggest that, contrary to the prevalent thinking of an increase in the number of selenoproteins from less to more complex genomes, Sec-containing proteins scatter all along the complexity scale. We believe that the particular distribution of each family is mediated by an ongoing process of Sec/Cys interconversion, in which contingent events could play a role as important as functional constraints. The characterization of eukaryotic selenoproteins illustrates some of the most important challenges involved in the completion of the gene annotation of genomes. Notably among them, the increasing number of exceptions to our standard theory of the eukaryotic gene and the necessity of sequencing genomes at different evolutionary distances towards such a complete annotation.