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Microbiology and the limits to life in deep saltsPayler, Samuel Joseph January 2018 (has links)
Deep subsurface evaporites are common terrestrial deep subsurface environments found globally. These deposits are known to host communities of halophilic organisms, some of which have been suggested to be millions of years old. The discovery of evaporite minerals on Mars has led to these environments becoming of interest to astrobiology, particularly because the subsurface of Mars represents the best chance of finding more clement conditions conducive to life. Despite this interest, deep subsurface evaporites remain poorly understood and we have little insight into how different salts shape the Earth's biosphere, much of which is underground. This thesis addresses several knowledge gaps present in the literature by sampling a selection of brine seeps and rock salt samples taken from Boulby Potash Mine, UK. The origin and evolution of the brines is determined with geochemical techniques, showing the majority to have been sourced from an aquifer above where they were intersected in the mine. These brines appear to have taken a variety of pathways through the subsurface leading to the presence of a range of different ions dissolved within them. The majority are Na/Cl dominated, whilst one is K/Cl dominated. One brine appears to have a different origin and probably interacted with dolomite becoming very concentrated in Mg. This variety in brine origins and migration pathways has impacted the habitability of the brines. Physicochemical measurements for chaotropicity, water activity and ionic strength, combined with culturing experiments suggest brines from the Sherwood Sandstone were habitable, but the brine from a distinct unknown source was uninhabitable. DNA was successfully extracted from three of the habitable brines and their metagenomes sequenced. These revealed communities largely functionally and phylogenetically similar to surface near saturation brines, indicating that the structure of the communities present in saturated Na/Cl brines are controlled almost exclusively by these ions rather than any other environmental difference between the surface and subsurface. Organisms were also taken from these brines and culturing experiments carried out to determine if any carbon sources were present in ancient salt that might promote growth in the absence of other carbon sources. Controls showed that the geochemical changes to the growth media induced by solving the salts, particularly sylvinite, were responsible for the increases in growth observed, indicating certain salt minerals effectively fertilise the growth of halophiles. Culturing on hydrocarbon seeps collected in the mine suggested they may provide a carbon source periodically to some organisms within the deposit. Work was done to show the presence of dissimilatory sulphate and iron reducing halophiles. Overall this significantly advances our understanding of how salts shape the Earth's biosphere, particularly its deep subsurface component, and what functional capabilities life has to persist in these environments. This work provides a new window on the potential habitability of deep subsurface extraterrestrial environments and how we might go about investigating these environments for habitable conditions.
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Structure and function of microbial communities in acid sulfate soil and the terrestrial deep biosphereWu, Xiaofen January 2016 (has links)
This thesis describes the use of different DNA sequencing technologies to investigate the structure and function of microbial communities in two extreme environments, boreal acid sulfate soil and the terrestrial deep biosphere. The first of the two investigated environments was soils containing un-oxidized metal sulfides that are termed ‘potential acid sulfate soil’ (PASS) materials. If these materials are exposed to atmospheric oxygen by either natural phenomena (e.g., land uplift) or human activities (e.g., drainage) then the metal sulfides become oxidized and the PASS becomes acidic and is defined as an ‘acid sulfate soil’ (ASS). The resulting acid and metal release from metal sulfide oxidation can lead to severe environmental damage. Although acidophilic microorganisms capable of catalyzing acid and metal release have been identified from many sulfide mineral containing environments, the microbial community of boreal PASSs/ASSs remains unclear. This study investigated the physicochemical and microbial characteristics of PASSs and ASSs from the Risöfladan experimental field in Vasa, Finland. Sanger sequencing of 16S rRNA gene sequences of microorganisms present in the PASSs and ASSs were mostly assigned to acidophilic species and environmental clones previously identified from acid- and metal-contaminated environments. Enrichment cultures inoculated from the ASS demonstrated that the acidophilic microorganisms were responsible for catalyzing acid and metal release from PASSs/ASSs. Lastly, the study investigated how to mitigate metal sulfide oxidation and the concomitant formation of sulfuric acid by treating ASSs in situ with CaCO3 or Ca(OH)2 suspensions. The DNA sequencing still identified acidophilic microorganisms after the chemical treatments. However, the increased pH during and after treatment suggested that the activity of the acidophiles might be inhibited. This study was the first to identify the microbial community present in boreal PASSs/ASSs and suggested that treatment with basic compounds may inhibit microbial catalysis of metal sulfide dissolution. The second studied environment was the deep, dark terrestrial subsurface that is suggested to be both extremely stable and highly oligotrophic. Despite the scarcity of carbon and energy sources, the deep biosphere is estimated to constitute up to 20% of the total biomass on earth and thus, represents the largest microbial ecosystem. However, due to the difficulties of accessing this environment and our inability to cultivate the indigenous microbial populations, details of the diversity and metabolism of these communities remain largely unexplored. This study was carried out at Äspö Hard Rock Laboratory, Sweden and utilized second-generation sequencing to identify the taxonomic composition and genetic potential of planktonic and biofilm populations. Community DNA sequencing of planktonic cells from three water types at varied age and depth (‘modern marine’, ‘undefined mixed’, and ‘old saline’) showed the existence of ultra-small cells capable of passing through a 0.22 μm filter that were phylogenetically distinct communities from the >0.22 μm fraction. The reduced cell size and/or genome size suggested a potential adaptation to the oligotrophic environment in the terrestrial deep biosphere. The identified planktonic communities were dominated by Proteobacteria, Candidate divisions, unclassified archaea, and unclassified bacteria. Functional analysis of the assembled genomes showed that the planktonic population from the shallow modern marine water demonstrated a predominantly anaerobic and heterotrophic lifestyle. In contrast, the deeper, old saline water was more closely aligned with the hypothesis of a hydrogen-driven deep biosphere. Metagenomic analysis of subsurface biofilms from ‘modern marine’ and ‘old saline’ water types suggested only a subset of populations were involved in initial biofilm formation. The identified biofilm populations from both water types were distinct from the planktonic community and were suggested to be dominated by hydrogen fed, chemolithoautotrophic and diazotrophic populations.
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Raman spectroscopy in Geobiology - Advances in detection and interpretation of organic signatures in rocks and mineralsSchäfer, Nadine 12 April 2013 (has links)
No description available.
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Mikrobielle Diversität an diffusen Quellen des Mittel-Atlantischen Rückens / Microbial diversity within the low-temperature influenced deep marine biosphere along the Mid-Atlantic-RidgeRathsack, Kristina 08 November 2010 (has links)
No description available.
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Adaptations à la vie sous haute pression hydrostatique chez les microorganismes piézophiles, l'exemple de Thermococcus barophilus / Adaptations of life under high hydrostatic pressure in piezophilic microorganisms, the exemple of Thermococcus barophilusCario, Anaïs 25 November 2013 (has links)
Les environnements profonds marins ou continentaux représentent la majorité des biotopes sur Terre. Ils sont colonisés par des organismes, appelés piézophiles, adaptés aux fortes pressions hydrostatiques du milieu, conditions qui sont inhibitrices pour la croissance des organismes de surface. Dans le cadre de ce travail, j'ai cherché à élucider les spécificités de l’adaptation aux hautes pressions hydrostatiques. Pour cela, j'ai étudié un micro-organisme piézophile issu d'une source hydrothermale profonde, la souche MP de Thermococcus barophilus, dont l'optimum de croissance est de 400 fois la pression atmosphérique. J'ai caractérisé l'adaptation particulière de deux cibles cellulaires parmi les plus sensibles à la pression : les membranes et le protéome.Mes résultats montrent que la souche MP accumule des molécules de stress en condition de faible pression hydrostatique, c'est-à-dire que le protéome de cette souche est adapté aux conditions de hautes pressions. Il s'agit de la première démonstration d'une adaptation structurale chez un piézophile, et la démonstration que cette souche est une piézophile vraie. Par ailleurs, j'ai pu démontrer les mécanismes d'adaptation de la membrane en réponse à la pression et à la température. J'ai montré que cette réponse correspond à une adaptation homéovisqueuse de la composition membranaire, et que celle-ci est unique, car elle met en jeu trois mécanismes différents : une régulation du ratio di-/tetraéthers, une régulation du niveau d'insaturation des lipides, et la présence de lipides neutres dans la structure de la membrane. Ceci m'a amenée à proposer un nouveau modèle de membrane pour la souche modèle piézophile T. barophilus. La généralisation de ces observations et la confirmation de leur lien avec la piézophilie passe par l'étude d'autres organismes piézophiles. / Deep marine and continental environments represent the major ecosystems on Earth. They are colonized by organisms named piezophiles, adapted to high pressures of the deep biosphere, conditions that inhibit the growth of surface organisms. My objectives were to elucidate the special features of adaptation to high hydrostatic pressures. My model of study was a piezophilic microorganism isolated from a deep-sea vent; Thermococcus barophilus strain MP, which grows optimally at a pressure of 400 times the atmospheric pressure. I characterized the specific adaptation of two cellular compartments amongst the most sensitive to pressure: membranes and proteome. My results show that strain MP accumulates stress molecules in conditions of low pressure, which mean T. barophilus proteome is adapted to high pressure conditions. This is the first demonstration of structural adaptation in a piezophile, and also shows that T. barophilus is a true piezophile. Besides, I proved membrane adaptation mechanisms in response to pressure and temperature. These mechanisms are based on homeoviscous adaptation of lipids composition. This adaptation is unique and involves three different mechanisms: the regulation of the di-/tetraether ratio, the modulation of lipid unsaturation, and the insertion of neutral lipids in the membrane structure. These results brought me to propose a new membrane model for the piezophilic strain T. barophilus. Before confirming these observations as a possible piezophilic trait of adaptation, this study needs to be extended to other piezophilic organisms.
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Microbiology of basalts targeted for deep geological carbon sequestration : field observations and laboratory experimentsLavalleur, Heather J. 15 June 2012 (has links)
With rising concentrations of CO₂ in the Earth's atmosphere causing
concern about climate change, many solutions are being presented to
decrease emissions. One of the proposed solutions is to sequester excess
CO₂ in geological formations such as basalt. The deep subsurface is known
to harbor much of the microbial biomass on earth and questions abound as to
how this deep life is going to respond to the injection of CO₂. Many studies
have used model microorganisms to demonstrate the ability of microbes to aid
in the safe, permanent sequestration of CO₂ in the subsurface. The objective
of this research is to characterize the microbial community present in the
basalts at the Wallula pilot carbon sequestration well prior to the injection of
CO₂ and then perform laboratory studies to determine how the native microbial
community will respond to carbon sequestration conditions. Six samples were
collected from the Wallula pilot well prior to the injection of CO₂ into the
system. The microorganisms in these samples were characterized by
pyrosequencing of 16S rRNA genes, revealing a community dominated by the
Proteobacteria, Firmicutes, and Actinobacteria. The organisms detected were
related to microbes known to metabolize hydrogen, sulfur, and single carbon
compounds. These microorganisms may be stimulated in formations located
at the fringe of the pool of injected CO₂. Laboratory studies revealed that the
native microbial community suffered a two order of magnitude loss of
population upon exposure to CO₂ under carbon sequestration conditions. The
community also shifted from being dominated by Proteobacteria prior to CO₂
exposure to being dominated by Firmicutes after exposure. Specifically, the
genus Alkaliphilus, which was previously undetected, appeared after CO₂
exposure and became dominant. The dominance of Alkaliphilus, along with
other rare organisms which did not compose a majority of the population prior
to the introduction of CO₂ to the system, indicates that members of the rare
biosphere may be better adapted to changing environmental conditions
specific to CO₂ sequestration than other indigenous cells. Thus, the rare
biosphere should be examined closely as part of any environmental study, as
these minority microorganisms may be the first indication of perturbation or
impact. / Graduation date: 2013
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An integrated approach to the study of biosignatures in mineralizing biofilms and microbial mats / Ein umfassender Ansatz zur Untersuchung von Lebensspuren in mineralisierenden Biofilmen und mikrobiellen MattenHeim, Christine Nora 09 July 2010 (has links)
No description available.
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