Two experiments explore the application of plasmonic nanoparticles to cellular pathology. The first devised a platform by which gold-silver nanoparticles act as differentiable labels for cell surface receptors under dark field imaging. By conjugating particles of various constitutions with receptor-targeting antibodies, particles scatter characteristically according to their plasmon peak. The second experiment programmed receptor placement via the patterning of two substrates and used the binding of SERS nanoparticles to explore the quantification of such targets at high-density. On one substrate, anchor pairs established receptors at specified distances in order to define the relationship between scattering intensity and the distance between SERS particles. On the second, anchor regions are filled with increasing densities of receptors and the particle-saturated substrates are probed to relate scattering intensity to particle density. This should discover the density-threshold between linear and non-linear scattering and inform the quantification of particles in the exponential density regime.

Two experiments explore the application of plasmonic nanoparticles to cellular pathology. The first devised a platform by which gold-silver nanoparticles act as differentiable labels for cell surface receptors under dark field imaging. By conjugating particles of various constitutions with receptor-targeting antibodies, particles scatter characteristically according to their plasmon peak. The second experiment programmed receptor placement via the patterning of two substrates and used the binding of SERS nanoparticles to explore the quantification of such targets at high-density. On one substrate, anchor pairs established receptors at specified distances in order to define the relationship between scattering intensity and the distance between SERS particles. On the second, anchor regions are filled with increasing densities of receptors and the particle-saturated substrates are probed to relate scattering intensity to particle density. This should discover the density-threshold between linear and non-linear scattering and inform the quantification of particles in the exponential density regime.

Text in Afrikaans / The aim of this study was to determine the effect of labeling on a person's being from an educational-psychological perspective. The focus was therefore placed mainly on the following: § Labeling as a phenomenon, with specific reference to what labeling is and its effect on a person's being. § The use of the Edu-Psychological Relation Theory as a theoretical basis for determining the effect of labeling on a person's being. § The compilation of a diagnostic questionnaire within the framework of the Edu-Psychological Relation Theory that was used in the empirical study. It was found that the consequences of labeling are generally permanent and irreversible, and that they result in isolation of the individual. A diagnostic questionnaire was operationalised in terms of the Edu-Psychological Relation Theory, and was used to determine the effect of labeling. / Educational Studies / M. Ed. (Guidance)

Research indicates that the number of individuals diagnosed with neurological, learning and psychiatric disorders has shown a sharp increase in recent years. An increasing acknowledgement of the importance of narratives and discourses in constructing social reality has stimulated much debate on the consequences of diagnosing individuals with such diagnostic labels. The aim of this study was to explore the ways in which such individuals construct meaning from their experiences of adapting to their diagnostic labels by reframing them as interpersonal metaphors. In service of this aim, a social constructionist epistemology was adopted and discourse analysis was used to analyse the results from three participants’ interview data. The results indicate that participants managed to construct meaning from their experiences with their diagnostic labels through a reframing process that serve to promote positive perceptions of self in relation to others. Furthermore, this meaning-construction process appears to be a reflective and interactional one, in that it relies on a negotiation of meanings between people in a retrospective fashion. / Psychology / M.A. (Clinical Psychology)

Text in Afrikaans / The aim of this study was to determine the effect of labeling on a person's being from an educational-psychological perspective. The focus was therefore placed mainly on the following: § Labeling as a phenomenon, with specific reference to what labeling is and its effect on a person's being. § The use of the Edu-Psychological Relation Theory as a theoretical basis for determining the effect of labeling on a person's being. § The compilation of a diagnostic questionnaire within the framework of the Edu-Psychological Relation Theory that was used in the empirical study. It was found that the consequences of labeling are generally permanent and irreversible, and that they result in isolation of the individual. A diagnostic questionnaire was operationalised in terms of the Edu-Psychological Relation Theory, and was used to determine the effect of labeling. / Educational Studies / M. Ed. (Guidance)

Research indicates that the number of individuals diagnosed with neurological, learning and psychiatric disorders has shown a sharp increase in recent years. An increasing acknowledgement of the importance of narratives and discourses in constructing social reality has stimulated much debate on the consequences of diagnosing individuals with such diagnostic labels. The aim of this study was to explore the ways in which such individuals construct meaning from their experiences of adapting to their diagnostic labels by reframing them as interpersonal metaphors. In service of this aim, a social constructionist epistemology was adopted and discourse analysis was used to analyse the results from three participants’ interview data. The results indicate that participants managed to construct meaning from their experiences with their diagnostic labels through a reframing process that serve to promote positive perceptions of self in relation to others. Furthermore, this meaning-construction process appears to be a reflective and interactional one, in that it relies on a negotiation of meanings between people in a retrospective fashion. / Psychology / M.A. (Clinical Psychology)