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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Detec??o de ?cido ocadaico em cultivo de mexilh?es Perna perna (Linn?, 1758) e identifica??o do fitopl?ncton potencialmente produtor, em Maci?is, Angra dos Reis, RJ. / Okadaic acid detection in mussel cultivation, Perna perna (Linn?, 1758), and the fitoplankton identification potencially producer in the coast area of Maci?is, Angra dos Reis, RJ.

Marin?, Geisi Ferreira 22 June 2007 (has links)
Made available in DSpace on 2016-04-28T20:17:25Z (GMT). No. of bitstreams: 1 2007-Geisi Ferreira Marine.pdf: 800208 bytes, checksum: b24185c0919f8244f06ce7f08948693e (MD5) Previous issue date: 2007-06-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The okadaic acid phycotoxin AO is produced by a group of micro seaweed known as Dinoflagellated. The mussels when feeding themselves from this micro seaweed accumulate in their hepatopancreas, this toxin, trigging in the human being the Syndrome or Diarrhetic Shellfish Poisoning (DSP). The symptoms appear at around 30 minutes after the consumption of the contaminated clam; the symptoms vary among abdominal nauseas, pains, vomits and diarrhea. When the toxin ingestion happens in amounts lower than 48 μg.g-1, the above described symptoms do not develop, however, its continued consumption favors the appearing of tumors in the gastrointestinal tract due to the high carcinogenic power of AO. This study intended to detect and quantify the diarrheic toxin AO in Perna perna mussels collected between the months of May and December of the 2006 and verification of the potentially toxic micro seaweed presence, in the seasons Spring/Summer (from September until December of 2006). Since May until december of 2006, mussels (Perna perna) were collected and analyzed regarding to the presence of the Phycotoxin Ao .In order to collect the micro seaweed were used a plankton net (20μm of mesh) and Bomb Rule 2000. The identification of the dinoflagellates ones was carried through in inverted biological microscope. The detection of AO in the mussels was carried through by High Efficiency of Liquid Chromatography with Fluorimetric Detection (HPLC-FC). The chromatographic results had indicated the presence of AO toxin in all the gotten mussel samples from May until October of 2006 in low concentrations. In the analyses of phytoplankton, the diatoms were the most representative group compared with the Dinoflagellated. The species Prorocentrum micans and P. gracile examined were not pointed as toxin producers until the moment. Among the Dino-flagellated potentially toxic were found the species : Dinophysis acuminata, D. tripos, D. rotundata and D. fortii. The results indicate the necessity of elaboration and effective application of a hygienic-sanitary controlling program of the clams as well as monitoring the environment, aiming above everything the public health Safety. / A ficotoxina ?cido ocadaico (AO) ? produzida por um grupo de microalgas conhecidas como dinoflagelados. Os mexilh?es ao se alimentarem destas microalgas acumulam em seu hepatop?ncreas, esta toxina, desencadeando no ser humano a S?ndrome ou Envenenamento Diarr?ico por Moluscos - EDM. Os sintomas se apresentam em torno de 30 minutos ap?s o consumo do molusco contaminado; variando entre n?useas, dores abdominais, v?mitos e diarr?ia. Quando a ingest?o da toxina acontece em quantidades inferiores a 48 μg.g-1, os sintomas acima descritos n?o se desenvolvem, por?m, seu consumo continuado favorece o surgimento de tumores no trato gastrointestinal devido ao poder carcinog?nico do AO. Este estudo pretendeu detectar e quantificar a toxina diarr?ica AO em mexilh?es Perna perna coletados entre os meses de maio e dezembro de 2006, e a verifica??o da presen?a de microalgas potencialmente t?xicas, nas esta??es primavera/ver?o (setembro a dezembro de 2006). De maio a dezembro de 2006, mexilh?es (Perna perna) foram coletados e analisados quanto ? presen?a da ficotoxina AO. As microalgas foram coletadas entre setembro e dezembro de 2006, com aux?lio de uma rede de pl?ncton (de 20μm de malha) e Bomba Rule 2000. A identifica??o dos dinoflagelados foi realizada em microsc?pio biol?gico invertido. A detec??o do AO nos mexilh?es foi realizada por Cromatografia L?quida de Alta Efici?ncia com Detec??o Fluorim?trica (CLAE-DF). Os resultados cromatogr?ficos indicaram a presen?a da toxina AO em todas as amostras obtidas de mexilh?es, de maio a outubro de 2006, em baixas concentra??es. Nas an?lises do fitopl?ncton, as diatom?ceas foram o grupo mais representativo comparado aos dinoflagelados. As esp?cies Prorocentrum micans e P. gracile observadas n?o foram apontadas como produtoras de toxinas at? o momento. Dentre os dinoflagelados potencialmente t?xicos foram encontradas as esp?cies: Dinophysis acuminata, D. tripos, D. rotundata e D. fortii. Os resultados indicam a necessidade da elabora??o e aplica??o efetiva de um programa de controle higi?nico-sanit?rio dos moluscos assim como monitoramento do ambiente, objetivando acima de tudo a seguran?a ? sa?de p?blica.

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