Electrokinetics is a family of effects that induces motion of a liquid or a particle within a liquid in response to an external electric field. Using the intrinsic electrical properties of bacteria and of breast cancer cells, electrokinetics can be used to manipulate these particles for two different types of applications: tissue engineering and breast cancer detection. The first application studied the effects of electric fields on bacteria cells as well as calcium ions to potentially create a meniscus scaffold with hydroxyapatite ends for anchoring. In response to the electric field, calcium ions were able to deposit locally and simultaneously with cellulose growth. Bacteria cells were also studied to determine their response under an AC field. At low frequencies, bacteria demonstrated controlled movement caused by electroosmosis and dielectrophoresis with a net motion caused by a dielectrophoretic force. In the second application, the separation capabilities of different stages of breast cancer cells from the same cell line were tested using contactless dielectrophoretic (cDEP) devices. The electric field gradients in cDEP devices were altered to optimize selectivity and to determine an estimated membrane capacitance for each. From the results, the membrane capacitance of the early to intermediate stages proved to be very similar; however, late stage breast cancer cells have potential in being separated from early and intermediate stages. / Master of Science

Work on individually constructed devices has demonstrated that nanowires (NWs) offer great promise for applications such as sensing and optoelectronics. Despite this work, reliable large scale alignment and integration of these individual nanostructures into a lithographically defined process remains a challenge. Dielectrophoresis (DEP) is a promising alignment method in which a nonuniform electric field is used to exert force on and manipulate NWs in solution. DEP offers the possibility of rapid, large area room-temperature assembly of NWs across opposing electrodes. DEP structures were fabricated on Si substrates and consisted of pairs of parallel Al electrodes on a 100nm insulating SiO2 film. ZnO NWs were suspended in isopropyl alcohol (IPA) and flowed across the electrodes. Alignment yield and angle of alignment were investigated as a function voltage and frequency. A method to remove excess nanowires through frequency tuning and IPA flushing is also investigated. The electrical properties of the formed ZnO NW devices will be reported. / Graduation date: 2010

Filtration is a technology that is used almost ubiquitously in society from uses raging from filtration of macroparticles from water to pharmaceutical grade filtration products to remove anything larger than a protein. However, with such a wide range of uses, most filtration products have the same issue; membrane clogging (fouling) that prevents continuous use and requires frequent maintenance. This thesis hypothesizes that by applying a direct current (DC) to an insulating array of posts, they will create a foul-less insulative dielectrophoretic filter (iDEP) that does not clog since particles will levitate above the insulating array. This thesis tested an inherited device (legacy device) and found that its design did not perform the desired foul-less filtration operation under the tested conditions. Therefore, using COMSOL simulations, the conditions of testing and improved deign were developed to fruition. These devices were fabricated and tested and found to successfully levitate yeast particles above the foul-less filtration array using a direct current insulative dielectrophoretic (iDEP) filter. Additionally, different post geometries were observed and how they affect the dielectric force on particles. Although a foul-less filter was not successfully developed over the course of this thesis, the groundwork for development of DC iDEP has been set.

Pathogens in drinking water are the cause of over 1.5 million deaths around the world every year, mostly in developing countries. Practical, cheap, and effective tools for detection of these pathogens are critical to advance public health in many areas around the globe. Micro electro-mechanical systems (MEMS) are miniaturized structures that can be used for a variety of purposes, including, but not limited to, small scale sensors. Therefore, MEMS can be used in place of expensive laboratory equipment and offer a cheap and practical tool for pathogen detection. The presented work's research objective is to introduce a new technique called embedded passivated-electrode insulator-based dielectrophoresis (EπDEP) for preconcentration, separation, or enrichment of bioparticles, including living cells. This new method combines traditional electrode-based DEP and insulator-based DEP with the objective of enhancing the electric field strength and capture efficiency within the microfluidic channel while alleviating direct contact between the electrode and the fluid. The EπDEP chip contains embedded electrodes within the microfluidic channel covered by a thin passivation layer of only 4 μm. The channel was designed with two nonaligned vertical columns of insulated microposts (200 μm diameter, 50 μm spacing) located between the electrodes (600 μm wide, 600 μm horizontal spacing) to generate the nonuniform electric field lines to concentrate cells while maintaining steady flow in the channel. The performance of the chip was demonstrated using Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial pathogens in aqueous media. Trapping efficiencies of 100% were obtained for both pathogens at an applied AC voltage of 50 V peak-to-peak and flow rates as high as 10 uL/min. / Master of Science

This work was focused on the characterization of microparticles with particular emphasis on waterborne pathogens which pose a great health risk to human lives. The goal of this study was to develop microfluidic systems for enhanced characterization and isolation of bioparticles. Insulator-based dielectrophoresis (iDEP) is a promising technique for analyzing, characterizing and isolation of microparticles based on their electrical properties. By employing insulator-based constrictions within the microchannel in combination with microelectrodes within the vicinity of the electrodes, dielectrophoretic performance is enhanced. In this study, three dimensional insulator-based dielectrophoresis devices are fabricated using our in-house developed 3D micromachining technique. This technology combines the benefits of electrode-based DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The dielectric properties of bacteria as well as submicron polystyrene beads are discussed and the impact of these results on the future development of iDEP microfluidic systems is explored. / Master of Science

This text describes different aspects of the design of a Doctor-on-a-Chip device. Doctor-on-a-Chip is a DNA analysis system integrated on a single chip, which should provide all of the advantages that stem from the system integration, such as small sample volume, fast and accurate analysis, and low cost. The text describes all of the steps of the on-chip sample analysis, including DNA extraction from the sample, purification, PCR amplification, novel dielectrophoretic sorting of the DNA molecules, and finally detection. The overview is given of the technologies which are available to make the integration on a single chip possible. The microfluidic technologies that are used to manipulate the sample and other chemical reagents are already known and in this text they are analyzed in terms of their feasibility in the on-chip system integration. These microfluidic technologies include, but are not limited to, microvalves, micromixers, micropumps, and chambers for PCR amplification. The novelty in the DNA analysis brought by Doctor-on-a-Chip is the way in which the different DNA molecules in the sample (for example, human and virus DNA) are sorted into different populations. This is done by means of dielectrophoresis – the force experienced by dielectric particles (such as DNA molecules) when subject to a non-uniform electric field. Different DNA molecules within a sample experience different dielectrophoretic forces within the same electric field, which makes their separation, and therefore detection, possible. In this text, the emphasis is put on numerical modelling of the dielectrophoretic effect on biological particles. The importance of numerical modelling lies in the fact that with the accurate model it is easier to design systems of microelectrodes for dielectrophoretic separation, and tune their sub-micrometre features to achieve the maximum separation efficacy. The numerical model described in this text is also experimentally verified with the novel microelectrodes design for dielectrophoretic separation, which is successfully used to separate the mixture of different particles in the micron and sub-micron range.

Biological sample analysis is a costly and time-consuming process. It involves highly trained technicians operating large and expensive instruments in a temperature and dust controlled environment. In the world of rising healthcare cost, the drive towards a more cost-effective solution calls for a point-of-care device that performs accurate analyses of human blood samples. To achieve this goal, today's bulky laboratory instruments need to be scaled down and integrated on a single microchip of only a few square centimeters or millimeters in size. Dielectrophoresis (DEP), a phenomenon where small particles such as human blood cells are manipulated by non-uniform electric fields, stands to feature prominently in the point-of-care device. An original device that enhances DEP effect through novel geometry of the electrodes is presented. When activated with two inverting sinusoidal waveforms, the novel-shaped electrodes generate horizontal bands of increasing electric fields on the surface of the microchip. With these bands of electric fields, particles can be manipulated to form a straight horizontal line at a predictable location. Experimental results showing the collection, separation, and transportation of mammalian cells are presented. A strategy for simultaneous processing of two or more types of particles is also demonstrated. With capabilities for an accurate position control and an increased throughput by parallel processing, the novel microchip device delivers substantial improvements over the existing DEP designs. The research presented here explores the effects of novel electrode geometries in cell manipulations and contributes to the overall progress of an automated blood analysis system.

Biological sample analysis is a costly and time-consuming process. It involves highly trained technicians operating large and expensive instruments in a temperature and dust controlled environment. In the world of rising healthcare cost, the drive towards a more cost-effective solution calls for a point-of-care device that performs accurate analyses of human blood samples. To achieve this goal, today's bulky laboratory instruments need to be scaled down and integrated on a single microchip of only a few square centimeters or millimeters in size. Dielectrophoresis (DEP), a phenomenon where small particles such as human blood cells are manipulated by non-uniform electric fields, stands to feature prominently in the point-of-care device. An original device that enhances DEP effect through novel geometry of the electrodes is presented. When activated with two inverting sinusoidal waveforms, the novel-shaped electrodes generate horizontal bands of increasing electric fields on the surface of the microchip. With these bands of electric fields, particles can be manipulated to form a straight horizontal line at a predictable location. Experimental results showing the collection, separation, and transportation of mammalian cells are presented. A strategy for simultaneous processing of two or more types of particles is also demonstrated. With capabilities for an accurate position control and an increased throughput by parallel processing, the novel microchip device delivers substantial improvements over the existing DEP designs. The research presented here explores the effects of novel electrode geometries in cell manipulations and contributes to the overall progress of an automated blood analysis system.

A further understanding of colloidal mixture behavior under applied electric fields would greatly benefit the design of smart material systems such as electrorheological fluidic devices and microfluidic reconfigurable antennas. This thesis presents a finite element analysis of colloidal mixture electrokinetic behavior. Computations of particle forces as a function of applied frequency and particle shape were performed. An effective medium property method was also studied. Fluidic and electric forces were obtained with various applied excitation frequencies throughout three locations in a coplanar microelectrode domain. This domain consists of two 50 nanometers thick gold electrodes separated by a 30 micrometers gap. The three locations are 1.2 micrometers, 40 micrometers, and 90 micrometers from the gap center. Total force vectors were computed by integrating Maxwell and Cauchy stress tensors to determine whether the particles are pushed toward or away from the electrode gap at frequencies of 10 Hz, 1 kHz, and 100 kHz. It was determined that particles were pushed outside the gap at median frequencies of 1kHz (indicating ac electroosmotic force domination) and began to be pushed back toward the gap at higher frequencies of 100 kHz (indicating dielectrophoretic force intensification). Particle shape effects were examined by calculating the electrical interparticle force between two particles at various incidences with respect to a uniform electric field. Particle attraction occurs when the line between the particle centers is aligned with the electric field; repulsion occurs when this center line is perpendicular. The incidence angle at which the particles switch from attraction to repulsion is defined as θcr. The aspect ratio and particle edge separation distances used in this study were 1, 5,12.92 and 0.25 micrometers, 0.50 micrometers, 2.0 micrometers, respectively. The results indicate that higher aspect ratio particles tend to have smaller θcr values and larger interparticle force magnitudes for given separation distances. Finally, effective dielectric constant simulations utilizing periodic crystalline arrangements of colloidal structure were performed. The results show good agreement with the Maxwell Garnett mixing rule at volume fractions above 30 percent. Less canonical structures of cubic particles were also modeled.

The objective of the research in this dissertation is to develop microsystem based separation technologies for whole cell cancer analysis using human whole blood as the input sample. This research work is carried out with two different approaches; one based on a miniaturized cascade magnetophoresis system and a second based on dielectrophoresis. The miniaturized systems can be fabricated using MEMS technologies combined with plastic fabrication techniques. The design, fabrication, packaging, and characterization of several versions of the magnetophoresis and dielectrophoresis microsystems for whole cell cancer analysis in human whole blood sample are presented. The developed magnetophoresis systems have demonstrated improved throughput in the removal of RBC from a human whole blood sample and its application to the separation of tagged cancer cells based on their surface expression level of a specific protein. The dielectrophoresis microsystem has successfully shown the ability to steer a blood stream between two outlets and to separate WBCs or cancer cells from a human whole blood sample. The developed microsystem based separation technologies can be further applied to the development of integrated system for cancer detection and treatments.