As the world population approaches seven billion, a greater strain is put on the resources necessary to sustain life. One of the most basic and essential resources is water and while two thirds of the earth is covered by water, the majority is either salt water (oceans and seas) or it is too contaminated to drink. The purpose of this project is to develop a portable device capable of testing whether a specific source of water (i.e. lake, river, well…) is potable. There are numerous filtration techniques that can remove contaminants and make even the dirtiest water clean enough for consumption but they are for the most part, very time consuming and immobile processes. The device is not a means of water purification but rather focuses on determining the content of the water and whether it is safe. Particles within the water are separated and trapped using a combination of a Taylor Couette fluid flow system and Dielectrophoretic electrodes. This paper explores Taylor Couette flow in a large gap and low aspect ratio system through theory and experimentation with early stage prototypes. Different inner cylinder radii, 2.12cm, 1.665cm and 1.075cm, were tested at different speeds approaching, at and passing the critical Taylor number, 3825, 4713 and 6923 respectively for each cylinder. Dielectrophoretic (DEP) electrodes were designed, fabricated, coated and tested using latex beads to determine the method of integrating them within the fluid flow system. Taylor Couette theory, in terms of the formation of vortices within the large gap, small aspect ratio system, was not validated during testing. The flow pattern generated was more akin to a chaotic circular Couette flow but still served to move the particles toward the outer wall. Fully integrated tests were run with limited success. Recommendations were made to pursue both circular Couette flow as the basis for iv particle separation and dimensional changes in the setup to allow for the formation of Taylor vortices by increasing the radius ratio but still allowing for a larger volume of fluid.

This research investigates the role of ABO blood type of erythrocytes in their dielectrophoretic response. The dielectrophoresis of erythrocytes of positive ABO blood types was studied at 5 V (peak to peak) and 1 MHz frequency AC field. The study revealed that the ABO blood type had an influence on the dielectrophoretic motion of the erythrocytes, particularly separating AB+ and O+ blood types. This is of particular significance since AB+ is a universal acceptor and O+ is a universal donor for blood transfusion purposes. The influence of field parameters, namely field strength and frequency of the AC field, was also studied for erythrocytes of positive ABO blood types. This research revealed that erythrocytes of each blood type respond differently at various frequencies and field strengths.

No description available.

Microfluidics has found numerous applications ranging from the life sciences industries for pharmaceuticals and biomedicine (drug design, delivery and detection, diagnostic devices) to industrial applications of combinational synthesis (such as rapid analysis and high throughput screening). Among all these, one of the intriguing exploitation of microfluidics or micro total analysis systems (µTAS) is the separation of circulating tumor cells (CTCs) from body fluids. Cancer cells spread from the initial site of a tumor by first invading the surrounding tissue, then by entering the blood or lymph vessels, and finally by crossing the vessel wall to exit the vasculature into distal organs. The September 2006 issue of the Journal of the National Cancer Institute (NCI) states: "The war on cancer was declared 40 years ago and cancer is still here," and "Technologies that capture enemy CTCs for further interrogation might prove useful in the war on cancer." CTCs cannot only become a new marker for cancer prognosis, but their detection can also be a valid new parameter for diagnosing cancer early, for monitoring disease progression and relapse, and for optimizing therapy. This research established a new method to manipulate rare cell types based on their electrical signatures using dielectrophoresis (DEP) without having direct contact between the electrodes and the sample, known as contactless dielectrophoresis (cDEP). DEP is the motion of a particle in a suspending medium due to its polarization in the presence of a non-uniform electric field. cDEP relies upon reservoirs filled with highly conductive fluid to act as electrodes and provide the necessary electric field. These reservoirs are placed adjacent to the main microfluidic channel and are separated from the sample by a thin barrier of a dielectric material as is shown in Figure 1h. The application of a high-frequency electric field to the electrode reservoirs causes their capacitive coupling to the main channel and an electric field is induced across the sample fluid. Similar to traditional DEP, cDEP exploits the varying geometry of the electrodes to create spatial non-uniformities in the electric field. However, by utilizing reservoirs filled with a highly conductive solution, rather than a separate thin film array, the electrode structures employed by cDEP can be fabricated in the same step as the rest of the device; hence the process is conducive to mass production. We demonstrated the ability to isolate human leukemia cancer cells (THP-1) cells from a heterogeneous mixture of live and dead cells using cDEP with more than 99% selectivity and 95% removal efficiency. Through numerical and experimental investigations, new generation of cDEP devices have been designed and tested to detect and isolate THP-1 cells from spiked blood samples with high selectivity and cell capture efficiency. Our experimental observations, using prototype devices, indicate that breast cancer cell lines at their different stages (MCF-7, MCF-10, and MDA-MB231) have unique electrical. Furthermore, through collaborations at the Wake Forest Comprehensive Center, we demonstrated that prostate tumor initiating cells (TICs) exhibit unique electrical signatures and DEP responses and cDEP technology can be exploited to isolate and enrich TICs for further genetic pathways investigations. / Ph. D.

Cancer metastasis is the leading cause of death related to cancer diseases. Once the cancer cells depart the primary tumor site and enter the blood circulation, they spread through the body and will likely initiate a new tumor site. Therefore, understanding the cell migration and stopping the spread in the initial stage is the utmost of importance. In this dissertation, we have proposed a 3-D microenvironment that (partially) mimics the structures, complexity and circulation of human organs for cell migration studies. We have developed the tools to fabricate 3-D complex geometries in PDMS from our previously developed single-mask, single-etch technology in silicon. In this work, 3-D patterns are transferred from silicon structures to glass following anodic bonding and high temperature glass re-flow processes. Silicon is etched back thoroughly via wet etching and the glass is used as master device to create 3-D PDMS structures for use in dielectrophoresis cell sorting applications. Furthermore, this work has been modified to fabricate 3-D master devices in PDMS to create 3-D structures in collagen hydrogels to mimic native tissue structures. We have studied the interaction of endothelial cells with model geometries of blood vessels in collagen hydrogel at different concentrations to mimic the biomechanical properties of tissues varying from normal to tumor under the growth factor stimulation. Finally, we have designed and fabricated a silicon-based transmigration well with a 30um-thick membrane and 8um pores. This platform includes a deep microfluidic channel on the back-side sealed with a glass wafer. The migratory behavior of highly metastatic breast cancer cells, MDA-MB-231, is tested under different drug treatment conditions. This versatile platform will enable the application of more complex fluidic circulation profile, enhanced integration with other technologies, and running multiple assays simultaneously. / Ph. D.

We developed a quantitative measurement technique using atomic force microscopy (AFM) to study the effects of both DC and AC external electric fields on the forces between two dielectric microspheres. In this work we measured the DC and AC electric field-induced forces and adhesion force between two barium titanate (BaTiO?) glass microspheres in a low humidity environment by this technique. The objective here is to find out the correlation between these measured forces and applied field strength, frequency, and the separation distance between the two spheres was studied. Since the spheres would oscillate under an AC field, the AC field-induced force was divided into dynamic component (i.e., time-varying term) and static component (i.e., time-averaged term) to investigate. The oscillatory response occurs at a frequency that is twice the drive frequency since the field-induced force is theoretically proportional to the square of the applied field. This behavior can be observed in the fast Fourier transformation (FFT) spectra of the time series of the deflection signal. The magnitude of the vibration response increases when the frequency of the drive force is near resonant frequency of the particle-cantilever probe. The amplitude of this vibration increases with proximity of the two particles, and ultimately causes the particles to repeatedly hit each other as in tapping mode AFM. The effect of the Maxwell-Wagner interfacial relaxation on the DC electric field-induced force was discovered by monitoring the variation of the field-induced force with time. The static component of the AC electric field-induced force does not vary with the applied frequency in the range from 1 to 100 kHz, suggesting that the crossover frequency may equal to or less than 1 kHz and the permittivities of the BaTiO? glass microspheres and medium dominate the field-3 induced force. The AC field-induced force is proportional to the square of the applied electric field strength. This relationship persists even when the separation between the spheres is much smaller than the diameter of the microspheres. The large magnitude of the force at small separations suggests that the local field is distorted by the presence of a second particle, and the continued dependence on the square of the field but the measured force is much larger than the theoretical results, suggesting that the local electric field around the closely spaced spheres is distorted and enhanced but the effects of the local field distortion may have not much to with the applied electric field. Compared with the calculated results from different models, our results demonstrate that the field-induced force is much more long-range than expected in theory. In addition, the DC field-induced adhesion force is larger than the AC field-induced one due to the interfacial charge accumulation, agreeing with the discovery of the Maxwell-Wagner interfacial relaxation effect on the DC field-induced force. No obvious correlation between the field-induced adhesion and the applied frequency is found. However, both the DC and AC field-induced adhesion forces display the linearity with the square of the applied electric field strength as well. / Master of Science

Nanobelts are a new class of semiconducting metal oxide nanowires with great potential for nanoscale devices. The present research focuses on the manipulation of SnO₂ nanobelts suspended in ethanol using microfluidics and electric fields. Dielectrophoresis (DEP) was demonstrated for the first time on semiconducting metal oxide nanobelts, which also resulted in the fabrication of a multiple nanobelt device. Detailed and direct real-time observations of the wide variety of nanobelt motions induced by DEP forces were conducted using an innovative setup and an inverted optical microscope. High AC electric fields were generated on a gold microelectrode (~ 20 µm gap) array, patterned on glass substrate, and covered by a ~ 10 µm tall PDMS (polydimethylsiloxane) channel, into which the nanobelt suspension was introduced for performing the DEP experiments. Negative DEP (repulsion) of the nanobelts was observed in the low frequency range (< 100 kHz) of the applied voltage, which caused rigid body motion as well as deformation of the nanobelts. In the high frequency range (~ 1 MHz - 10 MHz), positive DEP (attraction) of the nanobelts was observed. Using a parallel plate electrode arrangement, evidence of electrophoresis was also found for DC and low frequency (Hz) voltages. The existence of negative DEP effect is unusual considering the fact that if bulk SnO₂ conductivity and permittivity values are used in combination with ethanol properties to calculate the Clausius Mossotti factor using the simple dipole approximation theory; it predicts positive DEP for most of the frequency range experimentally studied. A fluidic nanobelt alignment technique was studied and used in the fabrication of single nanobelt devices with small electrode gaps. These devices were primarily used for conducting impedance spectroscopy measurements to obtain an estimate of the nanobelt electrical conductivity. Parametric numerical studies were conducted using COMSOL Multiphysics software package to understand the different aspects of the DEP phenomenon in nanobelts. The DEP induced forces and torques were computed using the Maxwell Stress Tensor (MST) approach. The DEP force on the nanobelt was calculated for a range of nanobelt conductivity values. The simulation results indicate that the experimentally observed behavior can be explained if the nanobelt is modeled as having two components: an electrically conductive interior and a nonconductive outer layer surrounding it. This forms the basis for an explanation of the negative DEP observed in SnO₂ nanobelts suspended in ethanol. It is thought that the nonconductive layer is due to depletion of the charge carriers from the nanobelt surface regions. This is consistent with the fact that surface depletion is a commonly observed phenomenon in SnO₂ and other semiconducting metal oxide materials. The major research contribution of this work is that, since nanostructures have large surface areas, surface dominant properties are important. Considering only bulk electrical properties can predict misleading DEP characteristics.

Thesis (MSc)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: The primary circuit components of very high temperature reactors (VHTRs) experience various unwanted fission products such as Kr, Xe, I, Cs, Sr, and Ag. These particle are generated during normal operation of the reactor from abaration, cracks and/or deffects are transported by the helium coolant. The main candidate that has been identified as a cause for concern and the focus of research to minimizing radioactive contamination of the reactor coolant circuit is silver. This is because the design of the coated particles limits the release of fission products into the coolant except for silver(Ag110m). Ag110m is a long lived metallic fission product formed inside the nuclear reactor core and is the only known element released out of the coated particles into the coolant at any temperature above 1150 ◦C when the reactor starts to heat up. The release occurs on intact coated particles, failed particles and also from defective particles. The amount of released silver is initially small and occurs as the pebble heats up and this is strongly dependent on the temperature of the core. It is therefore able to reach the surface of the reactor core and enter into the Helium coolant flowing throughout the reactor. Thus Ag110m will be circulated through the reactor circuit until it reaches the cooler sides of the main power system (MPS) where it will start to plate out. The presence of this radioactive silver in the primary circuit components may result in unwanted maintenance problems from a radiation hazard point of view. The development of a method to remove particles from the helium stream is therefore needed. In this work, two theoretical deflection models used to deflect the silver particles are proposed, namely the stochastic and the deterministic deflection models. The latter describes the deflection of microparticles in a helium medium. It uses the dielectrophoresis (DEP) technique to investigate the deflection of a silver µm moving in a helium medium with the bulk velocity of 0.021 ms−1 and subjected to a dielectrophoretic force only deflect an amount of 0.52039 nm and 4.49882 nm in the x - and z -directions on average. The former (stochastic deflection model) describes the deflection of ions and polarized particles by using probability theory, namely kinetic theory of gases. This model showed that the mean free time that the particle spends while deflected by a uniform electric field is short so much that there is not enough time for a silver particle to be appreciably deflected between collisions. For example, when an electric field of 100kV/m was applied on a single silver ion for a time of 0.1 µs, the deflection distance obtained was 33.38 mm for a free time of 0.189285 ns and under pressure and temperature conditions of 1 bar and 20◦ C. The Brownian motion was then compared to the effects of a nonuniform electric field in polarizing and deflecting an atom. This is done by comparing the Brownian motion and the polarizibility of an atom using nonuniform electric fields. It is found that the silver speed produced from Brownian motion (79.563 ms−1) is far larger than that produced from the polarizibility of an atom (4.69455×106 nms−1). The deterministic and stochastic deflection models using nonuniform electric fields proved that the dielectrophoresis technique is negligibly small in deflecting particles and cannot be used to deflect silver particles as required in a VHTR. / AFRIKAANSE OPSOMMING: Die primˆere siklus komponente van baie ho temperatuur reaktore (VHTRs) word bloodgestel aan verskeie ongewenste fisie produkte soos Kr, Xe, I, Cs, Sr, en Ag. Hierdie deeltjies word gegenereer gedurende normale werking van die reaktor van abarasie, krake en / of defekte word vervoer deur die helium verkoelingsmiddel. Die belangrikste kandidaat wat gedentifiseer is as ’n rede vir kommer en die fokus van navorsing op die minimalisering van radioaktiewe besoedeling van die reaktor verkoelingsmiddel siklus is silwer. Die rede hiervoor is die ontwerp van die omhulsel wat die vrylating van die fisie produkte in die koelmiddel behalwe vir silwer (Ag110m) beperk. Ag110m is ’n metaal fisie-produk met ’n lang leeftyd wat gevorm word binne-in die kern van die reaktor en is sover bekend die enigste element wat vrygestel word deur die bedekte deeltjies in die verkoelingsmiddel by enige temperatuur bo 1150 ◦C wanneer die reaktor begin verhit. Die vrystelling kom voor by ongeskonde brandstofomhulse, nie funksionele deeltjies en ook van gebrekkige deeltjies. Die bedrag van vrygestel silwer is aanvanklik klein en kom voor as die brandstofelemente verhit en heirdie vrystelling is sterk afhanklik van die temperatuur van die kern. Dit is dus in staat om die oppervlak van die reaktor kern te bereik en betree die Helium verkoelingsmiddel vloeistelsel en beweeg regdeur die reaktor. Dus sirkuleer die Ag110m deur die reaktor kring totdat dit die koeler kante van die MPS bereik waar dit sal begin uitplatteer. Die teenwoordigheid van hierdie radioaktiewe silwer in die primˆere stroombaan komponente kan lei tot ongewenste onderhoud probleme van ’n straling gevaar oogpunt. Die ontwikkeling van ’n metode om deeltjies te verwyder uit die helium stroom is dus nodig. In hierdie werk word van twee teoretiese defleksie modelle gebruik gemaak om die silwer partikels se defleksie te beskryf, naamlik die stogastiese en die deterministiese defleksie modelle. Laasgenoemde beskryf die defleksie van mikro grootte partikel in ’n helium medium. Dit maak gebruik van die dielektroflorosensie (DEP) tegniek om ondersoek in te stel na die defleksie van ’n silwer deeltjie met ’n radius van 3 μm. Dit is vanaf hierdie model waargeneemdat ’n silwer mikrodeeltjie met ’n radius van 3 m in ’n helium medium beweeg met die snelheid van 0,021 ms−1 en onderworpe is aan ’n dielektroforetiese krag dit net met ’n gemiddelde van 0,52039 nm en 4,49882 nm in die x - en z -rigtings deflekteer. Die voormalige (stogastiese defleksie model) beskryf die defleksie van ione en gepolariseerde partikels deur gebruik te maak van waarskynlikheidsteorie, naamlik die kinetiese teorie van gasse. Hierdie model toon dat die gemiddelde vrye tyd wat die deeltjie spandeer terwyl dit gedeflekteer word deur ’n uniforme elektriese veld sovel korter is dat daar nie genoeg tyd is vir ’n silwer deeltjie is om aansienlik tussen botsings gedeflekteer kan word nie. Byvoorbeeld, wanneer ’n elektriese veld van 100kV/m toegepas word op ’n enkele silwer ioon vir ’n tyd van 0.1 μs, die defleksie afstand van 33,38 mm verkry word vir ’n vrye tyd van 0.189285 ns en onder druk en temperatuur voorwaardes van 1 bar en 20 ◦C. Die Brown-beweging was dan vergeleke met die uitkoms van n univorme elektriese veld wat n polariserende atoom deflekteer. Dit word gedoen deur die vergelykings van die Brown-beweging en die polariseerbaarheid van ’n atoom met behulp van nie-uniform elektriese veld te gebruik. Daar word gevind dat as die silwer spoed van Brown se beweging (79,563 ms−1) veel groter is as di van die polariseerbare atoom (4,69455 × 10−6 nms−1). Die deterministiese en stogastiese defleksie modelle deur gebruik te maak van nonuniform elektriese velde bewys dat dielectrophoresis tegniek is weglaatbaar klein in defleksie van deeltjies en kan dus nie gebruik word om silwer partikels te buig soos wat in ’n VHTR vereis word nie.

This work focuses on the integration of microfluidics and dielectrophoresis(DEP) with the principles of field flow fractionation (FFF) to create a continuous-flow isolator for rare and viable circulating tumor cells (CTCs) from peripheral blood mononuclear cells (PBMNs) drawn from cancer patients. The method exploits differences in the plasma membrane capacitances of tumor and blood cells, which correspond to differences in the membrane surface areas of these cell types. DEP-FFF was first adapted to measure cell membrane capacitance, cell density and deformability profiles of cell populations. These properties of the NCI-60 panel of cancer cell types, which represents the wide functional diversity of cancers from 9 organs and leukemia, were compared with the normal cell subpopulations of peripheral blood. In every case, the NCI-60 cells exhibited membrane capacitance characteristics that were distinct from blood and, as a result, they could be isolated from blood by DEP. The heightened cancer cell membrane capacitances correlated strongly with membrane-rich morphological characteristics at their growth sites, including cell flattening, dendritic projections, and surface wrinkling. Following harvest from culture and maintenance in suspension, cancer cells were found to shed cytoplasm and membrane area over time and the suspended cell populations developed considerable morphological diversity. The shedding changed the cancer cell DEP properties but they could still be isolated from blood cells. A similar shedding process in the peripheral blood could account for the surprisingly wide morphological diversity seen among circulating cells isolated from clinical specimens. A continuous flow DEP-FFF method was devised to exploit these findings by allowing CTCs to be isolated from the nucleated cells of 10 mL clinical blood specimens in 40 minutes, an extremely high throughput rate for a microfluidic-based method. Cultured cancer cells could be isolated at 70-80% efficiency using this approach and the isolation of CTCs from clinical specimens was demonstrated. The results showed that the continuous DEP-FFF method delivers unmodified, viable CTCs for analysis, is perhaps universally applicable to isolation of CTCs from different cancer types and is independent of surface antigens - making it suitable for cells lacking the epithelial markers used in currently accepted CTC isolation methods. / text

Portable energy storage devices, which drive advanced technological devices, are improving the productivity and quality of our everyday lives. In order to meet the growing needs for energy storage in transportation applications, the current lithium-ion (Li-ion) battery technology requires new electrode materials with performance improvements in multiple aspects: (1) energy and power densities, (2) safety, and (3) performance lifetime. While a number of interesting nanomaterials have been synthesized in recent years with promising performance, accurate capabilities to probe the intrinsic performance of these high-performance materials within a battery environment are lacking. Most studies on electrode nanomaterials have so far used traditional, bulk-scale techniques such as cyclic voltammetry, electrochemical impedance spectroscopy, and Raman spectroscopy. These approaches give an ensemble-average estimation of the electrochemical properties of a battery electrode and does not provide a true indication of the performance that is intrinsic to its material system. Thus, new techniques are essential to understand the changes happening at a single particle level during the operation of a battery. The results from this thesis solve this need and study the electrical, mechanical and size changes that take place in a battery electrode at a single particle level. Single nanowire lithium cells are built by depositing nanowires in carefully designed device regions of a silicon chip using Dielectrophoresis (DEP). This work has demonstrated the assembly of several NW cathode materials like LiFePO4, pristine and acid-leached α-MnO2, todorokite – MnO2, acid and nonacid-leached Na0.44MnO2. Within these materials, α-MnO2 was chosen as the model material system for electrochemical experiments. Electrochemical lithiation of pristine α-MnO2 was performed inside a glove box. The volume, elasticity and conductivity changes were measured at each state-of-charge (SOC) to understand the performance of the material system. The NW size changes due to lithiation were measured using an Atomic Force Microscope (AFM) in the tapping mode. Electronic conductivity changes as a function of lithiation was also studied in the model α-MnO2 NWs and was found to decrease substantially with lithium loading. In other measurements involving a comparison between the alpha and todorokite phases of this material system, it was observed that the rate capability of these materials is limited not by the electronic but, by the ionic conductivity. Mechanical degradation of a battery cathode represents an important failure mode, which results in an irreversible loss of capacity with cycling. To analyze and understand these degradation mechanisms, this thesis has tested the evolution of nanomechanical properties of a battery cathode. Specifically, contact-mode AFM measurements have focused on the SOC-dependent changes in the Young’s modulus and fracture strength of an α-MnO2 NW electrode, which are critical parameters that determine its mechanical stability. These changes have been studied at the end of the first discharge step, 1 full electrochemical cycle, and 20 cycles. The observations show an increase in Young’s modulus at low concentrations of lithium loading and this is attributed to the formation of new Li-O bonds within the tunnel-structured cathode. As the lithium loading increases further, the Young’s modulus was observed to reduce and this is hypothesized to occur due to the distortions of the crystal at high lithium concentrations. The experimental-to-theoretical fracture strength ratio, which points to the defect density in the crystal at a given stoichiometry, was observed to reduce with electrochemical lithium insertion / cycling. This capability has demonstrated lithiation-dependent mechanical property measurements for the first time and represents an important contribution since degradation models, which are currently in use for materials at any size scale, always assume constant values regardless of the change in stoichiometry.